Restriction map for
pF1KB0453
(Circular) (Six-base) MAPSORT of: pF1KB0453.seq Check: 9533 from: 1 to: 4628
pF1KB0453 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 824 1666 824
Size: 842 3786
Acc65I G'GTAC_C
Cuts at: 1136 1644 1136
Size: 508 4120
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 2453 2885 3632 3900 700
Size: 1753 432 747 268 1428
Fragments arranged by size:
1753 1428 747 432 268
AflIII A'CryG_T
Cuts at: 266 1980 3084 4061 266
Size: 1714 1104 977 833
Fragments arranged by size:
1714 1104 977 833
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AleI CACnn'nnGTG
Cuts at: 316 316
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1212 1224 1933 3500 1212
Size: 12 709 1567 2340
Fragments arranged by size:
2340 1567 709 12
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 546 967 1632 3981 4237 546
Size: 421 665 2349 256 937
Fragments arranged by size:
2349 937 665 421 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 251 506 1048 1648 251
Size: 255 542 600 3231
Fragments arranged by size:
3231 600 542 255
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 1053 1136 1644 2058 2306 2341 1053
Size: 83 508 414 248 35 3340
Fragments arranged by size:
3340 508 414 248 83 35
BanII G_rGCy'C
Cuts at: 1642 2672 1642
Size: 1030 3598
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbvCI CC'TCA_GC
Cuts at: 1582 1582
Size: 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1248 1282 1248
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 1058 1599 2253 2346 3402 1058
Size: 541 654 93 1056 2284
Fragments arranged by size:
2284 1056 654 541 93
BmgBI CAC'GTC
Cuts at: 846 846
Size: 4628
BmrI ACTGGGnnnn_n'
Cuts at: 1840 1921 2256 4400 1840
Size: 81 335 2144 2068
Fragments arranged by size:
2144 2068 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1370 2995 1370
Size: 1625 3003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 994 1246 634
Size: 360 252 4016
Fragments arranged by size:
4016 360 252
Bpu10I CC'TnA_GC
Cuts at: 1582 1582
Size: 4628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1365 1558 1804 2683 3175 3473 1365
Size: 193 246 879 492 298 2520
Fragments arranged by size:
2520 879 492 298 246 193
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 267 1981 267
Size: 1714 2914
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 1539 2059 2307 3009 4505 1539
Size: 520 248 702 1496 1662
Fragments arranged by size:
1662 1496 702 520 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 478 528 409
Size: 69 50 4509
Fragments arranged by size:
4509 69 50
BseYI C'CCAG_C
Cuts at: 2053 2811 3388 3884 2053
Size: 758 577 496 2797
Fragments arranged by size:
2797 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 1237
Size: 4628
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 426 1642 2420 2610 3402 426
Size: 1216 778 190 792 1652
Fragments arranged by size:
1652 1216 792 778 190
BsmI GAATG_Cn'
Cuts at: 697 697
Size: 4628
Bsp1286I G_dGCh'C
Cuts at: 426 1058 1599 1642 2253 2346 2420 2610 2672
Size: 632 541 43 611 93 74 190 62
Cuts at: 2672 3402 426
Size: 730 1652
Fragments arranged by size:
1652 730 632 611 541 190 93 74
62 43
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 833 3804 833
Size: 2971 1657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1892 2194 2575 1678
Size: 214 302 381 3731
Fragments arranged by size:
3731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 596 1266 2540 596
Size: 670 1274 2684
Fragments arranged by size:
2684 1274 670
BsrFI r'CCGG_y
Cuts at: 872 1565 2626 2807 3016 872
Size: 693 1061 181 209 2484
Fragments arranged by size:
2484 1061 693 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 171 1525 2989 171
Size: 1354 1464 1810
Fragments arranged by size:
1810 1464 1354
BstXI CCAn_nnnn'nTGG
Cuts at: 612 612
Size: 4628
BstYI r'GATC_y
Cuts at: 1 407 741 1653 2120 2143 2478 2724 3736
Size: 406 334 912 467 23 335 246 1012
Cuts at: 3736 3822 3834 4180 1
Size: 86 12 346 449
Fragments arranged by size:
1012 912 467 449 406 346 335 334
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 1072 1871 1072
Size: 799 3829
ClaI AT'CG_AT
Cuts at: 1182 1182
Size: 4628
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 2213 2387 2778 2805 4150 2213
Size: 174 391 27 1345 2691
Fragments arranged by size:
2691 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 676 1015 1100 1139 2651 2861 676
Size: 339 85 39 1512 210 2443
Fragments arranged by size:
2443 1512 339 210 85 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2836 3146 3292 4438 2836
Size: 310 146 1146 3026
Fragments arranged by size:
3026 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 700 994 1246 2453 2885 3632 3900 634
Size: 66 294 252 1207 432 747 268 1362
Fragments arranged by size:
1362 1207 747 432 294 268 252 66
EcoO109I rG'GnC_Cy
Cuts at: 1209 1767 1209
Size: 558 4070
EcoRI G'AATT_C
Cuts at: 546 1632 546
Size: 1086 3542
EcoRV GAT'ATC
Cuts at: 1186 1186
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 2647 579
Size: 2068 2560
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 436 2062 2310 3332 4311 436
Size: 1626 248 1022 979 753
Fragments arranged by size:
1626 1022 979 753 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 770 802 1340 1372 2470 2500 2502 2532 770
Size: 32 538 32 1098 30 2 30 2866
Fragments arranged by size:
2866 1098 538 32 32 30 30 2
HincII GTy'rAC
Cuts at: 789 1667 789
Size: 878 3750
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1140 1648 1140
Size: 508 4120
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3299 3483 3299
Size: 184 4444
MscI TGG'CCA
Cuts at: 2389 2389
Size: 4628
MslI CAynn'nnrTG
Cuts at: 316 389 757 2617 2744 316
Size: 73 368 1860 127 2200
Fragments arranged by size:
2200 1860 368 127 73
MspA1I CmG'CkG
Cuts at: 1221 1739 2053 2413 3426 3671 1221
Size: 518 314 360 1013 245 2178
Fragments arranged by size:
2178 1013 518 360 314 245
NaeI GCC'GGC
Cuts at: 874 1567 2809 874
Size: 693 1242 2693
Fragments arranged by size:
2693 1242 693
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NgoMIV G'CCGG_C
Cuts at: 872 1565 2807 872
Size: 693 1242 2693
Fragments arranged by size:
2693 1242 693
NspI r_CATG'y
Cuts at: 1608 1681 2181 3088 1608
Size: 73 500 907 3148
Fragments arranged by size:
3148 907 500 73
PciI A'CATG_T
Cuts at: 3084 3084
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 1212 1212
Size: 4628
PfoI T'CCnGG_A
Cuts at: 797 797
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 1209 1209
Size: 4628
PstI C_TGCA'G
Cuts at: 1675 2360 1675
Size: 685 3943
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 2053 2413 2053
Size: 360 4268
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SexAI A'CCwGG_T
Cuts at: 1211 1211
Size: 4628
SfcI C'TryA_G
Cuts at: 33 1671 2356 3349 3540 33
Size: 1638 685 993 191 1121
Fragments arranged by size:
1638 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 253 508 1650 253
Size: 255 1142 3231
Fragments arranged by size:
3231 1142 255
SmlI C'TyrA_G
Cuts at: 1344 1573 1783 2698 3190 3452 1344
Size: 229 210 915 492 262 2520
Fragments arranged by size:
2520 915 492 262 229 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
SspI AAT'ATT
Cuts at: 609 609
Size: 4628
StyI C'CwwG_G
Cuts at: 1762 2116 1762
Size: 354 4274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1382 2211 2344 2803 3012 1382
Size: 829 133 459 209 2998
Fragments arranged by size:
2998 829 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 251 506 1648 251
Size: 255 1142 3231
Fragments arranged by size:
3231 1142 255
XmnI GAAnn'nnTTC
Cuts at: 550 550
Size: 4628
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI