Restriction map for
pF1KB0960
(Circular) (Six-base) MAPSORT of: pF1KB0960.seq Check: 3296 from: 1 to: 4388
pF1KB0960 4388 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1076 1426 1076
Size: 350 4038
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 211 811 2213 2645 3392 3660 151
Size: 60 600 1402 432 747 268 879
Fragments arranged by size:
1402 879 747 600 432 268 60
AfeI AGC'GCT
Cuts at: 271 271
Size: 4388
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AhdI GACnn_n'nnGTC
Cuts at: 960 960
Size: 4388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 290 290
Size: 4388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1246 1278 1246
Size: 32 4356
AlwNI CAG_nnn'CTG
Cuts at: 1693 3260 1693
Size: 1567 2821
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 753 1392 3741 3997 753
Size: 639 2349 256 1144
Fragments arranged by size:
2349 1144 639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 254 687 950 1250 1408 254
Size: 433 263 300 158 3234
Fragments arranged by size:
3234 433 300 263 158
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 143 374 475 1404 1818 2066 2101 143
Size: 231 101 929 414 248 35 2430
Fragments arranged by size:
2430 929 414 248 231 101 35
BanII G_rGCy'C
Cuts at: 255 1057 1402 2432 255
Size: 802 345 1030 2211
Fragments arranged by size:
2211 1030 802 345
BbeI G_GCGC'C
Cuts at: 378 1822 2070 378
Size: 1444 248 2696
Fragments arranged by size:
2696 1444 248
BbsI GAAGACnn'nnnn_
Cuts at: 817 817
Size: 4388
BciVI GTATCCnnnnn_n'
Cuts at: 1093 2286 3047 4388 1093
Size: 1193 761 1341 1093
Fragments arranged by size:
1341 1193 1093 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 203 1500 203
Size: 1297 3091
Bme1580I G_kGCm'C
Cuts at: 2013 2106 3162 2013
Size: 93 1056 3239
Fragments arranged by size:
3239 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 1327 1381 1303
Size: 24 54 4310
Fragments arranged by size:
4310 54 24
Bpu10I CC'TnA_GC
Cuts at: 1208 1208
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1564 2443 2935 3233 1180
Size: 384 879 492 298 2335
Fragments arranged by size:
2335 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 113 968 4092 113
Size: 855 3124 409
Fragments arranged by size:
3124 855 409
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 375 1819 2067 2769 4265 375
Size: 1444 248 702 1496 498
Fragments arranged by size:
1496 1444 702 498 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 160
Size: 30 4358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 424 559 562 885 1189 1192 235
Size: 189 135 3 323 304 3 3431
Fragments arranged by size:
3431 323 304 189 135 3 3
BseYI C'CCAG_C
Cuts at: 369 1000 1813 2571 3148 3644 369
Size: 631 813 758 577 496 1113
Fragments arranged by size:
1113 813 758 631 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 994 746
Size: 248 4140
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 722 934 1402 2180 2370 3162 722
Size: 212 468 778 190 792 1948
Fragments arranged by size:
1948 792 778 468 212 190
Bsp1286I G_dGCh'C
Cuts at: 255 722 934 1057 1402 2013 2106 2180 2370
Size: 467 212 123 345 611 93 74 190
Cuts at: 2370 2432 3162 255
Size: 62 730 1481
Fragments arranged by size:
1481 730 611 467 345 212 190 123
93 74 62
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 1438 1652 1954 2335 374
Size: 1064 214 302 381 2427
Fragments arranged by size:
2427 1064 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 2680 2734 4218 4383 50
Size: 157 2473 54 1484 165 55
Fragments arranged by size:
2473 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 2386 2567 2776 2386
Size: 181 209 3998
Fragments arranged by size:
3998 209 181
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 337 337
Size: 4388
BstYI r'GATC_y
Cuts at: 1 506 883 1413 1880 1903 2238 2484 3496
Size: 505 377 530 467 23 335 246 1012
Cuts at: 3496 3582 3594 3940 1
Size: 86 12 346 449
Fragments arranged by size:
1012 530 505 467 449 377 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 157 157
Size: 4388
BtgI C'CryG_G
Cuts at: 107 107
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 1631 1631
Size: 4388
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 497 1973 2147 2538 2565 3910 497
Size: 1476 174 391 27 1345 975
Fragments arranged by size:
1476 1345 975 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 130 239 254 435 529 922 2411 2621 130
Size: 109 15 181 94 393 1489 210 1897
Fragments arranged by size:
1897 1489 393 210 181 109 94 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 131 2596 2906 3052 4198 131
Size: 2465 310 146 1146 321
Fragments arranged by size:
2465 1146 321 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 211 811 1303 1327 1381 2213 2645 3392
Size: 60 600 492 24 54 832 432 747
Cuts at: 3392 3660 151
Size: 268 879
Fragments arranged by size:
879 832 747 600 492 432 268 60
54 24
EcoO109I rG'GnC_Cy
Cuts at: 334 516 832 947 1527 334
Size: 182 316 115 580 3195
Fragments arranged by size:
3195 580 316 182 115
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 273 378 1822 2070 3092 4071 273
Size: 105 1444 248 1022 979 590
Fragments arranged by size:
1444 1022 979 590 248 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 681 713 946 978 1175 1207 1276 1308 2230
Size: 32 233 32 197 32 69 32 922
Cuts at: 2230 2260 2262 2292 681
Size: 30 2 30 2777
Fragments arranged by size:
2777 922 233 197 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 374 1818 2066 374
Size: 1444 248 2696
Fragments arranged by size:
2696 1444 248
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 3059 3243 296
Size: 2763 184 1441
Fragments arranged by size:
2763 1441 184
MscI TGG'CCA
Cuts at: 499 2149 499
Size: 1650 2738
MslI CAynn'nnrTG
Cuts at: 2377 2504 2377
Size: 127 4261
MspA1I CmG'CkG
Cuts at: 231 1499 1813 2173 3186 3431 231
Size: 1268 314 360 1013 245 1188
Fragments arranged by size:
1268 1188 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 375 1819 2067 375
Size: 1444 248 2696
Fragments arranged by size:
2696 1444 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NspI r_CATG'y
Cuts at: 919 1093 1207 1441 1941 2848 919
Size: 174 114 234 500 907 2459
Fragments arranged by size:
2459 907 500 234 174 114
PasI CC'CwG_GG
Cuts at: 972 972
Size: 4388
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PfoI T'CCnGG_A
Cuts at: 509 1095 509
Size: 586 3802
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 334 516 832 947 334
Size: 182 316 115 3775
Fragments arranged by size:
3775 316 182 115
PspXI vC'TCGA_Gb
Cuts at: 687 687
Size: 4388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 684 716 684
Size: 32 4356
PstI C_TGCA'G
Cuts at: 1122 1435 2120 1122
Size: 313 685 3390
Fragments arranged by size:
3390 685 313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 1813 2173 1813
Size: 360 4028
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SanDI GG'GwC_CC
Cuts at: 947 947
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 130 435 922 2411 2621 130
Size: 305 487 1489 210 1897
Fragments arranged by size:
1897 1489 487 305 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 1118 1431 2116 3109 3300 33
Size: 1085 313 685 993 191 1121
Fragments arranged by size:
1121 1085 993 685 313 191
SfoI GGC'GCC
Cuts at: 376 1820 2068 376
Size: 1444 248 2696
Fragments arranged by size:
2696 1444 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 256 952 1252 1410 256
Size: 696 300 158 3234
Fragments arranged by size:
3234 696 300 158
SmlI C'TyrA_G
Cuts at: 687 1195 1543 2458 2950 3212 687
Size: 508 348 915 492 262 1863
Fragments arranged by size:
1863 915 508 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1207 1441 1941 1207
Size: 234 500 3654
Fragments arranged by size:
3654 500 234
StyI C'CwwG_G
Cuts at: 107 1522 1876 107
Size: 1415 354 2619
Fragments arranged by size:
2619 1415 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1971 2104 2563 2772 1971
Size: 133 459 209 3587
Fragments arranged by size:
3587 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 3905 3937 160
Size: 32 3713 32 611
Fragments arranged by size:
3713 611 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 337 1883 337
Size: 1546 2842
XhoI C'TCGA_G
Cuts at: 687 687
Size: 4388
XmaI C'CCGG_G
Cuts at: 254 950 1250 1408 254
Size: 696 300 158 3234
Fragments arranged by size:
3234 696 300 158
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI