Restriction map for
pF1KB0403
(Circular) (Six-base) MAPSORT of: pF1KB0403.seq Check: 6569 from: 1 to: 4508
pF1KB0403 4508 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 353 353
Size: 4508
AccI GT'mk_AC
Cuts at: 353 1546 353
Size: 1193 3315
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 885 2333 2765 3512 3780 118
Size: 767 1448 432 747 268 846
Fragments arranged by size:
1448 846 767 747 432 268
AfeI AGC'GCT
Cuts at: 931 931
Size: 4508
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AhdI GACnn_n'nnGTC
Cuts at: 1284 1284
Size: 4508
AlwNI CAG_nnn'CTG
Cuts at: 1013 1176 1813 3380 1013
Size: 163 637 1567 2141
Fragments arranged by size:
2141 1567 637 163
ApaI G_GGCC'C
Cuts at: 1113 1113
Size: 4508
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 333 395 1528 333
Size: 62 1133 3313
Fragments arranged by size:
3313 1133 62
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 1524 1938 2186 2221 1524
Size: 414 248 35 3811
Fragments arranged by size:
3811 414 248 35
BanII G_rGCy'C
Cuts at: 1113 1522 2552 1113
Size: 409 1030 3069
Fragments arranged by size:
3069 1030 409
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbsI GAAGACnn'nnnn_
Cuts at: 353 353
Size: 4508
BbvCI CC'TCA_GC
Cuts at: 1298 1349 1298
Size: 51 4457
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 419 2028 419
Size: 1609 2899
BglI GCCn_nnn'nGGC
Cuts at: 1004 1004
Size: 4508
BglII A'GATC_T
Cuts at: 776 2023 4060 776
Size: 1247 2037 1224
Fragments arranged by size:
2037 1247 1224
BlpI GC'TnA_GC
Cuts at: 234 1620 234
Size: 1386 3122
Bme1580I G_kGCm'C
Cuts at: 1113 2133 2226 3282 1113
Size: 1020 93 1056 2339
Fragments arranged by size:
2339 1056 1020 93
BmgBI CAC'GTC
Cuts at: 672 1286 672
Size: 614 3894
BmrI ACTGGGnnnn_n'
Cuts at: 1116 1422 1471 1720 1801 2136 4280 1116
Size: 306 49 249 81 335 2144 1344
Fragments arranged by size:
2144 1344 335 306 249 81 49
BmtI G_CTAG'C
Cuts at: 917 917
Size: 4508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 4508
Bpu10I CC'TnA_GC
Cuts at: 610 1298 1349 610
Size: 688 51 3769
Fragments arranged by size:
3769 688 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 768 1487 4212 768
Size: 719 2725 1064
Fragments arranged by size:
2725 1064 719
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 281 350 953 1163 1939 2187 2889 4385 281
Size: 69 603 210 776 248 702 1496 404
Fragments arranged by size:
1496 776 702 603 404 248 210 69
BsaWI w'CCGG_w
Cuts at: 1697 2218 2896 3170 3317 3728 1697
Size: 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 748 778 1069 1099 1126 1156 490
Size: 30 228 30 291 30 27 30 3842
Fragments arranged by size:
3842 291 228 30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 574 922 1366 1369 571
Size: 3 348 444 3 3710
Fragments arranged by size:
3710 444 348 3 3
BseYI C'CCAG_C
Cuts at: 375 1019 1328 1933 2691 3268 3764 375
Size: 644 309 605 758 577 496 1119
Fragments arranged by size:
1119 758 644 605 577 496 309
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 421 685 133
Size: 288 264 3956
Fragments arranged by size:
3956 288 264
BsiEI CG_ry'CG
Cuts at: 104 315 2050 2096 3304 104
Size: 211 1735 46 1208 1308
Fragments arranged by size:
1735 1308 1208 211 46
BsiHKAI G_wGCw'C
Cuts at: 235 752 1522 2300 2490 3282 235
Size: 517 770 778 190 792 1461
Fragments arranged by size:
1461 792 778 770 517 190
Bsp1286I G_dGCh'C
Cuts at: 235 752 1113 1522 2133 2226 2300 2490 2552
Size: 517 361 409 611 93 74 190 62
Cuts at: 2552 3282 235
Size: 730 1461
Fragments arranged by size:
1461 730 611 517 409 361 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 329 415 1558 1772 2074 2455 329
Size: 86 1143 214 302 381 2382
Fragments arranged by size:
2382 1143 381 302 214 86
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1304 2420 1304
Size: 1116 3392
BsrFI r'CCGG_y
Cuts at: 629 1316 2506 2687 2896 629
Size: 687 1190 181 209 2241
Fragments arranged by size:
2241 1190 687 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 1013 1885 1013
Size: 872 3636
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 660 684 1152 1248 660
Size: 24 468 96 3920
Fragments arranged by size:
3920 468 96 24
BstYI r'GATC_y
Cuts at: 1 776 1533 2000 2023 2358 2604 3616 3702
Size: 775 757 467 23 335 246 1012 86
Cuts at: 3702 3714 4060 1
Size: 12 346 449
Fragments arranged by size:
1012 775 757 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 204 1026 1247 1361 107
Size: 97 822 221 114 3254
Fragments arranged by size:
3254 822 221 114 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 693 2458 2639 2802 693
Size: 1765 181 163 2399
Fragments arranged by size:
2399 1765 181 163
BtsI GCAGTG_nn'
Cuts at: 235 1751 235
Size: 1516 2992
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DraIII CAC_nnn'GTG
Cuts at: 857 857
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 312 674 2093 2267 2658 2685 4030 312
Size: 362 1419 174 391 27 1345 790
Fragments arranged by size:
1419 1345 790 391 362 174 27
EagI C'GGCC_G
Cuts at: 312 2093 312
Size: 1781 2727
EarI CTCTTCn'nnn_
Cuts at: 283 2531 2741 283
Size: 2248 210 2050
Fragments arranged by size:
2248 2050 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 883 2716 3026 3172 4318 883
Size: 1833 310 146 1146 1073
Fragments arranged by size:
1833 1146 1073 310 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 667 885 2333 2765 3512 3780 118
Size: 549 218 1448 432 747 268 846
Fragments arranged by size:
1448 846 747 549 432 268 218
EcoNI CCTnn'n_nnAGG
Cuts at: 1348 1348
Size: 4508
EcoO109I rG'GnC_Cy
Cuts at: 720 1341 1647 720
Size: 621 306 3581
Fragments arranged by size:
3581 621 306
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 389 630 683 933 1942 2190 3212 4191 389
Size: 241 53 250 1009 248 1022 979 706
Fragments arranged by size:
1022 1009 979 706 250 248 241 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 590 622 1126 1158 1270 1302 2350
Size: 32 242 32 504 32 112 32 1048
Cuts at: 2350 2380 2382 2412 316
Size: 30 2 30 2412
Fragments arranged by size:
2412 1048 504 242 112 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3179 3363 3179
Size: 184 4324
MscI TGG'CCA
Cuts at: 676 2269 676
Size: 1593 2915
MslI CAynn'nnrTG
Cuts at: 1246 1309 2497 2624 1246
Size: 63 1188 127 3130
Fragments arranged by size:
3130 1188 127 63
MspA1I CmG'CkG
Cuts at: 360 800 977 1001 1028 1268 1323 1363 1413
Size: 440 177 24 27 240 55 40 50
Cuts at: 1413 1446 1619 1933 2293 3306 3551 360
Size: 33 173 314 360 1013 245 1317
Fragments arranged by size:
1317 1013 440 360 314 245 240 177
173 55 50 40 33 27 24
NaeI GCC'GGC
Cuts at: 631 2689 631
Size: 2058 2450
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 107 1247 107
Size: 1140 3368
NgoMIV G'CCGG_C
Cuts at: 629 2687 629
Size: 2058 2450
NheI G'CTAG_C
Cuts at: 913 913
Size: 4508
NspI r_CATG'y
Cuts at: 252 1059 1561 2061 2968 252
Size: 807 502 500 907 1792
Fragments arranged by size:
1792 907 807 502 500
PasI CC'CwG_GG
Cuts at: 1205 1205
Size: 4508
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PfoI T'CCnGG_A
Cuts at: 275 1073 275
Size: 798 3710
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PpuMI rG'GwC_Cy
Cuts at: 720 1341 720
Size: 621 3887
PspOMI G'GGCC_C
Cuts at: 1109 1109
Size: 4508
PstI C_TGCA'G
Cuts at: 230 365 1199 1259 1412 1555 2240 230
Size: 135 834 60 153 143 685 2498
Fragments arranged by size:
2498 834 685 153 143 135 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 360 1268 1323 1413 1933 2293 360
Size: 908 55 90 520 360 2575
Fragments arranged by size:
2575 908 520 360 90 55
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SacII CC_GC'GG
Cuts at: 1029 1364 1029
Size: 335 4173
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SexAI A'CCwGG_T
Cuts at: 575 758 575
Size: 183 4325
SfcI C'TryA_G
Cuts at: 33 226 361 1195 1255 1408 1551 2236 3229
Size: 193 135 834 60 153 143 685 993
Cuts at: 3229 3420 33
Size: 191 1121
Fragments arranged by size:
1121 993 834 685 193 191 153 143
135 60
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1530 1530
Size: 4508
SmlI C'TyrA_G
Cuts at: 1663 2578 3070 3332 1663
Size: 915 492 262 2839
Fragments arranged by size:
2839 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StuI AGG'CCT
Cuts at: 898 1036 898
Size: 138 4370
StyI C'CwwG_G
Cuts at: 107 683 1247 1642 1996 107
Size: 576 564 395 354 2619
Fragments arranged by size:
2619 576 564 395 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2091 2224 2683 2892 2091
Size: 133 459 209 3707
Fragments arranged by size:
3707 459 209 133
TatI w'GTAC_w
Cuts at: 872 4023 872
Size: 3151 1357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1254 1286 4025 4057 1254
Size: 32 2739 32 1705
Fragments arranged by size:
2739 1705 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2003 2003
Size: 4508
XmaI C'CCGG_G
Cuts at: 1528 1528
Size: 4508
XmnI GAAnn'nnTTC
Cuts at: 169 169
Size: 4508
ZraI GAC'GTC
Cuts at: 351 351
Size: 4508
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI