Restriction map for
pF1KB0950
(Circular) (Six-base) MAPSORT of: pF1KB0950.seq Check: 9595 from: 1 to: 4334
pF1KB0950 4334 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AfeI AGC'GCT
Cuts at: 401 401
Size: 4334
AflIII A'CryG_T
Cuts at: 1686 2790 3767 1686
Size: 1104 977 2253
Fragments arranged by size:
2253 1104 977
AgeI A'CCGG_T
Cuts at: 1142 2722 1142
Size: 1580 2754
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 1338 3687 3943 1338
Size: 2349 256 1729
Fragments arranged by size:
2349 1729 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 628 982 1354 628
Size: 354 372 3608
Fragments arranged by size:
3608 372 354
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 416 758 868 1350 1764 2012 2047 416
Size: 342 110 482 414 248 35 2703
Fragments arranged by size:
2703 482 414 342 248 110 35
BanII G_rGCy'C
Cuts at: 179 983 1348 2378 179
Size: 804 365 1030 2135
Fragments arranged by size:
2135 1030 804 365
BbeI G_GCGC'C
Cuts at: 762 1768 2016 762
Size: 1006 248 3080
Fragments arranged by size:
3080 1006 248
BbsI GAAGACnn'nnnn_
Cuts at: 158 158
Size: 4334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 963 997 963
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 877 958 877
Size: 81 4253
BglII A'GATC_T
Cuts at: 1091 1849 3886 1091
Size: 758 2037 1539
Fragments arranged by size:
2037 1539 758
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 659 873 1959 2052 3108 659
Size: 214 1086 93 1056 1885
Fragments arranged by size:
1885 1086 1056 214 93
BmrI ACTGGGnnnn_n'
Cuts at: 721 1068 1546 1627 1962 4106 721
Size: 347 478 81 335 2144 949
Fragments arranged by size:
2144 949 478 347 335 81
BmtI G_CTAG'C
Cuts at: 1276 1276
Size: 4334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
Bpu10I CC'TnA_GC
Cuts at: 188 188
Size: 4334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 990 1687 990
Size: 697 3637
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 759 890 1765 2013 2715 4211 759
Size: 131 875 248 702 1496 882
Fragments arranged by size:
1496 882 875 702 248 131
BsaWI w'CCGG_w
Cuts at: 1142 1523 2044 2722 2996 3143 3554 1142
Size: 381 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 381 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 622
Size: 4334
BseYI C'CCAG_C
Cuts at: 499 595 918 1759 2517 3094 3590 499
Size: 96 323 841 758 577 496 1243
Fragments arranged by size:
1243 841 758 577 496 323 96
BsiEI CG_ry'CG
Cuts at: 104 376 720 1876 1922 3130 104
Size: 272 344 1156 46 1208 1308
Fragments arranged by size:
1308 1208 1156 344 272 46
BsiHKAI G_wGCw'C
Cuts at: 1348 2126 2316 3108 1348
Size: 778 190 792 2574
Fragments arranged by size:
2574 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 761 761
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 179 659 873 983 1348 1959 2052 2126 2316
Size: 480 214 110 365 611 93 74 190
Cuts at: 2316 2378 3108 179
Size: 62 730 1405
Fragments arranged by size:
1405 730 611 480 365 214 190 110
93 74 62
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 1384 1598 1900 2281 233
Size: 1151 214 302 381 2286
Fragments arranged by size:
2286 1151 381 302 214
BsrBI CCG'CTC
Cuts at: 50 543 1245 2626 2680 4164 4329 50
Size: 493 702 1381 54 1484 165 55
Fragments arranged by size:
1484 1381 702 493 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 1106 1142 1166 2332 2513 2722 1106
Size: 36 24 1166 181 209 2718
Fragments arranged by size:
2718 1166 209 181 36 24
BssSI C'ACGA_G
Cuts at: 1180 2605 2963 1180
Size: 1425 358 2551
Fragments arranged by size:
2551 1425 358
BstAPI GCAn_nnn'nTGC
Cuts at: 560 1711 560
Size: 1151 3183
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstEII G'GTnAC_C
Cuts at: 405 405
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 954 1018 954
Size: 64 4270
BstYI r'GATC_y
Cuts at: 1 1091 1359 1826 1849 2184 2430 3442 3528
Size: 1090 268 467 23 335 246 1012 86
Cuts at: 3528 3540 3886 1
Size: 12 346 449
Fragments arranged by size:
1090 1012 467 449 346 335 268 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 449 1100 449
Size: 651 3683
BtgI C'CryG_G
Cuts at: 107 813 953 1011 1112 107
Size: 706 140 58 101 3329
Fragments arranged by size:
3329 706 140 101 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1031 2284 2465 2628 1031
Size: 1253 181 163 2737
Fragments arranged by size:
2737 1253 181 163
BtsI GCAGTG_nn'
Cuts at: 320 579 585 903 1577 320
Size: 259 6 318 674 3077
Fragments arranged by size:
3077 674 318 259 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 735 770 735
Size: 35 4299
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DraIII CAC_nnn'GTG
Cuts at: 1185 1185
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 195 373 728 1919 2093 2484 2511 3856 195
Size: 178 355 1191 174 391 27 1345 673
Fragments arranged by size:
1345 1191 673 391 355 178 174 27
EagI C'GGCC_G
Cuts at: 373 1919 373
Size: 1546 2788
EarI CTCTTCn'nnn_
Cuts at: 338 2357 2567 338
Size: 2019 210 2105
Fragments arranged by size:
2105 2019 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 889 1185 2542 2852 2998 4144 889
Size: 296 1357 310 146 1146 1079
Fragments arranged by size:
1357 1146 1079 310 296 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 139 516 139
Size: 377 3957
EcoO109I rG'GnC_Cy
Cuts at: 293 453 741 1473 293
Size: 160 288 732 3154
Fragments arranged by size:
3154 732 288 160
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 711 2353 711
Size: 1642 2692
FseI GG_CCGG'CC
Cuts at: 1110 1110
Size: 4334
FspI TGC'GCA
Cuts at: 909 2115 909
Size: 1206 3128
HaeII r_GCGC'y
Cuts at: 395 403 616 636 762 1026 1768 2016 3038
Size: 8 213 20 126 264 742 248 1022
Cuts at: 3038 4017 395
Size: 979 712
Fragments arranged by size:
1022 979 742 712 264 248 213 126
20 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2176 2206 2208 2238 2176
Size: 30 2 30 4272
Fragments arranged by size:
4272 30 30 2
HincII GTy'rAC
Cuts at: 1002 1373 1002
Size: 371 3963
KasI G'GCGC_C
Cuts at: 758 1764 2012 758
Size: 1006 248 3080
Fragments arranged by size:
3080 1006 248
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3189 3005
Size: 184 4150
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 1016 2323 2450 1016
Size: 1307 127 2900
Fragments arranged by size:
2900 1307 127
MspA1I CmG'CkG
Cuts at: 378 566 1445 1759 2119 3132 3377 378
Size: 188 879 314 360 1013 245 1335
Fragments arranged by size:
1335 1013 879 360 314 245 188
NaeI GCC'GGC
Cuts at: 1108 2515 1108
Size: 1407 2927
NarI GG'CG_CC
Cuts at: 759 1765 2013 759
Size: 1006 248 3080
Fragments arranged by size:
3080 1006 248
NcoI C'CATG_G
Cuts at: 107 953 107
Size: 846 3488
NgoMIV G'CCGG_C
Cuts at: 1106 2513 1106
Size: 1407 2927
NheI G'CTAG_C
Cuts at: 1272 1272
Size: 4334
NotI GC'GGCC_GC
Cuts at: 373 373
Size: 4334
NspI r_CATG'y
Cuts at: 666 1387 1887 2794 666
Size: 721 500 907 2206
Fragments arranged by size:
2206 907 721 500
PasI CC'CwG_GG
Cuts at: 435 512 435
Size: 77 4257
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 4334
PfoI T'CCnGG_A
Cuts at: 258 258
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PpuMI rG'GwC_Cy
Cuts at: 293 293
Size: 4334
PstI C_TGCA'G
Cuts at: 383 399 565 571 1091 1381 2066 383
Size: 16 166 6 520 290 685 2651
Fragments arranged by size:
2651 685 520 290 166 16 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 566 1759 2119 566
Size: 1193 360 2781
Fragments arranged by size:
2781 1193 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SanDI GG'GwC_CC
Cuts at: 293 293
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SexAI A'CCwGG_T
Cuts at: 1204 1204
Size: 4334
SfcI C'TryA_G
Cuts at: 33 379 395 561 567 1087 1377 2062 3055
Size: 346 16 166 6 520 290 685 993
Cuts at: 3055 3246 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 520 346 290 191 166
16 6
SfoI GGC'GCC
Cuts at: 760 1766 2014 760
Size: 1006 248 3080
Fragments arranged by size:
3080 1006 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SnaBI TAC'GTA
Cuts at: 990 990
Size: 4334
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 666 1387 1887 666
Size: 721 500 3113
Fragments arranged by size:
3113 721 500
StuI AGG'CCT
Cuts at: 277 277
Size: 4334
StyI C'CwwG_G
Cuts at: 107 179 410 947 953 1238 1468 1822 107
Size: 72 231 537 6 285 230 354 2619
Fragments arranged by size:
2619 537 354 285 231 230 72 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 513 822 1917 2050 2509 2718 513
Size: 309 1095 133 459 209 2129
Fragments arranged by size:
2129 1095 459 309 209 133
TatI w'GTAC_w
Cuts at: 3849 3849
Size: 4334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 3851 3883 103
Size: 32 3716 32 554
Fragments arranged by size:
3716 554 32 32
Tth111I GACn'n_nGTC
Cuts at: 892 2131 892
Size: 1239 3095
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 954 1829 210
Size: 744 875 2715
Fragments arranged by size:
2715 875 744
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BpmI BsaXI
BsgI BsiWI BsmI BsrGI BssHII BstZ17I ClaI EcoRV
FspAI HindIII HpaI MfeI NdeI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI