Restriction map for
pF1KB0441
(Circular) (Six-base) MAPSORT of: pF1KB0441.seq Check: 9333 from: 1 to: 4445
pF1KB0441 4445 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 1219 1219
Size: 4445
AccI GT'mk_AC
Cuts at: 1483 1483
Size: 4445
Acc65I G'GTAC_C
Cuts at: 335 1461 335
Size: 1126 3319
AclI AA'CG_TT
Cuts at: 4285 4285
Size: 4445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 2270 2702 3449 3717 958
Size: 1312 432 747 268 1686
Fragments arranged by size:
1686 1312 747 432 268
AfeI AGC'GCT
Cuts at: 1173 1173
Size: 4445
AflIII A'CryG_T
Cuts at: 1797 2901 3878 1797
Size: 1104 977 2364
Fragments arranged by size:
2364 1104 977
AgeI A'CCGG_T
Cuts at: 2833 2833
Size: 4445
AhdI GACnn_n'nnGTC
Cuts at: 150 392 150
Size: 242 4203
AlwNI CAG_nnn'CTG
Cuts at: 640 1750 3317 640
Size: 1110 1567 1768
Fragments arranged by size:
1768 1567 1110
ApaI G_GGCC'C
Cuts at: 132 132
Size: 4445
ApaLI G'TGCA_C
Cuts at: 3215 3215
Size: 4445
ApoI r'AATT_y
Cuts at: 270 958 1449 3798 4054 270
Size: 688 491 2349 256 661
Fragments arranged by size:
2349 688 661 491 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
AvaI C'yCGr_G
Cuts at: 339 1465 339
Size: 1126 3319
AvrII C'CTAG_G
Cuts at: 300 300
Size: 4445
BamHI G'GATC_C
Cuts at: 1470 1470
Size: 4445
BanI G'GyrC_C
Cuts at: 335 828 1461 1875 2123 2158 335
Size: 493 633 414 248 35 2622
Fragments arranged by size:
2622 633 493 414 248 35
BanII G_rGCy'C
Cuts at: 132 408 1002 1459 2489 132
Size: 276 594 457 1030 2088
Fragments arranged by size:
2088 1030 594 457 276
BbeI G_GCGC'C
Cuts at: 1879 2127 1879
Size: 248 4197
BciVI GTATCCnnnnn_n'
Cuts at: 2343 3104 4445 2343
Size: 761 1341 2343
Fragments arranged by size:
2343 1341 761
BclI T'GATC_A
Cuts at: 698 1965 698
Size: 1267 3178
BglI GCCn_nnn'nGGC
Cuts at: 412 899 1375 412
Size: 487 476 3482
Fragments arranged by size:
3482 487 476
BglII A'GATC_T
Cuts at: 1960 3997 1960
Size: 2037 2408
BlpI GC'TnA_GC
Cuts at: 1557 1557
Size: 4445
Bme1580I G_kGCm'C
Cuts at: 132 833 2070 2163 3219 132
Size: 701 1237 93 1056 1358
Fragments arranged by size:
1358 1237 1056 701 93
BmgBI CAC'GTC
Cuts at: 596 596
Size: 4445
BmrI ACTGGGnnnn_n'
Cuts at: 1178 1657 1738 2073 4217 1178
Size: 479 81 335 2144 1406
Fragments arranged by size:
2144 1406 479 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 4445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 685 403
Size: 282 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 2500 2992 3290 1621
Size: 879 492 298 2776
Fragments arranged by size:
2776 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 379 4149 379
Size: 3770 675
BsaAI yAC'GTr
Cuts at: 1798 1798
Size: 4445
BsaBI GATnn'nnATC
Cuts at: 6 1959 3996 6
Size: 1953 2037 455
Fragments arranged by size:
2037 1953 455
BsaHI Gr'CG_yC
Cuts at: 1216 1876 2124 2826 4322 1216
Size: 660 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 660 248
BsaWI w'CCGG_w
Cuts at: 537 1634 2155 2833 3107 3254 3665 537
Size: 1097 521 678 274 147 411 1317
Fragments arranged by size:
1317 1097 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 451
Size: 30 4415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 1402 682
Size: 720 3725
BseYI C'CCAG_C
Cuts at: 437 1023 1197 1206 1870 2628 3205 3701 437
Size: 586 174 9 664 758 577 496 1181
Fragments arranged by size:
1181 758 664 586 577 496 174 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 1393
Size: 4445
BsiEI CG_ry'CG
Cuts at: 104 1370 1987 2033 3241 104
Size: 1266 617 46 1208 1308
Fragments arranged by size:
1308 1266 1208 617 46
BsiHKAI G_wGCw'C
Cuts at: 1459 2237 2427 3219 1459
Size: 778 190 792 2685
Fragments arranged by size:
2685 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 132 408 833 1002 1459 2070 2163 2237 2427
Size: 276 425 169 457 611 93 74 190
Cuts at: 2427 2489 3219 132
Size: 62 730 1358
Fragments arranged by size:
1358 730 611 457 425 276 190 169
93 74 62
BspEI T'CCGG_A
Cuts at: 537 3665 537
Size: 3128 1317
BspHI T'CATG_A
Cuts at: 566 3621 566
Size: 3055 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1495 1709 2011 2392 1495
Size: 214 302 381 3548
Fragments arranged by size:
3548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2737 2791 4275 4440 50
Size: 2687 54 1484 165 55
Fragments arranged by size:
2687 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2357 2357
Size: 4445
BsrFI r'CCGG_y
Cuts at: 233 2443 2624 2833 233
Size: 2210 181 209 1845
Fragments arranged by size:
2210 1845 209 181
BsrGI T'GTAC_A
Cuts at: 488 488
Size: 4445
BssSI C'ACGA_G
Cuts at: 2716 3074 2716
Size: 358 4087
BstAPI GCAn_nnn'nTGC
Cuts at: 481 1822 481
Size: 1341 3104
BstBI TT'CG_AA
Cuts at: 2806 2806
Size: 4445
BstEII G'GTnAC_C
Cuts at: 858 858
Size: 4445
BstXI CCAn_nnnn'nTGG
Cuts at: 437 437
Size: 4445
BstYI r'GATC_y
Cuts at: 1 1074 1292 1470 1937 1960 2295 2541 3553
Size: 1073 218 178 467 23 335 246 1012
Cuts at: 3553 3639 3651 3997 1
Size: 86 12 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 218
178 86 23 12
BtgI C'CryG_G
Cuts at: 510 558 1221 510
Size: 48 663 3734
Fragments arranged by size:
3734 663 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 687 2395 2576 2739 687
Size: 1708 181 163 2393
Fragments arranged by size:
2393 1708 181 163
BtsI GCAGTG_nn'
Cuts at: 491 846 1688 491
Size: 355 842 3248
Fragments arranged by size:
3248 842 355
DraI TTT'AAA
Cuts at: 963 1444 963
Size: 481 3964
DraIII CAC_nnn'GTG
Cuts at: 981 981
Size: 4445
DrdI GACnn_nn'nnGTC
Cuts at: 1309 2151 3009 1309
Size: 842 858 2745
Fragments arranged by size:
2745 858 842
EaeI y'GGCC_r
Cuts at: 986 1367 2030 2204 2595 2622 3967 986
Size: 381 663 174 391 27 1345 1464
Fragments arranged by size:
1464 1345 663 391 381 174 27
EagI C'GGCC_G
Cuts at: 1367 2030 1367
Size: 663 3782
EarI CTCTTCn'nnn_
Cuts at: 688 1417 2468 2678 688
Size: 729 1051 210 2455
Fragments arranged by size:
2455 1051 729 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 802 2653 2963 3109 4255 802
Size: 1851 310 146 1146 992
Fragments arranged by size:
1851 1146 992 310 146
EcoICRI GAG'CTC
Cuts at: 1457 1457
Size: 4445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 685 958 2270 2702 3449 3717 403
Size: 282 273 1312 432 747 268 1131
Fragments arranged by size:
1312 1131 747 432 282 273 268
EcoO109I rG'GnC_Cy
Cuts at: 128 297 1011 1202 1584 128
Size: 169 714 191 382 2989
Fragments arranged by size:
2989 714 382 191 169
EcoRI G'AATT_C
Cuts at: 1449 1449
Size: 4445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4445
FspI TGC'GCA
Cuts at: 2226 2226
Size: 4445
HaeII r_GCGC'y
Cuts at: 1175 1879 2127 3149 4128 1175
Size: 704 248 1022 979 1492
Fragments arranged by size:
1492 1022 979 704 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 378 410 2287 2317 2319 2349 136
Size: 32 210 32 1877 30 2 30 2232
Fragments arranged by size:
2232 1877 210 32 32 30 30 2
HincII GTy'rAC
Cuts at: 123 447 1484 123
Size: 324 1037 3084
Fragments arranged by size:
3084 1037 324
KasI G'GCGC_C
Cuts at: 1875 2123 1875
Size: 248 4197
KpnI G_GTAC'C
Cuts at: 339 1465 339
Size: 1126 3319
MluI A'CGCG_T
Cuts at: 3878 3878
Size: 4445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 3116 3300 225
Size: 2891 184 1370
Fragments arranged by size:
2891 1370 184
MscI TGG'CCA
Cuts at: 988 2206 988
Size: 1218 3227
MslI CAynn'nnrTG
Cuts at: 112 137 851 2434 2561 112
Size: 25 714 1583 127 1996
Fragments arranged by size:
1996 1583 714 127 25
MspA1I CmG'CkG
Cuts at: 663 716 806 1556 1870 2230 3243 3488 663
Size: 53 90 750 314 360 1013 245 1620
Fragments arranged by size:
1620 1013 750 360 314 245 90 53
NaeI GCC'GGC
Cuts at: 2626 2626
Size: 4445
NarI GG'CG_CC
Cuts at: 1876 2124 1876
Size: 248 4197
NcoI C'CATG_G
Cuts at: 510 558 510
Size: 48 4397
NgoMIV G'CCGG_C
Cuts at: 2624 2624
Size: 4445
NspI r_CATG'y
Cuts at: 1498 1998 2905 1498
Size: 500 907 3038
Fragments arranged by size:
3038 907 500
PciI A'CATG_T
Cuts at: 2901 2901
Size: 4445
PflMI CCAn_nnn'nTGG
Cuts at: 640 984 640
Size: 344 4101
PfoI T'CCnGG_A
Cuts at: 1382 1382
Size: 4445
PmeI GTTT'AAAC
Cuts at: 1444 1444
Size: 4445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4413
PpuMI rG'GwC_Cy
Cuts at: 1202 1202
Size: 4445
PspOMI G'GGCC_C
Cuts at: 128 128
Size: 4445
PstI C_TGCA'G
Cuts at: 413 997 1292 1492 2177 413
Size: 584 295 200 685 2681
Fragments arranged by size:
2681 685 584 295 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4445
PvuII CAG'CTG
Cuts at: 663 716 1870 2230 663
Size: 53 1154 360 2878
Fragments arranged by size:
2878 1154 360 53
RsrII CG'GwC_CG
Cuts at: 2640 2640
Size: 4445
SacI G_AGCT'C
Cuts at: 1459 1459
Size: 4445
SalI G'TCGA_C
Cuts at: 1482 1482
Size: 4445
SanDI GG'GwC_CC
Cuts at: 1202 1202
Size: 4445
SapI GCTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
SbfI CC_TGCA'GG
Cuts at: 413 1492 413
Size: 1079 3366
ScaI AGT'ACT
Cuts at: 3962 3962
Size: 4445
SexAI A'CCwGG_T
Cuts at: 977 977
Size: 4445
SfcI C'TryA_G
Cuts at: 33 409 912 993 1288 1488 2173 3166 3357
Size: 376 503 81 295 200 685 993 191
Cuts at: 3357 33
Size: 1121
Fragments arranged by size:
1121 993 685 503 376 295 200 191
81
SfoI GGC'GCC
Cuts at: 1877 2125 1877
Size: 248 4197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
SmaI CCC'GGG
Cuts at: 341 1467 341
Size: 1126 3319
SmlI C'TyrA_G
Cuts at: 1600 2515 3007 3269 1600
Size: 915 492 262 2776
Fragments arranged by size:
2776 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4445
SphI G_CATG'C
Cuts at: 1498 1998 1498
Size: 500 3945
StuI AGG'CCT
Cuts at: 415 415
Size: 4445
StyI C'CwwG_G
Cuts at: 300 510 558 1579 1933 300
Size: 210 48 1021 354 2812
Fragments arranged by size:
2812 1021 354 210 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2028 2161 2620 2829 2028
Size: 133 459 209 3644
Fragments arranged by size:
3644 459 209 133
TatI w'GTAC_w
Cuts at: 488 3960 488
Size: 3472 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 668 700 3962 3994 418
Size: 32 218 32 3262 32 869
Fragments arranged by size:
3262 869 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2242 2242
Size: 4445
XbaI T'CTAG_A
Cuts at: 1476 1476
Size: 4445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 352 556 1303 1940 352
Size: 204 747 637 2857
Fragments arranged by size:
2857 747 637 204
XmaI C'CCGG_G
Cuts at: 339 1465 339
Size: 1126 3319
XmnI GAAnn'nnTTC
Cuts at: 1330 1330
Size: 4445
ZraI GAC'GTC
Cuts at: 1217 1217
Size: 4445
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AlfI AloI AscI BaeI BbsI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI