Restriction map for
pF1KB0937
(Circular) (Six-base) MAPSORT of: pF1KB0937.seq Check: 3971 from: 1 to: 3593
pF1KB0937 3593 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1850 2597 2865 1418
Size: 432 747 268 2146
Fragments arranged by size:
2146 747 432 268
AfeI AGC'GCT
Cuts at: 190 190
Size: 3593
AflIII A'CryG_T
Cuts at: 945 2049 3026 945
Size: 1104 977 1512
Fragments arranged by size:
1512 1104 977
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 468 898 2465 468
Size: 430 1567 1596
Fragments arranged by size:
1596 1567 430
ApaLI G'TGCA_C
Cuts at: 2363 2363
Size: 3593
ApoI r'AATT_y
Cuts at: 597 2946 3202 597
Size: 2349 256 988
Fragments arranged by size:
2349 988 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 172 246 613 172
Size: 74 367 3152
Fragments arranged by size:
3152 367 74
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 154 609 1023 1271 1306 154
Size: 455 414 248 35 2441
Fragments arranged by size:
2441 455 414 248 35
BanII G_rGCy'C
Cuts at: 121 173 607 1637 121
Size: 52 434 1030 2077
Fragments arranged by size:
2077 1030 434 52
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BbvCI CC'TCA_GC
Cuts at: 529 529
Size: 3593
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglI GCCn_nnn'nGGC
Cuts at: 367 367
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 409 705 409
Size: 296 3297
Bme1580I G_kGCm'C
Cuts at: 157 1218 1311 2367 157
Size: 1061 93 1056 1383
Fragments arranged by size:
1383 1061 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 339 805 886 1221 3365 339
Size: 466 81 335 2144 567
Fragments arranged by size:
2144 567 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 1960 135
Size: 1825 1768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 105
Size: 3593
Bpu10I CC'TnA_GC
Cuts at: 529 529
Size: 3593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 769 1648 2140 2438 412
Size: 357 879 492 298 1567
Fragments arranged by size:
1567 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 3593
BsaAI yAC'GTr
Cuts at: 513 946 513
Size: 433 3160
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2255 2402 2813 782
Size: 521 678 274 147 411 1562
Fragments arranged by size:
1562 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 550 556 580 526
Size: 24 6 24 3539
Fragments arranged by size:
3539 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 178 181 307 127
Size: 51 3 126 3413
Fragments arranged by size:
3413 126 51 3
BseYI C'CCAG_C
Cuts at: 274 1018 1776 2353 2849 274
Size: 744 758 577 496 1018
Fragments arranged by size:
1018 758 744 577 496
BsiEI CG_ry'CG
Cuts at: 104 198 1135 1181 2389 104
Size: 94 937 46 1208 1308
Fragments arranged by size:
1308 1208 937 94 46
BsiHKAI G_wGCw'C
Cuts at: 173 607 1385 1575 2367 173
Size: 434 778 190 792 1399
Fragments arranged by size:
1399 792 778 434 190
Bsp1286I G_dGCh'C
Cuts at: 121 157 173 607 1218 1311 1385 1575 1637
Size: 36 16 434 611 93 74 190 62
Cuts at: 1637 2367 121
Size: 730 1347
Fragments arranged by size:
1347 730 611 434 190 93 74 62
36 16
BspEI T'CCGG_A
Cuts at: 2813 2813
Size: 3593
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 643 857 1159 1540 643
Size: 214 302 381 2696
Fragments arranged by size:
2696 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1885 1939 3423 3588 50
Size: 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1505 1505
Size: 3593
BsrFI r'CCGG_y
Cuts at: 1591 1772 1981 1591
Size: 181 209 3203
Fragments arranged by size:
3203 209 181
BssHII G'CGCG_C
Cuts at: 278 278
Size: 3593
BssSI C'ACGA_G
Cuts at: 520 1864 2222 520
Size: 1344 358 1891
Fragments arranged by size:
1891 1344 358
BstAPI GCAn_nnn'nTGC
Cuts at: 970 970
Size: 3593
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstYI r'GATC_y
Cuts at: 1 416 618 1085 1108 1443 1689 2701 2787
Size: 415 202 467 23 335 246 1012 86
Cuts at: 2787 2799 3145 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 415 346 335 246 202
86 23 12
BtgI C'CryG_G
Cuts at: 107 140 107
Size: 33 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2157 1299
Size: 858 2735
EaeI y'GGCC_r
Cuts at: 195 317 434 1178 1352 1743 1770 3115 195
Size: 122 117 744 174 391 27 1345 673
Fragments arranged by size:
1345 744 673 391 174 122 117 27
EagI C'GGCC_G
Cuts at: 195 1178 195
Size: 983 2610
EarI CTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 567 1801 2111 2257 3403 567
Size: 1234 310 146 1146 757
Fragments arranged by size:
1234 1146 757 310 146
EcoICRI GAG'CTC
Cuts at: 171 605 171
Size: 434 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 1418 1850 2597 2865 105
Size: 1313 432 747 268 833
Fragments arranged by size:
1313 833 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3593
EcoO109I rG'GnC_Cy
Cuts at: 465 732 465
Size: 267 3326
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 376 1374 376
Size: 998 2595
HaeII r_GCGC'y
Cuts at: 192 210 336 373 528 1027 1275 2297 3276
Size: 18 126 37 155 499 248 1022 979
Cuts at: 3276 192
Size: 509
Fragments arranged by size:
1022 979 509 499 248 155 126 37
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1465 1467 1497 1435
Size: 30 2 30 3531
Fragments arranged by size:
3531 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 319 1354 319
Size: 1035 2558
MslI CAynn'nnrTG
Cuts at: 1582 1709 1582
Size: 127 3466
MspA1I CmG'CkG
Cuts at: 503 704 1018 1378 2391 2636 503
Size: 201 314 360 1013 245 1460
Fragments arranged by size:
1460 1013 360 314 245 201
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NcoI C'CATG_G
Cuts at: 107 107
Size: 3593
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2053 646
Size: 500 907 2186
Fragments arranged by size:
2186 907 500
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 3593
PstI C_TGCA'G
Cuts at: 302 473 508 640 1325 302
Size: 171 35 132 685 2570
Fragments arranged by size:
2570 685 171 132 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 503 1018 1378 503
Size: 515 360 2718
Fragments arranged by size:
2718 515 360
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 173 607 173
Size: 434 3159
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SexAI A'CCwGG_T
Cuts at: 461 461
Size: 3593
SfcI C'TryA_G
Cuts at: 33 210 298 469 504 636 1321 2314 2505
Size: 177 88 171 35 132 685 993 191
Cuts at: 2505 33
Size: 1121
Fragments arranged by size:
1121 993 685 191 177 171 132 88
35
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 248 615 248
Size: 367 3226
SmlI C'TyrA_G
Cuts at: 172 427 748 1663 2155 2417 172
Size: 255 321 915 492 262 1348
Fragments arranged by size:
1348 915 492 321 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StuI AGG'CCT
Cuts at: 361 361
Size: 3593
StyI C'CwwG_G
Cuts at: 107 727 1081 107
Size: 620 354 2619
Fragments arranged by size:
2619 620 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 1176 1309 1768 1977 358
Size: 818 133 459 209 1974
Fragments arranged by size:
1974 818 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 550 582 3110 3142 550
Size: 32 2528 32 1001
Fragments arranged by size:
2528 1001 32 32
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 1088
Size: 3593
XhoI C'TCGA_G
Cuts at: 172 172
Size: 3593
XmaI C'CCGG_G
Cuts at: 246 613 246
Size: 367 3226
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI