Restriction map for
pF1KB0958
(Circular) (Six-base) MAPSORT of: pF1KB0958.seq Check: 5789 from: 1 to: 4373
pF1KB0958 4373 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1411 1411
Size: 4373
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1078 2198 2630 3377 3645 889
Size: 189 1120 432 747 268 1617
Fragments arranged by size:
1617 1120 747 432 268 189
AflII C'TTAA_G
Cuts at: 1339 1339
Size: 4373
AflIII A'CryG_T
Cuts at: 1725 2829 3806 1725
Size: 1104 977 2292
Fragments arranged by size:
2292 1104 977
AgeI A'CCGG_T
Cuts at: 2761 2761
Size: 4373
AleI CACnn'nnGTG
Cuts at: 719 719
Size: 4373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 1678 3245 1678
Size: 1567 2806
ApaLI G'TGCA_C
Cuts at: 3143 3143
Size: 4373
ApoI r'AATT_y
Cuts at: 238 728 1377 3726 3982 238
Size: 490 649 2349 256 629
Fragments arranged by size:
2349 649 629 490 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 136 1393 136
Size: 1257 3116
BamHI G'GATC_C
Cuts at: 1398 1398
Size: 4373
BanI G'GyrC_C
Cuts at: 1389 1803 2051 2086 1389
Size: 414 248 35 3676
Fragments arranged by size:
3676 414 248 35
BanII G_rGCy'C
Cuts at: 1066 1387 2417 1066
Size: 321 1030 3022
Fragments arranged by size:
3022 1030 321
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BbsI GAAGACnn'nnnn_
Cuts at: 632 1267 632
Size: 635 3738
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3032 4373 2271
Size: 761 1341 2271
Fragments arranged by size:
2271 1341 761
BclI T'GATC_A
Cuts at: 1893 1893
Size: 4373
BglII A'GATC_T
Cuts at: 1888 3925 1888
Size: 2037 2336
BlpI GC'TnA_GC
Cuts at: 1485 1485
Size: 4373
Bme1580I G_kGCm'C
Cuts at: 1998 2091 3147 1998
Size: 93 1056 3224
Fragments arranged by size:
3224 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1585 1666 2001 4145 1585
Size: 81 335 2144 1813
Fragments arranged by size:
2144 1813 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1003 985
Size: 18 4355
Bpu10I CC'TnA_GC
Cuts at: 410 410
Size: 4373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1549 2428 2920 3218 1549
Size: 879 492 298 2704
Fragments arranged by size:
2704 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4077 4077
Size: 4373
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 158 1562 2083 2761 3035 3182 3593 158
Size: 1404 521 678 274 147 411 938
Fragments arranged by size:
1404 938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 456 486 456
Size: 30 4343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 477 1225 477
Size: 748 3625
BseYI C'CCAG_C
Cuts at: 393 824 1798 2556 3133 3629 393
Size: 431 974 758 577 496 1137
Fragments arranged by size:
1137 974 758 577 496 431
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3169 104
Size: 1811 46 1208 1308
Fragments arranged by size:
1811 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 920 1387 2165 2355 3147 920
Size: 467 778 190 792 2146
Fragments arranged by size:
2146 792 778 467 190
BsmBI CGTCTCn'nnnn_
Cuts at: 106 577 106
Size: 471 3902
Bsp1286I G_dGCh'C
Cuts at: 920 1066 1387 1998 2091 2165 2355 2417 3147
Size: 146 321 611 93 74 190 62 730
Cuts at: 3147 920
Size: 2146
Fragments arranged by size:
2146 730 611 321 190 146 93 74
62
BspEI T'CCGG_A
Cuts at: 3593 3593
Size: 4373
BspHI T'CATG_A
Cuts at: 509 861 3549 509
Size: 352 2688 1333
Fragments arranged by size:
2688 1333 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1637 1939 2320 1423
Size: 214 302 381 3476
Fragments arranged by size:
3476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2285 2285
Size: 4373
BsrFI r'CCGG_y
Cuts at: 2371 2552 2761 2371
Size: 181 209 3983
Fragments arranged by size:
3983 209 181
BssSI C'ACGA_G
Cuts at: 2644 3002 2644
Size: 358 4015
BstAPI GCAn_nnn'nTGC
Cuts at: 1750 1750
Size: 4373
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstYI r'GATC_y
Cuts at: 1 1398 1865 1888 2223 2469 3481 3567 3579
Size: 1397 467 23 335 246 1012 86 12
Cuts at: 3579 3925 1
Size: 346 449
Fragments arranged by size:
1397 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1015 107
Size: 908 3465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 1616 1616
Size: 4373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 186 221 186
Size: 35 4338
DraI TTT'AAA
Cuts at: 1372 1372
Size: 4373
DrdI GACnn_nn'nnGTC
Cuts at: 2079 2937 2079
Size: 858 3515
EaeI y'GGCC_r
Cuts at: 518 1958 2132 2523 2550 3895 518
Size: 1440 174 391 27 1345 996
Fragments arranged by size:
1440 1345 996 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 1054 1193 1339 2396 2606 1054
Size: 139 146 1057 210 2821
Fragments arranged by size:
2821 1057 210 146 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 2891 3037 4183 2581
Size: 310 146 1146 2771
Fragments arranged by size:
2771 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1385 1385
Size: 4373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 985 1003 1078 2198 2630 3377 3645 889
Size: 96 18 75 1120 432 747 268 1617
Fragments arranged by size:
1617 1120 747 432 268 96 75 18
EcoNI CCTnn'n_nnAGG
Cuts at: 682 1085 682
Size: 403 3970
EcoO109I rG'GnC_Cy
Cuts at: 1512 1512
Size: 4373
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
EcoRV GAT'ATC
Cuts at: 943 1026 943
Size: 83 4290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 570 1351 2392 240
Size: 330 781 1041 2221
Fragments arranged by size:
2221 1041 781 330
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 1807 2055 3077 4056 1807
Size: 248 1022 979 2124
Fragments arranged by size:
2124 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2215 2245 2247 2277 2215
Size: 30 2 30 4311
Fragments arranged by size:
4311 30 30 2
HincII GTy'rAC
Cuts at: 1412 1412
Size: 4373
HindIII A'AGCT_T
Cuts at: 879 1158 879
Size: 279 4094
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1174 3044 3228 1174
Size: 1870 184 2319
Fragments arranged by size:
2319 1870 184
MscI TGG'CCA
Cuts at: 520 2134 520
Size: 1614 2759
MslI CAynn'nnrTG
Cuts at: 439 514 719 1099 2362 2489 439
Size: 75 205 380 1263 127 2323
Fragments arranged by size:
2323 1263 380 205 127 75
MspA1I CmG'CkG
Cuts at: 1484 1798 2158 3171 3416 1484
Size: 314 360 1013 245 2441
Fragments arranged by size:
2441 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NcoI C'CATG_G
Cuts at: 107 107
Size: 4373
NdeI CA'TA_TG
Cuts at: 487 487
Size: 4373
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NspI r_CATG'y
Cuts at: 1426 1926 2833 1426
Size: 500 907 2966
Fragments arranged by size:
2966 907 500
PciI A'CATG_T
Cuts at: 2829 2829
Size: 4373
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 4341
PshAI GACnn'nnGTC
Cuts at: 488 488
Size: 4373
PsiI TTA'TAA
Cuts at: 1256 1256
Size: 4373
PstI C_TGCA'G
Cuts at: 483 648 1420 2105 483
Size: 165 772 685 2751
Fragments arranged by size:
2751 772 685 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 1798 2158 1798
Size: 360 4013
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1387 1387
Size: 4373
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 1054 2396 2606 1054
Size: 1342 210 2821
Fragments arranged by size:
2821 1342 210
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 184 1006 3890 184
Size: 822 2884 667
Fragments arranged by size:
2884 822 667
SfcI C'TryA_G
Cuts at: 33 479 644 1416 2101 3094 3285 33
Size: 446 165 772 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 446 191 165
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 1339 1528 2443 2935 3197 1339
Size: 189 915 492 262 2515
Fragments arranged by size:
2515 915 492 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 1426 1926 1426
Size: 500 3873
StyI C'CwwG_G
Cuts at: 107 788 1507 1861 107
Size: 681 719 354 2619
Fragments arranged by size:
2619 719 681 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1956 2089 2548 2757 1956
Size: 133 459 209 3572
Fragments arranged by size:
3572 459 209 133
TatI w'GTAC_w
Cuts at: 182 1004 3888 182
Size: 822 2884 667
Fragments arranged by size:
2884 822 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4341
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1868 1868
Size: 4373
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
XmnI GAAnn'nnTTC
Cuts at: 728 857 1298 728
Size: 129 441 3803
Fragments arranged by size:
3803 441 129
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI