Restriction map for
pF1KB0936
(Circular) (Six-base) MAPSORT of: pF1KB0936.seq Check: 6696 from: 1 to: 4271
pF1KB0936 4271 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 896 2096 2528 3275 3543 319
Size: 577 1200 432 747 268 1047
Fragments arranged by size:
1200 1047 747 577 432 268
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AleI CACnn'nnGTG
Cuts at: 877 1005 877
Size: 128 4143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 690
Size: 32 4239
AlwNI CAG_nnn'CTG
Cuts at: 342 843 1129 1576 3143 342
Size: 501 286 447 1567 1470
Fragments arranged by size:
1567 1470 501 447 286
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 428 1015 1275 3624 3880 428
Size: 587 260 2349 256 819
Fragments arranged by size:
2349 819 587 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 1291 1291
Size: 4271
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1287 1701 1949 1984 1287
Size: 414 248 35 3574
Fragments arranged by size:
3574 414 248 35
BanII G_rGCy'C
Cuts at: 518 673 1285 2315 518
Size: 155 612 1030 2474
Fragments arranged by size:
2474 1030 612 155
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 1896 1989 3045 1896
Size: 93 1056 3122
Fragments arranged by size:
3122 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 770 1117 1483 1564 1899 4043 667
Size: 103 347 366 81 335 2144 895
Fragments arranged by size:
2144 895 366 347 335 103 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 506 2638 312
Size: 194 2132 1945
Fragments arranged by size:
2132 1945 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 4271
Bpu10I CC'TnA_GC
Cuts at: 1184 1184
Size: 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1224 1447 2326 2818 3116 687
Size: 537 223 879 492 298 1842
Fragments arranged by size:
1842 879 537 492 298 223
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 145 1460 1981 2659 2933 3080 3491 145
Size: 1315 521 678 274 147 411 925
Fragments arranged by size:
1315 925 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 115
Size: 30 4241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 428 932 428
Size: 504 3767
BseYI C'CCAG_C
Cuts at: 1696 2454 3031 3527 1696
Size: 758 577 496 2440
Fragments arranged by size:
2440 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1813 1859 3067 104
Size: 1709 46 1208 1308
Fragments arranged by size:
1709 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 518 1285 2063 2253 3045 518
Size: 767 778 190 792 1744
Fragments arranged by size:
1744 792 778 767 190
BsmI GAATG_Cn'
Cuts at: 986 986
Size: 4271
Bsp1286I G_dGCh'C
Cuts at: 518 673 1285 1896 1989 2063 2253 2315 3045
Size: 155 612 611 93 74 190 62 730
Cuts at: 3045 518
Size: 1744
Fragments arranged by size:
1744 730 612 611 190 155 93 74
62
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 1321 1535 1837 2218 828
Size: 493 214 302 381 2881
Fragments arranged by size:
2881 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 346 2183 346
Size: 1837 2434
BsrFI r'CCGG_y
Cuts at: 495 2269 2450 2659 495
Size: 1774 181 209 2107
Fragments arranged by size:
2107 1774 209 181
BsrGI T'GTAC_A
Cuts at: 1139 1139
Size: 4271
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 1648 1648
Size: 4271
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 246 246
Size: 4271
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 3379 3465 3477
Size: 1295 467 23 335 246 1012 86 12
Cuts at: 3477 3823 1
Size: 346 449
Fragments arranged by size:
1295 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1043 1043
Size: 4271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 386 1514 386
Size: 1128 3143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 149 184 149
Size: 35 4236
DraI TTT'AAA
Cuts at: 221 1270 221
Size: 1049 3222
DrdI GACnn_nn'nnGTC
Cuts at: 199 1977 2835 199
Size: 1778 858 1635
Fragments arranged by size:
1778 1635 858
EaeI y'GGCC_r
Cuts at: 1856 2030 2421 2448 3793 1856
Size: 174 391 27 1345 2334
Fragments arranged by size:
2334 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 338 506 1227 1232 2294 2504 338
Size: 168 721 5 1062 210 2105
Fragments arranged by size:
2105 1062 721 210 168 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2479 2789 2935 4081 2479
Size: 310 146 1146 2669
Fragments arranged by size:
2669 1146 310 146
EcoICRI GAG'CTC
Cuts at: 516 1283 516
Size: 767 3504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 896 1087 2096 2528 3275 3543 319
Size: 577 191 1009 432 747 268 1047
Fragments arranged by size:
1047 1009 747 577 432 268 191
EcoO109I rG'GnC_Cy
Cuts at: 561 679 840 1410 561
Size: 118 161 570 3422
Fragments arranged by size:
3422 570 161 118
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
EcoRV GAT'ATC
Cuts at: 1221 1221
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 2975 3954 1705
Size: 248 1022 979 2022
Fragments arranged by size:
2022 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2113 2143 2145 2175 2113
Size: 30 2 30 4209
Fragments arranged by size:
4209 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MfeI C'AATT_G
Cuts at: 1253 1253
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2942 3126 1189
Size: 1753 184 2334
Fragments arranged by size:
2334 1753 184
MscI TGG'CCA
Cuts at: 2032 2032
Size: 4271
MslI CAynn'nnrTG
Cuts at: 877 1005 2260 2387 877
Size: 128 1255 127 2761
Fragments arranged by size:
2761 1255 128 127
MspA1I CmG'CkG
Cuts at: 1382 1696 2056 3069 3314 1382
Size: 314 360 1013 245 2339
Fragments arranged by size:
2339 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2452 2452
Size: 4271
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 1043 1043
Size: 4271
NdeI CA'TA_TG
Cuts at: 275 275
Size: 4271
NgoMIV G'CCGG_C
Cuts at: 2450 2450
Size: 4271
NspI r_CATG'y
Cuts at: 1324 1824 2731 1324
Size: 500 907 2864
Fragments arranged by size:
2864 907 500
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 952 952
Size: 4271
PfoI T'CCnGG_A
Cuts at: 966 966
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 561 679 840 561
Size: 118 161 3992
Fragments arranged by size:
3992 161 118
PstI C_TGCA'G
Cuts at: 1318 2003 1318
Size: 685 3586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 1696 2056 1696
Size: 360 3911
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 518 1285 518
Size: 767 3504
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SanDI GG'GwC_CC
Cuts at: 561 561
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 506 2294 2504 506
Size: 1788 210 2273
Fragments arranged by size:
2273 1788 210
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 1314 1999 2992 3183 33
Size: 1281 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 666 1203 1426 2341 2833 3095 666
Size: 537 223 915 492 262 1842
Fragments arranged by size:
1842 915 537 492 262 223
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
StyI C'CwwG_G
Cuts at: 1043 1405 1759 1043
Size: 362 354 3555
Fragments arranged by size:
3555 362 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 818 1854 1987 2446 2655 818
Size: 1036 133 459 209 2434
Fragments arranged by size:
2434 1036 459 209 133
TatI w'GTAC_w
Cuts at: 660 1139 3786 660
Size: 479 2647 1145
Fragments arranged by size:
2647 1145 479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 716 1766 716
Size: 1050 3221
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI