Restriction map for
pF1KA1909
(Circular) (Six-base) MAPSORT of: pF1KA1909.seq Check: 6371 from: 1 to: 6926
pF1KA1909 6926 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 663 1798 2496 663
Size: 1135 698 5093
Fragments arranged by size:
5093 1135 698
AatII G_ACGT'C
Cuts at: 686 3236 686
Size: 2550 4376
AccI GT'mk_AC
Cuts at: 3236 3964 3236
Size: 728 6198
Acc65I G'GTAC_C
Cuts at: 3942 3942
Size: 6926
AclI AA'CG_TT
Cuts at: 6766 6766
Size: 6926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1843 2343 4751 5183 5536 6198 1072
Size: 771 500 2408 432 353 662 1800
Fragments arranged by size:
2408 1800 771 662 500 432 353
AfeI AGC'GCT
Cuts at: 2087 2722 2087
Size: 635 6291
AflIII A'CryG_T
Cuts at: 4278 6078 6359 4278
Size: 1800 281 4845
Fragments arranged by size:
4845 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 275 2148 275
Size: 1873 5053
AleI CACnn'nnGTG
Cuts at: 3628 3628
Size: 6926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1991 2837 3073 1991
Size: 846 236 5844
Fragments arranged by size:
5844 846 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 6894
AlwNI CAG_nnn'CTG
Cuts at: 322 678 696 1152 1479 2238 2955 2997 3797
Size: 356 18 456 327 759 717 42 800
Cuts at: 3797 3818 4231 5669 322
Size: 21 413 1438 1579
Fragments arranged by size:
1579 1438 800 759 717 456 413 356
327 42 21 18
ApaI G_GGCC'C
Cuts at: 390 1709 2122 390
Size: 1319 413 5194
Fragments arranged by size:
5194 1319 413
ApaLI G'TGCA_C
Cuts at: 1060 1438 5764 1060
Size: 378 4326 2222
Fragments arranged by size:
4326 2222 378
ApoI r'AATT_y
Cuts at: 1066 1200 3093 3930 6279 6535 1066
Size: 134 1893 837 2349 256 1457
Fragments arranged by size:
2349 1893 1457 837 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 6926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
AvaI C'yCGr_G
Cuts at: 157 251 616 731 1801 2469 3768 3946 157
Size: 94 365 115 1070 668 1299 178 3137
Fragments arranged by size:
3137 1299 1070 668 365 178 115 94
AvrII C'CTAG_G
Cuts at: 447 447
Size: 6926
BamHI G'GATC_C
Cuts at: 3410 3951 3410
Size: 541 6385
BanI G'GyrC_C
Cuts at: 366 663 1336 1997 2041 3859 3942 4356 4604
Size: 297 673 661 44 1818 83 414 248
Cuts at: 4604 4639 366
Size: 35 2653
Fragments arranged by size:
2653 1818 673 661 414 297 248 83
44 35
BanII G_rGCy'C
Cuts at: 390 1048 1709 1960 2114 2122 2228 2434 2477
Size: 658 661 251 154 8 106 206 43
Cuts at: 2477 3008 3549 3784 3888 3940 4970 390
Size: 531 541 235 104 52 1030 2346
Fragments arranged by size:
2346 1030 661 658 541 531 251 235
206 154 106 104 52 43 8
BbeI G_GCGC'C
Cuts at: 4360 4608 4360
Size: 248 6678
BbsI GAAGACnn'nnnn_
Cuts at: 521 1307 1385 2349 3191 521
Size: 786 78 964 842 4256
Fragments arranged by size:
4256 964 842 786 78
BbvCI CC'TCA_GC
Cuts at: 531 1354 2306 2486 531
Size: 823 952 180 4971
Fragments arranged by size:
4971 952 823 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 580 614 580
Size: 34 6892
BciVI GTATCCnnnnn_n'
Cuts at: 2575 4824 5880 6926 2575
Size: 2249 1056 1046 2575
Fragments arranged by size:
2575 2249 1056 1046
BclI T'GATC_A
Cuts at: 4446 4446
Size: 6926
BglI GCCn_nnn'nGGC
Cuts at: 603 1984 2188 603
Size: 1381 204 5341
Fragments arranged by size:
5341 1381 204
BglII A'GATC_T
Cuts at: 346 4441 6478 346
Size: 4095 2037 794
Fragments arranged by size:
4095 2037 794
BlpI GC'TnA_GC
Cuts at: 809 2830 3901 4038 809
Size: 2021 1071 137 3697
Fragments arranged by size:
3697 2021 1071 137
Bme1580I G_kGCm'C
Cuts at: 369 390 668 1064 1339 1442 1709 1980 2122
Size: 21 278 396 275 103 267 271 142
Cuts at: 2122 3601 3862 4551 4644 5768 369
Size: 1479 261 689 93 1124 1527
Fragments arranged by size:
1527 1479 1124 689 396 278 275 271
267 261 142 103 93 21
BmgBI CAC'GTC
Cuts at: 3090 3090
Size: 6926
BmrI ACTGGGnnnn_n'
Cuts at: 218 478 4138 4219 4554 6698 218
Size: 260 3660 81 335 2144 446
Fragments arranged by size:
3660 2144 446 335 260 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 1794 2223 5293 258
Size: 1536 429 3070 1891
Fragments arranged by size:
3070 1891 1536 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 363 414 1348 1417 1456 1501 2218 2392
Size: 157 51 934 69 39 45 717 174
Cuts at: 2392 2737 2932 3738 206
Size: 345 195 806 3394
Fragments arranged by size:
3394 934 806 717 345 195 174 157
69 51 45 39
Bpu10I CC'TnA_GC
Cuts at: 531 1354 1609 2306 2486 2577 531
Size: 823 255 697 180 91 4880
Fragments arranged by size:
4880 823 697 255 180 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2944 3324 4102 4981 5695 5993 2944
Size: 380 778 879 714 298 3877
Fragments arranged by size:
3877 879 778 714 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 251 728 3503 6630 251
Size: 477 2775 3127 547
Fragments arranged by size:
3127 2775 547 477
BsaAI yAC'GTr
Cuts at: 2790 4279 2790
Size: 1489 5437
BsaBI GATnn'nnATC
Cuts at: 6 4440 6477 6
Size: 4434 2037 455
Fragments arranged by size:
4434 2037 455
BsaHI Gr'CG_yC
Cuts at: 683 1501 3071 3233 4357 4605 5307 6803 683
Size: 818 1570 162 1124 248 702 1496 806
Fragments arranged by size:
1570 1496 1124 818 806 702 248 162
BsaWI w'CCGG_w
Cuts at: 3512 4115 4636 5314 5725 5872 6146 3512
Size: 603 521 678 411 147 274 4292
Fragments arranged by size:
4292 678 603 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1486 1516 2138 2168 2881 2911 1486
Size: 30 622 30 713 30 5501
Fragments arranged by size:
5501 713 622 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 1371 1447 1722 1770 2007 2146 2503 3627
Size: 1208 76 275 48 237 139 357 1124
Cuts at: 3627 3687 3708 163
Size: 60 21 3381
Fragments arranged by size:
3381 1208 1124 357 275 237 139 76
60 48 21
BseYI C'CCAG_C
Cuts at: 301 577 655 1933 2020 2997 3735 3759 4351
Size: 276 78 1278 87 977 738 24 592
Cuts at: 4351 5109 5774 6182 301
Size: 758 665 408 1045
Fragments arranged by size:
1278 1045 977 758 738 665 592 408
276 87 78 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 778 1656 2938 3548 3578 486
Size: 292 878 1282 610 30 3834
Fragments arranged by size:
3834 1282 878 610 292 30
BsiEI CG_ry'CG
Cuts at: 104 1422 3266 3573 4468 4514 5744 104
Size: 1318 1844 307 895 46 1230 1286
Fragments arranged by size:
1844 1318 1286 1230 895 307 46
BsiHKAI G_wGCw'C
Cuts at: 1048 1064 1442 1487 2136 3008 3940 4718 4908
Size: 16 378 45 649 872 932 778 190
Cuts at: 4908 5768 1048
Size: 860 2206
Fragments arranged by size:
2206 932 872 860 778 649 378 190
45 16
BsmBI CGTCTCn'nnnn_
Cuts at: 924 1270 1474 1678 3847 924
Size: 346 204 204 2169 4003
Fragments arranged by size:
4003 2169 346 204 204
Bsp1286I G_dGCh'C
Cuts at: 369 390 668 1048 1064 1339 1442 1487 1709
Size: 21 278 380 16 275 103 45 222
Cuts at: 1709 1960 1980 2114 2122 2136 2228 2434 2477
Size: 251 20 134 8 14 92 206 43
Cuts at: 2477 3008 3549 3601 3784 3862 3888 3940 4551
Size: 531 541 52 183 78 26 52 611
Cuts at: 4551 4644 4718 4908 4970 5768 369
Size: 93 74 190 62 798 1527
Fragments arranged by size:
1527 798 611 541 531 380 278 275
251 222 206 190 183 134 103 93
92 78 74 62 52 52 45 43
26 21 20 16 14 8
BspHI T'CATG_A
Cuts at: 5358 5358
Size: 6926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 663 750 1798 2061 2496 2912 2963 3065 3976
Size: 87 1048 263 435 416 51 102 911
Cuts at: 3976 4190 4492 4873 663
Size: 214 302 381 2716
Fragments arranged by size:
2716 1048 911 435 416 381 302 263
214 102 87 51
BsrBI CCG'CTC
Cuts at: 50 1667 5218 5272 6756 6921 50
Size: 1617 3551 54 1484 165 55
Fragments arranged by size:
3551 1617 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 3057 4838 564
Size: 2493 1781 2652
Fragments arranged by size:
2652 2493 1781
BsrFI r'CCGG_y
Cuts at: 4924 5105 4924
Size: 181 6745
BsrGI T'GTAC_A
Cuts at: 3242 3242
Size: 6926
BssSI C'ACGA_G
Cuts at: 5197 5905 5197
Size: 708 6218
BstAPI GCAn_nnn'nTGC
Cuts at: 4303 4303
Size: 6926
BstBI TT'CG_AA
Cuts at: 5287 5287
Size: 6926
BstEII G'GTnAC_C
Cuts at: 2376 2376
Size: 6926
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 6926
BstYI r'GATC_y
Cuts at: 1 346 1133 3410 3951 4418 4441 4776 5022
Size: 345 787 2277 541 467 23 335 246
Cuts at: 5022 5328 5340 5426 6478 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2277 1052 787 541 467 449 345 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2158 2158
Size: 6926
BtgI C'CryG_G
Cuts at: 107 1122 1616 1739 1846 2186 2744 2838 3065
Size: 1015 494 123 107 340 558 94 227
Cuts at: 3065 3078 3467 107
Size: 13 389 3566
Fragments arranged by size:
3566 1015 558 494 389 340 227 123
107 94 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4876 5057 5220 4876
Size: 181 163 6582
Fragments arranged by size:
6582 181 163
BtsI GCAGTG_nn'
Cuts at: 758 1306 1823 2730 3536 3566 3878 4169 758
Size: 548 517 907 806 30 312 291 3515
Fragments arranged by size:
3515 907 806 548 517 312 291 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 610 645 1849 1884 610
Size: 35 1204 35 5652
Fragments arranged by size:
5652 1204 35 35
DraI TTT'AAA
Cuts at: 3925 3925
Size: 6926
DrdI GACnn_nn'nnGTC
Cuts at: 1668 3589 4632 5976 1668
Size: 1921 1043 1344 2618
Fragments arranged by size:
2618 1921 1344 1043
EaeI y'GGCC_r
Cuts at: 631 1652 2240 2537 2741 2927 3062 3263 4511
Size: 1021 588 297 204 186 135 201 1248
Cuts at: 4511 4685 5076 5103 6448 631
Size: 174 391 27 1345 1109
Fragments arranged by size:
1345 1248 1109 1021 588 391 297 204
201 186 174 135 27
EagI C'GGCC_G
Cuts at: 3263 4511 3263
Size: 1248 5678
EarI CTCTTCn'nnn_
Cuts at: 341 1358 1898 2765 3705 4949 5159 341
Size: 1017 540 867 940 1244 210 2108
Fragments arranged by size:
2108 1244 1017 940 867 540 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 345 5134 5876 6022 6736 345
Size: 4789 742 146 714 535
Fragments arranged by size:
4789 742 714 535 146
EcoICRI GAG'CTC
Cuts at: 1046 3006 3938 1046
Size: 1960 932 4034
Fragments arranged by size:
4034 1960 932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 363 414 1072 1348 1417 1456 1501 1843
Size: 157 51 658 276 69 39 45 342
Cuts at: 1843 2218 2343 2392 2737 2932 3738 4751 5183
Size: 375 125 49 345 195 806 1013 432
Cuts at: 5183 5536 6198 206
Size: 353 662 934
Fragments arranged by size:
1013 934 806 662 658 432 375 353
345 342 276 195 157 125 69 51
49 45 39
EcoNI CCTnn'n_nnAGG
Cuts at: 360 1915 2959 2983 360
Size: 1555 1044 24 4303
Fragments arranged by size:
4303 1555 1044 24
EcoO109I rG'GnC_Cy
Cuts at: 153 162 198 227 386 467 493 728 864
Size: 9 36 29 159 81 26 235 136
Cuts at: 864 927 1605 1705 1706 1908 2142 2163 2367
Size: 63 678 100 1 202 234 21 204
Cuts at: 2367 2634 2952 3576 4065 153
Size: 267 318 624 489 3014
Fragments arranged by size:
3014 678 624 489 318 267 235 234
204 202 159 136 100 81 63 36
29 26 21 9 1
EcoRI G'AATT_C
Cuts at: 1200 3930 1200
Size: 2730 4196
EcoRV GAT'ATC
Cuts at: 3810 3810
Size: 6926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 538 1927 3270 4945 538
Size: 1389 1343 1675 2519
Fragments arranged by size:
2519 1675 1389 1343
FspI TGC'GCA
Cuts at: 4707 4707
Size: 6926
HaeII r_GCGC'y
Cuts at: 1616 2089 2724 4360 4608 5838 6609 1616
Size: 473 635 1636 248 1230 771 1933
Fragments arranged by size:
1933 1636 1230 771 635 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 979 1011 1486 1513 1518 1545 1746
Size: 32 686 32 475 27 5 27 201
Cuts at: 1746 1778 2134 2166 2439 2471 2881 2913 3806
Size: 32 356 32 273 32 410 32 893
Cuts at: 3806 3838 4768 4798 4800 4830 261
Size: 32 930 30 2 30 2357
Fragments arranged by size:
2357 930 893 686 475 410 356 273
201 32 32 32 32 32 32 32
30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 1074 3965 1074
Size: 2891 4035
HindIII A'AGCT_T
Cuts at: 1288 1288
Size: 6926
KasI G'GCGC_C
Cuts at: 4356 4604 4356
Size: 248 6678
KpnI G_GTAC'C
Cuts at: 3946 3946
Size: 6926
MluI A'CGCG_T
Cuts at: 6359 6359
Size: 6926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2798 5685 5869 2798
Size: 2887 184 3855
Fragments arranged by size:
3855 2887 184
MscI TGG'CCA
Cuts at: 633 1654 2242 2929 3064 4687 633
Size: 1021 588 687 135 1623 2872
Fragments arranged by size:
2872 1623 1021 687 588 135
MslI CAynn'nnrTG
Cuts at: 1973 2351 3050 3628 4915 5042 1973
Size: 378 699 578 1287 127 3857
Fragments arranged by size:
3857 1287 699 578 378 127
MspA1I CmG'CkG
Cuts at: 581 907 1079 1086 1479 1551 1697 2412 2868
Size: 326 172 7 393 72 146 715 456
Cuts at: 2868 2972 3039 3080 3114 3628 4037 4351 4711
Size: 104 67 41 34 514 409 314 360
Cuts at: 4711 5495 5740 581
Size: 784 245 1767
Fragments arranged by size:
1767 784 715 514 456 409 393 360
326 314 245 172 146 104 72 67
41 34 7
NaeI GCC'GGC
Cuts at: 5107 5107
Size: 6926
NarI GG'CG_CC
Cuts at: 4357 4605 4357
Size: 248 6678
NcoI C'CATG_G
Cuts at: 107 1122 2838 3065 3467 107
Size: 1015 1716 227 402 3566
Fragments arranged by size:
3566 1716 1015 402 227
NgoMIV G'CCGG_C
Cuts at: 5105 5105
Size: 6926
NspI r_CATG'y
Cuts at: 2425 3172 3979 4479 6082 2425
Size: 747 807 500 1603 3269
Fragments arranged by size:
3269 1603 807 747 500
PasI CC'CwG_GG
Cuts at: 135 724 1836 2115 2122 2324 2631 2985 135
Size: 589 1112 279 7 202 307 354 4076
Fragments arranged by size:
4076 1112 589 354 307 279 202 7
PciI A'CATG_T
Cuts at: 6078 6078
Size: 6926
PflMI CCAn_nnn'nTGG
Cuts at: 1479 2238 3216 3459 1479
Size: 759 978 243 4946
Fragments arranged by size:
4946 978 759 243
PfoI T'CCnGG_A
Cuts at: 315 860 1295 1490 1508 1589 2708 2948 315
Size: 545 435 195 18 81 1119 240 4293
Fragments arranged by size:
4293 1119 545 435 240 195 81 18
PmeI GTTT'AAAC
Cuts at: 3925 3925
Size: 6926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 5372
Size: 32 6894
PpuMI rG'GwC_Cy
Cuts at: 162 198 227 467 493 728 864 2142 2952
Size: 36 29 240 26 235 136 1278 810
Cuts at: 2952 3576 162
Size: 624 3512
Fragments arranged by size:
3512 1278 810 624 240 235 136 36
29 26
PshAI GACnn'nnGTC
Cuts at: 686 3397 686
Size: 2711 4215
PspOMI G'GGCC_C
Cuts at: 386 1705 2118 386
Size: 1319 413 5194
Fragments arranged by size:
5194 1319 413
PstI C_TGCA'G
Cuts at: 175 327 399 704 1791 1877 3873 3973 4658
Size: 152 72 305 1087 86 1996 100 685
Cuts at: 4658 175
Size: 2443
Fragments arranged by size:
2443 1996 1087 685 305 152 100 86
72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6926
PvuII CAG'CTG
Cuts at: 907 1079 1086 1479 1551 1697 2972 3039 3114
Size: 172 7 393 72 146 1275 67 75
Cuts at: 3114 4351 4711 907
Size: 1237 360 3122
Fragments arranged by size:
3122 1275 1237 393 360 172 146 75
72 67 7
RsrII CG'GwC_CG
Cuts at: 5121 5121
Size: 6926
SacI G_AGCT'C
Cuts at: 1048 3008 3940 1048
Size: 1960 932 4034
Fragments arranged by size:
4034 1960 932
SacII CC_GC'GG
Cuts at: 3081 3081
Size: 6926
SalI G'TCGA_C
Cuts at: 3963 3963
Size: 6926
SanDI GG'GwC_CC
Cuts at: 227 467 493 728 2142 3576 227
Size: 240 26 235 1414 1434 3577
Fragments arranged by size:
3577 1434 1414 240 235 26
SapI GCTCTTCn'nnn_
Cuts at: 1358 2765 4949 5159 1358
Size: 1407 2184 210 3125
Fragments arranged by size:
3125 2184 1407 210
SbfI CC_TGCA'GG
Cuts at: 3973 3973
Size: 6926
ScaI AGT'ACT
Cuts at: 6443 6443
Size: 6926
SfcI C'TryA_G
Cuts at: 33 171 323 395 700 1116 1195 1546 1787
Size: 138 152 72 305 416 79 351 241
Cuts at: 1787 1873 2387 2796 3869 3969 4654 5622 5813
Size: 86 514 409 1073 100 685 968 191
Cuts at: 5813 33
Size: 1146
Fragments arranged by size:
1146 1073 968 685 514 416 409 351
305 241 191 152 138 100 86 79
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2188 2188
Size: 6926
SfoI GGC'GCC
Cuts at: 4358 4606 4358
Size: 248 6678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
SmaI CCC'GGG
Cuts at: 253 1803 3770 3948 253
Size: 1550 1967 178 3231
Fragments arranged by size:
3231 1967 1550 178
SmlI C'TyrA_G
Cuts at: 2959 3303 4081 4996 5710 5972 2959
Size: 344 778 915 714 262 3913
Fragments arranged by size:
3913 915 778 714 344 262
SpeI A'CTAG_T
Cuts at: 67 2350 67
Size: 2283 4643
SphI G_CATG'C
Cuts at: 2425 3979 4479 2425
Size: 1554 500 4872
Fragments arranged by size:
4872 1554 500
StuI AGG'CCT
Cuts at: 435 1008 1795 435
Size: 573 787 5566
Fragments arranged by size:
5566 787 573
StyI C'CwwG_G
Cuts at: 107 447 1122 2738 2838 3065 3467 4060 4414
Size: 340 675 1616 100 227 402 593 354
Cuts at: 4414 107
Size: 2619
Fragments arranged by size:
2619 1616 675 593 402 354 340 227
100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 341 384 1237 1436 2245 3409 3877 4509
Size: 26 43 853 199 809 1164 468 632
Cuts at: 4509 4642 5101 5310 315
Size: 133 459 209 1931
Fragments arranged by size:
1931 1164 853 809 632 468 459 209
199 133 43 26
TatI w'GTAC_w
Cuts at: 830 2564 3242 6441 830
Size: 1734 678 3199 1315
Fragments arranged by size:
3199 1734 1315 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 2000 2032 2138 2170 6443 6475 446
Size: 32 1522 32 106 32 4273 32 897
Fragments arranged by size:
4273 1522 897 106 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3574 4723 3574
Size: 1149 5777
XbaI T'CTAG_A
Cuts at: 279 3957 279
Size: 3678 3248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 198 675 2193 2235 2976 4421 198
Size: 477 1518 42 741 1445 2703
Fragments arranged by size:
2703 1518 1445 741 477 42
XmaI C'CCGG_G
Cuts at: 251 1801 3768 3946 251
Size: 1550 1967 178 3231
Fragments arranged by size:
3231 1967 1550 178
XmnI GAAnn'nnTTC
Cuts at: 518 1204 518
Size: 686 6240
ZraI GAC'GTC
Cuts at: 684 3234 684
Size: 2550 4376
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AscI BaeI BfrBI BmtI BsiWI BsmI
BspEI BssHII BstZ17I ClaI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI