Restriction map for
pF1KB0457
(Circular) (Six-base) MAPSORT of: pF1KB0457.seq Check: 9563 from: 1 to: 4628
pF1KB0457 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1546 1546
Size: 4628
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 995 1644 995
Size: 649 3979
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 760 2453 2885 3632 3900 126
Size: 634 1693 432 747 268 854
Fragments arranged by size:
1693 854 747 634 432 268
AfeI AGC'GCT
Cuts at: 1470 1470
Size: 4628
AflIII A'CryG_T
Cuts at: 1980 3084 4061 1980
Size: 1104 977 2547
Fragments arranged by size:
2547 1104 977
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AleI CACnn'nnGTG
Cuts at: 346 880 346
Size: 534 4094
AlwNI CAG_nnn'CTG
Cuts at: 1101 1453 1463 1610 1933 3500 1101
Size: 352 10 147 323 1567 2229
Fragments arranged by size:
2229 1567 352 323 147 10
ApaI G_GGCC'C
Cuts at: 326 326
Size: 4628
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 1632 3981 4237 1632
Size: 2349 256 2023
Fragments arranged by size:
2349 2023 256
AscI GG'CGCG_CC
Cuts at: 135 135
Size: 4628
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1400 1648 1400
Size: 248 4380
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 995 1225 1328 1644 2058 2306 2341 995
Size: 230 103 316 414 248 35 3282
Fragments arranged by size:
3282 414 316 248 230 103 35
BanII G_rGCy'C
Cuts at: 326 566 1567 1642 2672 326
Size: 240 1001 75 1030 2282
Fragments arranged by size:
2282 1030 1001 240 75
BbeI G_GCGC'C
Cuts at: 1229 1332 2062 2310 1229
Size: 103 730 248 3547
Fragments arranged by size:
3547 730 248 103
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 1031 1262 2148 1031
Size: 231 886 3511
Fragments arranged by size:
3511 886 231
BfrBI ATG'CAT
Cuts at: 338 338
Size: 4628
BglI GCCn_nnn'nGGC
Cuts at: 1156 1156
Size: 4628
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 326 2253 2346 3402 326
Size: 1927 93 1056 1552
Fragments arranged by size:
1927 1552 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 320 1155 1840 1921 2256 4400 320
Size: 835 685 81 335 2144 548
Fragments arranged by size:
2144 835 685 548 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 508 529 580 688 802 841 1165 1240
Size: 102 21 51 108 114 39 324 75
Cuts at: 1240 1357 406
Size: 117 3677
Fragments arranged by size:
3677 324 117 114 108 102 75 51
39 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2683 3175 3473 1804
Size: 879 492 298 2959
Fragments arranged by size:
2959 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1039 4332 1039
Size: 3293 1335
BsaAI yAC'GTr
Cuts at: 654 1981 654
Size: 1327 3301
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 1226 1329 2059 2307 3009 4505 1226
Size: 103 730 248 702 1496 1349
Fragments arranged by size:
1496 1349 730 702 248 103
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 592 622 553
Size: 30 9 30 4559
Fragments arranged by size:
4559 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 436 706 709 853 856 1027 1081 1360
Size: 78 270 3 144 3 171 54 279
Cuts at: 1360 358
Size: 3626
Fragments arranged by size:
3626 279 270 171 144 78 54 3
3
BseYI C'CCAG_C
Cuts at: 1296 2053 2811 3388 3884 1296
Size: 757 758 577 496 2040
Fragments arranged by size:
2040 758 757 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 676
Size: 4628
BsiEI CG_ry'CG
Cuts at: 104 177 1109 2170 2216 3424 104
Size: 73 932 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 932 73 46
BsiHKAI G_wGCw'C
Cuts at: 1501 1642 2420 2610 3402 1501
Size: 141 778 190 792 2727
Fragments arranged by size:
2727 792 778 190 141
BsmI GAATG_Cn'
Cuts at: 851 1125 851
Size: 274 4354
Bsp1286I G_dGCh'C
Cuts at: 326 566 1501 1567 1642 2253 2346 2420 2610
Size: 240 935 66 75 611 93 74 190
Cuts at: 2610 2672 3402 326
Size: 62 730 1552
Fragments arranged by size:
1552 935 730 611 240 190 93 75
74 66 62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 121 443 1546 1678 1892 2194 2575 121
Size: 322 1103 132 214 302 381 2174
Fragments arranged by size:
2174 1103 381 322 302 214 132
BsrBI CCG'CTC
Cuts at: 50 305 2920 2974 4458 4623 50
Size: 255 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 255 165 55 54
BsrDI GCAATG_nn'
Cuts at: 929 2540 929
Size: 1611 3017
BsrFI r'CCGG_y
Cuts at: 274 1501 2626 2807 3016 274
Size: 1227 1125 181 209 1886
Fragments arranged by size:
1886 1227 1125 209 181
BssHII G'CGCG_C
Cuts at: 135 135
Size: 4628
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 215 663 1463 1610 2005 215
Size: 448 800 147 395 2838
Fragments arranged by size:
2838 800 448 395 147
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstYI r'GATC_y
Cuts at: 1 845 1653 2120 2143 2478 2724 3736 3822
Size: 844 808 467 23 335 246 1012 86
Cuts at: 3822 3834 4180 1
Size: 12 346 449
Fragments arranged by size:
1012 844 808 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 823 823
Size: 4628
BtgI C'CryG_G
Cuts at: 166 204 222 881 1025 1151 166
Size: 38 18 659 144 126 3643
Fragments arranged by size:
3643 659 144 126 38 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 412 1464 1871 412
Size: 1052 407 3169
Fragments arranged by size:
3169 1052 407
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DraIII CAC_nnn'GTG
Cuts at: 255 255
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 961 2334 3192 961
Size: 1373 858 2397
Fragments arranged by size:
2397 1373 858
EaeI y'GGCC_r
Cuts at: 174 1106 1211 1444 1521 2213 2387 2778 2805
Size: 932 105 233 77 692 174 391 27
Cuts at: 2805 4150 174
Size: 1345 652
Fragments arranged by size:
1345 932 692 652 391 233 174 105
77 27
EagI C'GGCC_G
Cuts at: 174 1106 2213 174
Size: 932 1107 2589
Fragments arranged by size:
2589 1107 932
EarI CTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 406 508 529 580 688 760 802 841
Size: 280 102 21 51 108 72 42 39
Cuts at: 841 1165 1240 1357 2453 2885 3632 3900 126
Size: 324 75 117 1096 432 747 268 854
Fragments arranged by size:
1096 854 747 432 324 280 268 117
108 102 75 72 51 42 39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 571 805 1234 1336 571
Size: 234 429 102 3863
Fragments arranged by size:
3863 429 234 102
EcoO109I rG'GnC_Cy
Cuts at: 798 909 1422 1767 798
Size: 111 513 345 3659
Fragments arranged by size:
3659 513 345 111
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 2647 498
Size: 2149 2479
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 1229 1332 1472 2062 2310 3332 4311 1229
Size: 103 140 590 248 1022 979 1546
Fragments arranged by size:
1546 1022 979 590 248 140 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 2470 2500 2502 2532 973
Size: 32 1465 30 2 30 3069
Fragments arranged by size:
3069 1465 32 30 30 2
HincII GTy'rAC
Cuts at: 357 1667 357
Size: 1310 3318
KasI G'GCGC_C
Cuts at: 1225 1328 2058 2306 1225
Size: 103 730 248 3547
Fragments arranged by size:
3547 730 248 103
KpnI G_GTAC'C
Cuts at: 999 1648 999
Size: 649 3979
MfeI C'AATT_G
Cuts at: 1525 1525
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3299 3483 3299
Size: 184 4444
MscI TGG'CCA
Cuts at: 2389 2389
Size: 4628
MslI CAynn'nnrTG
Cuts at: 346 880 2617 2744 346
Size: 534 1737 127 2230
Fragments arranged by size:
2230 1737 534 127
MspA1I CmG'CkG
Cuts at: 146 168 179 224 626 718 1153 1202 1610
Size: 22 11 45 402 92 435 49 408
Cuts at: 1610 1739 2053 2413 3426 3671 146
Size: 129 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 435 408 402 360 314 245
129 92 49 45 22 11
NaeI GCC'GGC
Cuts at: 276 2809 276
Size: 2533 2095
NarI GG'CG_CC
Cuts at: 1226 1329 2059 2307 1226
Size: 103 730 248 3547
Fragments arranged by size:
3547 730 248 103
NgoMIV G'CCGG_C
Cuts at: 274 2807 274
Size: 2533 2095
NsiI A_TGCA'T
Cuts at: 340 340
Size: 4628
NspI r_CATG'y
Cuts at: 1681 2181 3088 1681
Size: 500 907 3221
Fragments arranged by size:
3221 907 500
PasI CC'CwG_GG
Cuts at: 1584 1584
Size: 4628
PciI A'CATG_T
Cuts at: 3084 3084
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 1453 1453
Size: 4628
PfoI T'CCnGG_A
Cuts at: 485 806 485
Size: 321 4307
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PmlI CAC'GTG
Cuts at: 654 654
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 909 1422 909
Size: 513 4115
PspOMI G'GGCC_C
Cuts at: 322 322
Size: 4628
PstI C_TGCA'G
Cuts at: 524 1235 1468 1675 2360 524
Size: 711 233 207 685 2792
Fragments arranged by size:
2792 711 685 233 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 146 718 1610 2053 2413 146
Size: 572 892 443 360 2361
Fragments arranged by size:
2361 892 572 443 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SacII CC_GC'GG
Cuts at: 169 225 1154 169
Size: 56 929 3643
Fragments arranged by size:
3643 929 56
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 520 1231 1323 1464 1671 2356 3349 3540
Size: 487 711 92 141 207 685 993 191
Cuts at: 3540 33
Size: 1121
Fragments arranged by size:
1121 993 711 685 487 207 191 141
92
SfiI GGCCn_nnn'nGGCC
Cuts at: 1156 1156
Size: 4628
SfoI GGC'GCC
Cuts at: 1227 1330 2060 2308 1227
Size: 103 730 248 3547
Fragments arranged by size:
3547 730 248 103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1402 1650 1402
Size: 248 4380
SmlI C'TyrA_G
Cuts at: 1783 2698 3190 3452 1783
Size: 915 492 262 2959
Fragments arranged by size:
2959 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StyI C'CwwG_G
Cuts at: 1061 1762 2116 1061
Size: 701 354 3573
Fragments arranged by size:
3573 701 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 1576 2211 2344 2803 3012 342
Size: 1234 635 133 459 209 1958
Fragments arranged by size:
1958 1234 635 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 4145 4177 592
Size: 32 3521 32 1043
Fragments arranged by size:
3521 1043 32 32
Tth111I GACn'n_nGTC
Cuts at: 961 2425 961
Size: 1464 3164
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1248 1299 2123 1248
Size: 51 824 3753
Fragments arranged by size:
3753 824 51
XmaI C'CCGG_G
Cuts at: 1400 1648 1400
Size: 248 4380
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AvrII BaeI BbsI
BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI PacI
PshAI PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI