Restriction map for
pF1KB0943
(Circular) (Six-base) MAPSORT of: pF1KB0943.seq Check: 6818 from: 1 to: 3623
pF1KB0943 3623 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 661 661
Size: 3623
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1448 1880 2627 2895 311
Size: 1137 432 747 268 1039
Fragments arranged by size:
1137 1039 747 432 268
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 121 313 928 2495 121
Size: 192 615 1567 1249
Fragments arranged by size:
1567 1249 615 192
ApaI G_GGCC'C
Cuts at: 527 527
Size: 3623
ApaLI G'TGCA_C
Cuts at: 241 2393 241
Size: 2152 1471
ApoI r'AATT_y
Cuts at: 627 2976 3232 627
Size: 2349 256 1018
Fragments arranged by size:
2349 1018 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 643 643
Size: 3623
AvrII C'CTAG_G
Cuts at: 159 159
Size: 3623
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 639 1053 1301 1336 639
Size: 414 248 35 2926
Fragments arranged by size:
2926 414 248 35
BanII G_rGCy'C
Cuts at: 376 527 637 1667 376
Size: 151 110 1030 2332
Fragments arranged by size:
2332 1030 151 110
BbeI G_GCGC'C
Cuts at: 1057 1305 1057
Size: 248 3375
BbsI GAAGACnn'nnnn_
Cuts at: 299 299
Size: 3623
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglI GCCn_nnn'nGGC
Cuts at: 362 371 362
Size: 9 3614
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 735 735
Size: 3623
Bme1580I G_kGCm'C
Cuts at: 245 527 1248 1341 2397 245
Size: 282 721 93 1056 1471
Fragments arranged by size:
1471 1056 721 282 93
BmrI ACTGGGnnnn_n'
Cuts at: 835 916 1251 3395 835
Size: 81 335 2144 1063
Fragments arranged by size:
2144 1063 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 3623
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 1054 1302 2004 3500 1054
Size: 248 702 1496 1177
Fragments arranged by size:
1496 1177 702 248
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2285 2432 2843 812
Size: 521 678 274 147 411 1592
Fragments arranged by size:
1592 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 379 528 1048 1806 2383 2879 379
Size: 149 520 758 577 496 1123
Fragments arranged by size:
1123 758 577 520 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 3623
BsiEI CG_ry'CG
Cuts at: 104 1165 1211 2419 104
Size: 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 46
BsiHKAI G_wGCw'C
Cuts at: 245 321 405 485 637 1415 1605 2397 245
Size: 76 84 80 152 778 190 792 1471
Fragments arranged by size:
1471 792 778 190 152 84 80 76
Bsp1286I G_dGCh'C
Cuts at: 245 321 376 405 485 527 637 1248 1341
Size: 76 55 29 80 42 110 611 93
Cuts at: 1341 1415 1605 1667 2397 245
Size: 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 190 110 93 80 76
74 62 55 42 29
BspEI T'CCGG_A
Cuts at: 2843 2843
Size: 3623
BspHI T'CATG_A
Cuts at: 128 258 2799 128
Size: 130 2541 952
Fragments arranged by size:
2541 952 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 673 887 1189 1570 673
Size: 214 302 381 2726
Fragments arranged by size:
2726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1915 1969 3453 3618 50
Size: 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 1535
Size: 3623
BsrFI r'CCGG_y
Cuts at: 1621 1802 2011 1621
Size: 181 209 3233
Fragments arranged by size:
3233 209 181
BssSI C'ACGA_G
Cuts at: 407 1894 2252 407
Size: 1487 358 1778
Fragments arranged by size:
1778 1487 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 212 1984 212
Size: 1772 1851
BstYI r'GATC_y
Cuts at: 1 603 648 1115 1138 1473 1719 2731 2817
Size: 602 45 467 23 335 246 1012 86
Cuts at: 2817 2829 3175 1
Size: 12 346 449
Fragments arranged by size:
1012 602 467 449 346 335 246 86
45 23 12
BtgI C'CryG_G
Cuts at: 200 200
Size: 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 608 866 608
Size: 258 3365
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 234 325 363 1208 1382 1773 1800 3145 234
Size: 91 38 845 174 391 27 1345 712
Fragments arranged by size:
1345 845 712 391 174 91 38 27
EagI C'GGCC_G
Cuts at: 1208 1208
Size: 3623
EarI CTCTTCn'nnn_
Cuts at: 193 1646 1856 193
Size: 1453 210 1960
Fragments arranged by size:
1960 1453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2141 2287 3433 1831
Size: 310 146 1146 2021
Fragments arranged by size:
2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 635 635
Size: 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 457 1448 1880 2627 2895 311
Size: 146 991 432 747 268 1039
Fragments arranged by size:
1039 991 747 432 268 146
EcoNI CCTnn'n_nnAGG
Cuts at: 495 495
Size: 3623
EcoO109I rG'GnC_Cy
Cuts at: 272 524 762 272
Size: 252 238 3133
Fragments arranged by size:
3133 252 238
EcoRI G'AATT_C
Cuts at: 627 627
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 353 1642 353
Size: 1289 2334
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 1057 1305 2327 3306 1057
Size: 248 1022 979 1374
Fragments arranged by size:
1374 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1465 1495 1497 1527 1465
Size: 30 2 30 3561
Fragments arranged by size:
3561 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
KasI G'GCGC_C
Cuts at: 1053 1301 1053
Size: 248 3375
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 327 365 1384 327
Size: 38 1019 2566
Fragments arranged by size:
2566 1019 38
MslI CAynn'nnrTG
Cuts at: 236 1612 1739 236
Size: 1376 127 2120
Fragments arranged by size:
2120 1376 127
MspA1I CmG'CkG
Cuts at: 734 1048 1408 2421 2666 734
Size: 314 360 1013 245 1691
Fragments arranged by size:
1691 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 1054 1302 1054
Size: 248 3375
NcoI C'CATG_G
Cuts at: 200 200
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NspI r_CATG'y
Cuts at: 676 1176 2083 676
Size: 500 907 2216
Fragments arranged by size:
2216 907 500
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PflMI CCAn_nnn'nTGG
Cuts at: 340 589 340
Size: 249 3374
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2762 2794 103
Size: 32 2627 32 932
Fragments arranged by size:
2627 932 32 32
PpuMI rG'GwC_Cy
Cuts at: 272 272
Size: 3623
PspOMI G'GGCC_C
Cuts at: 523 523
Size: 3623
PstI C_TGCA'G
Cuts at: 670 1355 670
Size: 685 2938
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 1048 1408 1048
Size: 360 3263
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 637 637
Size: 3623
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SexAI A'CCwGG_T
Cuts at: 312 312
Size: 3623
SfcI C'TryA_G
Cuts at: 33 122 666 1351 2344 2535 33
Size: 89 544 685 993 191 1121
Fragments arranged by size:
1121 993 685 544 191 89
SfoI GGC'GCC
Cuts at: 1055 1303 1055
Size: 248 3375
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 778 1693 2185 2447 778
Size: 915 492 262 1954
Fragments arranged by size:
1954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 159 200 328 496 757 1111 159
Size: 41 128 168 261 354 2671
Fragments arranged by size:
2671 354 261 168 128 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 282 1206 1339 1798 2007 282
Size: 924 133 459 209 1898
Fragments arranged by size:
1898 924 459 209 133
TatI w'GTAC_w
Cuts at: 3138 3138
Size: 3623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1118 1118
Size: 3623
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI