Restriction map for
pF1KB0417
(Circular) (Six-base) MAPSORT of: pF1KB0417.seq Check: 2871 from: 1 to: 4526
pF1KB0417 4526 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 1564 1564
Size: 4526
Acc65I G'GTAC_C
Cuts at: 1542 1542
Size: 4526
AclI AA'CG_TT
Cuts at: 4366 4366
Size: 4526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1406 2351 2783 3530 3798 439
Size: 967 945 432 747 268 1167
Fragments arranged by size:
1167 967 945 747 432 268
AflIII A'CryG_T
Cuts at: 1878 2982 3959 1878
Size: 1104 977 2445
Fragments arranged by size:
2445 1104 977
AgeI A'CCGG_T
Cuts at: 2914 2914
Size: 4526
AlwNI CAG_nnn'CTG
Cuts at: 1251 1831 3398 1251
Size: 580 1567 2379
Fragments arranged by size:
2379 1567 580
ApaI G_GGCC'C
Cuts at: 1458 1458
Size: 4526
ApaLI G'TGCA_C
Cuts at: 3296 3296
Size: 4526
ApoI r'AATT_y
Cuts at: 114 874 1530 3879 4135 114
Size: 760 656 2349 256 505
Fragments arranged by size:
2349 760 656 505 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4526
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
AvaI C'yCGr_G
Cuts at: 970 1049 1546 970
Size: 79 497 3950
Fragments arranged by size:
3950 497 79
BamHI G'GATC_C
Cuts at: 715 1551 715
Size: 836 3690
BanI G'GyrC_C
Cuts at: 194 1542 1956 2204 2239 194
Size: 1348 414 248 35 2481
Fragments arranged by size:
2481 1348 414 248 35
BanII G_rGCy'C
Cuts at: 443 783 1458 1540 2570 443
Size: 340 675 82 1030 2399
Fragments arranged by size:
2399 1030 675 340 82
BbeI G_GCGC'C
Cuts at: 198 1960 2208 198
Size: 1762 248 2516
Fragments arranged by size:
2516 1762 248
BbsI GAAGACnn'nnnn_
Cuts at: 542 1418 542
Size: 876 3650
BciVI GTATCCnnnnn_n'
Cuts at: 2424 3185 4526 2424
Size: 761 1341 2424
Fragments arranged by size:
2424 1341 761
BclI T'GATC_A
Cuts at: 221 2046 221
Size: 1825 2701
BfrBI ATG'CAT
Cuts at: 659 659
Size: 4526
BglII A'GATC_T
Cuts at: 2041 4078 2041
Size: 2037 2489
BlpI GC'TnA_GC
Cuts at: 1638 1638
Size: 4526
Bme1580I G_kGCm'C
Cuts at: 269 510 1458 2151 2244 3300 269
Size: 241 948 693 93 1056 1495
Fragments arranged by size:
1495 1056 948 693 241 93
BmgBI CAC'GTC
Cuts at: 408 408
Size: 4526
BmrI ACTGGGnnnn_n'
Cuts at: 1414 1738 1819 2154 4298 1414
Size: 324 81 335 2144 1642
Fragments arranged by size:
2144 1642 335 324 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 4526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 253 334 767 796 1349 226
Size: 27 81 433 29 553 3403
Fragments arranged by size:
3403 553 433 81 29 27
Bpu10I CC'TnA_GC
Cuts at: 733 733
Size: 4526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1702 2581 3073 3371 1112
Size: 590 879 492 298 2267
Fragments arranged by size:
2267 879 590 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4230 4230
Size: 4526
BsaAI yAC'GTr
Cuts at: 1879 1879
Size: 4526
BsaBI GATnn'nnATC
Cuts at: 6 2040 4077 6
Size: 2034 2037 455
Fragments arranged by size:
2037 2034 455
BsaHI Gr'CG_yC
Cuts at: 195 1957 2205 2907 4403 195
Size: 1762 248 702 1496 318
Fragments arranged by size:
1762 1496 702 318 248
BsaWI w'CCGG_w
Cuts at: 1715 2236 2914 3188 3335 3746 1715
Size: 521 678 274 147 411 2495
Fragments arranged by size:
2495 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 915 945 1073 1103 915
Size: 30 128 30 4338
Fragments arranged by size:
4338 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 273 331 273
Size: 58 4468
BseYI C'CCAG_C
Cuts at: 1951 2709 3286 3782 1951
Size: 758 577 496 2695
Fragments arranged by size:
2695 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2068 2114 3322 104
Size: 1964 46 1208 1308
Fragments arranged by size:
1964 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 783 1540 2318 2508 3300 443
Size: 340 757 778 190 792 1669
Fragments arranged by size:
1669 792 778 757 340 190
BsmBI CGTCTCn'nnnn_
Cuts at: 588 588
Size: 4526
Bsp1286I G_dGCh'C
Cuts at: 269 443 510 783 1458 1540 2151 2244 2318
Size: 174 67 273 675 82 611 93 74
Cuts at: 2318 2508 2570 3300 269
Size: 190 62 730 1495
Fragments arranged by size:
1495 730 675 611 273 190 174 93
82 74 67 62
BspEI T'CCGG_A
Cuts at: 3746 3746
Size: 4526
BspHI T'CATG_A
Cuts at: 3702 3702
Size: 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 1576 1790 2092 2473 356
Size: 1220 214 302 381 2409
Fragments arranged by size:
2409 1220 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2818 2872 4356 4521 50
Size: 2768 54 1484 165 55
Fragments arranged by size:
2768 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 2438 824
Size: 1614 2912
BsrFI r'CCGG_y
Cuts at: 191 1061 2524 2705 2914 191
Size: 870 1463 181 209 1803
Fragments arranged by size:
1803 1463 870 209 181
BssSI C'ACGA_G
Cuts at: 2797 3155 2797
Size: 358 4168
BstAPI GCAn_nnn'nTGC
Cuts at: 471 1903 471
Size: 1432 3094
BstBI TT'CG_AA
Cuts at: 878 2887 878
Size: 2009 2517
BstXI CCAn_nnnn'nTGG
Cuts at: 954 1188 954
Size: 234 4292
BstYI r'GATC_y
Cuts at: 1 352 668 715 1364 1551 2018 2041 2376
Size: 351 316 47 649 187 467 23 335
Cuts at: 2376 2622 3634 3720 3732 4078 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 649 467 449 351 346 335 316
246 187 86 47 23 12
Bsu36I CC'TnA_GG
Cuts at: 979 1244 979
Size: 265 4261
BtgI C'CryG_G
Cuts at: 107 721 1092 107
Size: 614 371 3541
Fragments arranged by size:
3541 614 371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2476 2657 2820 2476
Size: 181 163 4182
Fragments arranged by size:
4182 181 163
BtsI GCAGTG_nn'
Cuts at: 1769 1769
Size: 4526
DraI TTT'AAA
Cuts at: 1525 1525
Size: 4526
DraIII CAC_nnn'GTG
Cuts at: 1408 1408
Size: 4526
DrdI GACnn_nn'nnGTC
Cuts at: 2232 3090 2232
Size: 858 3668
EaeI y'GGCC_r
Cuts at: 138 788 2111 2285 2676 2703 4048 138
Size: 650 1323 174 391 27 1345 616
Fragments arranged by size:
1345 1323 650 616 391 174 27
EagI C'GGCC_G
Cuts at: 2111 2111
Size: 4526
EarI CTCTTCn'nnn_
Cuts at: 158 403 558 2549 2759 158
Size: 245 155 1991 210 1925
Fragments arranged by size:
1991 1925 245 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 923 2734 3044 3190 4336 923
Size: 1811 310 146 1146 1113
Fragments arranged by size:
1811 1146 1113 310 146
EcoICRI GAG'CTC
Cuts at: 441 781 1538 441
Size: 340 757 3429
Fragments arranged by size:
3429 757 340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 253 334 439 767 796 1349 1406 2351
Size: 27 81 105 328 29 553 57 945
Cuts at: 2351 2783 3530 3798 226
Size: 432 747 268 954
Fragments arranged by size:
954 945 747 553 432 328 268 105
81 57 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 139 316 139
Size: 177 4349
EcoO109I rG'GnC_Cy
Cuts at: 974 1665 974
Size: 691 3835
EcoRI G'AATT_C
Cuts at: 1530 1530
Size: 4526
EcoRV GAT'ATC
Cuts at: 1335 1335
Size: 4526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1162 2545 1162
Size: 1383 3143
FspI TGC'GCA
Cuts at: 2307 2307
Size: 4526
HaeII r_GCGC'y
Cuts at: 198 1960 2208 3230 4209 198
Size: 1762 248 1022 979 515
Fragments arranged by size:
1762 1022 979 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 344 376 590 622 921 953 1267
Size: 32 99 32 214 32 299 32 314
Cuts at: 1267 1299 2368 2398 2400 2430 213
Size: 32 1069 30 2 30 2309
Fragments arranged by size:
2309 1069 314 299 214 99 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 281 1565 281
Size: 1284 3242
HindIII A'AGCT_T
Cuts at: 584 1144 584
Size: 560 3966
HpaI GTT'AAC
Cuts at: 281 281
Size: 4526
KasI G'GCGC_C
Cuts at: 194 1956 2204 194
Size: 1762 248 2516
Fragments arranged by size:
2516 1762 248
KpnI G_GTAC'C
Cuts at: 1546 1546
Size: 4526
MluI A'CGCG_T
Cuts at: 3959 3959
Size: 4526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 3197 3381 971
Size: 2226 184 2116
Fragments arranged by size:
2226 2116 184
MscI TGG'CCA
Cuts at: 140 790 2287 140
Size: 650 1497 2379
Fragments arranged by size:
2379 1497 650
MslI CAynn'nnrTG
Cuts at: 289 654 952 2515 2642 289
Size: 365 298 1563 127 2173
Fragments arranged by size:
2173 1563 365 298 127
MspA1I CmG'CkG
Cuts at: 1637 1951 2311 3324 3569 1637
Size: 314 360 1013 245 2594
Fragments arranged by size:
2594 1013 360 314 245
NaeI GCC'GGC
Cuts at: 193 2707 193
Size: 2514 2012
NarI GG'CG_CC
Cuts at: 195 1957 2205 195
Size: 1762 248 2516
Fragments arranged by size:
2516 1762 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4526
NgoMIV G'CCGG_C
Cuts at: 191 2705 191
Size: 2514 2012
NsiI A_TGCA'T
Cuts at: 661 661
Size: 4526
NspI r_CATG'y
Cuts at: 474 663 1579 2079 2986 474
Size: 189 916 500 907 2014
Fragments arranged by size:
2014 916 907 500 189
PciI A'CATG_T
Cuts at: 2982 2982
Size: 4526
PfoI T'CCnGG_A
Cuts at: 242 242
Size: 4526
PmeI GTTT'AAAC
Cuts at: 1525 1525
Size: 4526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4494
PpuMI rG'GwC_Cy
Cuts at: 974 974
Size: 4526
PsiI TTA'TAA
Cuts at: 761 761
Size: 4526
PspOMI G'GGCC_C
Cuts at: 1454 1454
Size: 4526
PstI C_TGCA'G
Cuts at: 367 1573 2258 367
Size: 1206 685 2635
Fragments arranged by size:
2635 1206 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4526
PvuII CAG'CTG
Cuts at: 1951 2311 1951
Size: 360 4166
RsrII CG'GwC_CG
Cuts at: 2721 2721
Size: 4526
SacI G_AGCT'C
Cuts at: 443 783 1540 443
Size: 340 757 3429
Fragments arranged by size:
3429 757 340
SalI G'TCGA_C
Cuts at: 1563 1563
Size: 4526
SanDI GG'GwC_CC
Cuts at: 974 974
Size: 4526
SapI GCTCTTCn'nnn_
Cuts at: 2549 2759 2549
Size: 210 4316
SbfI CC_TGCA'GG
Cuts at: 1573 1573
Size: 4526
ScaI AGT'ACT
Cuts at: 961 4043 961
Size: 3082 1444
SfcI C'TryA_G
Cuts at: 33 363 1226 1569 2254 3247 3438 33
Size: 330 863 343 685 993 191 1121
Fragments arranged by size:
1121 993 863 685 343 330 191
SfoI GGC'GCC
Cuts at: 196 1958 2206 196
Size: 1762 248 2516
Fragments arranged by size:
2516 1762 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4526
SmaI CCC'GGG
Cuts at: 1548 1548
Size: 4526
SmlI C'TyrA_G
Cuts at: 1127 1681 2596 3088 3350 1127
Size: 554 915 492 262 2303
Fragments arranged by size:
2303 915 554 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4526
SphI G_CATG'C
Cuts at: 474 663 1579 2079 474
Size: 189 916 500 2921
Fragments arranged by size:
2921 916 500 189
StuI AGG'CCT
Cuts at: 1055 1055
Size: 4526
StyI C'CwwG_G
Cuts at: 107 135 249 492 1660 2014 107
Size: 28 114 243 1168 354 2619
Fragments arranged by size:
2619 1168 354 243 114 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 498 1396 2109 2242 2701 2910 498
Size: 898 713 133 459 209 2114
Fragments arranged by size:
2114 898 713 459 209 133
TatI w'GTAC_w
Cuts at: 959 4041 959
Size: 3082 1444
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 408 440 1073 1105 4043 4075 408
Size: 32 633 32 2938 32 859
Fragments arranged by size:
2938 859 633 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1216 2323 1216
Size: 1107 3419
XbaI T'CTAG_A
Cuts at: 1557 1557
Size: 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 2021
Size: 4526
XmaI C'CCGG_G
Cuts at: 1546 1546
Size: 4526
XmnI GAAnn'nnTTC
Cuts at: 298 874 298
Size: 576 3950
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
FseI FspAI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI