Restriction map for
pF1KB0487
(Circular) (Six-base) MAPSORT of: pF1KB0487.seq Check: 6211 from: 1 to: 4700
pF1KB0487 4700 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 581 1738 581
Size: 1157 3543
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 2525 2957 3704 3972 355
Size: 2170 432 747 268 1083
Fragments arranged by size:
2170 1083 747 432 268
AfeI AGC'GCT
Cuts at: 570 570
Size: 4700
AflIII A'CryG_T
Cuts at: 2052 3156 4133 2052
Size: 1104 977 2619
Fragments arranged by size:
2619 1104 977
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AleI CACnn'nnGTG
Cuts at: 886 1373 886
Size: 487 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 886 886
Size: 4700
AlwNI CAG_nnn'CTG
Cuts at: 376 2005 3572 376
Size: 1629 1567 1504
Fragments arranged by size:
1629 1567 1504
ApaLI G'TGCA_C
Cuts at: 3470 3470
Size: 4700
ApoI r'AATT_y
Cuts at: 199 253 461 1026 1704 4053 4309 199
Size: 54 208 565 678 2349 256 590
Fragments arranged by size:
2349 678 590 565 256 208 54
AseI AT'TA_AT
Cuts at: 20 484 20
Size: 464 4236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 1720 1720
Size: 4700
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4700
BanI G'GyrC_C
Cuts at: 1716 2130 2378 2413 1716
Size: 414 248 35 4003
Fragments arranged by size:
4003 414 248 35
BanII G_rGCy'C
Cuts at: 1092 1714 2744 1092
Size: 622 1030 3048
Fragments arranged by size:
3048 1030 622
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BbsI GAAGACnn'nnnn_
Cuts at: 1310 1390 1310
Size: 80 4620
BciVI GTATCCnnnnn_n'
Cuts at: 255 436 1101 2598 3359 4700 255
Size: 181 665 1497 761 1341 255
Fragments arranged by size:
1497 1341 761 665 255 181
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 2325 2418 3474 2325
Size: 93 1056 3551
Fragments arranged by size:
3551 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 194
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 2755 3247 3545 1876
Size: 879 492 298 3031
Fragments arranged by size:
3031 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1129 4404 1129
Size: 3275 1425
BsaAI yAC'GTr
Cuts at: 505 2053 505
Size: 1548 3152
BsaBI GATnn'nnATC
Cuts at: 6 895 2214 4251 6
Size: 889 1319 2037 455
Fragments arranged by size:
2037 1319 889 455
BsaHI Gr'CG_yC
Cuts at: 2131 2379 3081 4577 2131
Size: 248 702 1496 2254
Fragments arranged by size:
2254 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1889 2410 3088 3362 3509 3920 1889
Size: 521 678 274 147 411 2669
Fragments arranged by size:
2669 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 914 2125 2883 3460 3956 914
Size: 1211 758 577 496 1658
Fragments arranged by size:
1658 1211 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 996 1714 2492 2682 3474 996
Size: 718 778 190 792 2222
Fragments arranged by size:
2222 792 778 718 190
BsmI GAATG_Cn'
Cuts at: 283 1193 283
Size: 910 3790
Bsp1286I G_dGCh'C
Cuts at: 996 1092 1714 2325 2418 2492 2682 2744 3474
Size: 96 622 611 93 74 190 62 730
Cuts at: 3474 996
Size: 2222
Fragments arranged by size:
2222 730 622 611 190 96 93 74
62
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 3876 3876
Size: 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1750 1964 2266 2647 1003
Size: 747 214 302 381 3056
Fragments arranged by size:
3056 747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 950 1259 2612 950
Size: 309 1353 3038
Fragments arranged by size:
3038 1353 309
BsrFI r'CCGG_y
Cuts at: 2698 2879 3088 2698
Size: 181 209 4310
Fragments arranged by size:
4310 209 181
BssSI C'ACGA_G
Cuts at: 1144 2971 3329 1144
Size: 1827 358 2515
Fragments arranged by size:
2515 1827 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstYI r'GATC_y
Cuts at: 1 1725 2192 2215 2550 2796 3808 3894 3906
Size: 1724 467 23 335 246 1012 86 12
Cuts at: 3906 4252 1
Size: 346 449
Fragments arranged by size:
1724 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 666 2650 2831 2994 666
Size: 1984 181 163 2372
Fragments arranged by size:
2372 1984 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 1943
Size: 4700
DraI TTT'AAA
Cuts at: 1025 1699 1025
Size: 674 4026
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3264 2406
Size: 858 3842
EaeI y'GGCC_r
Cuts at: 947 1031 2285 2459 2850 2877 4222 947
Size: 84 1254 174 391 27 1345 1425
Fragments arranged by size:
1425 1345 1254 391 174 84 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 1511 2723 2933 1511
Size: 1212 210 3278
Fragments arranged by size:
3278 1212 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 355 2525 2957 3704 3972 194
Size: 161 2170 432 747 268 922
Fragments arranged by size:
2170 922 747 432 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 686 686
Size: 4700
EcoO109I rG'GnC_Cy
Cuts at: 1839 1839
Size: 4700
EcoRI G'AATT_C
Cuts at: 461 1704 461
Size: 1243 3457
EcoRV GAT'ATC
Cuts at: 827 827
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 2719 1624
Size: 1095 3605
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 572 2134 2382 3404 4383 572
Size: 1562 248 1022 979 889
Fragments arranged by size:
1562 1022 979 889 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1377 1409 2542 2572 2574 2604 1377
Size: 32 1133 30 2 30 3473
Fragments arranged by size:
3473 1133 32 30 30 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
HindIII A'AGCT_T
Cuts at: 769 769
Size: 4700
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MfeI C'AATT_G
Cuts at: 1640 1640
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 969 1441 3371 3555 969
Size: 472 1930 184 2114
Fragments arranged by size:
2114 1930 472 184
MscI TGG'CCA
Cuts at: 949 1033 2461 949
Size: 84 1428 3188
Fragments arranged by size:
3188 1428 84
MslI CAynn'nnrTG
Cuts at: 112 886 1373 2689 2816 112
Size: 774 487 1316 127 1996
Fragments arranged by size:
1996 1316 774 487 127
MspA1I CmG'CkG
Cuts at: 321 366 817 1811 2125 2485 3498 3743 321
Size: 45 451 994 314 360 1013 245 1278
Fragments arranged by size:
1278 1013 994 451 360 314 245 45
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NspI r_CATG'y
Cuts at: 1042 1753 2253 3160 1042
Size: 711 500 907 2582
Fragments arranged by size:
2582 907 711 500
PasI CC'CwG_GG
Cuts at: 490 490
Size: 4700
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PmlI CAC'GTG
Cuts at: 505 505
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 745 777 3839 3871 745
Size: 32 3062 32 1574
Fragments arranged by size:
3062 1574 32 32
PstI C_TGCA'G
Cuts at: 822 1747 2432 822
Size: 925 685 3090
Fragments arranged by size:
3090 925 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 321 817 2125 2485 321
Size: 496 1308 360 2536
Fragments arranged by size:
2536 1308 496 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 818 1743 2428 3421 3612 33
Size: 785 925 685 993 191 1121
Fragments arranged by size:
1121 993 925 785 685 191
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 1855 2770 3262 3524 1855
Size: 915 492 262 3031
Fragments arranged by size:
3031 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1042 1753 2253 1042
Size: 711 500 3489
Fragments arranged by size:
3489 711 500
SspI AAT'ATT
Cuts at: 867 867
Size: 4700
StyI C'CwwG_G
Cuts at: 1834 2188 1834
Size: 354 4346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 972 2283 2416 2875 3084 898
Size: 74 1311 133 459 209 2514
Fragments arranged by size:
2514 1311 459 209 133 74
TatI w'GTAC_w
Cuts at: 4215 4215
Size: 4700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1099 1731 1099
Size: 632 4068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1206 2195 1206
Size: 989 3711
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
XmnI GAAnn'nnTTC
Cuts at: 465 1179 465
Size: 714 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI