Restriction map for
pF1KB0962
(Circular) (Six-base) MAPSORT of: pF1KB0962.seq Check: 4957 from: 1 to: 4388
pF1KB0962 4388 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1154 2213 2645 3392 3660 832
Size: 322 1059 432 747 268 1560
Fragments arranged by size:
1560 1059 747 432 322 268
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 846 1693 3260 846
Size: 847 1567 1974
Fragments arranged by size:
1974 1567 847
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 658 696 982 1392 3741 3997 658
Size: 38 286 410 2349 256 1049
Fragments arranged by size:
2349 1049 410 286 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 393 1114 1408 393
Size: 721 294 3373
Fragments arranged by size:
3373 721 294
BamHI G'GATC_C
Cuts at: 647 1413 647
Size: 766 3622
BanI G'GyrC_C
Cuts at: 398 1404 1818 2066 2101 398
Size: 1006 414 248 35 2685
Fragments arranged by size:
2685 1006 414 248 35
BanII G_rGCy'C
Cuts at: 1115 1276 1319 1402 2432 1115
Size: 161 43 83 1030 3071
Fragments arranged by size:
3071 1030 161 83 43
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 606 820 1294 606
Size: 214 474 3700
Fragments arranged by size:
3700 474 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 524 490
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 1275 2286 3047 4388 1275
Size: 1011 761 1341 1275
Fragments arranged by size:
1341 1275 1011 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BfrBI ATG'CAT
Cuts at: 224 224
Size: 4388
BglI GCCn_nnn'nGGC
Cuts at: 837 837
Size: 4388
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 206 2013 2106 3162 206
Size: 1807 93 1056 1432
Fragments arranged by size:
1807 1432 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 943 982 868
Size: 75 39 4274
Fragments arranged by size:
4274 75 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1564 2443 2935 3233 775
Size: 789 879 492 298 1930
Fragments arranged by size:
1930 879 789 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 700 703 1327 700
Size: 3 624 3761
Fragments arranged by size:
3761 624 3
BseYI C'CCAG_C
Cuts at: 723 1064 1207 1813 2571 3148 3644 723
Size: 341 143 606 758 577 496 1467
Fragments arranged by size:
1467 758 606 577 496 341 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 891 385
Size: 506 3882
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1276 1319 1402 2180 2370 3162 1276
Size: 43 83 778 190 792 2502
Fragments arranged by size:
2502 792 778 190 83 43
Bsp1286I G_dGCh'C
Cuts at: 206 1115 1276 1319 1402 2013 2106 2180 2370
Size: 909 161 43 83 611 93 74 190
Cuts at: 2370 2432 3162 206
Size: 62 730 1432
Fragments arranged by size:
1432 909 730 611 190 161 93 83
74 62 43
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 729 3564 729
Size: 2835 1553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 2386 2567 2776 2386
Size: 181 209 3998
Fragments arranged by size:
3998 209 181
BsrGI T'GTAC_A
Cuts at: 597 597
Size: 4388
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 828 1765 828
Size: 937 3451
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 4388
BstYI r'GATC_y
Cuts at: 1 542 647 1413 1880 1903 2238 2484 3496
Size: 541 105 766 467 23 335 246 1012
Cuts at: 3496 3582 3594 3940 1
Size: 86 12 346 449
Fragments arranged by size:
1012 766 541 467 449 346 335 246
105 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 1631 1631
Size: 4388
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DraIII CAC_nnn'GTG
Cuts at: 595 1333 595
Size: 738 3650
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 110 1973 2147 2538 2565 3910 110
Size: 1863 174 391 27 1345 588
Fragments arranged by size:
1863 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 875 1054 1173 2411 2621 875
Size: 179 119 1238 210 2642
Fragments arranged by size:
2642 1238 210 179 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1274 1317 1400 1274
Size: 43 83 4262
Fragments arranged by size:
4262 83 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 868 943 982 1154 2213 2645 3392 3660
Size: 36 75 39 172 1059 432 747 268
Cuts at: 3660 832
Size: 1560
Fragments arranged by size:
1560 1059 747 432 268 172 75 39
36
EcoNI CCTnn'n_nnAGG
Cuts at: 685 685
Size: 4388
EcoO109I rG'GnC_Cy
Cuts at: 1527 1527
Size: 4388
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
EcoRV GAT'ATC
Cuts at: 855 855
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3092 4071 1822
Size: 248 1022 979 2139
Fragments arranged by size:
2139 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 463 495 1102 1134 1245 1277 2230
Size: 32 79 32 607 32 111 32 953
Cuts at: 2230 2260 2262 2292 352
Size: 30 2 30 2448
Fragments arranged by size:
2448 953 607 111 79 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 112 2149 112
Size: 2037 2351
MslI CAynn'nnrTG
Cuts at: 2377 2504 2377
Size: 127 4261
MspA1I CmG'CkG
Cuts at: 843 1499 1813 2173 3186 3431 843
Size: 656 314 360 1013 245 1800
Fragments arranged by size:
1800 1013 656 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NsiI A_TGCA'T
Cuts at: 226 226
Size: 4388
NspI r_CATG'y
Cuts at: 392 831 1441 1941 2848 392
Size: 439 610 500 907 1932
Fragments arranged by size:
1932 907 610 500 439
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1090 1122 3527 3559 1090
Size: 32 2405 32 1919
Fragments arranged by size:
2405 1919 32 32
PstI C_TGCA'G
Cuts at: 179 1131 1435 2120 179
Size: 952 304 685 2447
Fragments arranged by size:
2447 952 685 304
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 843 1813 2173 843
Size: 970 360 3058
Fragments arranged by size:
3058 970 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1276 1319 1402 1276
Size: 43 83 4262
Fragments arranged by size:
4262 83 43
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 1054 2411 2621 1054
Size: 1357 210 2821
Fragments arranged by size:
2821 1357 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SexAI A'CCwGG_T
Cuts at: 623 623
Size: 4388
SfcI C'TryA_G
Cuts at: 33 175 1127 1431 2116 3109 3300 33
Size: 142 952 304 685 993 191 1121
Fragments arranged by size:
1121 993 952 685 304 191 142
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1116 1410 1116
Size: 294 4094
SmlI C'TyrA_G
Cuts at: 790 1543 2458 2950 3212 790
Size: 753 915 492 262 1966
Fragments arranged by size:
1966 915 753 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 107 467 1522 1876 107
Size: 360 1055 354 2619
Fragments arranged by size:
2619 1055 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 849 1971 2104 2563 2772 849
Size: 1122 133 459 209 2465
Fragments arranged by size:
2465 1122 459 209 133
TatI w'GTAC_w
Cuts at: 576 597 3903 576
Size: 21 3306 1061
Fragments arranged by size:
3306 1061 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4356
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 843 1883 843
Size: 1040 3348
XmaI C'CCGG_G
Cuts at: 1114 1408 1114
Size: 294 4094
XmnI GAAnn'nnTTC
Cuts at: 697 884 982 697
Size: 187 98 4103
Fragments arranged by size:
4103 187 98
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI