Restriction map for
pF1KB0927
(Circular) (Six-base) MAPSORT of: pF1KB0927.seq Check: 3332 from: 1 to: 3512
pF1KB0927 3512 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 528 528
Size: 3512
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1769 2516 2784 1337
Size: 432 747 268 2065
Fragments arranged by size:
2065 747 432 268
AflIII A'CryG_T
Cuts at: 447 864 1968 2945 447
Size: 417 1104 977 1014
Fragments arranged by size:
1104 1014 977 417
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 817 2384 817
Size: 1567 1945
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 516 2865 3121 516
Size: 2349 256 907
Fragments arranged by size:
2349 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 532 532
Size: 3512
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 211 528 942 1190 1225 211
Size: 317 414 248 35 2498
Fragments arranged by size:
2498 414 317 248 35
BanII G_rGCy'C
Cuts at: 341 474 526 1556 341
Size: 133 52 1030 2297
Fragments arranged by size:
2297 1030 133 52
BbeI G_GCGC'C
Cuts at: 215 946 1194 215
Size: 731 248 2533
Fragments arranged by size:
2533 731 248
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 1032 1032
Size: 3512
BglII A'GATC_T
Cuts at: 137 1027 3064 137
Size: 890 2037 585
Fragments arranged by size:
2037 890 585
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 1137 1230 2286 1137
Size: 93 1056 2363
Fragments arranged by size:
2363 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 430 724 805 1140 3284 430
Size: 294 81 335 2144 658
Fragments arranged by size:
2144 658 335 294 81
BmtI G_CTAG'C
Cuts at: 491 491
Size: 3512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 419 205
Size: 214 3298
Bpu10I CC'TnA_GC
Cuts at: 294 294
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1567 2059 2357 688
Size: 879 492 298 1843
Fragments arranged by size:
1843 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 320 374 3216 320
Size: 54 2842 616
Fragments arranged by size:
2842 616 54
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 212 307 943 1191 1893 3389 212
Size: 95 636 248 702 1496 335
Fragments arranged by size:
1496 702 636 335 248 95
BsaWI w'CCGG_w
Cuts at: 347 701 1222 1900 2174 2321 2732 347
Size: 354 521 678 274 147 411 1127
Fragments arranged by size:
1127 678 521 411 354 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 518 518
Size: 3512
BseYI C'CCAG_C
Cuts at: 319 441 937 1695 2272 2768 319
Size: 122 496 758 577 496 1063
Fragments arranged by size:
1063 758 577 496 496 122
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 341 526 1304 1494 2286 341
Size: 185 778 190 792 1567
Fragments arranged by size:
1567 792 778 190 185
Bsp1286I G_dGCh'C
Cuts at: 341 474 526 1137 1230 1304 1494 1556 2286
Size: 133 52 611 93 74 190 62 730
Cuts at: 2286 341
Size: 1567
Fragments arranged by size:
1567 730 611 190 133 93 74 62
52
BspEI T'CCGG_A
Cuts at: 347 2732 347
Size: 2385 1127
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 208 1510 1691 1900 208
Size: 1302 181 209 1820
Fragments arranged by size:
1820 1302 209 181
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstYI r'GATC_y
Cuts at: 1 137 537 1004 1027 1362 1608 2620 2706
Size: 136 400 467 23 335 246 1012 86
Cuts at: 2706 2718 3064 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 400 346 335 246 136
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 179 1462 1643 1806 179
Size: 1283 181 163 1885
Fragments arranged by size:
1885 1283 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 206 284 415 477 1097 1271 1662 1689 3034
Size: 78 131 62 620 174 391 27 1345
Cuts at: 3034 206
Size: 684
Fragments arranged by size:
1345 684 620 391 174 131 78 62
27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 398 1535 1745 398
Size: 1137 210 2165
Fragments arranged by size:
2165 1137 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 339 524 339
Size: 185 3327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 419 1337 1769 2516 2784 205
Size: 214 918 432 747 268 933
Fragments arranged by size:
933 918 747 432 268 214
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 3512
EcoO109I rG'GnC_Cy
Cuts at: 651 651
Size: 3512
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1531 1531
Size: 3512
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 215 946 1194 2216 3195 215
Size: 731 248 1022 979 532
Fragments arranged by size:
1022 979 731 532 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 254 286 342 374 1354 1384 1386 1416 254
Size: 32 56 32 980 30 2 30 2350
Fragments arranged by size:
2350 980 56 32 32 30 30 2
HincII GTy'rAC
Cuts at: 551 551
Size: 3512
HindIII A'AGCT_T
Cuts at: 291 291
Size: 3512
KasI G'GCGC_C
Cuts at: 211 942 1190 211
Size: 731 248 2533
Fragments arranged by size:
2533 731 248
KpnI G_GTAC'C
Cuts at: 532 532
Size: 3512
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 1273 1273
Size: 3512
MslI CAynn'nnrTG
Cuts at: 1501 1628 1501
Size: 127 3385
MspA1I CmG'CkG
Cuts at: 323 623 937 1297 2310 2555 323
Size: 300 314 360 1013 245 1280
Fragments arranged by size:
1280 1013 360 314 300 245
NaeI GCC'GGC
Cuts at: 210 1693 210
Size: 1483 2029
NarI GG'CG_CC
Cuts at: 212 943 1191 212
Size: 731 248 2533
Fragments arranged by size:
2533 731 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3512
NgoMIV G'CCGG_C
Cuts at: 208 1691 208
Size: 1483 2029
NheI G'CTAG_C
Cuts at: 487 487
Size: 3512
NspI r_CATG'y
Cuts at: 451 565 1065 1972 451
Size: 114 500 907 1991
Fragments arranged by size:
1991 907 500 114
PasI CC'CwG_GG
Cuts at: 301 301
Size: 3512
PciI A'CATG_T
Cuts at: 447 1968 447
Size: 1521 1991
PfoI T'CCnGG_A
Cuts at: 221 221
Size: 3512
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PstI C_TGCA'G
Cuts at: 559 1244 559
Size: 685 2827
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 937 1297 937
Size: 360 3152
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 341 526 341
Size: 185 3327
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 398 1535 1745 398
Size: 1137 210 2165
Fragments arranged by size:
2165 1137 210
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 555 1240 2233 2424 33
Size: 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 191
SfoI GGC'GCC
Cuts at: 213 944 1192 213
Size: 731 248 2533
Fragments arranged by size:
2533 731 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 667 1582 2074 2336 667
Size: 915 492 262 1843
Fragments arranged by size:
1843 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
StyI C'CwwG_G
Cuts at: 107 646 1000 107
Size: 539 354 2619
Fragments arranged by size:
2619 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 1228 1687 1896 1095
Size: 133 459 209 2711
Fragments arranged by size:
2711 459 209 133
TatI w'GTAC_w
Cuts at: 3027 3027
Size: 3512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 463 1007 363
Size: 100 544 2868
Fragments arranged by size:
2868 544 100
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI