Restriction map for
pF1KB0953
(Circular) (Six-base) MAPSORT of: pF1KB0953.seq Check: 8401 from: 1 to: 3665
pF1KB0953 3665 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 226 1490 1922 2669 2937 148
Size: 78 1264 432 747 268 876
Fragments arranged by size:
1264 876 747 432 268 78
AflIII A'CryG_T
Cuts at: 1017 2121 3098 1017
Size: 1104 977 1584
Fragments arranged by size:
1584 1104 977
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 432 492 970 2537 432
Size: 60 478 1567 1560
Fragments arranged by size:
1567 1560 478 60
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 116 425 679 1709 116
Size: 309 254 1030 2072
Fragments arranged by size:
2072 1030 309 254
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 481 558 2032 481
Size: 77 1474 2114
Fragments arranged by size:
2114 1474 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 409 518 553 292
Size: 117 109 35 3404
Fragments arranged by size:
3404 117 109 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 223
Size: 30 3635
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 457
Size: 3665
BseYI C'CCAG_C
Cuts at: 249 1090 1848 2425 2921 249
Size: 841 758 577 496 993
Fragments arranged by size:
993 841 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 278 358 425 504 679 1457 1647 2439 278
Size: 80 67 79 175 778 190 792 1504
Fragments arranged by size:
1504 792 778 190 175 80 79 67
Bsp1286I G_dGCh'C
Cuts at: 116 278 358 425 504 679 1290 1383 1457
Size: 162 80 67 79 175 611 93 74
Cuts at: 1457 1647 1709 2439 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 730 611 190 175 162 93 80
79 74 67 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 608 715 929 1231 1612 500
Size: 108 107 214 302 381 2553
Fragments arranged by size:
2553 381 302 214 108 107
BsrBI CCG'CTC
Cuts at: 50 150 1957 2011 3495 3660 50
Size: 100 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 1663 1844 2053 1663
Size: 181 209 3275
Fragments arranged by size:
3275 209 181
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 492 1042 492
Size: 550 3115
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 312 452 1250 1424 1815 1842 3187 312
Size: 140 798 174 391 27 1345 790
Fragments arranged by size:
1345 798 790 391 174 140 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 354 1873 2183 2329 3475 354
Size: 1519 310 146 1146 544
Fragments arranged by size:
1519 1146 544 310 146
EcoICRI GAG'CTC
Cuts at: 423 677 423
Size: 254 3411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 226 292 409 518 553 1490 1922 2669
Size: 78 66 117 109 35 937 432 747
Cuts at: 2669 2937 148
Size: 268 876
Fragments arranged by size:
937 876 747 432 268 117 109 78
66 35
EcoO109I rG'GnC_Cy
Cuts at: 489 528 804 489
Size: 39 276 3350
Fragments arranged by size:
3350 276 39
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 450 584 1099 1347 2369 3348 450
Size: 134 515 248 1022 979 767
Fragments arranged by size:
1022 979 767 515 248 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 1507 1537 1539 1569 223
Size: 32 1252 30 2 30 2319
Fragments arranged by size:
2319 1252 32 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 314 1426 314
Size: 1112 2553
MslI CAynn'nnrTG
Cuts at: 1654 1781 1654
Size: 127 3538
MspA1I CmG'CkG
Cuts at: 417 655 776 1090 1450 2463 2708 417
Size: 238 121 314 360 1013 245 1374
Fragments arranged by size:
1374 1013 360 314 245 238 121
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NcoI C'CATG_G
Cuts at: 107 107
Size: 3665
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 405 718 1218 2125 405
Size: 313 500 907 1945
Fragments arranged by size:
1945 907 500 313
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PfoI T'CCnGG_A
Cuts at: 530 623 530
Size: 93 3572
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PpuMI rG'GwC_Cy
Cuts at: 489 528 489
Size: 39 3626
PstI C_TGCA'G
Cuts at: 580 712 1397 580
Size: 132 685 2848
Fragments arranged by size:
2848 685 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 655 1090 1450 655
Size: 435 360 2870
Fragments arranged by size:
2870 435 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 425 679 425
Size: 254 3411
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 576 708 1393 2386 2577 33
Size: 543 132 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 191 132
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 405 718 1218 405
Size: 313 500 2852
Fragments arranged by size:
2852 500 313
StyI C'CwwG_G
Cuts at: 107 799 1153 107
Size: 692 354 2619
Fragments arranged by size:
2619 692 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 1248 1381 1840 2049 298
Size: 950 133 459 209 1914
Fragments arranged by size:
1914 950 459 209 133
TatI w'GTAC_w
Cuts at: 3180 3180
Size: 3665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
XmnI GAAnn'nnTTC
Cuts at: 211 211
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI