Restriction map for
pF1KB0918
(Circular) (Six-base) MAPSORT of: pF1KB0918.seq Check: 7040 from: 1 to: 4106
pF1KB0918 4106 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 851 1000 1931 2363 3110 3378 317
Size: 534 149 931 432 747 268 1045
Fragments arranged by size:
1045 931 747 534 432 268 149
AflII C'TTAA_G
Cuts at: 769 769
Size: 4106
AflIII A'CryG_T
Cuts at: 238 1458 2562 3539 238
Size: 1220 1104 977 805
Fragments arranged by size:
1220 1104 977 805
AgeI A'CCGG_T
Cuts at: 199 2494 199
Size: 2295 1811
AlwNI CAG_nnn'CTG
Cuts at: 1411 2978 1411
Size: 1567 2539
ApaLI G'TGCA_C
Cuts at: 242 896 2876 242
Size: 654 1980 1472
Fragments arranged by size:
1980 1472 654
ApoI r'AATT_y
Cuts at: 439 557 616 936 962 1110 3459 3715 439
Size: 118 59 320 26 148 2349 256 830
Fragments arranged by size:
2349 830 320 256 148 118 59 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 133 1126 133
Size: 993 3113
BamHI G'GATC_C
Cuts at: 360 1131 360
Size: 771 3335
BanI G'GyrC_C
Cuts at: 148 1122 1536 1784 1819 148
Size: 974 414 248 35 2435
Fragments arranged by size:
2435 974 414 248 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3076
BbeI G_GCGC'C
Cuts at: 152 1540 1788 152
Size: 1388 248 2470
Fragments arranged by size:
2470 1388 248
BbsI GAAGACnn'nnnn_
Cuts at: 929 929
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BfrBI ATG'CAT
Cuts at: 970 970
Size: 4106
BglI GCCn_nnn'nGGC
Cuts at: 129 129
Size: 4106
BglII A'GATC_T
Cuts at: 1059 1621 3658 1059
Size: 562 2037 1507
Fragments arranged by size:
2037 1507 562
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 246 900 1731 1824 2880 246
Size: 654 831 93 1056 1472
Fragments arranged by size:
1472 1056 831 654 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 359 1620 3657 6
Size: 353 1261 2037 455
Fragments arranged by size:
2037 1261 455 353
BsaHI Gr'CG_yC
Cuts at: 118 149 1537 1785 2487 3983 118
Size: 31 1388 248 702 1496 241
Fragments arranged by size:
1496 1388 702 248 241 31
BsaWI w'CCGG_w
Cuts at: 199 1295 1816 2494 2768 2915 3326 199
Size: 1096 521 678 274 147 411 979
Fragments arranged by size:
1096 979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 449 479 449
Size: 30 4076
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 470 178
Size: 292 3814
BseYI C'CCAG_C
Cuts at: 1531 2289 2866 3362 1531
Size: 758 577 496 2275
Fragments arranged by size:
2275 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 146 246 900 1042 1120 1898 2088 2880 146
Size: 100 654 142 78 778 190 792 1372
Fragments arranged by size:
1372 792 778 654 190 142 100 78
Bsp1286I G_dGCh'C
Cuts at: 146 246 900 1042 1120 1731 1824 1898 2088
Size: 100 654 142 78 611 93 74 190
Cuts at: 2088 2150 2880 146
Size: 62 730 1372
Fragments arranged by size:
1372 730 654 611 190 142 100 93
78 74 62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 309 3282 309
Size: 2973 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 199 2104 2285 2494 199
Size: 1905 181 209 1811
Fragments arranged by size:
1905 1811 209 181
BsrGI T'GTAC_A
Cuts at: 672 672
Size: 4106
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 920 2467 920
Size: 1547 2559
BstYI r'GATC_y
Cuts at: 1 360 1059 1131 1598 1621 1956 2202 3214
Size: 359 699 72 467 23 335 246 1012
Cuts at: 3214 3300 3312 3658 1
Size: 86 12 346 449
Fragments arranged by size:
1012 699 467 449 359 346 335 246
86 72 23 12
BtgI C'CryG_G
Cuts at: 107 464 107
Size: 357 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 585 2056 2237 2400 585
Size: 1471 181 163 2291
Fragments arranged by size:
2291 1471 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 964 999 964
Size: 35 4071
DraI TTT'AAA
Cuts at: 479 1105 479
Size: 626 3480
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1691 1865 2256 2283 3628 1691
Size: 174 391 27 1345 2169
Fragments arranged by size:
2169 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 719 995 2129 2339 719
Size: 276 1134 210 2486
Fragments arranged by size:
2486 1134 276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 851 1000 1931 2363 3110 3378 317
Size: 534 149 931 432 747 268 1045
Fragments arranged by size:
1045 931 747 534 432 268 149
EcoO109I rG'GnC_Cy
Cuts at: 1245 1245
Size: 4106
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 808 2125 808
Size: 1317 2789
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 152 187 1540 1788 2810 3789 152
Size: 35 1353 248 1022 979 469
Fragments arranged by size:
1353 1022 979 469 248 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 588 620 1051 1083 1948 1978 1980
Size: 32 177 32 431 32 865 30 2
Cuts at: 1980 2010 379
Size: 30 2475
Fragments arranged by size:
2475 865 431 177 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
KasI G'GCGC_C
Cuts at: 148 1536 1784 148
Size: 1388 248 2470
Fragments arranged by size:
2470 1388 248
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 743 2095 2222 743
Size: 1352 127 2627
Fragments arranged by size:
2627 1352 127
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 149 1537 1785 149
Size: 1388 248 2470
Fragments arranged by size:
2470 1388 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NsiI A_TGCA'T
Cuts at: 972 972
Size: 4106
NspI r_CATG'y
Cuts at: 242 586 1159 1659 2566 242
Size: 344 573 500 907 1782
Fragments arranged by size:
1782 907 573 500 344
PciI A'CATG_T
Cuts at: 238 2562 238
Size: 2324 1782
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PsiI TTA'TAA
Cuts at: 563 563
Size: 4106
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 719 2129 2339 719
Size: 1410 210 2486
Fragments arranged by size:
2486 1410 210
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 909 1149 1834 2827 3018 33
Size: 876 240 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 240 191
SfoI GGC'GCC
Cuts at: 150 1538 1786 150
Size: 1388 248 2470
Fragments arranged by size:
2470 1388 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SgrAI Cr'CCGG_yG
Cuts at: 199 199
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 769 1261 2176 2668 2930 769
Size: 492 915 492 262 1945
Fragments arranged by size:
1945 915 492 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
SspI AAT'ATT
Cuts at: 818 818
Size: 4106
StyI C'CwwG_G
Cuts at: 107 1240 1594 107
Size: 1133 354 2619
Fragments arranged by size:
2619 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 672 3621 672
Size: 2949 1157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 3623 3655 449
Size: 32 3142 32 900
Fragments arranged by size:
3142 900 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI BpmI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI