Restriction map for
pF1KA1680
(Circular) (Six-base) MAPSORT of: pF1KA1680.seq Check: 2628 from: 1 to: 5813
pF1KA1680 5813 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2851 2851
Size: 5813
Acc65I G'GTAC_C
Cuts at: 2829 2829
Size: 5813
AclI AA'CG_TT
Cuts at: 5653 5653
Size: 5813
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 920 2117 2173 2509 2534 2617 3638 4070
Size: 57 1197 56 336 25 83 1021 432
Cuts at: 4070 4423 5085 863
Size: 353 662 1591
Fragments arranged by size:
1591 1197 1021 662 432 353 336 83
57 56 25
AflIII A'CryG_T
Cuts at: 1323 2729 3165 4965 5246 1323
Size: 1406 436 1800 281 1890
Fragments arranged by size:
1890 1800 1406 436 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2797 2797
Size: 5813
AlwNI CAG_nnn'CTG
Cuts at: 414 3118 4556 414
Size: 2704 1438 1671
Fragments arranged by size:
2704 1671 1438
ApaLI G'TGCA_C
Cuts at: 4651 4651
Size: 5813
ApoI r'AATT_y
Cuts at: 676 730 883 1678 1810 2817 5166 5422 676
Size: 54 153 795 132 1007 2349 256 1067
Fragments arranged by size:
2349 1067 1007 795 256 153 132 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 5813
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5813
AvaI C'yCGr_G
Cuts at: 2833 2833
Size: 5813
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 709 742 709
Size: 33 5780
BamHI G'GATC_C
Cuts at: 1334 2838 1334
Size: 1504 4309
BanI G'GyrC_C
Cuts at: 2829 3243 3491 3526 2829
Size: 414 248 35 5116
Fragments arranged by size:
5116 414 248 35
BanII G_rGCy'C
Cuts at: 2593 2624 2827 3857 2593
Size: 31 203 1030 4549
Fragments arranged by size:
4549 1030 203 31
BbeI G_GCGC'C
Cuts at: 3247 3495 3247
Size: 248 5565
BbsI GAAGACnn'nnnn_
Cuts at: 186 453 611 1690 186
Size: 267 158 1079 4309
Fragments arranged by size:
4309 1079 267 158
BciVI GTATCCnnnnn_n'
Cuts at: 3711 4767 5813 3711
Size: 1056 1046 3711
Fragments arranged by size:
3711 1056 1046
BclI T'GATC_A
Cuts at: 1377 1863 3333 1377
Size: 486 1470 3857
Fragments arranged by size:
3857 1470 486
BfrBI ATG'CAT
Cuts at: 925 925
Size: 5813
BglII A'GATC_T
Cuts at: 493 3328 5365 493
Size: 2835 2037 941
Fragments arranged by size:
2835 2037 941
BlpI GC'TnA_GC
Cuts at: 2925 2925
Size: 5813
Bme1580I G_kGCm'C
Cuts at: 3438 3531 4655 3438
Size: 93 1124 4596
Fragments arranged by size:
4596 1124 93
BmgBI CAC'GTC
Cuts at: 2072 2072
Size: 5813
BmrI ACTGGGnnnn_n'
Cuts at: 3025 3106 3441 5585 3025
Size: 81 335 2144 3253
Fragments arranged by size:
3253 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4180 4180
Size: 5813
Bpu10I CC'TnA_GC
Cuts at: 264 341 1817 264
Size: 77 1476 4260
Fragments arranged by size:
4260 1476 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 2131 2989 3868 4582 4880 243
Size: 1888 858 879 714 298 1176
Fragments arranged by size:
1888 1176 879 858 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 147 5517 147
Size: 5370 443
BsaAI yAC'GTr
Cuts at: 3166 3166
Size: 5813
BsaBI GATnn'nnATC
Cuts at: 6 3327 5364 6
Size: 3321 2037 455
Fragments arranged by size:
3321 2037 455
BsaHI Gr'CG_yC
Cuts at: 3244 3492 4194 5690 3244
Size: 248 702 1496 3367
Fragments arranged by size:
3367 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1119 3002 3523 4201 4612 4759 5033 1119
Size: 1883 521 678 411 147 274 1899
Fragments arranged by size:
1899 1883 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 300 1494 300
Size: 1194 4619
BseYI C'CCAG_C
Cuts at: 1263 2199 3238 3996 4661 5069 1263
Size: 936 1039 758 665 408 2007
Fragments arranged by size:
2007 1039 936 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1974 2063 1974
Size: 89 5724
BsiEI CG_ry'CG
Cuts at: 104 3355 3401 4631 104
Size: 3251 46 1230 1286
Fragments arranged by size:
3251 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 387 530 2593 2827 3605 3795 4655 387
Size: 143 2063 234 778 190 860 1545
Fragments arranged by size:
2063 1545 860 778 234 190 143
BsmI GAATG_Cn'
Cuts at: 1901 2140 2673 1901
Size: 239 533 5041
Fragments arranged by size:
5041 533 239
BsmBI CGTCTCn'nnnn_
Cuts at: 1746 1746
Size: 5813
Bsp1286I G_dGCh'C
Cuts at: 387 530 2593 2624 2827 3438 3531 3605 3795
Size: 143 2063 31 203 611 93 74 190
Cuts at: 3795 3857 4655 387
Size: 62 798 1545
Fragments arranged by size:
2063 1545 798 611 203 190 143 93
74 62 31
BspHI T'CATG_A
Cuts at: 4245 4245
Size: 5813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1182 1576 2681 2863 3077 3379 3760 1182
Size: 394 1105 182 214 302 381 3235
Fragments arranged by size:
3235 1105 394 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 4105 4159 5643 5808 50
Size: 4055 54 1484 165 55
Fragments arranged by size:
4055 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3725 3725
Size: 5813
BsrFI r'CCGG_y
Cuts at: 3811 3992 3811
Size: 181 5632
BsrGI T'GTAC_A
Cuts at: 2732 2732
Size: 5813
BssSI C'ACGA_G
Cuts at: 4084 4792 4084
Size: 708 5105
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 3190 1423
Size: 1767 4046
BstBI TT'CG_AA
Cuts at: 4174 4174
Size: 5813
BstXI CCAn_nnnn'nTGG
Cuts at: 714 2803 714
Size: 2089 3724
BstYI r'GATC_y
Cuts at: 1 493 1334 1636 2838 3305 3328 3663 3909
Size: 492 841 302 1202 467 23 335 246
Cuts at: 3909 4215 4227 4313 5365 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1202 1052 841 492 467 449 335 306
302 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5813
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3763 3944 4107 3763
Size: 181 163 5469
Fragments arranged by size:
5469 181 163
BtsI GCAGTG_nn'
Cuts at: 1505 3056 1505
Size: 1551 4262
DraI TTT'AAA
Cuts at: 2812 2812
Size: 5813
DrdI GACnn_nn'nnGTC
Cuts at: 3519 4863 3519
Size: 1344 4469
EaeI y'GGCC_r
Cuts at: 969 3398 3572 3963 3990 5335 969
Size: 2429 174 391 27 1345 1447
Fragments arranged by size:
2429 1447 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3398 3398
Size: 5813
EarI CTCTTCn'nnn_
Cuts at: 197 2017 2093 2189 3836 4046 197
Size: 1820 76 96 1647 210 1964
Fragments arranged by size:
1964 1820 1647 210 96 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4021 4763 4909 5623 4021
Size: 742 146 714 4211
Fragments arranged by size:
4211 742 714 146
EcoICRI GAG'CTC
Cuts at: 2591 2825 2591
Size: 234 5579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 920 2117 2173 2509 2534 2617 3638 4070
Size: 57 1197 56 336 25 83 1021 432
Cuts at: 4070 4423 5085 863
Size: 353 662 1591
Fragments arranged by size:
1591 1197 1021 662 432 353 336 83
57 56 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1928 1928
Size: 5813
EcoO109I rG'GnC_Cy
Cuts at: 2952 2952
Size: 5813
EcoRI G'AATT_C
Cuts at: 730 2817 730
Size: 2087 3726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 2549 3832 564
Size: 1985 1283 2545
Fragments arranged by size:
2545 1985 1283
FspI TGC'GCA
Cuts at: 3594 3594
Size: 5813
HaeII r_GCGC'y
Cuts at: 3247 3495 4725 5496 3247
Size: 248 1230 771 3564
Fragments arranged by size:
3564 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 895 927 970 1002 2122 2154 2340
Size: 32 757 32 43 32 1120 32 186
Cuts at: 2340 2372 2702 2734 3655 3685 3687 3717 106
Size: 32 330 32 921 30 2 30 2202
Fragments arranged by size:
2202 1120 921 757 330 186 43 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 635 2458 2852 635
Size: 1823 394 3596
Fragments arranged by size:
3596 1823 394
KasI G'GCGC_C
Cuts at: 3243 3491 3243
Size: 248 5565
KpnI G_GTAC'C
Cuts at: 2833 2833
Size: 5813
MluI A'CGCG_T
Cuts at: 5246 5246
Size: 5813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4572 4756 4572
Size: 184 5629
MscI TGG'CCA
Cuts at: 971 3574 971
Size: 2603 3210
MslI CAynn'nnrTG
Cuts at: 1409 3802 3929 1409
Size: 2393 127 3293
Fragments arranged by size:
3293 2393 127
MspA1I CmG'CkG
Cuts at: 2157 2422 2924 3238 3598 4382 4627 2157
Size: 265 502 314 360 784 245 3343
Fragments arranged by size:
3343 784 502 360 314 265 245
NaeI GCC'GGC
Cuts at: 3994 3994
Size: 5813
NarI GG'CG_CC
Cuts at: 3244 3492 3244
Size: 248 5565
NcoI C'CATG_G
Cuts at: 107 107
Size: 5813
NgoMIV G'CCGG_C
Cuts at: 3992 3992
Size: 5813
NsiI A_TGCA'T
Cuts at: 927 927
Size: 5813
NspI r_CATG'y
Cuts at: 1327 2733 2866 3366 4969 1327
Size: 1406 133 500 1603 2171
Fragments arranged by size:
2171 1603 1406 500 133
PciI A'CATG_T
Cuts at: 1323 2729 4965 1323
Size: 1406 2236 2171
Fragments arranged by size:
2236 2171 1406
PmeI GTTT'AAAC
Cuts at: 2812 2812
Size: 5813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 5781
PshAI GACnn'nnGTC
Cuts at: 2051 2051
Size: 5813
PsiI TTA'TAA
Cuts at: 553 553
Size: 5813
PstI C_TGCA'G
Cuts at: 1512 2156 2860 3545 1512
Size: 644 704 685 3780
Fragments arranged by size:
3780 704 685 644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5813
PvuII CAG'CTG
Cuts at: 2157 3238 3598 2157
Size: 1081 360 4372
Fragments arranged by size:
4372 1081 360
RsrII CG'GwC_CG
Cuts at: 4008 4008
Size: 5813
SacI G_AGCT'C
Cuts at: 2593 2827 2593
Size: 234 5579
SalI G'TCGA_C
Cuts at: 2850 2850
Size: 5813
SapI GCTCTTCn'nnn_
Cuts at: 2093 2189 3836 4046 2093
Size: 96 1647 210 3860
Fragments arranged by size:
3860 1647 210 96
SbfI CC_TGCA'GG
Cuts at: 2860 2860
Size: 5813
ScaI AGT'ACT
Cuts at: 5330 5330
Size: 5813
SexAI A'CCwGG_T
Cuts at: 390 390
Size: 5813
SfcI C'TryA_G
Cuts at: 33 694 1508 2152 2856 3541 4509 4700 33
Size: 661 814 644 704 685 968 191 1146
Fragments arranged by size:
1146 968 814 704 685 661 644 191
SfoI GGC'GCC
Cuts at: 3245 3493 3245
Size: 248 5565
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5813
SmaI CCC'GGG
Cuts at: 2835 2835
Size: 5813
SmlI C'TyrA_G
Cuts at: 258 2146 2968 3883 4597 4859 258
Size: 1888 822 915 714 262 1212
Fragments arranged by size:
1888 1212 915 822 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5813
SphI G_CATG'C
Cuts at: 2866 3366 2866
Size: 500 5313
SspI AAT'ATT
Cuts at: 158 158
Size: 5813
StyI C'CwwG_G
Cuts at: 107 1279 2947 3301 107
Size: 1172 1668 354 2619
Fragments arranged by size:
2619 1668 1172 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2721 3396 3529 3988 4197 2721
Size: 675 133 459 209 4337
Fragments arranged by size:
4337 675 459 209 133
TatI w'GTAC_w
Cuts at: 2307 2732 5328 2307
Size: 425 2596 2792
Fragments arranged by size:
2792 2596 425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 5330 5362 2061
Size: 32 3237 32 2512
Fragments arranged by size:
3237 2512 32 32
Tth111I GACn'n_nGTC
Cuts at: 3610 3610
Size: 5813
XbaI T'CTAG_A
Cuts at: 2844 2844
Size: 5813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 3308 1916
Size: 1392 4421
XmaI C'CCGG_G
Cuts at: 2833 2833
Size: 5813
XmnI GAAnn'nnTTC
Cuts at: 450 450
Size: 5813
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BbvCI BcgI BglI BmtI BpmI
BsaXI BsiWI BspEI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI