Restriction map for
pF1KB0462
(Circular) (Six-base) MAPSORT of: pF1KB0462.seq Check: 4006 from: 1 to: 6041
pF1KB0462 6041 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1787 1896 2562 1787
Size: 109 666 5266
Fragments arranged by size:
5266 666 109
AatII G_ACGT'C
Cuts at: 137 137
Size: 6041
AccI GT'mk_AC
Cuts at: 3079 3079
Size: 6041
Acc65I G'GTAC_C
Cuts at: 3057 3057
Size: 6041
AclI AA'CG_TT
Cuts at: 5881 5881
Size: 6041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 1655 2045 2227 2770 3866 4298 5045 5313
Size: 205 390 182 543 1096 432 747 268
Cuts at: 5313 1450
Size: 2178
Fragments arranged by size:
2178 1096 747 543 432 390 268 205
182
AfeI AGC'GCT
Cuts at: 127 239 127
Size: 112 5929
AflIII A'CryG_T
Cuts at: 2717 3393 4497 5474 2717
Size: 676 1104 977 3284
Fragments arranged by size:
3284 1104 977 676
AgeI A'CCGG_T
Cuts at: 1090 4429 1090
Size: 3339 2702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1047 2826 1047
Size: 1779 4262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 847
Size: 32 6009
AlwNI CAG_nnn'CTG
Cuts at: 368 708 933 1275 2445 3346 4913 368
Size: 340 225 342 1170 901 1567 1496
Fragments arranged by size:
1567 1496 1170 901 342 340 225
ApaI G_GGCC'C
Cuts at: 476 476
Size: 6041
ApaLI G'TGCA_C
Cuts at: 277 4811 277
Size: 4534 1507
ApoI r'AATT_y
Cuts at: 1493 2883 3045 5394 5650 1493
Size: 1390 162 2349 256 1884
Fragments arranged by size:
2349 1884 1390 256 162
AseI AT'TA_AT
Cuts at: 20 20
Size: 6041
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6041
AvaI C'yCGr_G
Cuts at: 1849 2122 2545 2756 3018 3061 1849
Size: 273 423 211 262 43 4829
Fragments arranged by size:
4829 423 273 262 211 43
BamHI G'GATC_C
Cuts at: 486 1436 3066 486
Size: 950 1630 3461
Fragments arranged by size:
3461 1630 950
BanI G'GyrC_C
Cuts at: 651 918 1093 1775 1968 2826 3057 3471 3719
Size: 267 175 682 193 858 231 414 248
Cuts at: 3719 3754 651
Size: 35 2938
Fragments arranged by size:
2938 858 682 414 267 248 231 193
175 35
BanII G_rGCy'C
Cuts at: 476 1013 1082 1961 2153 3055 4085 476
Size: 537 69 879 192 902 1030 2432
Fragments arranged by size:
2432 1030 902 879 537 192 69
BbeI G_GCGC'C
Cuts at: 3475 3723 3475
Size: 248 5793
BbsI GAAGACnn'nnnn_
Cuts at: 1643 2900 1643
Size: 1257 4784
BbvCI CC'TCA_GC
Cuts at: 1675 1675
Size: 6041
BciVI GTATCCnnnnn_n'
Cuts at: 1468 3939 4700 6041 1468
Size: 2471 761 1341 1468
Fragments arranged by size:
2471 1468 1341 761
BclI T'GATC_A
Cuts at: 2381 3561 2381
Size: 1180 4861
BglI GCCn_nnn'nGGC
Cuts at: 262 763 902 1134 1143 262
Size: 501 139 232 9 5160
Fragments arranged by size:
5160 501 232 139 9
BglII A'GATC_T
Cuts at: 2957 3556 5593 2957
Size: 599 2037 3405
Fragments arranged by size:
3405 2037 599
BlpI GC'TnA_GC
Cuts at: 234 383 3153 234
Size: 149 2770 3122
Fragments arranged by size:
3122 2770 149
Bme1580I G_kGCm'C
Cuts at: 281 307 476 1098 1331 1778 3666 3759 4815
Size: 26 169 622 233 447 1888 93 1056
Cuts at: 4815 281
Size: 1507
Fragments arranged by size:
1888 1507 1056 622 447 233 169 93
26
BmgBI CAC'GTC
Cuts at: 1884 1884
Size: 6041
BmrI ACTGGGnnnn_n'
Cuts at: 1142 3253 3334 3669 5813 1142
Size: 2111 81 335 2144 1370
Fragments arranged by size:
2144 2111 1370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1578 4408 1578
Size: 2830 3211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 1555 2104 2467 2758 1462
Size: 93 549 363 291 4745
Fragments arranged by size:
4745 549 363 291 93
Bpu10I CC'TnA_GC
Cuts at: 676 1675 676
Size: 999 5042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 835 997 3217 4096 4588 4886 787
Size: 48 162 2220 879 492 298 1942
Fragments arranged by size:
2220 1942 879 492 298 162 48
BsaI GGTCTCn'nnnn_
Cuts at: 1578 2877 5745 1578
Size: 1299 2868 1874
Fragments arranged by size:
2868 1874 1299
BsaAI yAC'GTr
Cuts at: 2720 3394 2720
Size: 674 5367
BsaBI GATnn'nnATC
Cuts at: 6 2257 3555 5592 6
Size: 2251 1298 2037 455
Fragments arranged by size:
2251 2037 1298 455
BsaHI Gr'CG_yC
Cuts at: 134 1220 2534 2666 3472 3720 4422 5918 134
Size: 1086 1314 132 806 248 702 1496 257
Fragments arranged by size:
1496 1314 1086 806 702 257 248 132
BsaWI w'CCGG_w
Cuts at: 489 1090 1334 2072 2705 3230 3751 4429 4703
Size: 601 244 738 633 525 521 678 274
Cuts at: 4703 4850 5261 489
Size: 147 411 1269
Fragments arranged by size:
1269 738 678 633 601 525 521 411
274 244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 847 877 1990 2020 2359 2389 334
Size: 30 483 30 1113 30 339 30 3986
Fragments arranged by size:
3986 1113 483 339 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 942 945 1459 1867 2014 2883 2892 616
Size: 326 3 514 408 147 869 9 3765
Fragments arranged by size:
3765 869 514 408 326 147 9 3
BseYI C'CCAG_C
Cuts at: 117 404 1045 1600 1665 3466 4224 4801 5297
Size: 287 641 555 65 1801 758 577 496
Cuts at: 5297 117
Size: 861
Fragments arranged by size:
1801 861 758 641 577 555 496 287
65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 721 1288 1392 3031 535
Size: 186 567 104 1639 3545
Fragments arranged by size:
3545 1639 567 186 104
BsiEI CG_ry'CG
Cuts at: 104 162 2626 3583 3629 4837 104
Size: 58 2464 957 46 1208 1308
Fragments arranged by size:
2464 1308 1208 957 58 46
BsiHKAI G_wGCw'C
Cuts at: 235 281 437 456 503 1013 3055 3833 4023
Size: 46 156 19 47 510 2042 778 190
Cuts at: 4023 4815 235
Size: 792 1461
Fragments arranged by size:
2042 1461 792 778 510 190 156 47
46 19
BsiWI C'GTAC_G
Cuts at: 2720 2720
Size: 6041
BsmI GAATG_Cn'
Cuts at: 336 1178 1611 2846 336
Size: 842 433 1235 3531
Fragments arranged by size:
3531 1235 842 433
BsmBI CGTCTCn'nnnn_
Cuts at: 420 2895 420
Size: 2475 3566
Bsp1286I G_dGCh'C
Cuts at: 235 281 307 437 456 476 503 1013 1082
Size: 46 26 130 19 20 27 510 69
Cuts at: 1082 1098 1331 1778 1961 2153 3055 3666 3759
Size: 16 233 447 183 192 902 611 93
Cuts at: 3759 3833 4023 4085 4815 235
Size: 74 190 62 730 1461
Fragments arranged by size:
1461 902 730 611 510 447 233 192
190 183 130 93 74 69 62 46
27 26 20 19 16
BspEI T'CCGG_A
Cuts at: 489 2072 2705 5261 489
Size: 1583 633 2556 1269
Fragments arranged by size:
2556 1583 1269 633
BspHI T'CATG_A
Cuts at: 5217 5217
Size: 6041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 1787 1896 2271 2562 3091 3305 3607 3988
Size: 846 109 375 291 529 214 302 381
Cuts at: 3988 941
Size: 2994
Fragments arranged by size:
2994 846 529 381 375 302 291 214
109
BsrBI CCG'CTC
Cuts at: 50 4333 4387 5871 6036 50
Size: 4283 54 1484 165 55
Fragments arranged by size:
4283 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2820 2977 3953 2820
Size: 157 976 4908
Fragments arranged by size:
4908 976 157
BsrFI r'CCGG_y
Cuts at: 1090 2621 2625 2732 2998 4039 4220 4429 1090
Size: 1531 4 107 266 1041 181 209 2702
Fragments arranged by size:
2702 1531 1041 266 209 181 107 4
BsrGI T'GTAC_A
Cuts at: 974 2981 974
Size: 2007 4034
BssSI C'ACGA_G
Cuts at: 208 2965 4312 4670 208
Size: 2757 1347 358 1579
Fragments arranged by size:
2757 1579 1347 358
BstAPI GCAn_nnn'nTGC
Cuts at: 708 3418 708
Size: 2710 3331
BstBI TT'CG_AA
Cuts at: 4402 4402
Size: 6041
BstEII G'GTnAC_C
Cuts at: 1719 1719
Size: 6041
BstXI CCAn_nnnn'nTGG
Cuts at: 1053 1533 1053
Size: 480 5561
BstYI r'GATC_y
Cuts at: 1 486 1271 1388 1436 1701 2744 2957 3066
Size: 485 785 117 48 265 1043 213 109
Cuts at: 3066 3533 3556 3891 4137 5149 5235 5247 5593
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5593 1
Size: 449
Fragments arranged by size:
1043 1012 785 485 467 449 346 335
265 246 213 117 109 86 48 23
12
Bsu36I CC'TnA_GG
Cuts at: 361 361
Size: 6041
BtgI C'CryG_G
Cuts at: 107 478 959 1862 1875 2669 107
Size: 371 481 903 13 794 3479
Fragments arranged by size:
3479 903 794 481 371 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2244 3991 4172 4335 2244
Size: 1747 181 163 3950
Fragments arranged by size:
3950 1747 181 163
BtsI GCAGTG_nn'
Cuts at: 655 3284 655
Size: 2629 3412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1572 1607 1572
Size: 35 6006
DraI TTT'AAA
Cuts at: 3040 3040
Size: 6041
DrdI GACnn_nn'nnGTC
Cuts at: 1318 3747 4605 1318
Size: 2429 858 2754
Fragments arranged by size:
2754 2429 858
EaeI y'GGCC_r
Cuts at: 695 764 903 962 2623 2948 2974 3626 3800
Size: 69 139 59 1661 325 26 652 174
Cuts at: 3800 4191 4218 5563 695
Size: 391 27 1345 1173
Fragments arranged by size:
1661 1345 1173 652 391 325 174 139
69 59 27 26
EagI C'GGCC_G
Cuts at: 2623 3626 2623
Size: 1003 5038
EarI CTCTTCn'nnn_
Cuts at: 1189 1924 2203 2590 2901 2919 4064 4274 1189
Size: 735 279 387 311 18 1145 210 2956
Fragments arranged by size:
2956 1145 735 387 311 279 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4249 4559 4705 5851 4249
Size: 310 146 1146 4439
Fragments arranged by size:
4439 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1011 3053 1011
Size: 2042 3999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 1462 1555 1655 2045 2104 2227 2467 2758
Size: 12 93 100 390 59 123 240 291
Cuts at: 2758 2770 3866 4298 5045 5313 1450
Size: 12 1096 432 747 268 2178
Fragments arranged by size:
2178 1096 747 432 390 291 268 240
123 100 93 59 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 666 729 2501 666
Size: 63 1772 4206
Fragments arranged by size:
4206 1772 63
EcoO109I rG'GnC_Cy
Cuts at: 472 473 630 756 840 909 930 2184 2289
Size: 1 157 126 84 69 21 1254 105
Cuts at: 2289 2343 2607 3022 3180 472
Size: 54 264 415 158 3333
Fragments arranged by size:
3333 1254 415 264 158 157 126 105
84 69 54 21 1
EcoRI G'AATT_C
Cuts at: 3045 3045
Size: 6041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1410 2601 4060 1410
Size: 1191 1459 3391
Fragments arranged by size:
3391 1459 1191
FspI TGC'GCA
Cuts at: 3822 3822
Size: 6041
HaeII r_GCGC'y
Cuts at: 129 241 293 988 1404 1452 3475 3723 4745
Size: 112 52 695 416 48 2023 248 1022
Cuts at: 4745 5724 129
Size: 979 446
Fragments arranged by size:
2023 1022 979 695 446 416 248 112
52 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 419 451 2750 2782 3883 3913 3915
Size: 32 53 32 2299 32 1101 30 2
Cuts at: 3915 3945 334
Size: 30 2430
Fragments arranged by size:
2430 2299 1101 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3080 3080
Size: 6041
KasI G'GCGC_C
Cuts at: 3471 3719 3471
Size: 248 5793
KpnI G_GTAC'C
Cuts at: 3061 3061
Size: 6041
MluI A'CGCG_T
Cuts at: 5474 5474
Size: 6041
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1133 2817 4712 4896 1133
Size: 1684 1895 184 2278
Fragments arranged by size:
2278 1895 1684 184
MscI TGG'CCA
Cuts at: 766 905 964 2976 3802 766
Size: 139 59 2012 826 3005
Fragments arranged by size:
3005 2012 826 139 59
MslI CAynn'nnrTG
Cuts at: 4030 4157 4030
Size: 127 5914
MspA1I CmG'CkG
Cuts at: 164 519 3152 3466 3826 4839 5084 164
Size: 355 2633 314 360 1013 245 1121
Fragments arranged by size:
2633 1121 1013 360 355 314 245
NaeI GCC'GGC
Cuts at: 3000 4222 3000
Size: 1222 4819
NarI GG'CG_CC
Cuts at: 3472 3720 3472
Size: 248 5793
NcoI C'CATG_G
Cuts at: 107 959 107
Size: 852 5189
NgoMIV G'CCGG_C
Cuts at: 2998 4220 2998
Size: 1222 4819
NspI r_CATG'y
Cuts at: 1632 2767 3094 3594 4501 1632
Size: 1135 327 500 907 3172
Fragments arranged by size:
3172 1135 907 500 327
PasI CC'CwG_GG
Cuts at: 633 1302 2610 633
Size: 669 1308 4064
Fragments arranged by size:
4064 1308 669
PciI A'CATG_T
Cuts at: 4497 4497
Size: 6041
PflMI CCAn_nnn'nTGG
Cuts at: 2445 2736 2445
Size: 291 5750
PfoI T'CCnGG_A
Cuts at: 770 1256 1379 1439 2285 2651 770
Size: 486 123 60 846 366 4160
Fragments arranged by size:
4160 846 486 366 123 60
PmeI GTTT'AAAC
Cuts at: 3040 3040
Size: 6041
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 847 879 5180 5212 847
Size: 32 4301 32 1676
Fragments arranged by size:
4301 1676 32 32
PpuMI rG'GwC_Cy
Cuts at: 930 2184 2289 2343 3022 930
Size: 1254 105 54 679 3949
Fragments arranged by size:
3949 1254 679 105 54
PshAI GACnn'nnGTC
Cuts at: 1826 1826
Size: 6041
PsiI TTA'TAA
Cuts at: 2642 2642
Size: 6041
PspOMI G'GGCC_C
Cuts at: 472 472
Size: 6041
PstI C_TGCA'G
Cuts at: 1569 3088 3773 1569
Size: 1519 685 3837
Fragments arranged by size:
3837 1519 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6041
PvuII CAG'CTG
Cuts at: 3466 3826 3466
Size: 360 5681
RsrII CG'GwC_CG
Cuts at: 4236 4236
Size: 6041
SacI G_AGCT'C
Cuts at: 1013 3055 1013
Size: 2042 3999
SalI G'TCGA_C
Cuts at: 3078 3078
Size: 6041
SanDI GG'GwC_CC
Cuts at: 2184 2184
Size: 6041
SapI GCTCTTCn'nnn_
Cuts at: 1924 4064 4274 1924
Size: 2140 210 3691
Fragments arranged by size:
3691 2140 210
SbfI CC_TGCA'GG
Cuts at: 3088 3088
Size: 6041
ScaI AGT'ACT
Cuts at: 5558 5558
Size: 6041
SfcI C'TryA_G
Cuts at: 33 346 1565 3084 3769 4762 4953 33
Size: 313 1219 1519 685 993 191 1121
Fragments arranged by size:
1519 1219 1121 993 685 313 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 763 902 1143 763
Size: 139 241 5661
Fragments arranged by size:
5661 241 139
SfoI GGC'GCC
Cuts at: 3473 3721 3473
Size: 248 5793
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6041
SgrAI Cr'CCGG_yG
Cuts at: 1090 1090
Size: 6041
SmaI CCC'GGG
Cuts at: 3020 3063 3020
Size: 43 5998
SmlI C'TyrA_G
Cuts at: 802 814 1012 3196 4111 4603 4865 802
Size: 12 198 2184 915 492 262 1978
Fragments arranged by size:
2184 1978 915 492 262 198 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6041
SphI G_CATG'C
Cuts at: 3094 3594 3094
Size: 500 5541
StuI AGG'CCT
Cuts at: 719 2496 719
Size: 1777 4264
StyI C'CwwG_G
Cuts at: 107 752 875 914 959 1082 1937 2318 2502
Size: 645 123 39 45 123 855 381 184
Cuts at: 2502 2822 3175 3529 107
Size: 320 353 354 2619
Fragments arranged by size:
2619 855 645 381 354 353 320 184
123 123 45 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 539 1601 3624 3757 4216 4425 539
Size: 1062 2023 133 459 209 2155
Fragments arranged by size:
2155 2023 1062 459 209 133
TatI w'GTAC_w
Cuts at: 974 2981 5556 974
Size: 2007 2575 1459
Fragments arranged by size:
2575 2007 1459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5558 5590 5558
Size: 32 6009
Tth111I GACn'n_nGTC
Cuts at: 3838 3838
Size: 6041
XbaI T'CTAG_A
Cuts at: 3072 3072
Size: 6041
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 759 3536 114
Size: 645 2777 2619
Fragments arranged by size:
2777 2619 645
XmaI C'CCGG_G
Cuts at: 3018 3061 3018
Size: 43 5998
ZraI GAC'GTC
Cuts at: 135 135
Size: 6041
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI AvrII BaeI BcgI BfrBI
BmtI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PspXI PsrI SacII SexAI SnaBI SrfI
SspI SwaI XhoI XmnI