Restriction map for
pF1KB0476
(Circular) (Six-base) MAPSORT of: pF1KB0476.seq Check: 111 from: 1 to: 6170
pF1KB0476 6170 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 2784 3208 2784
Size: 424 5746
Acc65I G'GTAC_C
Cuts at: 113 3186 113
Size: 3073 3097
AclI AA'CG_TT
Cuts at: 6010 6010
Size: 6170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 735 956 1057 2201 2592 3095 3995 4427
Size: 433 221 101 1144 391 503 900 432
Cuts at: 4427 5174 5442 302
Size: 747 268 1030
Fragments arranged by size:
1144 1030 900 747 503 433 432 391
268 221 101
AflII C'TTAA_G
Cuts at: 423 1156 2242 423
Size: 733 1086 4351
Fragments arranged by size:
4351 1086 733
AflIII A'CryG_T
Cuts at: 1263 1286 3522 4626 5603 1263
Size: 23 2236 1104 977 1830
Fragments arranged by size:
2236 1830 1104 977 23
AgeI A'CCGG_T
Cuts at: 4558 4558
Size: 6170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 968 1000 968
Size: 32 6138
AlwNI CAG_nnn'CTG
Cuts at: 2265 3475 5042 2265
Size: 1210 1567 3393
Fragments arranged by size:
3393 1567 1210
ApaLI G'TGCA_C
Cuts at: 382 4940 382
Size: 4558 1612
ApoI r'AATT_y
Cuts at: 757 835 967 1480 1669 1693 1707 1837 2920
Size: 78 132 513 189 24 14 130 1083
Cuts at: 2920 3042 3097 3174 5523 5779 757
Size: 122 55 77 2349 256 1148
Fragments arranged by size:
2349 1148 1083 513 256 189 132 130
122 78 77 55 24 14
AseI AT'TA_AT
Cuts at: 20 1232 20
Size: 1212 4958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6170
AvaI C'yCGr_G
Cuts at: 1208 3190 1208
Size: 1982 4188
BamHI G'GATC_C
Cuts at: 402 3195 402
Size: 2793 3377
BanI G'GyrC_C
Cuts at: 113 172 586 3186 3600 3848 3883 113
Size: 59 414 2600 414 248 35 2400
Fragments arranged by size:
2600 2400 414 414 248 59 35
BanII G_rGCy'C
Cuts at: 1209 1904 2537 3184 4214 1209
Size: 695 633 647 1030 3165
Fragments arranged by size:
3165 1030 695 647 633
BbeI G_GCGC'C
Cuts at: 176 3604 3852 176
Size: 3428 248 2494
Fragments arranged by size:
3428 2494 248
BbsI GAAGACnn'nnnn_
Cuts at: 290 2351 2598 290
Size: 2061 247 3862
Fragments arranged by size:
3862 2061 247
BbvCI CC'TCA_GC
Cuts at: 2258 2258
Size: 6170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6136
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2979 4068 4829 6170 1758
Size: 1221 1089 761 1341 1758
Fragments arranged by size:
1758 1341 1221 1089 761
BclI T'GATC_A
Cuts at: 3690 3690
Size: 6170
BfrBI ATG'CAT
Cuts at: 1930 1930
Size: 6170
BglII A'GATC_T
Cuts at: 3685 5722 3685
Size: 2037 4133
BlpI GC'TnA_GC
Cuts at: 3282 3282
Size: 6170
Bme1580I G_kGCm'C
Cuts at: 386 591 622 3795 3888 4944 386
Size: 205 31 3173 93 1056 1612
Fragments arranged by size:
3173 1612 1056 205 93 31
BmgBI CAC'GTC
Cuts at: 2620 2620
Size: 6170
BmrI ACTGGGnnnn_n'
Cuts at: 1596 2838 3382 3463 3798 5942 1596
Size: 1242 544 81 335 2144 1824
Fragments arranged by size:
2144 1824 1242 544 335 81
BmtI G_CTAG'C
Cuts at: 124 1948 124
Size: 1824 4346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4537 4537
Size: 6170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 805 2551 723
Size: 82 1746 4342
Fragments arranged by size:
4342 1746 82
Bpu10I CC'TnA_GC
Cuts at: 2258 2258
Size: 6170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1918 1974 2664 3346 4225 4717 5015 1310
Size: 608 56 690 682 879 492 298 2465
Fragments arranged by size:
2465 879 690 682 608 492 298 56
BsaI GGTCTCn'nnnn_
Cuts at: 5874 5874
Size: 6170
BsaAI yAC'GTr
Cuts at: 1312 3523 1312
Size: 2211 3959
BsaBI GATnn'nnATC
Cuts at: 6 3684 5721 6
Size: 3678 2037 455
Fragments arranged by size:
3678 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 3601 3849 4551 6047 173
Size: 3428 248 702 1496 296
Fragments arranged by size:
3428 1496 702 296 248
BsaWI w'CCGG_w
Cuts at: 189 1623 3359 3880 4558 4832 4979 5390 189
Size: 1434 1736 521 678 274 147 411 969
Fragments arranged by size:
1736 1434 969 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 261 291 2246 2276 184
Size: 30 47 30 1955 30 4078
Fragments arranged by size:
4078 1955 47 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1894 1894
Size: 6170
BseYI C'CCAG_C
Cuts at: 148 1169 2846 3595 4353 4930 5426 148
Size: 1021 1677 749 758 577 496 892
Fragments arranged by size:
1677 1021 892 758 749 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 1334 2260 118
Size: 1216 926 4028
Fragments arranged by size:
4028 1216 926
BsiEI CG_ry'CG
Cuts at: 104 3712 3758 4966 104
Size: 3608 46 1208 1308
Fragments arranged by size:
3608 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 386 1457 1904 2537 3184 3962 4152 4944 386
Size: 1071 447 633 647 778 190 792 1612
Fragments arranged by size:
1612 1071 792 778 647 633 447 190
BsmI GAATG_Cn'
Cuts at: 663 1365 663
Size: 702 5468
Bsp1286I G_dGCh'C
Cuts at: 386 591 622 1209 1457 1904 2537 3184 3795
Size: 205 31 587 248 447 633 647 611
Cuts at: 3795 3888 3962 4152 4214 4944 386
Size: 93 74 190 62 730 1612
Fragments arranged by size:
1612 730 647 633 611 587 447 248
205 190 93 74 62 31
BspEI T'CCGG_A
Cuts at: 189 5390 189
Size: 5201 969
BspHI T'CATG_A
Cuts at: 570 5346 570
Size: 4776 1394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3220 3434 3736 4117 3220
Size: 214 302 381 5273
Fragments arranged by size:
5273 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 4462 4516 6000 6165 50
Size: 111 4301 54 1484 165 55
Fragments arranged by size:
4301 1484 165 111 55 54
BsrDI GCAATG_nn'
Cuts at: 958 4082 958
Size: 3124 3046
BsrFI r'CCGG_y
Cuts at: 116 4168 4349 4558 116
Size: 4052 181 209 1728
Fragments arranged by size:
4052 1728 209 181
BssSI C'ACGA_G
Cuts at: 4441 4799 4441
Size: 358 5812
BstAPI GCAn_nnn'nTGC
Cuts at: 2947 3547 2947
Size: 600 5570
BstBI TT'CG_AA
Cuts at: 4531 4531
Size: 6170
BstXI CCAn_nnnn'nTGG
Cuts at: 867 2994 867
Size: 2127 4043
BstYI r'GATC_y
Cuts at: 1 402 3195 3662 3685 4020 4266 5278 5364
Size: 401 2793 467 23 335 246 1012 86
Cuts at: 5364 5376 5722 1
Size: 12 346 449
Fragments arranged by size:
2793 1012 467 449 401 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 353 1396 2295 2714 353
Size: 1043 899 419 3809
Fragments arranged by size:
3809 1043 899 419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4120 4301 4464 4120
Size: 181 163 5826
Fragments arranged by size:
5826 181 163
BtsI GCAGTG_nn'
Cuts at: 142 3413 142
Size: 3271 2899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2454 2489 2454
Size: 35 6135
DraI TTT'AAA
Cuts at: 1380 1674 2891 3169 1380
Size: 294 1217 278 4381
Fragments arranged by size:
4381 1217 294 278
DrdI GACnn_nn'nnGTC
Cuts at: 3876 4734 3876
Size: 858 5312
EaeI y'GGCC_r
Cuts at: 314 3755 3929 4320 4347 5692 314
Size: 3441 174 391 27 1345 792
Fragments arranged by size:
3441 1345 792 391 174 27
EagI C'GGCC_G
Cuts at: 3755 3755
Size: 6170
EarI CTCTTCn'nnn_
Cuts at: 1442 1580 2542 2738 4193 4403 1442
Size: 138 962 196 1455 210 3209
Fragments arranged by size:
3209 1455 962 210 196 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4378 4688 4834 5980 4378
Size: 310 146 1146 4568
Fragments arranged by size:
4568 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1902 2535 3182 1902
Size: 633 647 4890
Fragments arranged by size:
4890 647 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 723 735 805 956 1057 2201 2551 2592
Size: 421 12 70 151 101 1144 350 41
Cuts at: 2592 3095 3995 4427 5174 5442 302
Size: 503 900 432 747 268 1030
Fragments arranged by size:
1144 1030 900 747 503 432 421 350
268 151 101 70 41 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1447 2426 1447
Size: 979 5191
EcoO109I rG'GnC_Cy
Cuts at: 1132 1321 3309 1132
Size: 189 1988 3993
Fragments arranged by size:
3993 1988 189
EcoRI G'AATT_C
Cuts at: 3097 3174 3097
Size: 77 6093
EcoRV GAT'ATC
Cuts at: 2913 2913
Size: 6170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 352 1015 4189 352
Size: 663 3174 2333
Fragments arranged by size:
3174 2333 663
FspI TGC'GCA
Cuts at: 2578 3951 2578
Size: 1373 4797
HaeII r_GCGC'y
Cuts at: 176 198 3604 3852 4874 5853 176
Size: 22 3406 248 1022 979 493
Fragments arranged by size:
3406 1022 979 493 248 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1947 1979 2639 2671 2825 2857 4012
Size: 32 1566 32 660 32 154 32 1155
Cuts at: 4012 4042 4044 4074 349
Size: 30 2 30 2445
Fragments arranged by size:
2445 1566 1155 660 154 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2785 3209 2785
Size: 424 5746
HindIII A'AGCT_T
Cuts at: 2699 2699
Size: 6170
KasI G'GCGC_C
Cuts at: 172 3600 3848 172
Size: 3428 248 2494
Fragments arranged by size:
3428 2494 248
KpnI G_GTAC'C
Cuts at: 117 3190 117
Size: 3073 3097
MfeI C'AATT_G
Cuts at: 715 715
Size: 6170
MluI A'CGCG_T
Cuts at: 5603 5603
Size: 6170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 884 2994 4841 5025 380
Size: 504 2110 1847 184 1525
Fragments arranged by size:
2110 1847 1525 504 184
MscI TGG'CCA
Cuts at: 316 3931 316
Size: 3615 2555
MslI CAynn'nnrTG
Cuts at: 434 1234 2147 4159 4286 434
Size: 800 913 2012 127 2318
Fragments arranged by size:
2318 2012 913 800 127
MspA1I CmG'CkG
Cuts at: 1462 2394 2612 3281 3595 3955 4968 5213 1462
Size: 932 218 669 314 360 1013 245 2419
Fragments arranged by size:
2419 1013 932 669 360 314 245 218
NaeI GCC'GGC
Cuts at: 4351 4351
Size: 6170
NarI GG'CG_CC
Cuts at: 173 3601 3849 173
Size: 3428 248 2494
Fragments arranged by size:
3428 2494 248
NcoI C'CATG_G
Cuts at: 2295 2714 2295
Size: 419 5751
NdeI CA'TA_TG
Cuts at: 443 1932 443
Size: 1489 4681
NgoMIV G'CCGG_C
Cuts at: 4349 4349
Size: 6170
NheI G'CTAG_C
Cuts at: 120 1944 120
Size: 1824 4346
NsiI A_TGCA'T
Cuts at: 1932 1932
Size: 6170
NspI r_CATG'y
Cuts at: 511 1267 1290 2152 2365 2505 3223 3723 4630
Size: 756 23 862 213 140 718 500 907
Cuts at: 4630 511
Size: 2051
Fragments arranged by size:
2051 907 862 756 718 500 213 140
23
PciI A'CATG_T
Cuts at: 1263 1286 4626 1263
Size: 23 3340 2807
Fragments arranged by size:
3340 2807 23
PfoI T'CCnGG_A
Cuts at: 1134 1134
Size: 6170
PmeI GTTT'AAAC
Cuts at: 3169 3169
Size: 6170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5309 5341 5309
Size: 32 6138
PpuMI rG'GwC_Cy
Cuts at: 1132 1321 1132
Size: 189 5981
PstI C_TGCA'G
Cuts at: 3217 3902 3217
Size: 685 5485
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6170
PvuII CAG'CTG
Cuts at: 2394 3595 3955 2394
Size: 1201 360 4609
Fragments arranged by size:
4609 1201 360
RsrII CG'GwC_CG
Cuts at: 4365 4365
Size: 6170
SacI G_AGCT'C
Cuts at: 1904 2537 3184 1904
Size: 633 647 4890
Fragments arranged by size:
4890 647 633
SalI G'TCGA_C
Cuts at: 2783 3207 2783
Size: 424 5746
SapI GCTCTTCn'nnn_
Cuts at: 2542 4193 4403 2542
Size: 1651 210 4309
Fragments arranged by size:
4309 1651 210
SbfI CC_TGCA'GG
Cuts at: 3217 3217
Size: 6170
ScaI AGT'ACT
Cuts at: 332 5687 332
Size: 5355 815
SfcI C'TryA_G
Cuts at: 33 728 3213 3898 4891 5082 33
Size: 695 2485 685 993 191 1121
Fragments arranged by size:
2485 1121 993 695 685 191
SfoI GGC'GCC
Cuts at: 174 3602 3850 174
Size: 3428 248 2494
Fragments arranged by size:
3428 2494 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6170
SmaI CCC'GGG
Cuts at: 3192 3192
Size: 6170
SmlI C'TyrA_G
Cuts at: 423 1156 1325 1897 1953 2242 2643 3325 4240
Size: 733 169 572 56 289 401 682 915
Cuts at: 4240 4732 4994 423
Size: 492 262 1599
Fragments arranged by size:
1599 915 733 682 572 492 401 289
262 169 56
SpeI A'CTAG_T
Cuts at: 67 532 67
Size: 465 5705
SphI G_CATG'C
Cuts at: 511 2152 3223 3723 511
Size: 1641 1071 500 2958
Fragments arranged by size:
2958 1641 1071 500
SspI AAT'ATT
Cuts at: 414 1427 1468 414
Size: 1013 41 5116
Fragments arranged by size:
5116 1013 41
StuI AGG'CCT
Cuts at: 2248 2749 2248
Size: 501 5669
StyI C'CwwG_G
Cuts at: 2295 2582 2714 2903 2953 3304 3658 2295
Size: 287 132 189 50 351 354 4807
Fragments arranged by size:
4807 354 351 287 189 132 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1261 3109 3753 3886 4345 4554 1261
Size: 1848 644 133 459 209 2877
Fragments arranged by size:
2877 1848 644 459 209 133
TatI w'GTAC_w
Cuts at: 330 1604 2156 5685 330
Size: 1274 552 3529 815
Fragments arranged by size:
3529 1274 815 552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 184 216 5687 5719 184
Size: 32 5471 32 635
Fragments arranged by size:
5471 635 32 32
Tth111I GACn'n_nGTC
Cuts at: 3967 3967
Size: 6170
XbaI T'CTAG_A
Cuts at: 3201 3201
Size: 6170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2589 3665 2589
Size: 1076 5094
XmaI C'CCGG_G
Cuts at: 3190 3190
Size: 6170
XmnI GAAnn'nnTTC
Cuts at: 1697 2288 3080 1697
Size: 591 792 4787
Fragments arranged by size:
4787 792 591
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BglI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII FseI FspAI HpaI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI