Restriction map for
pF1KB0946
(Circular) (Six-base) MAPSORT of: pF1KB0946.seq Check: 3150 from: 1 to: 4310
pF1KB0946 4310 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 502 1348 502
Size: 846 3464
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 1304 2135 2567 3314 3582 554
Size: 750 831 432 747 268 1282
Fragments arranged by size:
1282 831 750 747 432 268
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1615 3182 1615
Size: 1567 2743
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 430 438 450 540 570 630 770 1022 1190
Size: 8 12 90 30 60 140 252 168
Cuts at: 1190 1314 3663 3919 430
Size: 124 2349 256 821
Fragments arranged by size:
2349 821 256 252 168 140 124 90
60 30 12 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 1330 1330
Size: 4310
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 293 1324 2354 293
Size: 1031 1030 2249
Fragments arranged by size:
2249 1031 1030
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 820 820
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 263 1830 263
Size: 1567 2743
BfrBI ATG'CAT
Cuts at: 884 884
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 686 2677 686
Size: 1991 2319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 786 1136 786
Size: 350 3960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1486 2365 2857 3155 1216
Size: 270 879 492 298 2371
Fragments arranged by size:
2371 879 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 234 4014 234
Size: 3780 530
BsaAI yAC'GTr
Cuts at: 1663 1663
Size: 4310
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1735 2493 3070 3566 1735
Size: 758 577 496 2479
Fragments arranged by size:
2479 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 940 1324 2102 2292 3084 940
Size: 384 778 190 792 2166
Fragments arranged by size:
2166 792 778 384 190
BsmI GAATG_Cn'
Cuts at: 472 1222 472
Size: 750 3560
BsmBI CGTCTCn'nnnn_
Cuts at: 1091 1091
Size: 4310
Bsp1286I G_dGCh'C
Cuts at: 293 940 1324 1935 2028 2102 2292 2354 3084
Size: 647 384 611 93 74 190 62 730
Cuts at: 3084 293
Size: 1519
Fragments arranged by size:
1519 730 647 611 384 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 150 3486 150
Size: 3336 974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1360 1574 1876 2257 1360
Size: 214 302 381 3413
Fragments arranged by size:
3413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 1335 1802 1825 2160 2406 3418 3504 3516
Size: 1334 467 23 335 246 1012 86 12
Cuts at: 3516 3862 1
Size: 346 449
Fragments arranged by size:
1334 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 825 909 825
Size: 84 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 723 1553 723
Size: 830 3480
DraI TTT'AAA
Cuts at: 629 1309 629
Size: 680 3630
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 149 2333 2543 149
Size: 2184 210 1916
Fragments arranged by size:
2184 1916 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 786 1136 1304 2135 2567 3314 3582 554
Size: 232 350 168 831 432 747 268 1282
Fragments arranged by size:
1282 831 747 432 350 268 232 168
EcoO109I rG'GnC_Cy
Cuts at: 1449 1449
Size: 4310
EcoRI G'AATT_C
Cuts at: 540 770 1022 1190 1314 540
Size: 230 252 168 124 3536
Fragments arranged by size:
3536 252 230 168 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 1744 1992 3014 3993 1744
Size: 248 1022 979 2061
Fragments arranged by size:
2061 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 689 721 2152 2182 2184 2214 313
Size: 32 344 32 1431 30 2 30 2409
Fragments arranged by size:
2409 1431 344 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
HindIII A'AGCT_T
Cuts at: 1151 1151
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 551 2299 2426 551
Size: 1748 127 2435
Fragments arranged by size:
2435 1748 127
MspA1I CmG'CkG
Cuts at: 1421 1735 2095 3108 3353 1421
Size: 314 360 1013 245 2378
Fragments arranged by size:
2378 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 825 909 825
Size: 84 4226
NdeI CA'TA_TG
Cuts at: 258 258
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NsiI A_TGCA'T
Cuts at: 886 886
Size: 4310
NspI r_CATG'y
Cuts at: 167 716 1363 1863 2770 167
Size: 549 647 500 907 1707
Fragments arranged by size:
1707 907 647 549 500
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 3449 3481 214
Size: 32 3203 32 1043
Fragments arranged by size:
3203 1043 32 32
PsiI TTA'TAA
Cuts at: 592 592
Size: 4310
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 4310
SfcI C'TryA_G
Cuts at: 33 137 233 1161 1353 2038 3031 3222 33
Size: 104 96 928 192 685 993 191 1121
Fragments arranged by size:
1121 993 928 685 192 191 104 96
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1231 1465 2380 2872 3134 1231
Size: 234 915 492 262 2407
Fragments arranged by size:
2407 915 492 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 167 1363 1863 167
Size: 1196 500 2614
Fragments arranged by size:
2614 1196 500
StuI AGG'CCT
Cuts at: 901 1069 901
Size: 168 4142
StyI C'CwwG_G
Cuts at: 825 909 1444 1798 825
Size: 84 535 354 3337
Fragments arranged by size:
3337 535 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1893 2026 2485 2694 1893
Size: 133 459 209 3509
Fragments arranged by size:
3509 459 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1805 1805
Size: 4310
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
XmnI GAAnn'nnTTC
Cuts at: 1153 1153
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI