Restriction map for
pF1KA1930
(Circular) (Six-base) MAPSORT of: pF1KA1930.seq Check: 747 from: 1 to: 6770
pF1KA1930 6770 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 837 837
Size: 6770
AccI GT'mk_AC
Cuts at: 2347 3808 2347
Size: 1461 5309
Acc65I G'GTAC_C
Cuts at: 1024 3786 1024
Size: 2762 4008
AclI AA'CG_TT
Cuts at: 6610 6610
Size: 6770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 370 940 1235 1481 1706 2640 3364 4595
Size: 160 570 295 246 225 934 724 1231
Cuts at: 4595 5027 5774 6042 210
Size: 432 747 268 938
Fragments arranged by size:
1231 938 934 747 724 570 432 295
268 246 225 160
AfeI AGC'GCT
Cuts at: 808 1126 808
Size: 318 6452
AflIII A'CryG_T
Cuts at: 4122 5226 6203 4122
Size: 1104 977 4689
Fragments arranged by size:
4689 1104 977
AgeI A'CCGG_T
Cuts at: 5158 5158
Size: 6770
AleI CACnn'nnGTG
Cuts at: 1460 1460
Size: 6770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 6770
AlwNI CAG_nnn'CTG
Cuts at: 1285 1648 1669 1714 2209 2547 3177 3202 3631
Size: 363 21 45 495 338 630 25 429
Cuts at: 3631 4075 5642 1285
Size: 444 1567 2413
Fragments arranged by size:
2413 1567 630 495 444 429 363 338
45 25 21
ApaI G_GGCC'C
Cuts at: 209 242 1385 2496 2861 2886 3399 3478 3498
Size: 33 1143 1111 365 25 513 79 20
Cuts at: 3498 209
Size: 3481
Fragments arranged by size:
3481 1143 1111 513 365 79 33 25
20
ApaLI G'TGCA_C
Cuts at: 379 3027 5540 379
Size: 2648 2513 1609
Fragments arranged by size:
2648 2513 1609
ApoI r'AATT_y
Cuts at: 432 892 3774 6123 6379 432
Size: 460 2882 2349 256 823
Fragments arranged by size:
2882 2349 823 460 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6770
AvaI C'yCGr_G
Cuts at: 152 266 319 609 626 650 1327 2909 3364
Size: 114 53 290 17 24 677 1582 455
Cuts at: 3364 3542 3583 3790 152
Size: 178 41 207 3132
Fragments arranged by size:
3132 1582 677 455 290 207 178 114
53 41 24 17
AvrII C'CTAG_G
Cuts at: 1306 3564 1306
Size: 2258 4512
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1008 1041 1008
Size: 33 6737
BamHI G'GATC_C
Cuts at: 3795 3795
Size: 6770
BanI G'GyrC_C
Cuts at: 309 571 1024 2810 3097 3360 3786 4200 4448
Size: 262 453 1786 287 263 426 414 248
Cuts at: 4448 4483 309
Size: 35 2596
Fragments arranged by size:
2596 1786 453 426 414 287 263 262
248 35
BanII G_rGCy'C
Cuts at: 209 242 1060 1150 1334 1385 2496 2861 2886
Size: 33 818 90 184 51 1111 365 25
Cuts at: 2886 3399 3478 3498 3599 3784 4814 209
Size: 513 79 20 101 185 1030 2165
Fragments arranged by size:
2165 1111 1030 818 513 365 185 184
101 90 79 51 33 25 20
BbeI G_GCGC'C
Cuts at: 3364 4204 4452 3364
Size: 840 248 5682
Fragments arranged by size:
5682 840 248
BbsI GAAGACnn'nnnn_
Cuts at: 216 2678 2690 3213 216
Size: 2462 12 523 3773
Fragments arranged by size:
3773 2462 523 12
BbvCI CC'TCA_GC
Cuts at: 1083 1490 2735 2770 3131 3290 3400 1083
Size: 407 1245 35 361 159 110 4453
Fragments arranged by size:
4453 1245 407 361 159 110 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3744 3778 3744
Size: 34 6736
BciVI GTATCCnnnnn_n'
Cuts at: 4668 5429 6770 4668
Size: 761 1341 4668
Fragments arranged by size:
4668 1341 761
BclI T'GATC_A
Cuts at: 3327 4290 3327
Size: 963 5807
BglI GCCn_nnn'nGGC
Cuts at: 324 2500 3122 3186 3369 324
Size: 2176 622 64 183 3725
Fragments arranged by size:
3725 2176 622 183 64
BglII A'GATC_T
Cuts at: 782 4285 6322 782
Size: 3503 2037 1230
Fragments arranged by size:
3503 2037 1230
BlpI GC'TnA_GC
Cuts at: 145 1036 3882 145
Size: 891 2846 3033
Fragments arranged by size:
3033 2846 891
Bme1580I G_kGCm'C
Cuts at: 209 242 383 1385 2496 2763 2851 2861 2886
Size: 33 141 1002 1111 267 88 10 25
Cuts at: 2886 3031 3102 3399 3478 3498 4395 4488 5544
Size: 145 71 297 79 20 897 93 1056
Cuts at: 5544 209
Size: 1435
Fragments arranged by size:
1435 1111 1056 1002 897 297 267 145
141 93 88 79 71 33 25 20
10
BmgBI CAC'GTC
Cuts at: 3534 3534
Size: 6770
BmrI ACTGGGnnnn_n'
Cuts at: 611 1405 1574 1882 2036 2227 2431 2497 2750
Size: 794 169 308 154 191 204 66 253
Cuts at: 2750 2854 3481 3982 4063 4398 6542 611
Size: 104 627 501 81 335 2144 839
Fragments arranged by size:
2144 839 794 627 501 335 308 253
204 191 169 154 104 81 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 5137 3229
Size: 1908 4862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 523 709 721 1234 2154 2497 2569 2601
Size: 135 186 12 513 920 343 72 32
Cuts at: 2601 2908 2938 3175 3224 3252 3541 3721 388
Size: 307 30 237 49 28 289 180 3437
Fragments arranged by size:
3437 920 513 343 307 289 237 186
180 135 72 49 32 30 28 12
Bpu10I CC'TnA_GC
Cuts at: 1083 1490 2534 2735 2770 3131 3290 3400 1083
Size: 407 1044 201 35 361 159 110 4453
Fragments arranged by size:
4453 1044 407 361 201 159 110 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4825 5317 5615 3946
Size: 879 492 298 5101
Fragments arranged by size:
5101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 958 1360 3277 6474 958
Size: 402 1917 3197 1254
Fragments arranged by size:
3197 1917 1254 402
BsaAI yAC'GTr
Cuts at: 3276 4123 3276
Size: 847 5923
BsaBI GATnn'nnATC
Cuts at: 6 4284 6321 6
Size: 4278 2037 455
Fragments arranged by size:
4278 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 558 801 3361 3372 3716 4201 4449 5151
Size: 376 243 2560 11 344 485 248 702
Cuts at: 5151 6647 182
Size: 1496 305
Fragments arranged by size:
2560 1496 702 485 376 344 305 248
243 11
BsaWI w'CCGG_w
Cuts at: 1152 3458 3959 4480 5158 5432 5579 5990 1152
Size: 2306 501 521 678 274 147 411 1932
Fragments arranged by size:
2306 1932 678 521 501 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 1093 1123 664
Size: 30 399 30 6311
Fragments arranged by size:
6311 399 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 1408 2494 3307 3718 1222
Size: 186 1086 813 411 4274
Fragments arranged by size:
4274 1086 813 411 186
BseYI C'CCAG_C
Cuts at: 297 711 1372 1554 2396 2728 3191 3452 4195
Size: 414 661 182 842 332 463 261 743
Cuts at: 4195 4953 5530 6026 297
Size: 758 577 496 1041
Fragments arranged by size:
1041 842 758 743 661 577 496 463
414 332 261 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 1664 2375 3001 3556 1339
Size: 325 711 626 555 4553
Fragments arranged by size:
4553 711 626 555 325
BsiEI CG_ry'CG
Cuts at: 104 130 4312 4358 5566 104
Size: 26 4182 46 1208 1308
Fragments arranged by size:
4182 1308 1208 46 26
BsiHKAI G_wGCw'C
Cuts at: 383 1577 1794 3031 3784 4562 4752 5544 383
Size: 1194 217 1237 753 778 190 792 1609
Fragments arranged by size:
1609 1237 1194 792 778 753 217 190
BsmI GAATG_Cn'
Cuts at: 2400 3760 2400
Size: 1360 5410
BsmBI CGTCTCn'nnnn_
Cuts at: 565 565
Size: 6770
Bsp1286I G_dGCh'C
Cuts at: 209 242 383 1060 1150 1334 1385 1577 1794
Size: 33 141 677 90 184 51 192 217
Cuts at: 1794 2496 2763 2851 2861 2886 3031 3102 3399
Size: 702 267 88 10 25 145 71 297
Cuts at: 3399 3478 3498 3599 3784 4395 4488 4562 4752
Size: 79 20 101 185 611 93 74 190
Cuts at: 4752 4814 5544 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 702 677 611 297 267 217
192 190 185 184 145 141 101 93
90 88 79 74 71 62 51 33
25 20 10
BspEI T'CCGG_A
Cuts at: 3458 5990 3458
Size: 2532 4238
BspHI T'CATG_A
Cuts at: 5946 5946
Size: 6770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 837 3820 4034 4336 4717 837
Size: 2983 214 302 381 2890
Fragments arranged by size:
2983 2890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 201 324 2892 3066 5062 5116 6600 6765
Size: 151 123 2568 174 1996 54 1484 165
Cuts at: 6765 50
Size: 55
Fragments arranged by size:
2568 1996 1484 174 165 151 123 55
54
BsrDI GCAATG_nn'
Cuts at: 4682 4682
Size: 6770
BsrFI r'CCGG_y
Cuts at: 4768 4949 5158 4768
Size: 181 209 6380
Fragments arranged by size:
6380 209 181
BsrGI T'GTAC_A
Cuts at: 338 338
Size: 6770
BssSI C'ACGA_G
Cuts at: 196 5041 5399 196
Size: 4845 358 1567
Fragments arranged by size:
4845 1567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3177 4147 3177
Size: 970 5800
BstBI TT'CG_AA
Cuts at: 5131 5131
Size: 6770
BstEII G'GTnAC_C
Cuts at: 2559 2559
Size: 6770
BstXI CCAn_nnnn'nTGG
Cuts at: 939 2049 939
Size: 1110 5660
BstYI r'GATC_y
Cuts at: 1 782 2334 2951 3795 4262 4285 4620 4866
Size: 781 1552 617 844 467 23 335 246
Cuts at: 4866 5878 5964 5976 6322 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1552 1012 844 781 617 467 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 244 287 1403 1566 2420 2520 3263 3417 244
Size: 43 1116 163 854 100 743 154 3597
Fragments arranged by size:
3597 1116 854 743 163 154 100 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 643 4720 4901 5064 643
Size: 4077 181 163 2349
Fragments arranged by size:
4077 2349 181 163
BtsI GCAGTG_nn'
Cuts at: 957 1283 2820 3670 4013 957
Size: 326 1537 850 343 3714
Fragments arranged by size:
3714 1537 850 343 326
ClaI AT'CG_AT
Cuts at: 524 524
Size: 6770
DraI TTT'AAA
Cuts at: 3769 3769
Size: 6770
DraIII CAC_nnn'GTG
Cuts at: 2758 2780 2758
Size: 22 6748
DrdI GACnn_nn'nnGTC
Cuts at: 469 1230 4476 5334 469
Size: 761 3246 858 1905
Fragments arranged by size:
3246 1905 858 761
EaeI y'GGCC_r
Cuts at: 127 929 1608 2417 2523 2957 3749 4355 4529
Size: 802 679 809 106 434 792 606 174
Cuts at: 4529 4920 4947 6292 127
Size: 391 27 1345 605
Fragments arranged by size:
1345 809 802 792 679 606 605 434
391 174 106 27
EagI C'GGCC_G
Cuts at: 127 4355 127
Size: 4228 2542
EarI CTCTTCn'nnn_
Cuts at: 3668 4793 5003 3668
Size: 1125 210 5435
Fragments arranged by size:
5435 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4978 5288 5434 6580 4978
Size: 310 146 1146 5168
Fragments arranged by size:
5168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3782 3782
Size: 6770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 370 388 523 709 721 940 1234 1235
Size: 160 18 135 186 12 219 294 1
Cuts at: 1235 1481 1706 2154 2497 2569 2601 2640 2908
Size: 246 225 448 343 72 32 39 268
Cuts at: 2908 2938 3175 3224 3252 3364 3541 3721 4595
Size: 30 237 49 28 112 177 180 874
Cuts at: 4595 5027 5774 6042 210
Size: 432 747 268 938
Fragments arranged by size:
938 874 747 448 432 343 294 268
268 246 237 225 219 186 180 177
160 135 112 72 49 39 32 30
28 18 12 1
EcoNI CCTnn'n_nnAGG
Cuts at: 640 991 1356 2479 2551 640
Size: 351 365 1123 72 4859
Fragments arranged by size:
4859 1123 365 351 72
EcoO109I rG'GnC_Cy
Cuts at: 205 238 239 357 605 924 972 1310 1382
Size: 33 1 118 248 319 48 338 72
Cuts at: 1382 1534 1735 1864 2492 2493 2544 2858 2883
Size: 152 201 129 628 1 51 314 25
Cuts at: 2883 3188 3199 3348 3396 3475 3494 3495 3909
Size: 305 11 149 48 79 19 1 414
Cuts at: 3909 205
Size: 3066
Fragments arranged by size:
3066 628 414 338 319 314 305 248
201 152 149 129 118 79 72 51
48 48 33 25 19 11 1 1
1
EcoRI G'AATT_C
Cuts at: 432 3774 432
Size: 3342 3428
EcoRV GAT'ATC
Cuts at: 1011 1011
Size: 6770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4789 4789
Size: 6770
FspI TGC'GCA
Cuts at: 4551 4551
Size: 6770
HaeII r_GCGC'y
Cuts at: 138 152 349 810 1128 3364 4204 4452 5474
Size: 14 197 461 318 2236 840 248 1022
Cuts at: 5474 6453 138
Size: 979 455
Fragments arranged by size:
2236 1022 979 840 461 455 318 248
197 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 666 698 1066 1098 1147 1179 1246 1278 2580
Size: 32 368 32 49 32 67 32 1302
Cuts at: 2580 2612 3667 3699 4612 4642 4644 4674 666
Size: 32 1055 32 913 30 2 30 2762
Fragments arranged by size:
2762 1302 1055 913 368 67 49 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1101 3809 1101
Size: 2708 4062
KasI G'GCGC_C
Cuts at: 3360 4200 4448 3360
Size: 840 248 5682
Fragments arranged by size:
5682 840 248
KpnI G_GTAC'C
Cuts at: 1028 3790 1028
Size: 2762 4008
MluI A'CGCG_T
Cuts at: 6203 6203
Size: 6770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1423 5441 5625 1423
Size: 4018 184 2568
Fragments arranged by size:
4018 2568 184
MscI TGG'CCA
Cuts at: 931 1610 2419 2525 3751 4531 931
Size: 679 809 106 1226 780 3170
Fragments arranged by size:
3170 1226 809 780 679 106
MslI CAynn'nnrTG
Cuts at: 1460 2632 4759 4886 1460
Size: 1172 2127 127 3344
Fragments arranged by size:
3344 2127 1172 127
MspA1I CmG'CkG
Cuts at: 555 711 1087 3044 3289 3519 3881 4195 4555
Size: 156 376 1957 245 230 362 314 360
Cuts at: 4555 5568 5813 555
Size: 1013 245 1512
Fragments arranged by size:
1957 1512 1013 376 362 360 314 245
245 230 156
NaeI GCC'GGC
Cuts at: 4951 4951
Size: 6770
NarI GG'CG_CC
Cuts at: 3361 4201 4449 3361
Size: 840 248 5682
Fragments arranged by size:
5682 840 248
NcoI C'CATG_G
Cuts at: 1566 2420 3263 3417 1566
Size: 854 843 154 4919
Fragments arranged by size:
4919 854 843 154
NgoMIV G'CCGG_C
Cuts at: 4949 4949
Size: 6770
NotI GC'GGCC_GC
Cuts at: 127 127
Size: 6770
NruI TCG'CGA
Cuts at: 662 662
Size: 6770
NspI r_CATG'y
Cuts at: 681 1194 1432 2644 3823 4323 5230 681
Size: 513 238 1212 1179 500 907 2221
Fragments arranged by size:
2221 1212 1179 907 513 500 238
PasI CC'CwG_GG
Cuts at: 3165 3165
Size: 6770
PciI A'CATG_T
Cuts at: 5226 5226
Size: 6770
PflMI CCAn_nnn'nTGG
Cuts at: 999 2976 3202 999
Size: 1977 226 4567
Fragments arranged by size:
4567 1977 226
PfoI T'CCnGG_A
Cuts at: 274 563 3152 3485 3509 274
Size: 289 2589 333 24 3535
Fragments arranged by size:
3535 2589 333 289 24
PmeI GTTT'AAAC
Cuts at: 3769 3769
Size: 6770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5909 5941 5909
Size: 32 6738
PpuMI rG'GwC_Cy
Cuts at: 605 972 1534 605
Size: 367 562 5841
Fragments arranged by size:
5841 562 367
PshAI GACnn'nnGTC
Cuts at: 2437 2437
Size: 6770
PsiI TTA'TAA
Cuts at: 2128 2128
Size: 6770
PspOMI G'GGCC_C
Cuts at: 205 238 1381 2492 2857 2882 3395 3474 3494
Size: 33 1143 1111 365 25 513 79 20
Cuts at: 3494 205
Size: 3481
Fragments arranged by size:
3481 1143 1111 513 365 79 33 25
20
PspXI vC'TCGA_Gb
Cuts at: 3542 3542
Size: 6770
PstI C_TGCA'G
Cuts at: 2543 2827 3817 4502 2543
Size: 284 990 685 4811
Fragments arranged by size:
4811 990 685 284
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6770
PvuII CAG'CTG
Cuts at: 711 1087 3044 3519 4195 4555 711
Size: 376 1957 475 676 360 2926
Fragments arranged by size:
2926 1957 676 475 376 360
RsrII CG'GwC_CG
Cuts at: 4965 4965
Size: 6770
SacI G_AGCT'C
Cuts at: 3784 3784
Size: 6770
SalI G'TCGA_C
Cuts at: 3807 3807
Size: 6770
SanDI GG'GwC_CC
Cuts at: 605 605
Size: 6770
SapI GCTCTTCn'nnn_
Cuts at: 4793 5003 4793
Size: 210 6560
SbfI CC_TGCA'GG
Cuts at: 3817 3817
Size: 6770
ScaI AGT'ACT
Cuts at: 2915 6287 2915
Size: 3372 3398
SexAI A'CCwGG_T
Cuts at: 332 332
Size: 6770
SfcI C'TryA_G
Cuts at: 33 1137 1437 1661 1751 1764 1817 1859 2223
Size: 1104 300 224 90 13 53 42 364
Cuts at: 2223 2539 2823 3658 3813 4498 5491 5682 33
Size: 316 284 835 155 685 993 191 1121
Fragments arranged by size:
1121 1104 993 835 685 364 316 300
284 224 191 155 90 53 42 13
SfoI GGC'GCC
Cuts at: 3362 4202 4450 3362
Size: 840 248 5682
Fragments arranged by size:
5682 840 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6770
SmaI CCC'GGG
Cuts at: 154 611 628 3792 154
Size: 457 17 3164 3132
Fragments arranged by size:
3164 3132 457 17
SmlI C'TyrA_G
Cuts at: 3542 3925 4840 5332 5594 3542
Size: 383 915 492 262 4718
Fragments arranged by size:
4718 915 492 383 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6770
SphI G_CATG'C
Cuts at: 1432 2644 3823 4323 1432
Size: 1212 1179 500 3879
Fragments arranged by size:
3879 1212 1179 500
StuI AGG'CCT
Cuts at: 1108 1362 2709 3271 1108
Size: 254 1347 562 4607
Fragments arranged by size:
4607 1347 562 254
StyI C'CwwG_G
Cuts at: 305 968 1020 1306 1385 1484 1566 2420 2645
Size: 663 52 286 79 99 82 854 225
Cuts at: 2645 2801 3248 3263 3417 3564 3904 4258 305
Size: 156 447 15 154 147 340 354 2817
Fragments arranged by size:
2817 854 663 447 354 340 286 225
156 154 147 99 82 79 52 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 311 1568 1579 1843 1927 1990 2011 2095 2116
Size: 1257 11 264 84 63 21 84 21
Cuts at: 2116 2300 2769 3466 4353 4486 4945 5154 311
Size: 184 469 697 887 133 459 209 1927
Fragments arranged by size:
1927 1257 887 697 469 459 264 209
184 133 84 84 63 21 21 11
TatI w'GTAC_w
Cuts at: 338 665 2913 6285 338
Size: 327 2248 3372 823
Fragments arranged by size:
3372 2248 823 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 664 696 833 865 2016 2048 2766 2798 6287
Size: 32 137 32 1151 32 718 32 3489
Cuts at: 6287 6319 664
Size: 32 1115
Fragments arranged by size:
3489 1151 1115 718 137 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2594 4567 2594
Size: 1973 4797
XbaI T'CTAG_A
Cuts at: 2151 3801 2151
Size: 1650 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3199 4265 3199
Size: 1066 5704
XhoI C'TCGA_G
Cuts at: 3542 3542
Size: 6770
XmaI C'CCGG_G
Cuts at: 152 609 626 3790 152
Size: 457 17 3164 3132
Fragments arranged by size:
3164 3132 457 17
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BfrBI BmtI BsiWI
BssHII BstZ17I Bsu36I CspCI FseI FspAI HindIII HpaI
MfeI NdeI NheI NsiI PacI PmlI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI