Restriction map for
pF1KB0908
(Circular) (Six-base) MAPSORT of: pF1KB0908.seq Check: 5545 from: 1 to: 4046
pF1KB0908 4046 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1039 1039
Size: 4046
AccI GT'mk_AC
Cuts at: 546 770 1084 546
Size: 224 314 3508
Fragments arranged by size:
3508 314 224
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1871 2303 3050 3318 232
Size: 1639 432 747 268 960
Fragments arranged by size:
1639 960 747 432 268
AfeI AGC'GCT
Cuts at: 955 955
Size: 4046
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 482 482
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 177 1351 2918 177
Size: 1174 1567 1305
Fragments arranged by size:
1567 1305 1174
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 1050 3399 3655 1050
Size: 2349 256 1441
Fragments arranged by size:
2349 1441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 390 1071 390
Size: 681 3365
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 1060 2090 1060
Size: 1030 3016
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 183 217 183
Size: 34 4012
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 602 1566 602
Size: 964 3082
BglI GCCn_nnn'nGGC
Cuts at: 172 172
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 557 1158 557
Size: 601 3445
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmgBI CAC'GTC
Cuts at: 117 117
Size: 4046
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 168 262 160
Size: 8 94 3944
Fragments arranged by size:
3944 94 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 401 401
Size: 4046
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 229 229
Size: 4046
Bsp1286I G_dGCh'C
Cuts at: 1060 1671 1764 1838 2028 2090 2820 1060
Size: 611 93 74 190 62 730 2286
Fragments arranged by size:
2286 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 905 3222 905
Size: 2317 1729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1029 1039 1096 1310 1612 1993 1029
Size: 10 57 214 302 381 3082
Fragments arranged by size:
3082 381 302 214 57 10
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 677 1958 677
Size: 1281 2765
BsrFI r'CCGG_y
Cuts at: 1034 2044 2225 2434 1034
Size: 1010 181 209 2646
Fragments arranged by size:
2646 1010 209 181
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstEII G'GTnAC_C
Cuts at: 681 681
Size: 4046
BstYI r'GATC_y
Cuts at: 1 390 1071 1538 1561 1896 2142 3154 3240
Size: 389 681 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 681 467 449 389 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 848 1289 848
Size: 441 3605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 552 587 552
Size: 35 4011
DraI TTT'AAA
Cuts at: 491 1045 491
Size: 554 3492
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 1631 1805 2196 2223 3568 1631
Size: 174 391 27 1345 2109
Fragments arranged by size:
2109 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 368 2069 2279 368
Size: 1701 210 2135
Fragments arranged by size:
2135 1701 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2254 2564 2710 3856 127
Size: 2127 310 146 1146 317
Fragments arranged by size:
2127 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 168 232 262 1871 2303 3050 3318 160
Size: 8 64 30 1609 432 747 268 888
Fragments arranged by size:
1609 888 747 432 268 64 30 8
EcoO109I rG'GnC_Cy
Cuts at: 303 942 1185 303
Size: 639 243 3164
Fragments arranged by size:
3164 639 243
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 269 1827 269
Size: 1558 2488
HaeII r_GCGC'y
Cuts at: 178 957 1480 1728 2750 3729 178
Size: 779 523 248 1022 979 495
Fragments arranged by size:
1022 979 779 523 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 1888 1918 1920 1950 784
Size: 32 1072 30 2 30 2880
Fragments arranged by size:
2880 1072 32 30 30 2
HincII GTy'rAC
Cuts at: 297 424 1085 297
Size: 127 661 3258
Fragments arranged by size:
3258 661 127
HindIII A'AGCT_T
Cuts at: 787 787
Size: 4046
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 2717 2901 833
Size: 1884 184 1978
Fragments arranged by size:
1978 1884 184
MscI TGG'CCA
Cuts at: 1807 1807
Size: 4046
MslI CAynn'nnrTG
Cuts at: 371 441 2035 2162 371
Size: 70 1594 127 2255
Fragments arranged by size:
2255 1594 127 70
MspA1I CmG'CkG
Cuts at: 870 1157 1471 1831 2844 3089 870
Size: 287 314 360 1013 245 1827
Fragments arranged by size:
1827 1013 360 314 287 245
NaeI GCC'GGC
Cuts at: 1036 2227 1036
Size: 1191 2855
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 107
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 1034 2225 1034
Size: 1191 2855
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PfoI T'CCnGG_A
Cuts at: 275 275
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PpuMI rG'GwC_Cy
Cuts at: 303 303
Size: 4046
PstI C_TGCA'G
Cuts at: 974 1093 1778 974
Size: 119 685 3242
Fragments arranged by size:
3242 685 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 870 1471 1831 870
Size: 601 360 3085
Fragments arranged by size:
3085 601 360
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SexAI A'CCwGG_T
Cuts at: 910 910
Size: 4046
SfcI C'TryA_G
Cuts at: 33 417 970 1089 1774 2767 2958 33
Size: 384 553 119 685 993 191 1121
Fragments arranged by size:
1121 993 685 553 384 191 119
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StuI AGG'CCT
Cuts at: 145 259 145
Size: 114 3932
StyI C'CwwG_G
Cuts at: 107 686 1180 1534 107
Size: 579 494 354 2619
Fragments arranged by size:
2619 579 494 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 282 1629 1762 2221 2430 282
Size: 1347 133 459 209 1898
Fragments arranged by size:
1898 1347 459 209 133
TatI w'GTAC_w
Cuts at: 3561 3561
Size: 4046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 1541
Size: 4046
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI