Restriction map for
pF1KA1878
(Circular) (Six-base) MAPSORT of: pF1KA1878.seq Check: 1427 from: 1 to: 4430
pF1KA1878 4430 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2687 3040 3702 2255
Size: 432 353 662 2983
Fragments arranged by size:
2983 662 432 353
AfeI AGC'GCT
Cuts at: 715 715
Size: 4430
AflIII A'CryG_T
Cuts at: 1782 3582 3863 1782
Size: 1800 281 2349
Fragments arranged by size:
2349 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 665 665
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 367 1735 3173 367
Size: 1368 1438 1624
Fragments arranged by size:
1624 1438 1368
ApaI G_GGCC'C
Cuts at: 403 424 1354 403
Size: 21 930 3479
Fragments arranged by size:
3479 930 21
ApaLI G'TGCA_C
Cuts at: 3268 3268
Size: 4430
ApoI r'AATT_y
Cuts at: 1018 1434 3783 4039 1018
Size: 416 2349 256 1409
Fragments arranged by size:
2349 1409 416 256
AscI GG'CGCG_CC
Cuts at: 942 942
Size: 4430
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 225 347 423 505 679 743 925 1353 1450
Size: 122 76 82 174 64 182 428 97
Cuts at: 1450 225
Size: 3205
Fragments arranged by size:
3205 428 182 174 122 97 82 76
64
BamHI G'GATC_C
Cuts at: 626 1455 626
Size: 829 3601
BanI G'GyrC_C
Cuts at: 229 669 684 1446 1860 2108 2143 229
Size: 440 15 762 414 248 35 2516
Fragments arranged by size:
2516 762 440 414 248 35 15
BanII G_rGCy'C
Cuts at: 403 424 436 512 1354 1444 2474 403
Size: 21 12 76 842 90 1030 2359
Fragments arranged by size:
2359 1030 842 90 76 21 12
BbeI G_GCGC'C
Cuts at: 233 673 688 1864 2112 233
Size: 440 15 1176 248 2551
Fragments arranged by size:
2551 1176 440 248 15
BbvCI CC'TCA_GC
Cuts at: 737 1269 1412 737
Size: 532 143 3755
Fragments arranged by size:
3755 532 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 353 319
Size: 34 4396
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3384 4430 2328
Size: 1056 1046 2328
Fragments arranged by size:
2328 1056 1046
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglI GCCn_nnn'nGGC
Cuts at: 661 739 793 661
Size: 78 54 4298
Fragments arranged by size:
4298 78 54
BglII A'GATC_T
Cuts at: 644 1945 3982 644
Size: 1301 2037 1092
Fragments arranged by size:
2037 1301 1092
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 403 424 875 1354 2055 2148 3272 403
Size: 21 451 479 701 93 1124 1561
Fragments arranged by size:
1561 1124 701 479 451 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 564 1065 1642 1723 2058 4202 564
Size: 501 577 81 335 2144 792
Fragments arranged by size:
2144 792 577 501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4430
Bpu10I CC'TnA_GC
Cuts at: 737 1269 1412 737
Size: 532 143 3755
Fragments arranged by size:
3755 532 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2485 3199 3497 1606
Size: 879 714 298 2539
Fragments arranged by size:
2539 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 1099 4134 559
Size: 540 3035 855
Fragments arranged by size:
3035 855 540
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 230 664 670 685 796 883 956 1861 2109
Size: 434 6 15 111 87 73 905 248
Cuts at: 2109 2811 4307 230
Size: 702 1496 353
Fragments arranged by size:
1496 905 702 434 353 248 111 87
73 15 6
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3229 3376 3650 1619
Size: 521 678 411 147 274 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 526
Size: 30 4400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 994 994
Size: 4430
BseYI C'CCAG_C
Cuts at: 511 1855 2613 3278 3686 511
Size: 1344 758 665 408 1255
Fragments arranged by size:
1344 1255 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 661 745 1013 517
Size: 144 84 268 3934
Fragments arranged by size:
3934 268 144 84
BsiEI CG_ry'CG
Cuts at: 104 408 523 700 788 1972 2018 3248 104
Size: 304 115 177 88 1184 46 1230 1286
Fragments arranged by size:
1286 1230 1184 304 177 115 88 46
BsiHKAI G_wGCw'C
Cuts at: 224 1122 1444 2222 2412 3272 224
Size: 898 322 778 190 860 1382
Fragments arranged by size:
1382 898 860 778 322 190
BsmBI CGTCTCn'nnnn_
Cuts at: 304 304
Size: 4430
Bsp1286I G_dGCh'C
Cuts at: 224 403 424 436 512 875 1122 1354 1444
Size: 179 21 12 76 363 247 232 90
Cuts at: 1444 2055 2148 2222 2412 2474 3272 224
Size: 611 93 74 190 62 798 1382
Fragments arranged by size:
1382 798 611 363 247 232 190 179
93 90 76 74 62 21 12
BspHI T'CATG_A
Cuts at: 1101 2862 1101
Size: 1761 2669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1480 1694 1996 2377 1480
Size: 214 302 381 3533
Fragments arranged by size:
3533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 413 772 2722 2776 4260 4425 50
Size: 363 359 1950 54 1484 165 55
Fragments arranged by size:
1950 1484 363 359 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 270 376 390 518 695 919 965 2428 2609
Size: 106 14 128 177 224 46 1463 181
Cuts at: 2609 270
Size: 2091
Fragments arranged by size:
2091 1463 224 181 177 128 106 46
14
BssHII G'CGCG_C
Cuts at: 292 294 942 292
Size: 2 648 3780
Fragments arranged by size:
3780 648 2
BssSI C'ACGA_G
Cuts at: 126 874 1287 2701 3409 126
Size: 748 413 1414 708 1147
Fragments arranged by size:
1414 1147 748 708 413
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstYI r'GATC_y
Cuts at: 1 626 644 1455 1922 1945 2280 2526 2832
Size: 625 18 811 467 23 335 246 306
Cuts at: 2832 2844 2930 3982 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 811 625 467 449 335 306 246
86 23 18 12
BtgI C'CryG_G
Cuts at: 107 455 609 622 893 947 107
Size: 348 154 13 271 54 3590
Fragments arranged by size:
3590 348 271 154 54 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 126 162 2380 2561 2724 126
Size: 36 2218 181 163 1832
Fragments arranged by size:
2218 1832 181 163 36
BtsI GCAGTG_nn'
Cuts at: 1001 1673 1001
Size: 672 3758
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 1165 2136 3480 1165
Size: 971 1344 2115
Fragments arranged by size:
2115 1344 971
EaeI y'GGCC_r
Cuts at: 115 405 520 595 697 718 785 2015 2189
Size: 290 115 75 102 21 67 1230 174
Cuts at: 2189 2580 2607 3952 115
Size: 391 27 1345 593
Fragments arranged by size:
1345 1230 593 391 290 174 115 102
75 67 27 21
EagI C'GGCC_G
Cuts at: 405 520 697 785 2015 405
Size: 115 177 88 1230 2820
Fragments arranged by size:
2820 1230 177 115 88
EarI CTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 782 820 967 2638 3380 3526 4240 782
Size: 38 147 1671 742 146 714 972
Fragments arranged by size:
1671 972 742 714 147 146 38
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2687 3040 3702 2255
Size: 432 353 662 2983
Fragments arranged by size:
2983 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 131 400 864 1350 1569 131
Size: 269 464 486 219 2992
Fragments arranged by size:
2992 486 464 269 219
EcoRI G'AATT_C
Cuts at: 1018 1434 1018
Size: 416 4014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4430
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 233 277 367 673 688 696 717 1864 2112
Size: 44 90 306 15 8 21 1147 248
Cuts at: 2112 3342 4113 233
Size: 1230 771 550
Fragments arranged by size:
1230 1147 771 550 306 248 90 44
21 15 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2272 2302 2304 2334 2272
Size: 30 2 30 4368
Fragments arranged by size:
4368 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 229 669 684 1860 2108 229
Size: 440 15 1176 248 2551
Fragments arranged by size:
2551 1176 440 248 15
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 314 569 3189 3373 143
Size: 171 255 2620 184 1200
Fragments arranged by size:
2620 1200 255 184 171
MscI TGG'CCA
Cuts at: 720 2191 720
Size: 1471 2959
MslI CAynn'nnrTG
Cuts at: 181 2419 2546 181
Size: 2238 127 2065
Fragments arranged by size:
2238 2065 127
MspA1I CmG'CkG
Cuts at: 189 457 501 515 600 611 736 888 895
Size: 268 44 14 85 11 125 152 7
Cuts at: 895 949 1156 1541 1855 2215 2999 3244 189
Size: 54 207 385 314 360 784 245 1375
Fragments arranged by size:
1375 784 385 360 314 268 245 207
152 125 85 54 44 14 11 7
NaeI GCC'GGC
Cuts at: 272 392 520 697 2611 272
Size: 120 128 177 1914 2091
Fragments arranged by size:
2091 1914 177 128 120
NarI GG'CG_CC
Cuts at: 230 670 685 1861 2109 230
Size: 440 15 1176 248 2551
Fragments arranged by size:
2551 1176 440 248 15
NcoI C'CATG_G
Cuts at: 107 107
Size: 4430
NgoMIV G'CCGG_C
Cuts at: 270 390 518 695 2609 270
Size: 120 128 177 1914 2091
Fragments arranged by size:
2091 1914 177 128 120
NspI r_CATG'y
Cuts at: 1483 1983 3586 1483
Size: 500 1603 2327
Fragments arranged by size:
2327 1603 500
PasI CC'CwG_GG
Cuts at: 324 324
Size: 4430
PciI A'CATG_T
Cuts at: 3582 3582
Size: 4430
PflMI CCAn_nnn'nTGG
Cuts at: 113 367 113
Size: 254 4176
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 4398
PspOMI G'GGCC_C
Cuts at: 399 420 1350 399
Size: 21 930 3479
Fragments arranged by size:
3479 930 21
PstI C_TGCA'G
Cuts at: 1477 2162 1477
Size: 685 3745
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 515 1156 1855 2215 515
Size: 641 699 360 2730
Fragments arranged by size:
2730 699 641 360
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SacII CC_GC'GG
Cuts at: 458 612 896 950 458
Size: 154 284 54 3938
Fragments arranged by size:
3938 284 154 54
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 205 3947 205
Size: 3742 688
SfcI C'TryA_G
Cuts at: 33 1008 1113 1197 1473 2158 3126 3317 33
Size: 975 105 84 276 685 968 191 1146
Fragments arranged by size:
1146 975 968 685 276 191 105 84
SfoI GGC'GCC
Cuts at: 231 671 686 1862 2110 231
Size: 440 15 1176 248 2551
Fragments arranged by size:
2551 1176 440 248 15
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 227 349 425 745 1452 227
Size: 122 76 320 707 3205
Fragments arranged by size:
3205 707 320 122 76
SmlI C'TyrA_G
Cuts at: 1585 2500 3214 3476 1585
Size: 915 714 262 2539
Fragments arranged by size:
2539 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
SrfI GCCC'GGGC
Cuts at: 349 425 349
Size: 76 4354
StyI C'CwwG_G
Cuts at: 107 1564 1918 107
Size: 1457 354 2619
Fragments arranged by size:
2619 1457 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2013 2146 2605 2814 2013
Size: 133 459 209 3629
Fragments arranged by size:
3629 459 209 133
TatI w'GTAC_w
Cuts at: 203 1073 3945 203
Size: 870 2872 688
Fragments arranged by size:
2872 870 688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 3947 3979 607
Size: 32 3308 32 1058
Fragments arranged by size:
3308 1058 32 32
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4430
XmaI C'CCGG_G
Cuts at: 225 347 423 743 1450 225
Size: 122 76 320 707 3205
Fragments arranged by size:
3205 707 320 122 76
XmnI GAAnn'nnTTC
Cuts at: 583 1018 583
Size: 435 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI AvrII
BaeI BbsI BfrBI BmgBI BmtI BpmI BsiWI BsmI
BspEI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI ZraI