Restriction map for
pF1KB0905
(Circular) (Six-base) MAPSORT of: pF1KB0905.seq Check: 7585 from: 1 to: 3392
pF1KB0905 3392 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 430 430
Size: 3392
Acc65I G'GTAC_C
Cuts at: 408 408
Size: 3392
AclI AA'CG_TT
Cuts at: 386 3232 386
Size: 2846 546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1217 1649 2396 2664 1217
Size: 432 747 268 1945
Fragments arranged by size:
1945 747 432 268
AflIII A'CryG_T
Cuts at: 744 1848 2825 744
Size: 1104 977 1311
Fragments arranged by size:
1311 1104 977
AgeI A'CCGG_T
Cuts at: 1780 1780
Size: 3392
AlwNI CAG_nnn'CTG
Cuts at: 697 2264 697
Size: 1567 1825
ApaLI G'TGCA_C
Cuts at: 2162 2162
Size: 3392
ApoI r'AATT_y
Cuts at: 396 2745 3001 396
Size: 2349 256 787
Fragments arranged by size:
2349 787 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3392
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3392
AvaI C'yCGr_G
Cuts at: 412 412
Size: 3392
AvrII C'CTAG_G
Cuts at: 159 159
Size: 3392
BamHI G'GATC_C
Cuts at: 417 417
Size: 3392
BanI G'GyrC_C
Cuts at: 408 822 1070 1105 408
Size: 414 248 35 2695
Fragments arranged by size:
2695 414 248 35
BanII G_rGCy'C
Cuts at: 406 1436 406
Size: 1030 2362
BbeI G_GCGC'C
Cuts at: 826 1074 826
Size: 248 3144
BbsI GAAGACnn'nnnn_
Cuts at: 294 294
Size: 3392
BciVI GTATCCnnnnn_n'
Cuts at: 1290 2051 3392 1290
Size: 761 1341 1290
Fragments arranged by size:
1341 1290 761
BclI T'GATC_A
Cuts at: 912 912
Size: 3392
BglII A'GATC_T
Cuts at: 113 907 2944 113
Size: 794 2037 561
Fragments arranged by size:
2037 794 561
BlpI GC'TnA_GC
Cuts at: 504 504
Size: 3392
Bme1580I G_kGCm'C
Cuts at: 1017 1110 2166 1017
Size: 93 1056 2243
Fragments arranged by size:
2243 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 335 604 685 1020 3164 335
Size: 269 81 335 2144 563
Fragments arranged by size:
2144 563 335 269 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1447 1939 2237 568
Size: 879 492 298 1723
Fragments arranged by size:
1723 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 262 3096 262
Size: 2834 558
BsaAI yAC'GTr
Cuts at: 745 745
Size: 3392
BsaBI GATnn'nnATC
Cuts at: 6 906 2943 6
Size: 900 2037 455
Fragments arranged by size:
2037 900 455
BsaHI Gr'CG_yC
Cuts at: 823 1071 1773 3269 823
Size: 248 702 1496 946
Fragments arranged by size:
1496 946 702 248
BsaWI w'CCGG_w
Cuts at: 581 1102 1780 2054 2201 2612 581
Size: 521 678 274 147 411 1361
Fragments arranged by size:
1361 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 171 133
Size: 38 3354
BseYI C'CCAG_C
Cuts at: 817 1575 2152 2648 817
Size: 758 577 496 1561
Fragments arranged by size:
1561 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 934 980 2188 104
Size: 830 46 1208 1308
Fragments arranged by size:
1308 1208 830 46
BsiHKAI G_wGCw'C
Cuts at: 286 406 1184 1374 2166 286
Size: 120 778 190 792 1512
Fragments arranged by size:
1512 792 778 190 120
Bsp1286I G_dGCh'C
Cuts at: 286 406 1017 1110 1184 1374 1436 2166 286
Size: 120 611 93 74 190 62 730 1512
Fragments arranged by size:
1512 730 611 190 120 93 74 62
BspEI T'CCGG_A
Cuts at: 2612 2612
Size: 3392
BspHI T'CATG_A
Cuts at: 2568 2568
Size: 3392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 656 958 1339 442
Size: 214 302 381 2495
Fragments arranged by size:
2495 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1684 1738 3222 3387 50
Size: 1634 54 1484 165 55
Fragments arranged by size:
1634 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1304 1304
Size: 3392
BsrFI r'CCGG_y
Cuts at: 1390 1571 1780 1390
Size: 181 209 3002
Fragments arranged by size:
3002 209 181
BssSI C'ACGA_G
Cuts at: 1663 2021 1663
Size: 358 3034
BstAPI GCAn_nnn'nTGC
Cuts at: 769 769
Size: 3392
BstBI TT'CG_AA
Cuts at: 1753 1753
Size: 3392
BstYI r'GATC_y
Cuts at: 1 113 236 417 884 907 1242 1488 2500
Size: 112 123 181 467 23 335 246 1012
Cuts at: 2500 2586 2598 2944 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 181 123
112 86 23 12
BtgI C'CryG_G
Cuts at: 119 119
Size: 3392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 138 1342 1523 1686 138
Size: 1204 181 163 1844
Fragments arranged by size:
1844 1204 181 163
BtsI GCAGTG_nn'
Cuts at: 635 635
Size: 3392
DraI TTT'AAA
Cuts at: 391 391
Size: 3392
DrdI GACnn_nn'nnGTC
Cuts at: 352 1098 1956 352
Size: 746 858 1788
Fragments arranged by size:
1788 858 746
EaeI y'GGCC_r
Cuts at: 977 1151 1542 1569 2914 977
Size: 174 391 27 1345 1455
Fragments arranged by size:
1455 1345 391 174 27
EagI C'GGCC_G
Cuts at: 977 977
Size: 3392
EarI CTCTTCn'nnn_
Cuts at: 1415 1625 1415
Size: 210 3182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1600 1910 2056 3202 1600
Size: 310 146 1146 1790
Fragments arranged by size:
1790 1146 310 146
EcoICRI GAG'CTC
Cuts at: 404 404
Size: 3392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1217 1649 2396 2664 1217
Size: 432 747 268 1945
Fragments arranged by size:
1945 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 190 531 190
Size: 341 3051
EcoRI G'AATT_C
Cuts at: 396 396
Size: 3392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1411 1411
Size: 3392
FspI TGC'GCA
Cuts at: 1173 1173
Size: 3392
HaeII r_GCGC'y
Cuts at: 826 1074 2096 3075 826
Size: 248 1022 979 1143
Fragments arranged by size:
1143 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 1234 1264 1266 1296 228
Size: 32 974 30 2 30 2324
Fragments arranged by size:
2324 974 32 30 30 2
HincII GTy'rAC
Cuts at: 431 431
Size: 3392
KasI G'GCGC_C
Cuts at: 822 1070 822
Size: 248 3144
KpnI G_GTAC'C
Cuts at: 412 412
Size: 3392
MluI A'CGCG_T
Cuts at: 2825 2825
Size: 3392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2247 2063
Size: 184 3208
MscI TGG'CCA
Cuts at: 1153 1153
Size: 3392
MslI CAynn'nnrTG
Cuts at: 1381 1508 1381
Size: 127 3265
MspA1I CmG'CkG
Cuts at: 249 503 817 1177 2190 2435 249
Size: 254 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 360 314 254 245
NaeI GCC'GGC
Cuts at: 1573 1573
Size: 3392
NarI GG'CG_CC
Cuts at: 823 1071 823
Size: 248 3144
NgoMIV G'CCGG_C
Cuts at: 1571 1571
Size: 3392
NspI r_CATG'y
Cuts at: 445 945 1852 445
Size: 500 907 1985
Fragments arranged by size:
1985 907 500
PciI A'CATG_T
Cuts at: 1848 1848
Size: 3392
PfoI T'CCnGG_A
Cuts at: 356 356
Size: 3392
PmeI GTTT'AAAC
Cuts at: 391 391
Size: 3392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 3360
PpuMI rG'GwC_Cy
Cuts at: 190 190
Size: 3392
PstI C_TGCA'G
Cuts at: 439 1124 439
Size: 685 2707
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3392
PvuII CAG'CTG
Cuts at: 817 1177 817
Size: 360 3032
RsrII CG'GwC_CG
Cuts at: 1587 1587
Size: 3392
SacI G_AGCT'C
Cuts at: 406 406
Size: 3392
SalI G'TCGA_C
Cuts at: 429 429
Size: 3392
SapI GCTCTTCn'nnn_
Cuts at: 1415 1625 1415
Size: 210 3182
SbfI CC_TGCA'GG
Cuts at: 439 439
Size: 3392
ScaI AGT'ACT
Cuts at: 2909 2909
Size: 3392
SfcI C'TryA_G
Cuts at: 33 435 1120 2113 2304 33
Size: 402 685 993 191 1121
Fragments arranged by size:
1121 993 685 402 191
SfoI GGC'GCC
Cuts at: 824 1072 824
Size: 248 3144
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3392
SmaI CCC'GGG
Cuts at: 414 414
Size: 3392
SmlI C'TyrA_G
Cuts at: 547 1462 1954 2216 547
Size: 915 492 262 1723
Fragments arranged by size:
1723 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3392
SphI G_CATG'C
Cuts at: 445 945 445
Size: 500 2892
StyI C'CwwG_G
Cuts at: 159 526 880 159
Size: 367 354 2671
Fragments arranged by size:
2671 367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 975 1108 1567 1776 975
Size: 133 459 209 2591
Fragments arranged by size:
2591 459 209 133
TatI w'GTAC_w
Cuts at: 2907 2907
Size: 3392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3360
Tth111I GACn'n_nGTC
Cuts at: 1189 1189
Size: 3392
XbaI T'CTAG_A
Cuts at: 423 423
Size: 3392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 887 887
Size: 3392
XmaI C'CCGG_G
Cuts at: 412 412
Size: 3392
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI