Restriction map for
pF1KA1997
(Circular) (Six-base) MAPSORT of: pF1KA1997.seq Check: 9867 from: 1 to: 16034
pF1KA1997 16034 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1296 1296
Size: 16034
AccI GT'mk_AC
Cuts at: 4438 5059 7142 12726 13072 4438
Size: 621 2083 5584 346 7400
Fragments arranged by size:
7400 5584 2083 621 346
Acc65I G'GTAC_C
Cuts at: 13050 13050
Size: 16034
AclI AA'CG_TT
Cuts at: 11241 15874 11241
Size: 4633 11401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 493 3098 3223 4489 4772 4991 7489 8188
Size: 116 2605 125 1266 283 219 2498 699
Cuts at: 8188 8285 8995 9487 9497 9548 9608 11605 11685
Size: 97 710 492 10 51 60 1997 80
Cuts at: 11685 11911 11942 11959 13859 14291 15038 15306 377
Size: 226 31 17 1900 432 747 268 1105
Fragments arranged by size:
2605 2498 1997 1900 1266 1105 747 710
699 492 432 283 268 226 219 125
116 97 80 60 51 31 17 10
AfeI AGC'GCT
Cuts at: 1030 2257 1030
Size: 1227 14807
AflII C'TTAA_G
Cuts at: 6835 8359 6835
Size: 1524 14510
AflIII A'CryG_T
Cuts at: 5023 8193 8736 10649 10904 13386 14490 15467 5023
Size: 3170 543 1913 255 2482 1104 977 5590
Fragments arranged by size:
5590 3170 2482 1913 1104 977 543 255
AgeI A'CCGG_T
Cuts at: 14422 14422
Size: 16034
AhdI GACnn_n'nnGTC
Cuts at: 7809 7809
Size: 16034
AleI CACnn'nnGTG
Cuts at: 204 204
Size: 16034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 7018 12928 268
Size: 6750 5910 3374
Fragments arranged by size:
6750 5910 3374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 10721 10753 878
Size: 32 9811 32 6159
Fragments arranged by size:
9811 6159 32 32
AlwNI CAG_nnn'CTG
Cuts at: 201 7098 8908 9571 13339 14906 201
Size: 6897 1810 663 3768 1567 1329
Fragments arranged by size:
6897 3768 1810 1567 1329 663
ApaLI G'TGCA_C
Cuts at: 7064 10511 14804 7064
Size: 3447 4293 8294
Fragments arranged by size:
8294 4293 3447
ApoI r'AATT_y
Cuts at: 586 1334 1502 1933 2061 2418 2760 3157 3265
Size: 748 168 431 128 357 342 397 108
Cuts at: 3265 3494 3589 4128 4302 4357 4744 4952 5050
Size: 229 95 539 174 55 387 208 98
Cuts at: 5050 5332 5451 5465 6306 6667 6727 7278 7502
Size: 282 119 14 841 361 60 551 224
Cuts at: 7502 7555 7977 8420 8463 8640 9113 9422 9547
Size: 53 422 443 43 177 473 309 125
Cuts at: 9547 9817 10201 10553 11622 11776 11854 12065 12196
Size: 270 384 352 1069 154 78 211 131
Cuts at: 12196 12264 12703 13038 15387 15643 586
Size: 68 439 335 2349 256 977
Fragments arranged by size:
2349 1069 977 841 748 551 539 473
443 439 431 422 397 387 384 361
357 352 342 335 309 282 270 256
229 224 211 208 177 174 168 154
131 128 125 119 108 98 95 78
68 60 55 53 43 14
AseI AT'TA_AT
Cuts at: 20 2837 3940 6677 8677 9635 10643 20
Size: 2817 1103 2737 2000 958 1008 5411
Fragments arranged by size:
5411 2817 2737 2000 1103 1008 958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 16034
AvaI C'yCGr_G
Cuts at: 1184 13054 1184
Size: 11870 4164
AvrII C'CTAG_G
Cuts at: 3766 3766
Size: 16034
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2936 2969 3812 3845 7519 7552 2936
Size: 33 843 33 3674 33 11418
Fragments arranged by size:
11418 3674 843 33 33 33
BamHI G'GATC_C
Cuts at: 4854 7648 13059 4854
Size: 2794 5411 7829
Fragments arranged by size:
7829 5411 2794
BanI G'GyrC_C
Cuts at: 1493 11833 13050 13464 13712 13747 1493
Size: 10340 1217 414 248 35 3780
Fragments arranged by size:
10340 3780 1217 414 248 35
BanII G_rGCy'C
Cuts at: 1244 7792 7842 8345 9720 10698 11691 13048 14078
Size: 6548 50 503 1375 978 993 1357 1030
Cuts at: 14078 1244
Size: 3200
Fragments arranged by size:
6548 3200 1375 1357 1030 993 978 503
50
BbeI G_GCGC'C
Cuts at: 13468 13716 13468
Size: 248 15786
BbsI GAAGACnn'nnnn_
Cuts at: 2860 3086 3229 3554 3959 4777 4979 5235 5514
Size: 226 143 325 405 818 202 256 279
Cuts at: 5514 5722 6854 7344 7353 8291 8391 9248 9485
Size: 208 1132 490 9 938 100 857 237
Cuts at: 9485 2860
Size: 9409
Fragments arranged by size:
9409 1132 938 857 818 490 405 325
279 256 237 226 208 202 143 100
9
BbvCI CC'TCA_GC
Cuts at: 5681 5681
Size: 16034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3469 3503 10816 10850 3469
Size: 34 7313 34 8653
Fragments arranged by size:
8653 7313 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 622 6783 10869 13932 14693 16034 622
Size: 6161 4086 3063 761 1341 622
Fragments arranged by size:
6161 4086 3063 1341 761 622
BclI T'GATC_A
Cuts at: 828 1989 4323 5016 8282 8921 10563 10806 10941
Size: 1161 2334 693 3266 639 1642 243 135
Cuts at: 10941 12134 13554 828
Size: 1193 1420 3308
Fragments arranged by size:
3308 3266 2334 1642 1420 1193 1161 693
639 243 135
BfrBI ATG'CAT
Cuts at: 1516 2334 2338 8121 10845 12877 1516
Size: 818 4 5783 2724 2032 4673
Fragments arranged by size:
5783 4673 2724 2032 818 4
BglII A'GATC_T
Cuts at: 1671 1756 2640 3242 6468 10134 11790 13549 15586
Size: 85 884 602 3226 3666 1656 1759 2037
Cuts at: 15586 1671
Size: 2119
Fragments arranged by size:
3666 3226 2119 2037 1759 1656 884 602
85
BlpI GC'TnA_GC
Cuts at: 2292 4377 9344 13146 2292
Size: 2085 4967 3802 5180
Fragments arranged by size:
5180 4967 3802 2085
Bme1580I G_kGCm'C
Cuts at: 3492 3870 6756 7068 9940 10515 11421 12552 13659
Size: 378 2886 312 2872 575 906 1131 1107
Cuts at: 13659 13752 14808 3492
Size: 93 1056 4718
Fragments arranged by size:
4718 2886 2872 1131 1107 1056 906 575
378 312 93
BmgBI CAC'GTC
Cuts at: 3235 3235
Size: 16034
BmrI ACTGGGnnnn_n'
Cuts at: 2982 4679 4986 5195 6338 7351 11003 12576 13246
Size: 1697 307 209 1143 1013 3652 1573 670
Cuts at: 13246 13327 13662 15806 2982
Size: 81 335 2144 3210
Fragments arranged by size:
3652 3210 2144 1697 1573 1143 1013 670
335 307 209 81
BmtI G_CTAG'C
Cuts at: 10408 10408
Size: 16034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11452 12924 14401 11452
Size: 1472 1477 13085
Fragments arranged by size:
13085 1477 1472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2398 3710 4903 6752 7352 8069 8882 11591 2398
Size: 1312 1193 1849 600 717 813 2709 6841
Fragments arranged by size:
6841 2709 1849 1312 1193 813 717 600
Bpu10I CC'TnA_GC
Cuts at: 2037 5681 7009 11654 2037
Size: 3644 1328 4645 6417
Fragments arranged by size:
6417 4645 3644 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4667 5120 6227 7912 8263 9601 9832 10130 10433
Size: 453 1107 1685 351 1338 231 298 303
Cuts at: 10433 13210 14089 14581 14879 4667
Size: 2777 879 492 298 5822
Fragments arranged by size:
5822 2777 1685 1338 1107 879 492 453
351 303 298 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 15738 15738
Size: 16034
BsaAI yAC'GTr
Cuts at: 3901 10939 13387 3901
Size: 7038 2448 6548
Fragments arranged by size:
7038 6548 2448
BsaBI GATnn'nnATC
Cuts at: 6 374 833 3014 3419 6323 9181 9456 10580
Size: 368 459 2181 405 2904 2858 275 1124
Cuts at: 10580 12794 13548 15585 6
Size: 2214 754 2037 455
Fragments arranged by size:
2904 2858 2214 2181 2037 1124 754 459
455 405 368 275
BsaHI Gr'CG_yC
Cuts at: 13465 13713 14415 15911 13465
Size: 248 702 1496 13588
Fragments arranged by size:
13588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 7031 10248 11154 12894 13223 13744 14422 14696 14843
Size: 3217 906 1740 329 521 678 274 147
Cuts at: 14843 15254 7031
Size: 411 7811
Fragments arranged by size:
7811 3217 1740 906 678 521 411 329
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2371 2401 4541 4571 8002 8032 8524 8554 2371
Size: 30 2140 30 3431 30 492 30 9851
Fragments arranged by size:
9851 3431 2140 492 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 6755 9514 9843 12284 247
Size: 6508 2759 329 2441 3997
Fragments arranged by size:
6508 3997 2759 2441 329
BseYI C'CCAG_C
Cuts at: 535 10976 11800 12826 13459 14217 14794 15290 535
Size: 10441 824 1026 633 758 577 496 1279
Fragments arranged by size:
10441 1279 1026 824 758 633 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1862 4709 9350 286
Size: 1576 2847 4641 6970
Fragments arranged by size:
6970 4641 2847 1576
BsiEI CG_ry'CG
Cuts at: 104 4179 12306 13576 13622 14830 104
Size: 4075 8127 1270 46 1208 1308
Fragments arranged by size:
8127 4075 1308 1270 1208 46
BsiHKAI G_wGCw'C
Cuts at: 6222 7068 7842 9720 10515 10698 11691 12845 13048
Size: 846 774 1878 795 183 993 1154 203
Cuts at: 13048 13826 14016 14808 6222
Size: 778 190 792 7448
Fragments arranged by size:
7448 1878 1154 993 846 795 792 778
774 203 190 183
BsmI GAATG_Cn'
Cuts at: 1366 2531 4381 5784 7234 7598 9345 9672 1366
Size: 1165 1850 1403 1450 364 1747 327 7728
Fragments arranged by size:
7728 1850 1747 1450 1403 1165 364 327
BsmBI CGTCTCn'nnnn_
Cuts at: 3757 10122 3757
Size: 6365 9669
Bsp1286I G_dGCh'C
Cuts at: 1244 3492 3870 6222 6756 7068 7792 7842 8345
Size: 2248 378 2352 534 312 724 50 503
Cuts at: 8345 9720 9940 10515 10698 11421 11691 12552 12845
Size: 1375 220 575 183 723 270 861 293
Cuts at: 12845 13048 13659 13752 13826 14016 14078 14808 1244
Size: 203 611 93 74 190 62 730 2470
Fragments arranged by size:
2470 2352 2248 1375 861 730 724 723
611 575 534 503 378 312 293 270
220 203 190 183 93 74 62 50
BspEI T'CCGG_A
Cuts at: 12894 15254 12894
Size: 2360 13674
BspHI T'CATG_A
Cuts at: 1128 6396 6660 8474 15210 1128
Size: 5268 264 1814 6736 1952
Fragments arranged by size:
6736 5268 1952 1814 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1161 1296 4130 5931 13084 13298 13600 13981 1161
Size: 135 2834 1801 7153 214 302 381 3214
Fragments arranged by size:
7153 3214 2834 1801 381 302 214 135
BsrBI CCG'CTC
Cuts at: 50 8128 14326 14380 15864 16029 50
Size: 8078 6198 54 1484 165 55
Fragments arranged by size:
8078 6198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 4186 4538 11430 13946 1922
Size: 2264 352 6892 2516 4010
Fragments arranged by size:
6892 4010 2516 2264 352
BsrFI r'CCGG_y
Cuts at: 14032 14213 14422 14032
Size: 181 209 15644
Fragments arranged by size:
15644 209 181
BsrGI T'GTAC_A
Cuts at: 2366 2742 8259 11844 11859 2366
Size: 376 5517 3585 15 6541
Fragments arranged by size:
6541 5517 3585 376 15
BssSI C'ACGA_G
Cuts at: 605 6755 14305 14663 605
Size: 6150 7550 358 1976
Fragments arranged by size:
7550 6150 1976 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 8433 8704 8908 10510 13411 1924
Size: 6509 271 204 1602 2901 4547
Fragments arranged by size:
6509 4547 2901 1602 271 204
BstBI TT'CG_AA
Cuts at: 14395 14395
Size: 16034
BstEII G'GTnAC_C
Cuts at: 12948 12948
Size: 16034
BstXI CCAn_nnnn'nTGG
Cuts at: 3734 5148 7227 3734
Size: 1414 2079 12541
Fragments arranged by size:
12541 2079 1414
BstYI r'GATC_y
Cuts at: 1 930 1671 1756 2640 3242 4854 6468 7519
Size: 929 741 85 884 602 1612 1614 1051
Cuts at: 7519 7648 9951 10134 11790 12175 12313 12513 13059
Size: 129 2303 183 1656 385 138 200 546
Cuts at: 13059 13526 13549 13884 14130 15142 15228 15240 15586
Size: 467 23 335 246 1012 86 12 346
Cuts at: 15586 1
Size: 449
Fragments arranged by size:
2303 1656 1614 1612 1051 1012 929 884
741 602 546 467 449 385 346 335
246 200 183 138 129 86 85 23
12
BstZ17I GTA'TAC
Cuts at: 5060 7143 5060
Size: 2083 13951
Bsu36I CC'TnA_GG
Cuts at: 259 11753 259
Size: 11494 4540
BtgI C'CryG_G
Cuts at: 107 660 1045 4179 6937 10982 11174 11369 107
Size: 553 385 3134 2758 4045 192 195 4772
Fragments arranged by size:
4772 4045 3134 2758 553 385 195 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 641 13984 14165 14328 641
Size: 13343 181 163 2347
Fragments arranged by size:
13343 2347 181 163
BtsI GCAGTG_nn'
Cuts at: 3935 8082 10515 11221 11534 12683 13277 3935
Size: 4147 2433 706 313 1149 594 6692
Fragments arranged by size:
6692 4147 2433 1149 706 594 313
ClaI AT'CG_AT
Cuts at: 5264 5264
Size: 16034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1433 1468 7026 7061 12659 12694 12970 13005 1433
Size: 35 5558 35 5598 35 276 35 4462
Fragments arranged by size:
5598 5558 4462 276 35 35 35 35
DraI TTT'AAA
Cuts at: 702 2766 2831 3270 3857 4133 4566 5613 9312
Size: 2064 65 439 587 276 433 1047 3699
Cuts at: 9312 11615 13033 702
Size: 2303 1418 3703
Fragments arranged by size:
3703 3699 2303 2064 1418 1047 587 439
433 276 65
DraIII CAC_nnn'GTG
Cuts at: 3874 3874
Size: 16034
DrdI GACnn_nn'nnGTC
Cuts at: 4267 13740 14598 4267
Size: 9473 858 5703
Fragments arranged by size:
9473 5703 858
EaeI y'GGCC_r
Cuts at: 3318 3539 4176 13619 13793 14184 14211 15556 3318
Size: 221 637 9443 174 391 27 1345 3796
Fragments arranged by size:
9443 3796 1345 637 391 221 174 27
EagI C'GGCC_G
Cuts at: 4176 13619 4176
Size: 9443 6591
EarI CTCTTCn'nnn_
Cuts at: 145 1097 1157 2512 3431 3506 5945 6455 6890
Size: 952 60 1355 919 75 2439 510 435
Cuts at: 6890 9710 10430 11507 11704 12599 12659 14057 14267
Size: 2820 720 1077 197 895 60 1398 210
Cuts at: 14267 145
Size: 1912
Fragments arranged by size:
2820 2439 1912 1398 1355 1077 952 919
895 720 510 435 210 197 75 60
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4747 10195 14242 14552 14698 15844 4747
Size: 5448 4047 310 146 1146 4937
Fragments arranged by size:
5448 4937 4047 1146 310 146
EcoICRI GAG'CTC
Cuts at: 7840 9718 10696 11689 13046 7840
Size: 1878 978 993 1357 10828
Fragments arranged by size:
10828 1878 1357 993 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 493 2398 3098 3223 3710 4489 4772 4903
Size: 116 1905 700 125 487 779 283 131
Cuts at: 4903 4991 6752 7352 7489 8069 8188 8285 8882
Size: 88 1761 600 137 580 119 97 597
Cuts at: 8882 8995 9487 9497 9548 9608 11591 11605 11685
Size: 113 492 10 51 60 1983 14 80
Cuts at: 11685 11911 11942 11959 13859 14291 15038 15306 377
Size: 226 31 17 1900 432 747 268 1105
Fragments arranged by size:
1983 1905 1900 1761 1105 779 747 700
600 597 580 492 487 432 283 268
226 137 131 125 119 116 113 97
88 80 60 51 31 17 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1102 3583 7243 1102
Size: 2481 3660 9893
Fragments arranged by size:
9893 3660 2481
EcoO109I rG'GnC_Cy
Cuts at: 2474 5312 13173 2474
Size: 2838 7861 5335
Fragments arranged by size:
7861 5335 2838
EcoRI G'AATT_C
Cuts at: 1502 2061 6306 6667 8420 9422 10201 12264 13038
Size: 559 4245 361 1753 1002 779 2063 774
Cuts at: 13038 1502
Size: 4498
Fragments arranged by size:
4498 4245 2063 1753 1002 779 774 559
361
EcoRV GAT'ATC
Cuts at: 3477 4251 6154 7188 3477
Size: 774 1903 1034 12323
Fragments arranged by size:
12323 1903 1034 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1451 5139 5961 9322 10441 10546 10632 14053 1451
Size: 3688 822 3361 1119 105 86 3421 3432
Fragments arranged by size:
3688 3432 3421 3361 1119 822 105 86
FspI TGC'GCA
Cuts at: 13815 13815
Size: 16034
HaeII r_GCGC'y
Cuts at: 1032 2259 7707 13468 13716 14738 15717 1032
Size: 1227 5448 5761 248 1022 979 1349
Fragments arranged by size:
5761 5448 1349 1227 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 1705 1720 1737 1741 1752 1773 1992
Size: 32 937 15 17 4 11 21 219
Cuts at: 1992 2024 2853 2885 3162 3194 3234 3266 4016
Size: 32 829 32 277 32 40 32 750
Cuts at: 4016 4048 4066 4098 4219 4251 4446 4478 4498
Size: 32 18 32 121 32 195 32 20
Cuts at: 4498 4530 5257 5289 6238 6270 6285 6317 7774
Size: 32 727 32 949 32 15 32 1457
Cuts at: 7774 7795 7806 7827 8002 8034 8524 8556 10119
Size: 21 11 21 175 32 490 32 1563
Cuts at: 10119 10151 11077 11109 12126 12158 13876 13906 13908
Size: 32 926 32 1017 32 1718 30 2
Cuts at: 13908 13938 736
Size: 30 2832
Fragments arranged by size:
2832 1718 1563 1457 1017 949 937 926
829 750 727 490 277 219 195 175
121 40 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 21 21
21 20 18 17 15 15 11 11
4 2
HincII GTy'rAC
Cuts at: 771 1557 3669 4695 6378 10220 10278 12727 13073
Size: 786 2112 1026 1683 3842 58 2449 346
Cuts at: 13073 771
Size: 3732
Fragments arranged by size:
3842 3732 2449 2112 1683 1026 786 346
58
HindIII A'AGCT_T
Cuts at: 3893 4474 8369 3893
Size: 581 3895 11558
Fragments arranged by size:
11558 3895 581
HpaI GTT'AAC
Cuts at: 6378 10278 6378
Size: 3900 12134
KasI G'GCGC_C
Cuts at: 13464 13712 13464
Size: 248 15786
KpnI G_GTAC'C
Cuts at: 13054 13054
Size: 16034
MfeI C'AATT_G
Cuts at: 719 3824 719
Size: 3105 12929
MluI A'CGCG_T
Cuts at: 15467 15467
Size: 16034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 320 1423 1562 3903 3993 8071 9063 10704
Size: 21 1103 139 2341 90 4078 992 1641
Cuts at: 10704 10713 10878 11740 14705 14889 299
Size: 9 165 862 2965 184 1444
Fragments arranged by size:
4078 2965 2341 1641 1444 1103 992 862
184 165 139 90 21 9
MscI TGG'CCA
Cuts at: 3320 3541 13795 3320
Size: 221 10254 5559
Fragments arranged by size:
10254 5559 221
MslI CAynn'nnrTG
Cuts at: 204 1661 2933 5146 5266 6644 7061 8120 14023
Size: 1457 1272 2213 120 1378 417 1059 5903
Cuts at: 14023 14150 204
Size: 127 2088
Fragments arranged by size:
5903 2213 2088 1457 1378 1272 1059 417
127 120
MspA1I CmG'CkG
Cuts at: 2662 5179 7330 9364 9805 12187 13145 13459 13819
Size: 2517 2151 2034 441 2382 958 314 360
Cuts at: 13819 14832 15077 2662
Size: 1013 245 3619
Fragments arranged by size:
3619 2517 2382 2151 2034 1013 958 441
360 314 245
NaeI GCC'GGC
Cuts at: 14215 14215
Size: 16034
NarI GG'CG_CC
Cuts at: 13465 13713 13465
Size: 248 15786
NcoI C'CATG_G
Cuts at: 107 1045 6937 11174 11369 107
Size: 938 5892 4237 195 4772
Fragments arranged by size:
5892 4772 4237 938 195
NdeI CA'TA_TG
Cuts at: 1174 6828 7692 8700 10766 11595 1174
Size: 5654 864 1008 2066 829 5613
Fragments arranged by size:
5654 5613 2066 1008 864 829
NgoMIV G'CCGG_C
Cuts at: 14213 14213
Size: 16034
NheI G'CTAG_C
Cuts at: 10404 10404
Size: 16034
NsiI A_TGCA'T
Cuts at: 1518 2336 2340 8123 10847 12879 1518
Size: 818 4 5783 2724 2032 4673
Fragments arranged by size:
5783 4673 2724 2032 818 4
NspI r_CATG'y
Cuts at: 2334 2338 5027 6912 8121 8197 8740 9132 10653
Size: 4 2689 1885 1209 76 543 392 1521
Cuts at: 10653 10845 10908 11192 11729 12881 13087 13587 14494
Size: 192 63 284 537 1152 206 500 907
Cuts at: 14494 2334
Size: 3874
Fragments arranged by size:
3874 2689 1885 1521 1209 1152 907 543
537 500 392 284 206 192 76 63
4
PacI TTA_AT'TAA
Cuts at: 9635 9635
Size: 16034
PciI A'CATG_T
Cuts at: 5023 8193 8736 10649 10904 14490 5023
Size: 3170 543 1913 255 3586 6567
Fragments arranged by size:
6567 3586 3170 1913 543 255
PflMI CCAn_nnn'nTGG
Cuts at: 1531 2994 6024 9414 1531
Size: 1463 3030 3390 8151
Fragments arranged by size:
8151 3390 3030 1463
PfoI T'CCnGG_A
Cuts at: 4880 5886 8313 4880
Size: 1006 2427 12601
Fragments arranged by size:
12601 2427 1006
PmeI GTTT'AAAC
Cuts at: 13033 13033
Size: 16034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 15173 15205 15173
Size: 32 16002
PsiI TTA'TAA
Cuts at: 737 1979 4061 11063 737
Size: 1242 2082 7002 5708
Fragments arranged by size:
7002 5708 2082 1242
PspXI vC'TCGA_Gb
Cuts at: 1184 1184
Size: 16034
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7439 7471 10094 10126 7439
Size: 32 2623 32 13347
Fragments arranged by size:
13347 2623 32 32
PstI C_TGCA'G
Cuts at: 4127 7094 9363 9624 11541 12034 13081 13766 4127
Size: 2967 2269 261 1917 493 1047 685 6395
Fragments arranged by size:
6395 2967 2269 1917 1047 685 493 261
PvuI CG_AT'CG
Cuts at: 104 12306 104
Size: 12202 3832
PvuII CAG'CTG
Cuts at: 2662 5179 7330 9364 9805 12187 13459 13819 2662
Size: 2517 2151 2034 441 2382 1272 360 4877
Fragments arranged by size:
4877 2517 2382 2151 2034 1272 441 360
RsrII CG'GwC_CG
Cuts at: 14229 14229
Size: 16034
SacI G_AGCT'C
Cuts at: 7842 9720 10698 11691 13048 7842
Size: 1878 978 993 1357 10828
Fragments arranged by size:
10828 1878 1357 993 978
SalI G'TCGA_C
Cuts at: 12725 13071 12725
Size: 346 15688
SapI GCTCTTCn'nnn_
Cuts at: 145 3431 12599 12659 14057 14267 145
Size: 3286 9168 60 1398 210 1912
Fragments arranged by size:
9168 3286 1912 1398 210 60
SbfI CC_TGCA'GG
Cuts at: 13081 13081
Size: 16034
ScaI AGT'ACT
Cuts at: 7125 7224 9357 15551 7125
Size: 99 2133 6194 7608
Fragments arranged by size:
7608 6194 2133 99
SexAI A'CCwGG_T
Cuts at: 11360 11360
Size: 16034
SfcI C'TryA_G
Cuts at: 33 2300 4123 7049 7090 7394 9359 9620 9968
Size: 2267 1823 2926 41 304 1965 261 348
Cuts at: 9968 11537 11572 12030 13077 13762 14755 14946 33
Size: 1569 35 458 1047 685 993 191 1121
Fragments arranged by size:
2926 2267 1965 1823 1569 1121 1047 993
685 458 348 304 261 191 41 35
SfoI GGC'GCC
Cuts at: 13466 13714 13466
Size: 248 15786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 16034
SmaI CCC'GGG
Cuts at: 13056 13056
Size: 16034
SmlI C'TyrA_G
Cuts at: 1184 4682 5135 6242 6835 7927 8242 8359 9580
Size: 3498 453 1107 593 1092 315 117 1221
Cuts at: 9580 9811 10145 10448 13189 14104 14596 14858 1184
Size: 231 334 303 2741 915 492 262 2360
Fragments arranged by size:
3498 2741 2360 1221 1107 1092 915 593
492 453 334 315 303 262 231 117
SpeI A'CTAG_T
Cuts at: 67 3775 67
Size: 3708 12326
SphI G_CATG'C
Cuts at: 2338 9132 10845 13087 13587 2338
Size: 6794 1713 2242 500 4785
Fragments arranged by size:
6794 4785 2242 1713 500
SspI AAT'ATT
Cuts at: 1063 2232 2503 2835 4427 4926 5288 7305 9270
Size: 1169 271 332 1592 499 362 2017 1965
Cuts at: 9270 9396 9920 10458 12957 1063
Size: 126 524 538 2499 4140
Fragments arranged by size:
4140 2499 2017 1965 1592 1169 538 524
499 362 332 271 126
StuI AGG'CCT
Cuts at: 9878 9878
Size: 16034
StyI C'CwwG_G
Cuts at: 107 1045 3756 3766 6937 11174 11369 13168 13522
Size: 938 2711 10 3171 4237 195 1799 354
Cuts at: 13522 107
Size: 2619
Fragments arranged by size:
4237 3171 2711 2619 1799 938 354 195
10
SwaI ATTT'AAAT
Cuts at: 3857 4133 3857
Size: 276 15758
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 978 2545 4143 9189 9372 13617 13750 14209 14418
Size: 1567 1598 5046 183 4245 133 459 209
Cuts at: 14418 978
Size: 2594
Fragments arranged by size:
5046 4245 2594 1598 1567 459 209 183
133
TatI w'GTAC_w
Cuts at: 2366 2742 5355 7123 7175 7222 8259 9048 9355
Size: 376 2613 1768 52 47 1037 789 307
Cuts at: 9355 9841 10613 11139 11844 11859 12366 12438 15549
Size: 486 772 526 705 15 507 72 3111
Cuts at: 15549 2366
Size: 2851
Fragments arranged by size:
3111 2851 2613 1768 1037 789 772 705
526 507 486 376 307 72 52 47
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 596 628 2300 2332 5466 5498 6252 6284 12984
Size: 32 1672 32 3134 32 754 32 6700
Cuts at: 12984 13016 15551 15583 596
Size: 32 2535 32 1047
Fragments arranged by size:
6700 3134 2535 1672 1047 754 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8789 9798 13831 8789
Size: 1009 4033 10992
Fragments arranged by size:
10992 4033 1009
XbaI T'CTAG_A
Cuts at: 3780 6430 6921 7296 10428 13065 3780
Size: 2650 491 375 3132 2637 6749
Fragments arranged by size:
6749 3132 2650 2637 491 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3292 7615 13529 3292
Size: 4323 5914 5797
Fragments arranged by size:
5914 5797 4323
XhoI C'TCGA_G
Cuts at: 1184 1184
Size: 16034
XmaI C'CCGG_G
Cuts at: 13054 13054
Size: 16034
XmnI GAAnn'nnTTC
Cuts at: 139 166 2521 2801 3515 5591 6356 7304 7451
Size: 27 2355 280 714 2076 765 948 147
Cuts at: 7451 8173 9214 9270 9302 9422 9548 10109 10424
Size: 722 1041 56 32 120 126 561 315
Cuts at: 10424 10856 139
Size: 432 5317
Fragments arranged by size:
5317 2355 2076 1041 948 765 722 714
561 432 315 280 147 126 120 56
32 27
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII ApaI AscI BglI BsiWI BssHII FseI FspAI
NotI NruI PasI PmlI PpuMI PshAI PspOMI SacII
SanDI SfiI SgrAI SnaBI SrfI ZraI