Restriction map for
pF1KB0455
(Circular) (Six-base) MAPSORT of: pF1KB0455.seq Check: 2542 from: 1 to: 4628
pF1KB0455 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1492 1492
Size: 4628
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 367 391 940 1099 1163 2453 2885 3632
Size: 162 24 549 159 64 1290 432 747
Cuts at: 3632 3900 205
Size: 268 933
Fragments arranged by size:
1290 933 747 549 432 268 162 159
64 24
AflIII A'CryG_T
Cuts at: 1124 1358 1980 3084 4061 1124
Size: 234 622 1104 977 1691
Fragments arranged by size:
1691 1104 977 622 234
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 4628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 539 539
Size: 4628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 463 495 921 953 463
Size: 32 426 32 4138
Fragments arranged by size:
4138 426 32 32
AlwNI CAG_nnn'CTG
Cuts at: 345 369 708 753 1933 3500 345
Size: 24 339 45 1180 1567 1473
Fragments arranged by size:
1567 1473 1180 339 45 24
ApaI G_GGCC'C
Cuts at: 798 798
Size: 4628
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 1170 1632 3981 4237 1170
Size: 462 2349 256 1561
Fragments arranged by size:
2349 1561 462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 449 847 1432 1492 1537 1648 449
Size: 398 585 60 45 111 3429
Fragments arranged by size:
3429 585 398 111 60 45
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 580 1407 1644 2058 2306 2341 580
Size: 827 237 414 248 35 2867
Fragments arranged by size:
2867 827 414 248 237 35
BanII G_rGCy'C
Cuts at: 181 798 1028 1642 2672 181
Size: 617 230 614 1030 2137
Fragments arranged by size:
2137 1030 617 614 230
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 228 287 1087 1332 228
Size: 59 800 245 3524
Fragments arranged by size:
3524 800 245 59
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 626 2148 626
Size: 1522 3106
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 434 798 2253 2346 3402 434
Size: 364 1455 93 1056 1660
Fragments arranged by size:
1660 1455 1056 364 93
BmgBI CAC'GTC
Cuts at: 1359 1359
Size: 4628
BmrI ACTGGGnnnn_n'
Cuts at: 428 1840 1921 2256 4400 428
Size: 1412 81 335 2144 656
Fragments arranged by size:
2144 1412 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 685 923 1471 1597 401
Size: 284 238 548 126 3432
Fragments arranged by size:
3432 548 284 238 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2683 3175 3473 1804
Size: 879 492 298 2959
Fragments arranged by size:
2959 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 209 1508 2059 2307 3009 4505 209
Size: 1299 551 248 702 1496 332
Fragments arranged by size:
1496 1299 702 551 332 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 921 951 658
Size: 30 233 30 4335
Fragments arranged by size:
4335 233 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 242 1555 242
Size: 1313 3315
BseYI C'CCAG_C
Cuts at: 2053 2811 3388 3884 2053
Size: 758 577 496 2797
Fragments arranged by size:
2797 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4628
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1642 2420 2610 3402 1642
Size: 778 190 792 2868
Fragments arranged by size:
2868 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 181 434 798 1028 1642 2253 2346 2420 2610
Size: 253 364 230 614 611 93 74 190
Cuts at: 2610 2672 3402 181
Size: 62 730 1407
Fragments arranged by size:
1407 730 614 611 364 253 230 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 1196 1492 1678 1892 2194 2575 344
Size: 852 296 186 214 302 381 2397
Fragments arranged by size:
2397 852 381 302 296 214 186
BsrBI CCG'CTC
Cuts at: 50 990 1545 1602 2920 2974 4458 4623 50
Size: 940 555 57 1318 54 1484 165 55
Fragments arranged by size:
1484 1318 940 555 165 57 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 541 1532 2626 2807 3016 541
Size: 991 1094 181 209 2153
Fragments arranged by size:
2153 1094 991 209 181
BssSI C'ACGA_G
Cuts at: 985 2899 3257 985
Size: 1914 358 2356
Fragments arranged by size:
2356 1914 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1572 2005 1572
Size: 433 4195
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstEII G'GTnAC_C
Cuts at: 1014 1014
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1206 114
Size: 1092 3536
BstYI r'GATC_y
Cuts at: 1 641 812 1653 2120 2143 2478 2724 3736
Size: 640 171 841 467 23 335 246 1012
Cuts at: 3736 3822 3834 4180 1
Size: 86 12 346 449
Fragments arranged by size:
1012 841 640 467 449 346 335 246
171 86 23 12
BtgI C'CryG_G
Cuts at: 107 1503 107
Size: 1396 3232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1497 2578 2759 2922 1497
Size: 1081 181 163 3203
Fragments arranged by size:
3203 1081 181 163
BtsI GCAGTG_nn'
Cuts at: 217 1016 1871 217
Size: 799 855 2974
Fragments arranged by size:
2974 855 799
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 395 905 1052 1196 1592 2213 2387 2778 2805
Size: 510 147 144 396 621 174 391 27
Cuts at: 2805 4150 395
Size: 1345 873
Fragments arranged by size:
1345 873 621 510 396 391 174 147
144 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 411 2651 2861 411
Size: 2240 210 2178
Fragments arranged by size:
2240 2178 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 367 391 401 685 923 940 1099 1163
Size: 162 24 10 284 238 17 159 64
Cuts at: 1163 1471 1597 2453 2885 3632 3900 205
Size: 308 126 856 432 747 268 933
Fragments arranged by size:
933 856 747 432 308 284 268 238
162 159 126 64 24 17 10
EcoNI CCTnn'n_nnAGG
Cuts at: 955 955
Size: 4628
EcoO109I rG'GnC_Cy
Cuts at: 333 366 705 1767 333
Size: 33 339 1062 3194
Fragments arranged by size:
3194 1062 339 33
EcoRI G'AATT_C
Cuts at: 1170 1632 1170
Size: 462 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 197 2062 2310 3332 4311 197
Size: 1865 248 1022 979 514
Fragments arranged by size:
1865 1022 979 514 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 960 992 1091 1123 1348 1380 2470 2500 2502
Size: 32 99 32 225 32 1090 30 2
Cuts at: 2502 2532 960
Size: 30 3056
Fragments arranged by size:
3056 1090 225 99 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 478 1023 3299 3483 478
Size: 545 2276 184 1623
Fragments arranged by size:
2276 1623 545 184
MscI TGG'CCA
Cuts at: 397 907 1054 2389 397
Size: 510 147 1335 2636
Fragments arranged by size:
2636 1335 510 147
MslI CAynn'nnrTG
Cuts at: 275 319 901 1207 2617 2744 275
Size: 44 582 306 1410 127 2159
Fragments arranged by size:
2159 1410 582 306 127 44
MspA1I CmG'CkG
Cuts at: 647 1505 1739 2053 2413 3426 3671 647
Size: 858 234 314 360 1013 245 1604
Fragments arranged by size:
1604 1013 858 360 314 245 234
NaeI GCC'GGC
Cuts at: 543 1534 2809 543
Size: 991 1275 2362
Fragments arranged by size:
2362 1275 991
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 107 107
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 541 1532 2807 541
Size: 991 1275 2362
Fragments arranged by size:
2362 1275 991
NspI r_CATG'y
Cuts at: 1128 1470 1681 2181 3088 1128
Size: 342 211 500 907 2668
Fragments arranged by size:
2668 907 500 342 211
PasI CC'CwG_GG
Cuts at: 615 791 1417 615
Size: 176 626 3826
Fragments arranged by size:
3826 626 176
PciI A'CATG_T
Cuts at: 1124 3084 1124
Size: 1960 2668
PflMI CCAn_nnn'nTGG
Cuts at: 708 1307 708
Size: 599 4029
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 921 953 3767 3799 921
Size: 32 2814 32 1750
Fragments arranged by size:
2814 1750 32 32
PpuMI rG'GwC_Cy
Cuts at: 333 366 333
Size: 33 4595
PspOMI G'GGCC_C
Cuts at: 794 794
Size: 4628
PstI C_TGCA'G
Cuts at: 341 983 1568 1619 1675 2360 341
Size: 642 585 51 56 685 2609
Fragments arranged by size:
2609 685 642 585 56 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 2053 2413 2053
Size: 360 4268
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SacII CC_GC'GG
Cuts at: 1506 1506
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 337 361 979 1564 1615 1671 2356 3349
Size: 304 24 618 585 51 56 685 993
Cuts at: 3349 3540 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 618 585 304 191 56
51 24
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 1783 2698 3190 3452 1783
Size: 915 492 262 2959
Fragments arranged by size:
2959 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1470 1681 2181 1470
Size: 211 500 3917
Fragments arranged by size:
3917 500 211
StuI AGG'CCT
Cuts at: 1219 1219
Size: 4628
StyI C'CwwG_G
Cuts at: 107 1019 1111 1762 2116 107
Size: 912 92 651 354 2619
Fragments arranged by size:
2619 912 651 354 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 628 943 2211 2344 2803 3012 628
Size: 315 1268 133 459 209 2244
Fragments arranged by size:
2244 1268 459 315 209 133
TatI w'GTAC_w
Cuts at: 1046 4143 1046
Size: 3097 1531
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 2123 441
Size: 1682 2946
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI