Restriction map for
pF1KB0468
(Circular) (Six-base) MAPSORT of: pF1KB0468.seq Check: 9648 from: 1 to: 6065
pF1KB0468 6065 bp
With 182 enzymes: SgfI *
June 14, 2010 14:44 ..
AccI GT'mk_AC
Cuts at: 3103 3103
Size: 6065
Acc65I G'GTAC_C
Cuts at: 3081 3081
Size: 6065
AclI AA'CG_TT
Cuts at: 5905 5905
Size: 6065
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 313 1113 1922 2071 2662 2983 3890 4322
Size: 155 800 809 149 591 321 907 432
Cuts at: 4322 5069 5337 158
Size: 747 268 886
Fragments arranged by size:
907 886 809 800 747 591 432 321
268 155 149
AfeI AGC'GCT
Cuts at: 2306 2306
Size: 6065
AflIII A'CryG_T
Cuts at: 3417 4521 5498 3417
Size: 1104 977 3984
Fragments arranged by size:
3984 1104 977
AgeI A'CCGG_T
Cuts at: 4453 4453
Size: 6065
AhdI GACnn_n'nnGTC
Cuts at: 1806 1806
Size: 6065
AleI CACnn'nnGTG
Cuts at: 944 944
Size: 6065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 875 2651 875
Size: 1776 4289
AlwNI CAG_nnn'CTG
Cuts at: 406 3370 4937 406
Size: 2964 1567 1534
Fragments arranged by size:
2964 1567 1534
ApaLI G'TGCA_C
Cuts at: 781 4835 781
Size: 4054 2011
ApoI r'AATT_y
Cuts at: 532 1359 1520 1656 2112 2926 3069 5418 5674
Size: 827 161 136 456 814 143 2349 256
Cuts at: 5674 532
Size: 923
Fragments arranged by size:
2349 923 827 814 456 256 161 143
136
AseI AT'TA_AT
Cuts at: 20 20
Size: 6065
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6065
AvaI C'yCGr_G
Cuts at: 3085 3085
Size: 6065
BamHI G'GATC_C
Cuts at: 1159 3090 1159
Size: 1931 4134
BanI G'GyrC_C
Cuts at: 1147 1420 3081 3495 3743 3778 1147
Size: 273 1661 414 248 35 3434
Fragments arranged by size:
3434 1661 414 273 248 35
BanII G_rGCy'C
Cuts at: 619 883 3079 4109 619
Size: 264 2196 1030 2575
Fragments arranged by size:
2575 2196 1030 264
BbeI G_GCGC'C
Cuts at: 3499 3747 3499
Size: 248 5817
BbsI GAAGACnn'nnnn_
Cuts at: 2089 2089
Size: 6065
BbvCI CC'TCA_GC
Cuts at: 2282 2282
Size: 6065
BciVI GTATCCnnnnn_n'
Cuts at: 1838 3963 4724 6065 1838
Size: 2125 761 1341 1838
Fragments arranged by size:
2125 1838 1341 761
BclI T'GATC_A
Cuts at: 3585 3585
Size: 6065
BfrBI ATG'CAT
Cuts at: 2628 2901 2628
Size: 273 5792
BglII A'GATC_T
Cuts at: 3580 5617 3580
Size: 2037 4028
BlpI GC'TnA_GC
Cuts at: 2421 3177 2421
Size: 756 5309
Bme1580I G_kGCm'C
Cuts at: 785 1541 3690 3783 4839 785
Size: 756 2149 93 1056 2011
Fragments arranged by size:
2149 2011 1056 756 93
BmrI ACTGGGnnnn_n'
Cuts at: 1181 1570 3277 3358 3693 5837 1181
Size: 389 1707 81 335 2144 1409
Fragments arranged by size:
2144 1707 1409 389 335 81
BmtI G_CTAG'C
Cuts at: 872 872
Size: 6065
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4432 4432
Size: 6065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 941 948 2574 828
Size: 113 7 1626 4319
Fragments arranged by size:
4319 1626 113 7
Bpu10I CC'TnA_GC
Cuts at: 2282 2282
Size: 6065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 609 876 1008 1199 1679 2512 2568 3241
Size: 337 267 132 191 480 833 56 673
Cuts at: 3241 4120 4612 4910 272
Size: 879 492 298 1427
Fragments arranged by size:
1427 879 833 673 492 480 337 298
267 191 132 56
BsaI GGTCTCn'nnnn_
Cuts at: 106 1721 5769 106
Size: 1615 4048 402
Fragments arranged by size:
4048 1615 402
BsaAI yAC'GTr
Cuts at: 3418 3418
Size: 6065
BsaBI GATnn'nnATC
Cuts at: 6 1826 3579 5616 6
Size: 1820 1753 2037 455
Fragments arranged by size:
2037 1820 1753 455
BsaHI Gr'CG_yC
Cuts at: 632 3496 3744 4446 5942 632
Size: 2864 248 702 1496 755
Fragments arranged by size:
2864 1496 755 702 248
BsaWI w'CCGG_w
Cuts at: 262 1830 3053 3254 3775 4453 4727 4874 5285
Size: 1568 1223 201 521 678 274 147 411
Cuts at: 5285 262
Size: 1042
Fragments arranged by size:
1568 1223 1042 678 521 411 274 201
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 797 875 2179 797
Size: 78 1304 4683
Fragments arranged by size:
4683 1304 78
BseYI C'CCAG_C
Cuts at: 3490 4248 4825 5321 3490
Size: 758 577 496 4234
Fragments arranged by size:
4234 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 6065
BsiEI CG_ry'CG
Cuts at: 104 1720 3607 3653 4861 104
Size: 1616 1887 46 1208 1308
Fragments arranged by size:
1887 1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 619 785 1033 1215 1695 2124 3079 3857 4047
Size: 166 248 182 480 429 955 778 190
Cuts at: 4047 4839 619
Size: 792 1845
Fragments arranged by size:
1845 955 792 778 480 429 248 190
182 166
BsmI GAATG_Cn'
Cuts at: 1407 1407
Size: 6065
Bsp1286I G_dGCh'C
Cuts at: 619 785 883 1033 1215 1541 1695 2124 3079
Size: 166 98 150 182 326 154 429 955
Cuts at: 3079 3690 3783 3857 4047 4109 4839 619
Size: 611 93 74 190 62 730 1845
Fragments arranged by size:
1845 955 730 611 429 326 190 182
166 154 150 98 93 74 62
BspEI T'CCGG_A
Cuts at: 5285 5285
Size: 6065
BspHI T'CATG_A
Cuts at: 669 2720 5241 669
Size: 2051 2521 1493
Fragments arranged by size:
2521 2051 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2927 3115 3329 3631 4012 2927
Size: 188 214 302 381 4980
Fragments arranged by size:
4980 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 3042 4357 4411 5895 6060 50
Size: 2992 1315 54 1484 165 55
Fragments arranged by size:
2992 1484 1315 165 55 54
BsrDI GCAATG_nn'
Cuts at: 879 1168 2987 3977 879
Size: 289 1819 990 2967
Fragments arranged by size:
2967 1819 990 289
BsrFI r'CCGG_y
Cuts at: 4063 4244 4453 4063
Size: 181 209 5675
Fragments arranged by size:
5675 209 181
BssHII G'CGCG_C
Cuts at: 639 639
Size: 6065
BssSI C'ACGA_G
Cuts at: 2161 2300 4336 4694 2161
Size: 139 2036 358 3532
Fragments arranged by size:
3532 2036 358 139
BstAPI GCAn_nnn'nTGC
Cuts at: 3442 3442
Size: 6065
BstBI TT'CG_AA
Cuts at: 1048 4426 1048
Size: 3378 2687
BstXI CCAn_nnnn'nTGG
Cuts at: 1095 2889 1095
Size: 1794 4271
BstYI r'GATC_y
Cuts at: 1 353 988 1063 1099 1159 1891 3090 3557
Size: 352 635 75 36 60 732 1199 467
Cuts at: 3557 3580 3915 4161 5173 5259 5271 5617 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1199 1012 732 635 467 449 352 346
335 246 86 75 60 36 23 12
BtgI C'CryG_G
Cuts at: 107 419 107
Size: 312 5753
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2223 4015 4196 4359 2223
Size: 1792 181 163 3929
Fragments arranged by size:
3929 1792 181 163
BtsI GCAGTG_nn'
Cuts at: 939 951 1136 2003 3308 939
Size: 12 185 867 1305 3696
Fragments arranged by size:
3696 1305 867 185 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2969 3004 2969
Size: 35 6030
DraI TTT'AAA
Cuts at: 1431 2478 3064 1431
Size: 1047 586 4432
Fragments arranged by size:
4432 1047 586
DrdI GACnn_nn'nnGTC
Cuts at: 164 3771 4629 164
Size: 3607 858 1600
Fragments arranged by size:
3607 1600 858
EaeI y'GGCC_r
Cuts at: 549 1275 3650 3824 4215 4242 5587 549
Size: 726 2375 174 391 27 1345 1027
Fragments arranged by size:
2375 1345 1027 726 391 174 27
EagI C'GGCC_G
Cuts at: 3650 3650
Size: 6065
EarI CTCTTCn'nnn_
Cuts at: 2612 4088 4298 2612
Size: 1476 210 4379
Fragments arranged by size:
4379 1476 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4273 4583 4729 5875 4273
Size: 310 146 1146 4463
Fragments arranged by size:
4463 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 3077 617
Size: 2460 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 313 828 941 948 1113 1922 2071 2574
Size: 155 515 113 7 165 809 149 503
Cuts at: 2574 2662 2983 3890 4322 5069 5337 158
Size: 88 321 907 432 747 268 886
Fragments arranged by size:
907 886 809 747 515 503 432 321
268 165 155 149 113 88 7
EcoO109I rG'GnC_Cy
Cuts at: 202 1221 3204 202
Size: 1019 1983 3063
Fragments arranged by size:
3063 1983 1019
EcoRI G'AATT_C
Cuts at: 1656 2926 3069 1656
Size: 1270 143 4652
Fragments arranged by size:
4652 1270 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1886 4084 1886
Size: 2198 3867
FspI TGC'GCA
Cuts at: 3846 3846
Size: 6065
HaeII r_GCGC'y
Cuts at: 2308 3499 3747 4769 5748 2308
Size: 1191 248 1022 979 2625
Fragments arranged by size:
2625 1191 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 1677 1709 1792 1824 2681 2713 3907
Size: 32 1275 32 83 32 857 32 1194
Cuts at: 3907 3937 3939 3969 370
Size: 30 2 30 2466
Fragments arranged by size:
2466 1275 1194 857 83 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 246 2265 3104 246
Size: 2019 839 3207
Fragments arranged by size:
3207 2019 839
KasI G'GCGC_C
Cuts at: 3495 3743 3495
Size: 248 5817
KpnI G_GTAC'C
Cuts at: 3085 3085
Size: 6065
MluI A'CGCG_T
Cuts at: 5498 5498
Size: 6065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1465 1764 4736 4920 1465
Size: 299 2972 184 2610
Fragments arranged by size:
2972 2610 299 184
MscI TGG'CCA
Cuts at: 551 1277 3826 551
Size: 726 2549 2790
Fragments arranged by size:
2790 2549 726
MslI CAynn'nnrTG
Cuts at: 944 2719 4054 4181 944
Size: 1775 1335 127 2828
Fragments arranged by size:
2828 1775 1335 127
MspA1I CmG'CkG
Cuts at: 525 1568 1973 3009 3176 3490 3850 4863 5108
Size: 1043 405 1036 167 314 360 1013 245
Cuts at: 5108 525
Size: 1482
Fragments arranged by size:
1482 1043 1036 1013 405 360 314 245
167
NaeI GCC'GGC
Cuts at: 4246 4246
Size: 6065
NarI GG'CG_CC
Cuts at: 3496 3744 3496
Size: 248 5817
NcoI C'CATG_G
Cuts at: 107 419 107
Size: 312 5753
NgoMIV G'CCGG_C
Cuts at: 4244 4244
Size: 6065
NheI G'CTAG_C
Cuts at: 868 868
Size: 6065
NsiI A_TGCA'T
Cuts at: 2630 2903 2630
Size: 273 5792
NspI r_CATG'y
Cuts at: 818 868 3118 3618 4525 818
Size: 50 2250 500 907 2358
Fragments arranged by size:
2358 2250 907 500 50
PasI CC'CwG_GG
Cuts at: 198 798 198
Size: 600 5465
PciI A'CATG_T
Cuts at: 4521 4521
Size: 6065
PflMI CCAn_nnn'nTGG
Cuts at: 2758 2758
Size: 6065
PfoI T'CCnGG_A
Cuts at: 2469 2469
Size: 6065
PmeI GTTT'AAAC
Cuts at: 3064 3064
Size: 6065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 5204
Size: 32 6033
PpuMI rG'GwC_Cy
Cuts at: 202 1221 202
Size: 1019 5046
PstI C_TGCA'G
Cuts at: 946 2696 3112 3797 946
Size: 1750 416 685 3214
Fragments arranged by size:
3214 1750 685 416
PvuI CG_AT'CG
Cuts at: 104 1720 104
Size: 1616 4449
PvuII CAG'CTG
Cuts at: 525 1973 3009 3490 3850 525
Size: 1448 1036 481 360 2740
Fragments arranged by size:
2740 1448 1036 481 360
RsrII CG'GwC_CG
Cuts at: 4260 4260
Size: 6065
SacI G_AGCT'C
Cuts at: 619 3079 619
Size: 2460 3605
SalI G'TCGA_C
Cuts at: 3102 3102
Size: 6065
SapI GCTCTTCn'nnn_
Cuts at: 4088 4298 4088
Size: 210 5855
SbfI CC_TGCA'GG
Cuts at: 3112 3112
Size: 6065
ScaI AGT'ACT
Cuts at: 285 1092 5582 285
Size: 807 4490 768
Fragments arranged by size:
4490 807 768
SexAI A'CCwGG_T
Cuts at: 2906 2906
Size: 6065
SfcI C'TryA_G
Cuts at: 33 206 407 942 1107 1680 2692 3108 3793
Size: 173 201 535 165 573 1012 416 685
Cuts at: 3793 4786 4977 33
Size: 993 191 1121
Fragments arranged by size:
1121 1012 993 685 573 535 416 201
191 173 165
SfoI GGC'GCC
Cuts at: 3497 3745 3497
Size: 248 5817
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6065
SmaI CCC'GGG
Cuts at: 3087 3087
Size: 6065
SmlI C'TyrA_G
Cuts at: 287 588 891 1023 1214 1694 2491 2547 3220
Size: 301 303 132 191 480 797 56 673
Cuts at: 3220 4135 4627 4889 287
Size: 915 492 262 1463
Fragments arranged by size:
1463 915 797 673 492 480 303 301
262 191 132 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6065
SphI G_CATG'C
Cuts at: 3118 3618 3118
Size: 500 5565
SspI AAT'ATT
Cuts at: 1014 1287 1014
Size: 273 5792
StyI C'CwwG_G
Cuts at: 107 419 3014 3199 3553 107
Size: 312 2595 185 354 2619
Fragments arranged by size:
2619 2595 354 312 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 232 811 2250 3648 3781 4240 4449 232
Size: 579 1439 1398 133 459 209 1848
Fragments arranged by size:
1848 1439 1398 579 459 209 133
TatI w'GTAC_w
Cuts at: 283 1090 1165 1295 5580 283
Size: 807 75 130 4285 768
Fragments arranged by size:
4285 807 768 130 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 1520 1552 1562 1594 1735 1767 5582
Size: 32 1343 32 10 32 141 32 3815
Cuts at: 5582 5614 145
Size: 32 596
Fragments arranged by size:
3815 1343 596 141 32 32 32 32
32 10
Tth111I GACn'n_nGTC
Cuts at: 2605 3862 2605
Size: 1257 4808
XbaI T'CTAG_A
Cuts at: 144 1810 3096 144
Size: 1666 1286 3113
Fragments arranged by size:
3113 1666 1286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1684 2904 3560 1684
Size: 1220 656 4189
Fragments arranged by size:
4189 1220 656
XmaI C'CCGG_G
Cuts at: 3085 3085
Size: 6065
XmnI GAAnn'nnTTC
Cuts at: 1844 1881 1844
Size: 37 6028
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BsaXI BsiWI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI