Restriction map for
pF1KB0100
(Circular) (Six-base) MAPSORT of: pF1KB0100.seq Check: 313 from: 1 to: 6176
pF1KB0100 6176 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 484 808 484
Size: 324 5852
AccI GT'mk_AC
Cuts at: 3214 3214
Size: 6176
Acc65I G'GTAC_C
Cuts at: 3192 3192
Size: 6176
AclI AA'CG_TT
Cuts at: 6016 6016
Size: 6176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 173 3021 3118 4001 4433 4786 5448 137
Size: 36 2848 97 883 432 353 662 865
Fragments arranged by size:
2848 883 865 662 432 353 97 36
AflIII A'CryG_T
Cuts at: 1464 3528 5328 5609 1464
Size: 2064 1800 281 2031
Fragments arranged by size:
2064 2031 1800 281
AleI CACnn'nnGTG
Cuts at: 892 892
Size: 6176
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2389 2421 2389
Size: 32 6144
AlwNI CAG_nnn'CTG
Cuts at: 2217 2463 3481 4919 2217
Size: 246 1018 1438 3474
Fragments arranged by size:
3474 1438 1018 246
ApaI G_GGCC'C
Cuts at: 348 408 1794 2569 348
Size: 60 1386 775 3955
Fragments arranged by size:
3955 1386 775 60
ApaLI G'TGCA_C
Cuts at: 1588 5014 1588
Size: 3426 2750
ApoI r'AATT_y
Cuts at: 3180 5529 5785 3180
Size: 2349 256 3571
Fragments arranged by size:
3571 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6176
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
AvaI C'yCGr_G
Cuts at: 1283 2026 2093 3196 1283
Size: 743 67 1103 4263
Fragments arranged by size:
4263 1103 743 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2644 2677 2644
Size: 33 6143
BamHI G'GATC_C
Cuts at: 3201 3201
Size: 6176
BanI G'GyrC_C
Cuts at: 355 472 548 886 1274 2230 3192 3606 3854
Size: 117 76 338 388 956 962 414 248
Cuts at: 3854 3889 355
Size: 35 2642
Fragments arranged by size:
2642 962 956 414 388 338 248 117
76 35
BanII G_rGCy'C
Cuts at: 348 408 521 1070 1514 1762 1794 2569 3160
Size: 60 113 549 444 248 32 775 591
Cuts at: 3160 3190 4220 348
Size: 30 1030 2304
Fragments arranged by size:
2304 1030 775 591 549 444 248 113
60 32 30
BbeI G_GCGC'C
Cuts at: 1278 2234 3610 3858 1278
Size: 956 1376 248 3596
Fragments arranged by size:
3596 1376 956 248
BbsI GAAGACnn'nnnn_
Cuts at: 179 1070 1703 1856 1953 3071 179
Size: 891 633 153 97 1118 3284
Fragments arranged by size:
3284 1118 891 633 153 97
BbvCI CC'TCA_GC
Cuts at: 512 512
Size: 6176
BciVI GTATCCnnnnn_n'
Cuts at: 1012 2645 4074 5130 6176 1012
Size: 1633 1429 1056 1046 1012
Fragments arranged by size:
1633 1429 1056 1046 1012
BclI T'GATC_A
Cuts at: 3696 3696
Size: 6176
BglI GCCn_nnn'nGGC
Cuts at: 412 766 853 1163 1421 2212 2221 412
Size: 354 87 310 258 791 9 4367
Fragments arranged by size:
4367 791 354 310 258 87 9
BglII A'GATC_T
Cuts at: 3691 5728 3691
Size: 2037 4139
BlpI GC'TnA_GC
Cuts at: 1254 1513 1756 3050 3055 3288 1254
Size: 259 243 1294 5 233 4142
Fragments arranged by size:
4142 1294 259 243 233 5
Bme1580I G_kGCm'C
Cuts at: 348 408 889 1375 1464 1571 1592 1794 2569
Size: 60 481 486 89 107 21 202 775
Cuts at: 2569 2817 3801 3894 5018 348
Size: 248 984 93 1124 1506
Fragments arranged by size:
1506 1124 984 775 486 481 248 202
107 93 89 60 21
BmrI ACTGGGnnnn_n'
Cuts at: 123 2703 3388 3469 3804 5948 123
Size: 2580 685 81 335 2144 351
Fragments arranged by size:
2580 2144 685 351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4543 4543
Size: 6176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 435 1180 2329 2661 2741 3013 335
Size: 100 745 1149 332 80 272 3498
Fragments arranged by size:
3498 1149 745 332 272 100 80
Bpu10I CC'TnA_GC
Cuts at: 512 512
Size: 6176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 3352 4231 4945 5243 1835
Size: 1517 879 714 298 2768
Fragments arranged by size:
2768 1517 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 478 1723 5880 478
Size: 1245 4157 774
Fragments arranged by size:
4157 1245 774
BsaAI yAC'GTr
Cuts at: 738 1467 3529 738
Size: 729 2062 3385
Fragments arranged by size:
3385 2062 729
BsaBI GATnn'nnATC
Cuts at: 6 3690 5727 6
Size: 3684 2037 455
Fragments arranged by size:
3684 2037 455
BsaHI Gr'CG_yC
Cuts at: 306 1275 2231 3607 3855 4557 6053 306
Size: 969 956 1376 248 702 1496 429
Fragments arranged by size:
1496 1376 969 956 702 429 248
BsaWI w'CCGG_w
Cuts at: 226 1875 3365 3886 4564 4975 5122 5396 226
Size: 1649 1490 521 678 411 147 274 1006
Fragments arranged by size:
1649 1490 1006 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 1197 1222 1227 1252 2389 2419 2998
Size: 30 203 25 5 25 1137 30 579
Cuts at: 2998 3028 964
Size: 30 4112
Fragments arranged by size:
4112 1137 579 203 30 30 30 25
25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 594 597 600 603 606 609 612 928
Size: 65 3 3 3 3 3 3 316
Cuts at: 928 1049 1517 2140 529
Size: 121 468 623 4565
Fragments arranged by size:
4565 623 468 316 121 65 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 664 856 1084 1134 1290 1868 2274 2585 3601
Size: 192 228 50 156 578 406 311 1016
Cuts at: 3601 4359 5024 5432 664
Size: 758 665 408 1408
Fragments arranged by size:
1408 1016 758 665 578 408 406 311
228 192 156 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1188 1507 3100 943
Size: 245 319 1593 4019
Fragments arranged by size:
4019 1593 319 245
BsiEI CG_ry'CG
Cuts at: 104 1958 3718 3764 4994 104
Size: 1854 1760 46 1230 1286
Fragments arranged by size:
1854 1760 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 815 1514 1592 3190 3968 4158 5018 815
Size: 699 78 1598 778 190 860 1973
Fragments arranged by size:
1973 1598 860 778 699 190 78
BsmI GAATG_Cn'
Cuts at: 1095 1095
Size: 6176
BsmBI CGTCTCn'nnnn_
Cuts at: 832 832
Size: 6176
Bsp1286I G_dGCh'C
Cuts at: 348 408 521 815 889 1070 1375 1464 1514
Size: 60 113 294 74 181 305 89 50
Cuts at: 1514 1571 1592 1762 1794 2569 2817 3160 3190
Size: 57 21 170 32 775 248 343 30
Cuts at: 3190 3801 3894 3968 4158 4220 5018 348
Size: 611 93 74 190 62 798 1506
Fragments arranged by size:
1506 798 775 611 343 305 294 248
190 181 170 113 93 89 74 74
62 60 57 50 32 30 21
BspEI T'CCGG_A
Cuts at: 1875 1875
Size: 6176
BspHI T'CATG_A
Cuts at: 4608 4608
Size: 6176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 808 1316 1362 3077 3226 3440 3742 4123
Size: 324 508 46 1715 149 214 302 381
Cuts at: 4123 484
Size: 2537
Fragments arranged by size:
2537 1715 508 381 324 302 214 149
46
BsrBI CCG'CTC
Cuts at: 50 396 467 702 4468 4522 6006 6171 50
Size: 346 71 235 3766 54 1484 165 55
Fragments arranged by size:
3766 1484 346 235 165 71 55 54
BsrDI GCAATG_nn'
Cuts at: 2603 4088 2603
Size: 1485 4691
BsrFI r'CCGG_y
Cuts at: 1804 4174 4355 1804
Size: 2370 181 3625
Fragments arranged by size:
3625 2370 181
BssSI C'ACGA_G
Cuts at: 1031 4447 5155 1031
Size: 3416 708 2052
Fragments arranged by size:
3416 2052 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3553 3553
Size: 6176
BstBI TT'CG_AA
Cuts at: 4537 4537
Size: 6176
BstEII G'GTnAC_C
Cuts at: 2319 2319
Size: 6176
BstXI CCAn_nnnn'nTGG
Cuts at: 272 664 1651 1774 2991 272
Size: 392 987 123 1217 3457
Fragments arranged by size:
3457 1217 987 392 123
BstYI r'GATC_y
Cuts at: 1 3201 3668 3691 4026 4272 4578 4590 4676
Size: 3200 467 23 335 246 306 12 86
Cuts at: 4676 5728 1
Size: 1052 449
Fragments arranged by size:
3200 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1529 2386 2561 3145 1529
Size: 857 175 584 4560
Fragments arranged by size:
4560 857 584 175
BtgI C'CryG_G
Cuts at: 107 657 1060 1809 2179 107
Size: 550 403 749 370 4104
Fragments arranged by size:
4104 749 550 403 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 4126 4307 4470 2470
Size: 1656 181 163 4176
Fragments arranged by size:
4176 1656 181 163
BtsI GCAGTG_nn'
Cuts at: 960 1736 2276 3419 960
Size: 776 540 1143 3717
Fragments arranged by size:
3717 1143 776 540
DraI TTT'AAA
Cuts at: 3175 3175
Size: 6176
DraIII CAC_nnn'GTG
Cuts at: 390 1596 2508 2529 390
Size: 1206 912 21 4037
Fragments arranged by size:
4037 1206 912 21
DrdI GACnn_nn'nnGTC
Cuts at: 3882 5226 3882
Size: 1344 4832
EaeI y'GGCC_r
Cuts at: 1630 1764 1806 2204 2426 2550 3096 3761 3935
Size: 134 42 398 222 124 546 665 174
Cuts at: 3935 4326 4353 5698 1630
Size: 391 27 1345 2108
Fragments arranged by size:
2108 1345 665 546 398 391 222 174
134 124 42 27
EagI C'GGCC_G
Cuts at: 3761 3761
Size: 6176
EarI CTCTTCn'nnn_
Cuts at: 200 630 793 1318 4199 4409 200
Size: 430 163 525 2881 210 1967
Fragments arranged by size:
2881 1967 525 430 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4384 5126 5272 5986 4384
Size: 742 146 714 4574
Fragments arranged by size:
4574 742 714 146
EcoICRI GAG'CTC
Cuts at: 1512 3188 1512
Size: 1676 4500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 173 335 435 1180 2329 2661 2741 3013
Size: 36 162 100 745 1149 332 80 272
Cuts at: 3013 3021 3118 4001 4433 4786 5448 137
Size: 8 97 883 432 353 662 865
Fragments arranged by size:
1149 883 865 745 662 432 353 332
272 162 100 97 80 36 8
EcoO109I rG'GnC_Cy
Cuts at: 344 345 404 652 758 863 1197 1305 2064
Size: 1 59 248 106 105 334 108 759
Cuts at: 2064 2214 2565 2895 2919 3315 344
Size: 150 351 330 24 396 3205
Fragments arranged by size:
3205 759 396 351 334 330 248 150
108 106 105 59 24 1
EcoRI G'AATT_C
Cuts at: 3180 3180
Size: 6176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 6176
FspI TGC'GCA
Cuts at: 3957 3957
Size: 6176
HaeII r_GCGC'y
Cuts at: 1278 2234 3610 3858 5088 5859 1278
Size: 956 1376 248 1230 771 1595
Fragments arranged by size:
1595 1376 1230 956 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1833 1865 2998 3030 4018 4048 4050 4080 1833
Size: 32 1133 32 988 30 2 30 3929
Fragments arranged by size:
3929 1133 988 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3215 3215
Size: 6176
KasI G'GCGC_C
Cuts at: 1274 2230 3606 3854 1274
Size: 956 1376 248 3596
Fragments arranged by size:
3596 1376 956 248
KpnI G_GTAC'C
Cuts at: 3196 3196
Size: 6176
MfeI C'AATT_G
Cuts at: 369 578 369
Size: 209 5967
MluI A'CGCG_T
Cuts at: 5609 5609
Size: 6176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1634 2771 4935 5119 1208
Size: 426 1137 2164 184 2265
Fragments arranged by size:
2265 2164 1137 426 184
MscI TGG'CCA
Cuts at: 1766 2206 2428 2552 3098 3937 1766
Size: 440 222 124 546 839 4005
Fragments arranged by size:
4005 839 546 440 222 124
MslI CAynn'nnrTG
Cuts at: 892 1621 1772 1945 4165 4292 892
Size: 729 151 173 2220 127 2776
Fragments arranged by size:
2776 2220 729 173 151 127
MspA1I CmG'CkG
Cuts at: 672 880 1007 1361 1385 1755 1968 2001 2394
Size: 208 127 354 24 370 213 33 393
Cuts at: 2394 2421 2436 2838 3287 3601 3961 4745 4990
Size: 27 15 402 449 314 360 784 245
Cuts at: 4990 672
Size: 1858
Fragments arranged by size:
1858 784 449 402 393 370 360 354
314 245 213 208 127 33 27 24
15
NaeI GCC'GGC
Cuts at: 1806 4357 1806
Size: 2551 3625
NarI GG'CG_CC
Cuts at: 1275 2231 3607 3855 1275
Size: 956 1376 248 3596
Fragments arranged by size:
3596 1376 956 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6176
NgoMIV G'CCGG_C
Cuts at: 1804 4355 1804
Size: 2551 3625
NspI r_CATG'y
Cuts at: 332 449 3229 3729 5332 332
Size: 117 2780 500 1603 1176
Fragments arranged by size:
2780 1603 1176 500 117
PasI CC'CwG_GG
Cuts at: 2899 3078 2899
Size: 179 5997
PciI A'CATG_T
Cuts at: 5328 5328
Size: 6176
PflMI CCAn_nnn'nTGG
Cuts at: 2841 2841
Size: 6176
PfoI T'CCnGG_A
Cuts at: 746 1661 2769 746
Size: 915 1108 4153
Fragments arranged by size:
4153 1108 915
PmeI GTTT'AAAC
Cuts at: 3175 3175
Size: 6176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2421 2722 2754 4622 4654 2389
Size: 32 301 32 1868 32 3911
Fragments arranged by size:
3911 1868 301 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 652 1305 2064 2895 652
Size: 653 759 831 3933
Fragments arranged by size:
3933 831 759 653
PshAI GACnn'nnGTC
Cuts at: 2069 2069
Size: 6176
PspOMI G'GGCC_C
Cuts at: 344 404 1790 2565 344
Size: 60 1386 775 3955
Fragments arranged by size:
3955 1386 775 60
PstI C_TGCA'G
Cuts at: 639 1390 1539 2006 2177 2246 2420 2885 3223
Size: 751 149 467 171 69 174 465 338
Cuts at: 3223 3908 639
Size: 685 2907
Fragments arranged by size:
2907 751 685 467 465 338 174 171
149 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6176
PvuII CAG'CTG
Cuts at: 880 1755 2001 2421 2838 3601 3961 880
Size: 875 246 420 417 763 360 3095
Fragments arranged by size:
3095 875 763 420 417 360 246
RsrII CG'GwC_CG
Cuts at: 4371 4371
Size: 6176
SacI G_AGCT'C
Cuts at: 1514 3190 1514
Size: 1676 4500
SalI G'TCGA_C
Cuts at: 3213 3213
Size: 6176
SanDI GG'GwC_CC
Cuts at: 652 2895 652
Size: 2243 3933
SapI GCTCTTCn'nnn_
Cuts at: 793 1318 4199 4409 793
Size: 525 2881 210 2560
Fragments arranged by size:
2881 2560 525 210
SbfI CC_TGCA'GG
Cuts at: 2885 3223 2885
Size: 338 5838
ScaI AGT'ACT
Cuts at: 5693 5693
Size: 6176
SexAI A'CCwGG_T
Cuts at: 1592 1592
Size: 6176
SfcI C'TryA_G
Cuts at: 33 635 1386 1535 2002 2173 2242 2416 2881
Size: 602 751 149 467 171 69 174 465
Cuts at: 2881 3219 3904 4872 5063 33
Size: 338 685 968 191 1146
Fragments arranged by size:
1146 968 751 685 602 467 465 338
191 174 171 149 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 766 2212 2221 766
Size: 1446 9 4721
Fragments arranged by size:
4721 1446 9
SfoI GGC'GCC
Cuts at: 1276 2232 3608 3856 1276
Size: 956 1376 248 3596
Fragments arranged by size:
3596 1376 956 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
SmaI CCC'GGG
Cuts at: 2095 3198 2095
Size: 1103 5073
SmlI C'TyrA_G
Cuts at: 1850 3331 4246 4960 5222 1850
Size: 1481 915 714 262 2804
Fragments arranged by size:
2804 1481 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6176
SphI G_CATG'C
Cuts at: 449 3229 3729 449
Size: 2780 500 2896
Fragments arranged by size:
2896 2780 500
StuI AGG'CCT
Cuts at: 1045 1424 2910 2971 1045
Size: 379 1486 61 4250
Fragments arranged by size:
4250 1486 379 61
StyI C'CwwG_G
Cuts at: 107 566 1889 3310 3664 107
Size: 459 1323 1421 354 2619
Fragments arranged by size:
2619 1421 1323 459 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 159 448 736 3759 3892 4351 4560 159
Size: 289 288 3023 133 459 209 1775
Fragments arranged by size:
3023 1775 459 289 288 209 133
TatI w'GTAC_w
Cuts at: 5691 5691
Size: 6176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 1197 1229 1962 1994 2015 2047 5693
Size: 32 201 32 733 32 21 32 3646
Cuts at: 5693 5725 964
Size: 32 1415
Fragments arranged by size:
3646 1415 733 201 32 32 32 32
32 21
Tth111I GACn'n_nGTC
Cuts at: 3973 3973
Size: 6176
XbaI T'CTAG_A
Cuts at: 3207 3207
Size: 6176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 269 1947 3671 269
Size: 1678 1724 2774
Fragments arranged by size:
2774 1724 1678
XmaI C'CCGG_G
Cuts at: 2093 3196 2093
Size: 1103 5073
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI AvrII
BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI PsrI SacII SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI