Restriction map for
pF1KB0907
(Circular) (Six-base) MAPSORT of: pF1KB0907.seq Check: 4622 from: 1 to: 3395
pF1KB0907 3395 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 433 433
Size: 3395
Acc65I G'GTAC_C
Cuts at: 254 411 254
Size: 157 3238
AclI AA'CG_TT
Cuts at: 3235 3235
Size: 3395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1652 2399 2667 1220
Size: 432 747 268 1948
Fragments arranged by size:
1948 747 432 268
AflII C'TTAA_G
Cuts at: 270 270
Size: 3395
AflIII A'CryG_T
Cuts at: 747 1851 2828 747
Size: 1104 977 1314
Fragments arranged by size:
1314 1104 977
AgeI A'CCGG_T
Cuts at: 1783 1783
Size: 3395
AlwNI CAG_nnn'CTG
Cuts at: 700 2267 700
Size: 1567 1828
ApaLI G'TGCA_C
Cuts at: 2165 2165
Size: 3395
ApoI r'AATT_y
Cuts at: 399 2748 3004 399
Size: 2349 256 790
Fragments arranged by size:
2349 790 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3395
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3395
AvaI C'yCGr_G
Cuts at: 415 415
Size: 3395
BamHI G'GATC_C
Cuts at: 420 420
Size: 3395
BanI G'GyrC_C
Cuts at: 254 411 825 1073 1108 254
Size: 157 414 248 35 2541
Fragments arranged by size:
2541 414 248 157 35
BanII G_rGCy'C
Cuts at: 334 409 1439 334
Size: 75 1030 2290
Fragments arranged by size:
2290 1030 75
BbeI G_GCGC'C
Cuts at: 829 1077 829
Size: 248 3147
BciVI GTATCCnnnnn_n'
Cuts at: 1293 2054 3395 1293
Size: 761 1341 1293
Fragments arranged by size:
1341 1293 761
BclI T'GATC_A
Cuts at: 915 915
Size: 3395
BglII A'GATC_T
Cuts at: 910 2947 910
Size: 2037 1358
BlpI GC'TnA_GC
Cuts at: 507 507
Size: 3395
Bme1580I G_kGCm'C
Cuts at: 1020 1113 2169 1020
Size: 93 1056 2246
Fragments arranged by size:
2246 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 688 1023 3167 607
Size: 81 335 2144 835
Fragments arranged by size:
2144 835 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 571 1450 1942 2240 192
Size: 379 879 492 298 1347
Fragments arranged by size:
1347 879 492 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 3099 3099
Size: 3395
BsaAI yAC'GTr
Cuts at: 748 748
Size: 3395
BsaBI GATnn'nnATC
Cuts at: 6 909 2946 6
Size: 903 2037 455
Fragments arranged by size:
2037 903 455
BsaHI Gr'CG_yC
Cuts at: 826 1074 1776 3272 826
Size: 248 702 1496 949
Fragments arranged by size:
1496 949 702 248
BsaWI w'CCGG_w
Cuts at: 584 1105 1783 2057 2204 2615 584
Size: 521 678 274 147 411 1364
Fragments arranged by size:
1364 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 820 1578 2155 2651 820
Size: 758 577 496 1564
Fragments arranged by size:
1564 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 937 983 2191 104
Size: 833 46 1208 1308
Fragments arranged by size:
1308 1208 833 46
BsiHKAI G_wGCw'C
Cuts at: 409 1187 1377 2169 409
Size: 778 190 792 1635
Fragments arranged by size:
1635 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 334 409 1020 1113 1187 1377 1439 2169 334
Size: 75 611 93 74 190 62 730 1560
Fragments arranged by size:
1560 730 611 190 93 75 74 62
BspEI T'CCGG_A
Cuts at: 2615 2615
Size: 3395
BspHI T'CATG_A
Cuts at: 2571 2571
Size: 3395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 445 659 961 1342 445
Size: 214 302 381 2498
Fragments arranged by size:
2498 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1687 1741 3225 3390 50
Size: 1637 54 1484 165 55
Fragments arranged by size:
1637 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1307 1307
Size: 3395
BsrFI r'CCGG_y
Cuts at: 1393 1574 1783 1393
Size: 181 209 3005
Fragments arranged by size:
3005 209 181
BssSI C'ACGA_G
Cuts at: 1666 2024 1666
Size: 358 3037
BstAPI GCAn_nnn'nTGC
Cuts at: 772 772
Size: 3395
BstBI TT'CG_AA
Cuts at: 1756 1756
Size: 3395
BstYI r'GATC_y
Cuts at: 1 420 887 910 1245 1491 2503 2589 2601
Size: 419 467 23 335 246 1012 86 12
Cuts at: 2601 2947 1
Size: 346 449
Fragments arranged by size:
1012 467 449 419 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1345 1526 1689 1345
Size: 181 163 3051
Fragments arranged by size:
3051 181 163
BtsI GCAGTG_nn'
Cuts at: 638 638
Size: 3395
DraI TTT'AAA
Cuts at: 125 194 394 125
Size: 69 200 3126
Fragments arranged by size:
3126 200 69
DrdI GACnn_nn'nnGTC
Cuts at: 1101 1959 1101
Size: 858 2537
EaeI y'GGCC_r
Cuts at: 980 1154 1545 1572 2917 980
Size: 174 391 27 1345 1458
Fragments arranged by size:
1458 1345 391 174 27
EagI C'GGCC_G
Cuts at: 980 980
Size: 3395
EarI CTCTTCn'nnn_
Cuts at: 1418 1628 1418
Size: 210 3185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1603 1913 2059 3205 1603
Size: 310 146 1146 1793
Fragments arranged by size:
1793 1146 310 146
EcoICRI GAG'CTC
Cuts at: 407 407
Size: 3395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 1652 2399 2667 1220
Size: 432 747 268 1948
Fragments arranged by size:
1948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 534 534
Size: 3395
EcoRI G'AATT_C
Cuts at: 399 399
Size: 3395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1414 1414
Size: 3395
FspI TGC'GCA
Cuts at: 1176 1176
Size: 3395
HaeII r_GCGC'y
Cuts at: 829 1077 2099 3078 829
Size: 248 1022 979 1146
Fragments arranged by size:
1146 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1237 1267 1269 1299 1237
Size: 30 2 30 3333
Fragments arranged by size:
3333 30 30 2
HincII GTy'rAC
Cuts at: 434 434
Size: 3395
HindIII A'AGCT_T
Cuts at: 298 298
Size: 3395
KasI G'GCGC_C
Cuts at: 825 1073 825
Size: 248 3147
KpnI G_GTAC'C
Cuts at: 258 415 258
Size: 157 3238
MluI A'CGCG_T
Cuts at: 2828 2828
Size: 3395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 223 2066 2250 223
Size: 1843 184 1368
Fragments arranged by size:
1843 1368 184
MscI TGG'CCA
Cuts at: 1156 1156
Size: 3395
MslI CAynn'nnrTG
Cuts at: 1384 1511 1384
Size: 127 3268
MspA1I CmG'CkG
Cuts at: 506 820 1180 2193 2438 506
Size: 314 360 1013 245 1463
Fragments arranged by size:
1463 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1576 1576
Size: 3395
NarI GG'CG_CC
Cuts at: 826 1074 826
Size: 248 3147
NcoI C'CATG_G
Cuts at: 107 107
Size: 3395
NgoMIV G'CCGG_C
Cuts at: 1574 1574
Size: 3395
NspI r_CATG'y
Cuts at: 448 948 1855 448
Size: 500 907 1988
Fragments arranged by size:
1988 907 500
PciI A'CATG_T
Cuts at: 1851 1851
Size: 3395
PmeI GTTT'AAAC
Cuts at: 394 394
Size: 3395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 3363
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 238 270 238
Size: 32 3363
PstI C_TGCA'G
Cuts at: 442 1127 442
Size: 685 2710
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3395
PvuII CAG'CTG
Cuts at: 820 1180 820
Size: 360 3035
RsrII CG'GwC_CG
Cuts at: 1590 1590
Size: 3395
SacI G_AGCT'C
Cuts at: 409 409
Size: 3395
SalI G'TCGA_C
Cuts at: 432 432
Size: 3395
SapI GCTCTTCn'nnn_
Cuts at: 1418 1628 1418
Size: 210 3185
SbfI CC_TGCA'GG
Cuts at: 442 442
Size: 3395
ScaI AGT'ACT
Cuts at: 2912 2912
Size: 3395
SfcI C'TryA_G
Cuts at: 33 386 438 1123 2116 2307 33
Size: 353 52 685 993 191 1121
Fragments arranged by size:
1121 993 685 353 191 52
SfoI GGC'GCC
Cuts at: 827 1075 827
Size: 248 3147
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3395
SmaI CCC'GGG
Cuts at: 417 417
Size: 3395
SmlI C'TyrA_G
Cuts at: 207 270 550 1465 1957 2219 207
Size: 63 280 915 492 262 1383
Fragments arranged by size:
1383 915 492 280 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3395
SphI G_CATG'C
Cuts at: 448 948 448
Size: 500 2895
StyI C'CwwG_G
Cuts at: 107 529 883 107
Size: 422 354 2619
Fragments arranged by size:
2619 422 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 978 1111 1570 1779 978
Size: 133 459 209 2594
Fragments arranged by size:
2594 459 209 133
TatI w'GTAC_w
Cuts at: 2910 2910
Size: 3395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3363
Tth111I GACn'n_nGTC
Cuts at: 1192 1192
Size: 3395
XbaI T'CTAG_A
Cuts at: 426 426
Size: 3395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 890 890
Size: 3395
XmaI C'CCGG_G
Cuts at: 415 415
Size: 3395
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI