Restriction map for
pF1KB0919
(Circular) (Six-base) MAPSORT of: pF1KB0919.seq Check: 8459 from: 1 to: 3467
pF1KB0919 3467 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1292 1724 2471 2739 237
Size: 1055 432 747 268 965
Fragments arranged by size:
1055 965 747 432 268
AflIII A'CryG_T
Cuts at: 819 1923 2900 819
Size: 1104 977 1386
Fragments arranged by size:
1386 1104 977
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 199 231 199
Size: 32 3435
AlwNI CAG_nnn'CTG
Cuts at: 177 333 772 2339 177
Size: 156 439 1567 1305
Fragments arranged by size:
1567 1305 439 156
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 471 2820 3076 471
Size: 2349 256 862
Fragments arranged by size:
2349 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 279 487 279
Size: 208 3259
BamHI G'GATC_C
Cuts at: 207 492 207
Size: 285 3182
BanI G'GyrC_C
Cuts at: 483 897 1145 1180 483
Size: 414 248 35 2770
Fragments arranged by size:
2770 414 248 35
BanII G_rGCy'C
Cuts at: 115 374 481 1511 115
Size: 259 107 1030 2071
Fragments arranged by size:
2071 1030 259 107
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 3467
BbvCI CC'TCA_GC
Cuts at: 355 355
Size: 3467
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 987 987
Size: 3467
BglI GCCn_nnn'nGGC
Cuts at: 316 316
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 1092 1185 2241 1092
Size: 93 1056 2318
Fragments arranged by size:
2318 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 238 679 760 1095 3239 238
Size: 441 81 335 2144 466
Fragments arranged by size:
2144 466 441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 307 468 1834 307
Size: 161 1366 1940
Fragments arranged by size:
1940 1366 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 418 199
Size: 219 3248
Bpu10I CC'TnA_GC
Cuts at: 355 355
Size: 3467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1522 2014 2312 643
Size: 879 492 298 1798
Fragments arranged by size:
1798 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 3467
BsaAI yAC'GTr
Cuts at: 315 820 315
Size: 505 2962
BsaBI GATnn'nnATC
Cuts at: 6 981 3018 6
Size: 975 2037 455
Fragments arranged by size:
2037 975 455
BsaHI Gr'CG_yC
Cuts at: 898 1146 1848 3344 898
Size: 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 656 1177 1855 2129 2276 2687 656
Size: 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 444 444
Size: 3467
BseYI C'CCAG_C
Cuts at: 150 892 1650 2227 2723 150
Size: 742 758 577 496 894
Fragments arranged by size:
894 758 742 577 496
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 374 481 1259 1449 2241 374
Size: 107 778 190 792 1600
Fragments arranged by size:
1600 792 778 190 107
Bsp1286I G_dGCh'C
Cuts at: 115 374 481 1092 1185 1259 1449 1511 2241
Size: 259 107 611 93 74 190 62 730
Cuts at: 2241 115
Size: 1341
Fragments arranged by size:
1341 730 611 259 190 107 93 74
62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 2643 2643
Size: 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 731 1033 1414 517
Size: 214 302 381 2570
Fragments arranged by size:
2570 381 302 214
BsrBI CCG'CTC
Cuts at: 50 402 1759 1813 3297 3462 50
Size: 352 1357 54 1484 165 55
Fragments arranged by size:
1484 1357 352 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 198 385 435 1465 1646 1855 198
Size: 187 50 1030 181 209 1810
Fragments arranged by size:
1810 1030 209 187 181 50
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstYI r'GATC_y
Cuts at: 1 173 207 492 959 982 1317 1563 2575
Size: 172 34 285 467 23 335 246 1012
Cuts at: 2575 2661 2673 3019 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 285 246 172
86 34 23 12
Bsu36I CC'TnA_GG
Cuts at: 456 456
Size: 3467
BtgI C'CryG_G
Cuts at: 107 161 107
Size: 54 3413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 710 710
Size: 3467
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 1052 1226 1617 1644 2989 1052
Size: 174 391 27 1345 1530
Fragments arranged by size:
1530 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 372 479 372
Size: 107 3360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 237 418 1292 1724 2471 2739 199
Size: 38 181 874 432 747 268 927
Fragments arranged by size:
927 874 747 432 268 181 38
EcoO109I rG'GnC_Cy
Cuts at: 276 393 606 276
Size: 117 213 3137
Fragments arranged by size:
3137 213 117
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 157 901 1149 2171 3150 157
Size: 744 248 1022 979 474
Fragments arranged by size:
1022 979 744 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1309 1339 1341 1371 1309
Size: 30 2 30 3405
Fragments arranged by size:
3405 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 1456 1583 1456
Size: 127 3340
MspA1I CmG'CkG
Cuts at: 330 578 892 1252 2265 2510 330
Size: 248 314 360 1013 245 1287
Fragments arranged by size:
1287 1013 360 314 248 245
NaeI GCC'GGC
Cuts at: 200 387 1648 200
Size: 187 1261 2019
Fragments arranged by size:
2019 1261 187
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NcoI C'CATG_G
Cuts at: 107 107
Size: 3467
NgoMIV G'CCGG_C
Cuts at: 198 385 1646 198
Size: 187 1261 2019
Fragments arranged by size:
2019 1261 187
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PpuMI rG'GwC_Cy
Cuts at: 393 393
Size: 3467
PstI C_TGCA'G
Cuts at: 338 380 514 1199 338
Size: 42 134 685 2606
Fragments arranged by size:
2606 685 134 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 374 481 374
Size: 107 3360
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 334 376 510 1195 2188 2379 33
Size: 301 42 134 685 993 191 1121
Fragments arranged by size:
1121 993 685 301 191 134 42
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 281 489 281
Size: 208 3259
SmlI C'TyrA_G
Cuts at: 622 1537 2029 2291 622
Size: 915 492 262 1798
Fragments arranged by size:
1798 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 107 116 601 955 107
Size: 9 485 354 2619
Fragments arranged by size:
2619 485 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 2982 2982
Size: 3467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3435
Tth111I GACn'n_nGTC
Cuts at: 1264 1264
Size: 3467
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 279 487 279
Size: 208 3259
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI