Restriction map for
pF1KB0465
(Circular) (Six-base) MAPSORT of: pF1KB0465.seq Check: 2581 from: 1 to: 4547
pF1KB0465 4547 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 302 302
Size: 4547
AccI GT'mk_AC
Cuts at: 1028 1585 1028
Size: 557 3990
Acc65I G'GTAC_C
Cuts at: 362 1563 362
Size: 1201 3346
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 2372 2804 3551 3819 544
Size: 1828 432 747 268 1272
Fragments arranged by size:
1828 1272 747 432 268
AflIII A'CryG_T
Cuts at: 515 1899 3003 3980 515
Size: 1384 1104 977 1082
Fragments arranged by size:
1384 1104 1082 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1066 1066
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 132 345 452 1371 1852 3419 132
Size: 213 107 919 481 1567 1260
Fragments arranged by size:
1567 1260 919 481 213 107
ApaLI G'TGCA_C
Cuts at: 1096 3317 1096
Size: 2221 2326
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 1299 1331 1424 1567 1299
Size: 32 93 143 4279
Fragments arranged by size:
4279 143 93 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 272 305 272
Size: 33 4514
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 362 1563 1977 2225 2260 362
Size: 1201 414 248 35 2649
Fragments arranged by size:
2649 1201 414 248 35
BanII G_rGCy'C
Cuts at: 1300 1447 1561 2591 1300
Size: 147 114 1030 3256
Fragments arranged by size:
3256 1030 147 114
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbsI GAAGACnn'nnnn_
Cuts at: 705 957 705
Size: 252 4295
BbvCI CC'TCA_GC
Cuts at: 211 211
Size: 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4513
BciVI GTATCCnnnnn_n'
Cuts at: 300 2445 3206 4547 300
Size: 2145 761 1341 300
Fragments arranged by size:
2145 1341 761 300
BclI T'GATC_A
Cuts at: 971 2067 971
Size: 1096 3451
BglI GCCn_nnn'nGGC
Cuts at: 1131 1345 1131
Size: 214 4333
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1242 1659 1242
Size: 417 4130
Bme1580I G_kGCm'C
Cuts at: 1100 2172 2265 3321 1100
Size: 1072 93 1056 2326
Fragments arranged by size:
2326 1072 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1759 1840 2175 4319 1759
Size: 81 335 2144 1987
Fragments arranged by size:
2144 1987 335 81
BmtI G_CTAG'C
Cuts at: 1486 1486
Size: 4547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 945 1392 2914 945
Size: 447 1522 2578
Fragments arranged by size:
2578 1522 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 376 1171 291
Size: 85 795 3667
Fragments arranged by size:
3667 795 85
Bpu10I CC'TnA_GC
Cuts at: 211 211
Size: 4547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3094 3392 1723
Size: 879 492 298 2878
Fragments arranged by size:
2878 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 933 1066 4251 933
Size: 133 3185 1229
Fragments arranged by size:
3185 1229 133
BsaAI yAC'GTr
Cuts at: 516 1900 516
Size: 1384 3163
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 299 1978 2226 2928 4424 299
Size: 1679 248 702 1496 422
Fragments arranged by size:
1679 1496 702 422 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 195 937 1004 136
Size: 59 742 67 3679
Fragments arranged by size:
3679 742 67 59
BseYI C'CCAG_C
Cuts at: 741 862 1206 1972 2730 3307 3803 741
Size: 121 344 766 758 577 496 1485
Fragments arranged by size:
1485 766 758 577 496 344 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 4547
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1100 1561 2339 2529 3321 1100
Size: 461 778 190 792 2326
Fragments arranged by size:
2326 792 778 461 190
BsiWI C'GTAC_G
Cuts at: 512 512
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 1100 1300 1447 1561 2172 2265 2339 2529 2591
Size: 200 147 114 611 93 74 190 62
Cuts at: 2591 3321 1100
Size: 730 2326
Fragments arranged by size:
2326 730 611 200 190 147 114 93
74 62
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 716 3723 716
Size: 3007 1540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 911 1118 1487 1597 1811 2113 2494 911
Size: 207 369 110 214 302 381 2964
Fragments arranged by size:
2964 381 369 302 214 207 110
BsrBI CCG'CTC
Cuts at: 50 205 2839 2893 4377 4542 50
Size: 155 2634 54 1484 165 55
Fragments arranged by size:
2634 1484 165 155 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 365 824 993 2545 2726 2935 365
Size: 459 169 1552 181 209 1977
Fragments arranged by size:
1977 1552 459 209 181 169
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 132 1110 1122 1924 132
Size: 978 12 802 2755
Fragments arranged by size:
2755 978 802 12
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 4547
BstYI r'GATC_y
Cuts at: 1 1409 1572 2039 2062 2397 2643 3655 3741
Size: 1408 163 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1408 1012 467 449 346 335 246 163
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 404 1177 404
Size: 773 3774
BtgI C'CryG_G
Cuts at: 440 1247 440
Size: 807 3740
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2497 2678 2841 2497
Size: 181 163 4203
Fragments arranged by size:
4203 181 163
BtsI GCAGTG_nn'
Cuts at: 998 1257 1790 998
Size: 259 533 3755
Fragments arranged by size:
3755 533 259
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 304 709 2253 3111 304
Size: 405 1544 858 1740
Fragments arranged by size:
1740 1544 858 405
EaeI y'GGCC_r
Cuts at: 995 1067 2132 2306 2697 2724 4069 995
Size: 72 1065 174 391 27 1345 1473
Fragments arranged by size:
1473 1345 1065 391 174 72 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 841 1288 2570 2780 841
Size: 447 1282 210 2608
Fragments arranged by size:
2608 1282 447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 811 2755 3065 3211 4357 811
Size: 1944 310 146 1146 1001
Fragments arranged by size:
1944 1146 1001 310 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 376 544 1171 2372 2804 3551 3819 291
Size: 85 168 627 1201 432 747 268 1019
Fragments arranged by size:
1201 1019 747 627 432 268 168 85
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 4547
EcoO109I rG'GnC_Cy
Cuts at: 456 1202 1276 1686 456
Size: 746 74 410 3317
Fragments arranged by size:
3317 746 410 74
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 133 681 1981 2229 3251 4230 133
Size: 548 1300 248 1022 979 450
Fragments arranged by size:
1300 1022 979 548 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 2389 2419 2421 2451 1225
Size: 32 1132 30 2 30 3321
Fragments arranged by size:
3321 1132 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1586 1190
Size: 396 4151
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 366 1567 366
Size: 1201 3346
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 725 3218 3402 200
Size: 525 2493 184 1345
Fragments arranged by size:
2493 1345 525 184
MscI TGG'CCA
Cuts at: 2308 2308
Size: 4547
MslI CAynn'nnrTG
Cuts at: 880 2536 2663 880
Size: 1656 127 2764
Fragments arranged by size:
2764 1656 127
MspA1I CmG'CkG
Cuts at: 1658 1972 2332 3345 3590 1658
Size: 314 360 1013 245 2615
Fragments arranged by size:
2615 1013 360 314 245
NaeI GCC'GGC
Cuts at: 826 995 2728 826
Size: 169 1733 2645
Fragments arranged by size:
2645 1733 169
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 1247 1247
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 824 993 2726 824
Size: 169 1733 2645
Fragments arranged by size:
2645 1733 169
NheI G'CTAG_C
Cuts at: 1482 1482
Size: 4547
NspI r_CATG'y
Cuts at: 1600 2100 3007 1600
Size: 500 907 3140
Fragments arranged by size:
3140 907 500
PciI A'CATG_T
Cuts at: 3003 3003
Size: 4547
PfoI T'CCnGG_A
Cuts at: 458 653 1391 458
Size: 195 738 3614
Fragments arranged by size:
3614 738 195
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 456 1276 456
Size: 820 3727
PstI C_TGCA'G
Cuts at: 1115 1264 1367 1594 2279 1115
Size: 149 103 227 685 3383
Fragments arranged by size:
3383 685 227 149 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1972 2332 1972
Size: 360 4187
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SanDI GG'GwC_CC
Cuts at: 456 456
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 1288 2570 2780 1288
Size: 1282 210 3055
Fragments arranged by size:
3055 1282 210
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 373 4064 373
Size: 3691 856
SexAI A'CCwGG_T
Cuts at: 1142 1142
Size: 4547
SfcI C'TryA_G
Cuts at: 33 240 576 1111 1260 1363 1497 1590 2275
Size: 207 336 535 149 103 134 93 685
Cuts at: 2275 3268 3459 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 535 336 207 191 149
134 103 93
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SgrAI Cr'CCGG_yG
Cuts at: 824 824
Size: 4547
SmaI CCC'GGG
Cuts at: 1569 1569
Size: 4547
SmlI C'TyrA_G
Cuts at: 1702 2617 3109 3371 1702
Size: 915 492 262 2878
Fragments arranged by size:
2878 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 1339 1339
Size: 4547
StyI C'CwwG_G
Cuts at: 1247 1681 2035 1247
Size: 434 354 3759
Fragments arranged by size:
3759 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 951 2130 2263 2722 2931 951
Size: 1179 133 459 209 2567
Fragments arranged by size:
2567 1179 459 209 133
TatI w'GTAC_w
Cuts at: 371 4062 371
Size: 3691 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 321 2042 321
Size: 1721 2826
XmaI C'CCGG_G
Cuts at: 1567 1567
Size: 4547
ZraI GAC'GTC
Cuts at: 300 300
Size: 4547
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BfrBI BmgBI BsaXI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SnaBI SrfI SspI SwaI XhoI XmnI