Restriction map for
pF1KB0930
(Circular) (Six-base) MAPSORT of: pF1KB0930.seq Check: 7099 from: 1 to: 4226
pF1KB0930 4226 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 213 462 856 1083 2051 2483 3230 3498
Size: 93 249 394 227 968 432 747 268
Cuts at: 3498 120
Size: 848
Fragments arranged by size:
968 848 747 432 394 268 249 227
93
AflIII A'CryG_T
Cuts at: 1578 2682 3659 1578
Size: 1104 977 2145
Fragments arranged by size:
2145 1104 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 952 984 952
Size: 32 4194
AlwNI CAG_nnn'CTG
Cuts at: 1531 3098 1531
Size: 1567 2659
ApaI G_GGCC'C
Cuts at: 201 201
Size: 4226
ApaLI G'TGCA_C
Cuts at: 697 949 2996 697
Size: 252 2047 1927
Fragments arranged by size:
2047 1927 252
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 727 1246 727
Size: 519 3707
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 1242 1656 1904 1939 1242
Size: 414 248 35 3529
Fragments arranged by size:
3529 414 248 35
BanII G_rGCy'C
Cuts at: 201 417 1240 2270 201
Size: 216 823 1030 2157
Fragments arranged by size:
2157 1030 823 216
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BbsI GAAGACnn'nnnn_
Cuts at: 320 320
Size: 4226
BbvCI CC'TCA_GC
Cuts at: 1015 1015
Size: 4226
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 399 1746 399
Size: 1347 2879
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 661 851 1338 661
Size: 190 487 3549
Fragments arranged by size:
3549 487 190
Bme1580I G_kGCm'C
Cuts at: 201 701 727 953 1851 1944 3000 201
Size: 500 26 226 898 93 1056 1427
Fragments arranged by size:
1427 1056 898 500 226 93 26
BmgBI CAC'GTC
Cuts at: 144 213 144
Size: 69 4157
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1519 1854 3998 1438
Size: 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 503
Size: 4226
Bpu10I CC'TnA_GC
Cuts at: 1015 1015
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 1402 2281 2773 3071 144
Size: 1258 879 492 298 1299
Fragments arranged by size:
1299 1258 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 318 3930 318
Size: 3612 614
BsaAI yAC'GTr
Cuts at: 432 647 1579 432
Size: 215 932 3079
Fragments arranged by size:
3079 932 215
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 533 1657 1905 2607 4103 533
Size: 1124 248 702 1496 656
Fragments arranged by size:
1496 1124 702 656 248
BsaWI w'CCGG_w
Cuts at: 746 998 1082 1109 1415 1936 2614 2888 3035
Size: 252 84 27 306 521 678 274 147
Cuts at: 3035 3446 746
Size: 411 1526
Fragments arranged by size:
1526 678 521 411 306 274 252 147
84 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 289 319 952 982 160
Size: 30 99 30 633 30 3404
Fragments arranged by size:
3404 633 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 313 181
Size: 132 4094
BseYI C'CCAG_C
Cuts at: 187 1651 2409 2986 3482 187
Size: 1464 758 577 496 931
Fragments arranged by size:
1464 931 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1102 364
Size: 738 3488
BsiEI CG_ry'CG
Cuts at: 104 589 967 1768 1814 3022 104
Size: 485 378 801 46 1208 1308
Fragments arranged by size:
1308 1208 801 485 378 46
BsiHKAI G_wGCw'C
Cuts at: 566 701 878 953 1240 2018 2208 3000 566
Size: 135 177 75 287 778 190 792 1792
Fragments arranged by size:
1792 792 778 287 190 177 135 75
BsmBI CGTCTCn'nnnn_
Cuts at: 282 282
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 201 417 566 701 727 878 953 1240 1851
Size: 216 149 135 26 151 75 287 611
Cuts at: 1851 1944 2018 2208 2270 3000 201
Size: 93 74 190 62 730 1427
Fragments arranged by size:
1427 730 611 287 216 190 151 149
135 93 75 74 62 26
BspEI T'CCGG_A
Cuts at: 998 3446 998
Size: 2448 1778
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 770 1276 1490 1792 2173 770
Size: 506 214 302 381 2823
Fragments arranged by size:
2823 506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 963 2518 2572 4056 4221 50
Size: 913 1555 54 1484 165 55
Fragments arranged by size:
1555 1484 913 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 674 2224 2405 2614 674
Size: 1550 181 209 2286
Fragments arranged by size:
2286 1550 209 181
BssHII G'CGCG_C
Cuts at: 615 615
Size: 4226
BssSI C'ACGA_G
Cuts at: 888 2497 2855 888
Size: 1609 358 2259
Fragments arranged by size:
2259 1609 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4226
BstYI r'GATC_y
Cuts at: 1 1251 1718 1741 2076 2322 3334 3420 3432
Size: 1250 467 23 335 246 1012 86 12
Cuts at: 3432 3778 1
Size: 346 449
Fragments arranged by size:
1250 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 304 542 107
Size: 197 238 3791
Fragments arranged by size:
3791 238 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 240 347 1469 240
Size: 107 1122 2997
Fragments arranged by size:
2997 1122 107
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 146 358 586 735 964 1146 1811 1985 2376
Size: 212 228 149 229 182 665 174 391
Cuts at: 2376 2403 3748 146
Size: 27 1345 624
Fragments arranged by size:
1345 665 624 391 229 228 212 182
174 149 27
EagI C'GGCC_G
Cuts at: 586 964 1811 586
Size: 378 847 3001
Fragments arranged by size:
3001 847 378
EarI CTCTTCn'nnn_
Cuts at: 1123 2249 2459 1123
Size: 1126 210 2890
Fragments arranged by size:
2890 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 213 462 503 856 1083 2051 2483 3230
Size: 93 249 41 353 227 968 432 747
Cuts at: 3230 3498 120
Size: 268 848
Fragments arranged by size:
968 848 747 432 353 268 249 227
93 41
EcoO109I rG'GnC_Cy
Cuts at: 197 198 352 881 1365 197
Size: 1 154 529 484 3058
Fragments arranged by size:
3058 529 484 154 1
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 606 661 771 1660 1908 2930 3909 606
Size: 55 110 889 248 1022 979 923
Fragments arranged by size:
1022 979 923 889 248 110 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 262 289 294 2068 2098 2100 2130 257
Size: 5 27 5 1774 30 2 30 2353
Fragments arranged by size:
2353 1774 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 950 2897 3081 950
Size: 1947 184 2095
Fragments arranged by size:
2095 1947 184
MscI TGG'CCA
Cuts at: 148 1987 148
Size: 1839 2387
MslI CAynn'nnrTG
Cuts at: 112 2215 2342 112
Size: 2103 127 1996
Fragments arranged by size:
2103 1996 127
MspA1I CmG'CkG
Cuts at: 665 945 1040 1337 1651 2011 3024 3269 665
Size: 280 95 297 314 360 1013 245 1622
Fragments arranged by size:
1622 1013 360 314 297 280 245 95
NaeI GCC'GGC
Cuts at: 676 2407 676
Size: 1731 2495
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 674 2405 674
Size: 1731 2495
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PmlI CAC'GTG
Cuts at: 432 647 432
Size: 215 4011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 616 648 952 984 3365 3397 616
Size: 32 304 32 2381 32 1445
Fragments arranged by size:
2381 1445 304 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 352 352
Size: 4226
PspOMI G'GGCC_C
Cuts at: 197 197
Size: 4226
PstI C_TGCA'G
Cuts at: 381 767 1273 1958 381
Size: 386 506 685 2649
Fragments arranged by size:
2649 685 506 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 665 945 1651 2011 665
Size: 280 706 360 2880
Fragments arranged by size:
2880 706 360 280
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 352 352
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 377 763 1269 1954 2947 3138 33
Size: 344 386 506 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 386 344 191
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 159 1381 2296 2788 3050 159
Size: 1222 915 492 262 1335
Fragments arranged by size:
1335 1222 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StuI AGG'CCT
Cuts at: 715 814 715
Size: 99 4127
StyI C'CwwG_G
Cuts at: 107 710 1004 1091 1360 1714 107
Size: 603 294 87 269 354 2619
Fragments arranged by size:
2619 603 354 294 269 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 3741 3741
Size: 4226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 289 321 3743 3775 160
Size: 32 97 32 3422 32 611
Fragments arranged by size:
3422 611 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 274 2023 274
Size: 1749 2477
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
XmnI GAAnn'nnTTC
Cuts at: 475 799 910 1132 1162 475
Size: 324 111 222 30 3539
Fragments arranged by size:
3539 324 222 111 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI