Restriction map for
pF1KB0923
(Circular) (Six-base) MAPSORT of: pF1KB0923.seq Check: 4101 from: 1 to: 3479
pF1KB0923 3479 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 517 517
Size: 3479
Acc65I G'GTAC_C
Cuts at: 495 495
Size: 3479
AclI AA'CG_TT
Cuts at: 3319 3319
Size: 3479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1736 2483 2751 1304
Size: 432 747 268 2032
Fragments arranged by size:
2032 747 432 268
AflII C'TTAA_G
Cuts at: 154 154
Size: 3479
AflIII A'CryG_T
Cuts at: 831 1935 2912 831
Size: 1104 977 1398
Fragments arranged by size:
1398 1104 977
AgeI A'CCGG_T
Cuts at: 1867 1867
Size: 3479
AlwNI CAG_nnn'CTG
Cuts at: 126 784 2351 126
Size: 658 1567 1254
Fragments arranged by size:
1567 1254 658
ApaLI G'TGCA_C
Cuts at: 2249 2249
Size: 3479
ApoI r'AATT_y
Cuts at: 483 2832 3088 483
Size: 2349 256 874
Fragments arranged by size:
2349 874 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3479
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
AvaI C'yCGr_G
Cuts at: 398 499 398
Size: 101 3378
BamHI G'GATC_C
Cuts at: 504 504
Size: 3479
BanI G'GyrC_C
Cuts at: 495 909 1157 1192 495
Size: 414 248 35 2782
Fragments arranged by size:
2782 414 248 35
BanII G_rGCy'C
Cuts at: 493 1523 493
Size: 1030 2449
BbeI G_GCGC'C
Cuts at: 913 1161 913
Size: 248 3231
BciVI GTATCCnnnnn_n'
Cuts at: 1377 2138 3479 1377
Size: 761 1341 1377
Fragments arranged by size:
1377 1341 761
BclI T'GATC_A
Cuts at: 999 999
Size: 3479
BglII A'GATC_T
Cuts at: 994 3031 994
Size: 2037 1442
BlpI GC'TnA_GC
Cuts at: 591 591
Size: 3479
Bme1580I G_kGCm'C
Cuts at: 1104 1197 2253 1104
Size: 93 1056 2330
Fragments arranged by size:
2330 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 691 772 1107 3251 691
Size: 81 335 2144 919
Fragments arranged by size:
2144 919 335 81
BmtI G_CTAG'C
Cuts at: 230 230
Size: 3479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 148
Size: 3479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1534 2026 2324 655
Size: 879 492 298 1810
Fragments arranged by size:
1810 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3183 3183
Size: 3479
BsaAI yAC'GTr
Cuts at: 832 832
Size: 3479
BsaBI GATnn'nnATC
Cuts at: 6 993 3030 6
Size: 987 2037 455
Fragments arranged by size:
2037 987 455
BsaHI Gr'CG_yC
Cuts at: 910 1158 1860 3356 910
Size: 248 702 1496 1033
Fragments arranged by size:
1496 1033 702 248
BsaWI w'CCGG_w
Cuts at: 668 1189 1867 2141 2288 2699 668
Size: 521 678 274 147 411 1448
Fragments arranged by size:
1448 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 904 1662 2239 2735 904
Size: 758 577 496 1648
Fragments arranged by size:
1648 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1021 1067 2275 104
Size: 917 46 1208 1308
Fragments arranged by size:
1308 1208 917 46
BsiHKAI G_wGCw'C
Cuts at: 493 1271 1461 2253 493
Size: 778 190 792 1719
Fragments arranged by size:
1719 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 493 1104 1197 1271 1461 1523 2253 493
Size: 611 93 74 190 62 730 1719
Fragments arranged by size:
1719 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2699 2699
Size: 3479
BspHI T'CATG_A
Cuts at: 2655 2655
Size: 3479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 529 743 1045 1426 529
Size: 214 302 381 2582
Fragments arranged by size:
2582 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1771 1825 3309 3474 50
Size: 1721 54 1484 165 55
Fragments arranged by size:
1721 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 268 1391 268
Size: 1123 2356
BsrFI r'CCGG_y
Cuts at: 467 1477 1658 1867 467
Size: 1010 181 209 2079
Fragments arranged by size:
2079 1010 209 181
BssSI C'ACGA_G
Cuts at: 1750 2108 1750
Size: 358 3121
BstAPI GCAn_nnn'nTGC
Cuts at: 856 856
Size: 3479
BstBI TT'CG_AA
Cuts at: 1840 1840
Size: 3479
BstYI r'GATC_y
Cuts at: 1 504 971 994 1329 1575 2587 2673 2685
Size: 503 467 23 335 246 1012 86 12
Cuts at: 2685 3031 1
Size: 346 449
Fragments arranged by size:
1012 503 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 200 107
Size: 93 3386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1429 1610 1773 1429
Size: 181 163 3135
Fragments arranged by size:
3135 181 163
BtsI GCAGTG_nn'
Cuts at: 722 722
Size: 3479
DraI TTT'AAA
Cuts at: 478 478
Size: 3479
DrdI GACnn_nn'nnGTC
Cuts at: 1185 2043 1185
Size: 858 2621
EaeI y'GGCC_r
Cuts at: 1064 1238 1629 1656 3001 1064
Size: 174 391 27 1345 1542
Fragments arranged by size:
1542 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1064 1064
Size: 3479
EarI CTCTTCn'nnn_
Cuts at: 107 322 1502 1712 107
Size: 215 1180 210 1874
Fragments arranged by size:
1874 1180 215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 396 1687 1997 2143 3289 396
Size: 1291 310 146 1146 586
Fragments arranged by size:
1291 1146 586 310 146
EcoICRI GAG'CTC
Cuts at: 491 491
Size: 3479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1304 1736 2483 2751 148
Size: 1156 432 747 268 876
Fragments arranged by size:
1156 876 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 196 618 196
Size: 422 3057
EcoRI G'AATT_C
Cuts at: 483 483
Size: 3479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 392 405 1498 169
Size: 223 13 1093 2150
Fragments arranged by size:
2150 1093 223 13
FspI TGC'GCA
Cuts at: 1260 1260
Size: 3479
HaeII r_GCGC'y
Cuts at: 913 1161 2183 3162 913
Size: 248 1022 979 1230
Fragments arranged by size:
1230 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 201 233 1321 1351 1353 1383 201
Size: 32 1088 30 2 30 2297
Fragments arranged by size:
2297 1088 32 30 30 2
HincII GTy'rAC
Cuts at: 518 518
Size: 3479
KasI G'GCGC_C
Cuts at: 909 1157 909
Size: 248 3231
KpnI G_GTAC'C
Cuts at: 499 499
Size: 3479
MluI A'CGCG_T
Cuts at: 2912 2912
Size: 3479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 187 488 2150 2334 187
Size: 301 1662 184 1332
Fragments arranged by size:
1662 1332 301 184
MscI TGG'CCA
Cuts at: 1240 1240
Size: 3479
MslI CAynn'nnrTG
Cuts at: 1468 1595 1468
Size: 127 3352
MspA1I CmG'CkG
Cuts at: 590 904 1264 2277 2522 590
Size: 314 360 1013 245 1547
Fragments arranged by size:
1547 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1660 1660
Size: 3479
NarI GG'CG_CC
Cuts at: 910 1158 910
Size: 248 3231
NcoI C'CATG_G
Cuts at: 107 200 107
Size: 93 3386
NgoMIV G'CCGG_C
Cuts at: 1658 1658
Size: 3479
NheI G'CTAG_C
Cuts at: 226 226
Size: 3479
NspI r_CATG'y
Cuts at: 532 1032 1939 532
Size: 500 907 2072
Fragments arranged by size:
2072 907 500
PciI A'CATG_T
Cuts at: 1935 1935
Size: 3479
PflMI CCAn_nnn'nTGG
Cuts at: 206 206
Size: 3479
PmeI GTTT'AAAC
Cuts at: 478 478
Size: 3479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 3447
PpuMI rG'GwC_Cy
Cuts at: 196 196
Size: 3479
PspXI vC'TCGA_Gb
Cuts at: 398 398
Size: 3479
PstI C_TGCA'G
Cuts at: 526 1211 526
Size: 685 2794
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3479
PvuII CAG'CTG
Cuts at: 904 1264 904
Size: 360 3119
RsrII CG'GwC_CG
Cuts at: 1674 1674
Size: 3479
SacI G_AGCT'C
Cuts at: 493 493
Size: 3479
SalI G'TCGA_C
Cuts at: 516 516
Size: 3479
SanDI GG'GwC_CC
Cuts at: 196 196
Size: 3479
SapI GCTCTTCn'nnn_
Cuts at: 322 1502 1712 322
Size: 1180 210 2089
Fragments arranged by size:
2089 1180 210
SbfI CC_TGCA'GG
Cuts at: 526 526
Size: 3479
ScaI AGT'ACT
Cuts at: 2996 2996
Size: 3479
SfcI C'TryA_G
Cuts at: 33 522 1207 2200 2391 33
Size: 489 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 191
SfoI GGC'GCC
Cuts at: 911 1159 911
Size: 248 3231
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3479
SmaI CCC'GGG
Cuts at: 501 501
Size: 3479
SmlI C'TyrA_G
Cuts at: 154 398 634 1549 2041 2303 154
Size: 244 236 915 492 262 1330
Fragments arranged by size:
1330 915 492 262 244 236
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3479
SphI G_CATG'C
Cuts at: 532 1032 532
Size: 500 2979
StyI C'CwwG_G
Cuts at: 107 200 613 967 107
Size: 93 413 354 2619
Fragments arranged by size:
2619 413 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1062 1195 1654 1863 1062
Size: 133 459 209 2678
Fragments arranged by size:
2678 459 209 133
TatI w'GTAC_w
Cuts at: 2994 2994
Size: 3479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3447
Tth111I GACn'n_nGTC
Cuts at: 1276 1276
Size: 3479
XbaI T'CTAG_A
Cuts at: 510 510
Size: 3479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 974 974
Size: 3479
XhoI C'TCGA_G
Cuts at: 398 398
Size: 3479
XmaI C'CCGG_G
Cuts at: 499 499
Size: 3479
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI