Restriction map for
pF1KA1531
(Circular) (Six-base) MAPSORT of: pF1KA1531.seq Check: 4050 from: 1 to: 6476
pF1KA1531 6476 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 707 1616 707
Size: 909 5567
AatII G_ACGT'C
Cuts at: 3398 3398
Size: 6476
AccI GT'mk_AC
Cuts at: 1538 1775 3514 1538
Size: 237 1739 4500
Fragments arranged by size:
4500 1739 237
Acc65I G'GTAC_C
Cuts at: 950 3492 950
Size: 2542 3934
AclI AA'CG_TT
Cuts at: 6316 6316
Size: 6476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1185 4301 4733 5480 5748 529
Size: 656 3116 432 747 268 1257
Fragments arranged by size:
3116 1257 747 656 432 268
AfeI AGC'GCT
Cuts at: 2584 2584
Size: 6476
AflII C'TTAA_G
Cuts at: 652 652
Size: 6476
AflIII A'CryG_T
Cuts at: 3828 4932 5909 3828
Size: 1104 977 4395
Fragments arranged by size:
4395 1104 977
AgeI A'CCGG_T
Cuts at: 4864 4864
Size: 6476
AleI CACnn'nnGTG
Cuts at: 1267 1381 1396 2663 1267
Size: 114 15 1267 5080
Fragments arranged by size:
5080 1267 114 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1606 1807 1996 1606
Size: 201 189 6086
Fragments arranged by size:
6086 201 189
AlwNI CAG_nnn'CTG
Cuts at: 792 3781 5348 792
Size: 2989 1567 1920
Fragments arranged by size:
2989 1920 1567
ApaI G_GGCC'C
Cuts at: 723 834 2471 2751 2798 2854 723
Size: 111 1637 280 47 56 4345
Fragments arranged by size:
4345 1637 280 111 56 47
ApaLI G'TGCA_C
Cuts at: 1089 2983 5246 1089
Size: 1894 2263 2319
Fragments arranged by size:
2319 2263 1894
ApoI r'AATT_y
Cuts at: 1522 3480 5829 6085 1522
Size: 1958 2349 256 1913
Fragments arranged by size:
2349 1958 1913 256
AscI GG'CGCG_CC
Cuts at: 206 2697 206
Size: 2491 3985
AseI AT'TA_AT
Cuts at: 20 20
Size: 6476
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6476
AvaI C'yCGr_G
Cuts at: 193 200 246 337 1290 1298 2464 2594 2744
Size: 7 46 91 953 8 1166 130 150
Cuts at: 2744 2948 3168 3496 193
Size: 204 220 328 3173
Fragments arranged by size:
3173 1166 953 328 220 204 150 130
91 46 8 7
BamHI G'GATC_C
Cuts at: 3501 3501
Size: 6476
BanI G'GyrC_C
Cuts at: 112 138 189 361 484 670 950 1285 1294
Size: 26 51 172 123 186 280 335 9
Cuts at: 1294 1657 1747 2058 2233 2944 3172 3367 3492
Size: 363 90 311 175 711 228 195 125
Cuts at: 3492 3906 4154 4189 112
Size: 414 248 35 2399
Fragments arranged by size:
2399 711 414 363 335 311 280 248
228 195 186 175 172 125 123 90
51 35 26 9
BanII G_rGCy'C
Cuts at: 344 404 723 834 966 2048 2471 2568 2751
Size: 60 319 111 132 1082 423 97 183
Cuts at: 2751 2798 2854 3200 3490 4520 344
Size: 47 56 346 290 1030 2300
Fragments arranged by size:
2300 1082 1030 423 346 319 290 183
132 111 97 60 56 47
BbeI G_GCGC'C
Cuts at: 116 142 193 488 1751 2237 3176 3371 3910
Size: 26 51 295 1263 486 939 195 539
Cuts at: 3910 4158 116
Size: 248 2434
Fragments arranged by size:
2434 1263 939 539 486 295 248 195
51 26
BbsI GAAGACnn'nnnn_
Cuts at: 2643 3445 2643
Size: 802 5674
BbvCI CC'TCA_GC
Cuts at: 1687 2374 1687
Size: 687 5789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1162 1196 1632 1666 1162
Size: 34 436 34 5972
Fragments arranged by size:
5972 436 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1265 4374 5135 6476 1265
Size: 3109 761 1341 1265
Fragments arranged by size:
3109 1341 1265 761
BclI T'GATC_A
Cuts at: 3996 3996
Size: 6476
BglI GCCn_nnn'nGGC
Cuts at: 264 2554 2563 3263 3365 264
Size: 2290 9 700 102 3375
Fragments arranged by size:
3375 2290 700 102 9
BglII A'GATC_T
Cuts at: 519 3991 6028 519
Size: 3472 2037 967
Fragments arranged by size:
3472 2037 967
BlpI GC'TnA_GC
Cuts at: 268 1720 3588 268
Size: 1452 1868 3156
Fragments arranged by size:
3156 1868 1452
Bme1580I G_kGCm'C
Cuts at: 673 723 777 834 1093 1274 1290 1297 1662
Size: 50 54 57 259 181 16 7 365
Cuts at: 1662 2061 2471 2751 2798 2854 2947 2987 4101
Size: 399 410 280 47 56 93 40 1114
Cuts at: 4101 4194 5250 673
Size: 93 1056 1899
Fragments arranged by size:
1899 1114 1056 410 399 365 280 259
181 93 93 57 56 54 50 47
40 16 7
BmgBI CAC'GTC
Cuts at: 2682 2682
Size: 6476
BmrI ACTGGGnnnn_n'
Cuts at: 764 2093 2450 3688 3769 4104 6248 764
Size: 1329 357 1238 81 335 2144 992
Fragments arranged by size:
2144 1329 1238 992 357 335 81
BmtI G_CTAG'C
Cuts at: 1475 1475
Size: 6476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4843 4843
Size: 6476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 597 787 856 1165 1684 2073 2150 2377
Size: 140 190 69 309 519 389 77 227
Cuts at: 2377 3112 3334 457
Size: 735 222 3599
Fragments arranged by size:
3599 735 519 389 309 227 222 190
140 77 69
Bpu10I CC'TnA_GC
Cuts at: 785 1687 2374 2392 785
Size: 902 687 18 4869
Fragments arranged by size:
4869 902 687 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 2791 3043 3280 3652 4531 5023 5321 399
Size: 2392 252 237 372 879 492 298 1554
Fragments arranged by size:
2392 1554 879 492 372 298 252 237
BsaI GGTCTCn'nnnn_
Cuts at: 550 1141 6180 550
Size: 591 5039 846
Fragments arranged by size:
5039 846 591
BsaAI yAC'GTr
Cuts at: 3829 3829
Size: 6476
BsaBI GATnn'nnATC
Cuts at: 6 1408 3990 6027 6
Size: 1402 2582 2037 455
Fragments arranged by size:
2582 2037 1402 455
BsaHI Gr'CG_yC
Cuts at: 113 139 190 278 485 1748 2220 2234 2461
Size: 26 51 88 207 1263 472 14 227
Cuts at: 2461 2994 3173 3368 3395 3907 4155 4857 6353
Size: 533 179 195 27 512 248 702 1496
Cuts at: 6353 113
Size: 236
Fragments arranged by size:
1496 1263 702 533 512 472 248 236
227 207 195 179 88 51 27 26
14
BsaWI w'CCGG_w
Cuts at: 533 2111 3442 3665 4186 4864 5138 5285 5696
Size: 1578 1331 223 521 678 274 147 411
Cuts at: 5696 533
Size: 1313
Fragments arranged by size:
1578 1331 1313 678 521 411 274 223
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 1828 1858 1975 2005 3085 3115 595
Size: 30 1203 30 117 30 1080 30 3956
Fragments arranged by size:
3956 1203 1080 117 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1980 2383 1980
Size: 403 6073
BseYI C'CCAG_C
Cuts at: 812 1331 1622 1700 2579 3901 4659 5236 5732
Size: 519 291 78 879 1322 758 577 496
Cuts at: 5732 812
Size: 1556
Fragments arranged by size:
1556 1322 879 758 577 519 496 291
78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 496 826 1008 1747 1767 1966 2625 3106
Size: 166 330 182 739 20 199 659 481
Cuts at: 3106 330
Size: 3700
Fragments arranged by size:
3700 739 659 481 330 199 182 166
20
BsiEI CG_ry'CG
Cuts at: 104 1329 2915 4018 4064 5272 104
Size: 1225 1586 1103 46 1208 1308
Fragments arranged by size:
1586 1308 1225 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 247 913 1093 1607 2036 2048 2987 3490 4268
Size: 666 180 514 429 12 939 503 778
Cuts at: 4268 4458 5250 247
Size: 190 792 1473
Fragments arranged by size:
1473 939 792 778 666 514 503 429
190 180 12
Bsp1286I G_dGCh'C
Cuts at: 247 344 404 673 723 777 834 913 966
Size: 97 60 269 50 54 57 79 53
Cuts at: 966 1093 1274 1290 1297 1607 1662 2036 2048
Size: 127 181 16 7 310 55 374 12
Cuts at: 2048 2061 2471 2568 2751 2798 2854 2947 2987
Size: 13 410 97 183 47 56 93 40
Cuts at: 2987 3200 3490 4101 4194 4268 4458 4520 5250
Size: 213 290 611 93 74 190 62 730
Cuts at: 5250 247
Size: 1473
Fragments arranged by size:
1473 730 611 410 374 310 290 269
213 190 183 181 127 97 97 93
93 79 74 62 60 57 56 55
54 53 50 47 40 16 13 12
7
BspEI T'CCGG_A
Cuts at: 5696 5696
Size: 6476
BspHI T'CATG_A
Cuts at: 5652 5652
Size: 6476
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 1616 2879 3526 3740 4042 4423 707
Size: 909 1263 647 214 302 381 2760
Fragments arranged by size:
2760 1263 909 647 381 302 214
BsrBI CCG'CTC
Cuts at: 50 255 272 2086 3113 3335 3345 4768 4822
Size: 205 17 1814 1027 222 10 1423 54
Cuts at: 4822 6306 6471 50
Size: 1484 165 55
Fragments arranged by size:
1814 1484 1423 1027 222 205 165 55
54 17 10
BsrDI GCAATG_nn'
Cuts at: 414 4388 414
Size: 3974 2502
BsrFI r'CCGG_y
Cuts at: 1310 1324 2914 2937 3053 3175 4474 4655 4864
Size: 14 1590 23 116 122 1299 181 209
Cuts at: 4864 1310
Size: 2922
Fragments arranged by size:
2922 1590 1299 209 181 122 116 23
14
BsrGI T'GTAC_A
Cuts at: 1388 1454 1388
Size: 66 6410
BssHII G'CGCG_C
Cuts at: 145 206 2447 2697 3308 145
Size: 61 2241 250 611 3313
Fragments arranged by size:
3313 2241 611 250 61
BssSI C'ACGA_G
Cuts at: 2691 4747 5105 2691
Size: 2056 358 4062
Fragments arranged by size:
4062 2056 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1878 3853 1878
Size: 1975 4501
BstBI TT'CG_AA
Cuts at: 4837 4837
Size: 6476
BstEII G'GTnAC_C
Cuts at: 2523 2523
Size: 6476
BstXI CCAn_nnnn'nTGG
Cuts at: 924 1740 924
Size: 816 5660
BstYI r'GATC_y
Cuts at: 1 519 2276 3501 3968 3991 4326 4572 5584
Size: 518 1757 1225 467 23 335 246 1012
Cuts at: 5584 5670 5682 6028 1
Size: 86 12 346 449
Fragments arranged by size:
1757 1225 1012 518 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1776 1776
Size: 6476
Bsu36I CC'TnA_GG
Cuts at: 451 1074 451
Size: 623 5853
BtgI C'CryG_G
Cuts at: 107 169 1100 1191 1769 2723 2988 107
Size: 62 931 91 578 954 265 3595
Fragments arranged by size:
3595 954 931 578 265 91 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3313 4426 4607 4770 3313
Size: 1113 181 163 5019
Fragments arranged by size:
5019 1113 181 163
BtsI GCAGTG_nn'
Cuts at: 430 2579 3719 430
Size: 2149 1140 3187
Fragments arranged by size:
3187 2149 1140
DraI TTT'AAA
Cuts at: 3475 3475
Size: 6476
DraIII CAC_nnn'GTG
Cuts at: 1770 1770
Size: 6476
DrdI GACnn_nn'nnGTC
Cuts at: 4182 5040 4182
Size: 858 5618
EaeI y'GGCC_r
Cuts at: 1001 1326 1577 2546 2832 2912 4061 4235 4626
Size: 325 251 969 286 80 1149 174 391
Cuts at: 4626 4653 5998 1001
Size: 27 1345 1479
Fragments arranged by size:
1479 1345 1149 969 391 325 286 251
174 80 27
EagI C'GGCC_G
Cuts at: 1326 2912 4061 1326
Size: 1586 1149 3741
Fragments arranged by size:
3741 1586 1149
EarI CTCTTCn'nnn_
Cuts at: 1813 2650 3448 4499 4709 1813
Size: 837 798 1051 210 3580
Fragments arranged by size:
3580 1051 837 798 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 312 2622 2688 4684 4994 5140 6286 312
Size: 2310 66 1996 310 146 1146 502
Fragments arranged by size:
2310 1996 1146 502 310 146 66
EcoICRI GAG'CTC
Cuts at: 2046 3488 2046
Size: 1442 5034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 529 597 787 856 1165 1185 1684 2073
Size: 72 68 190 69 309 20 499 389
Cuts at: 2073 2150 2377 3112 3334 4301 4733 5480 5748
Size: 77 227 735 222 967 432 747 268
Cuts at: 5748 457
Size: 1185
Fragments arranged by size:
1185 967 747 735 499 432 389 309
268 227 222 190 77 72 69 68
20
EcoNI CCTnn'n_nnAGG
Cuts at: 805 1731 805
Size: 926 5550
EcoO109I rG'GnC_Cy
Cuts at: 321 567 789 830 831 2079 2311 2348 2468
Size: 246 222 41 1 1248 232 37 120
Cuts at: 2468 2748 2794 2795 2851 3063 3615 321
Size: 280 46 1 56 212 552 3182
Fragments arranged by size:
3182 1248 552 280 246 232 222 212
120 56 46 41 37 1 1
EcoRI G'AATT_C
Cuts at: 3480 3480
Size: 6476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4495 4495
Size: 6476
FspI TGC'GCA
Cuts at: 4257 4257
Size: 6476
HaeII r_GCGC'y
Cuts at: 116 142 193 488 1727 1751 2237 2521 2586
Size: 26 51 295 1239 24 486 284 65
Cuts at: 2586 3091 3176 3236 3273 3281 3371 3910 4158
Size: 505 85 60 37 8 90 539 248
Cuts at: 4158 5180 6159 116
Size: 1022 979 433
Fragments arranged by size:
1239 1022 979 539 505 486 433 295
284 248 90 85 65 60 51 37
26 24 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 528 560 1038 1070 1422 1454 3085 3102 3117
Size: 32 478 32 352 32 1631 17 15
Cuts at: 3117 3134 4318 4348 4350 4380 528
Size: 17 1184 30 2 30 2624
Fragments arranged by size:
2624 1631 1184 478 352 32 32 32
30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 1539 2172 3515 1539
Size: 633 1343 4500
Fragments arranged by size:
4500 1343 633
KasI G'GCGC_C
Cuts at: 112 138 189 484 1747 2233 3172 3367 3906
Size: 26 51 295 1263 486 939 195 539
Cuts at: 3906 4154 112
Size: 248 2434
Fragments arranged by size:
2434 1263 939 539 486 295 248 195
51 26
KpnI G_GTAC'C
Cuts at: 954 3496 954
Size: 2542 3934
MluI A'CGCG_T
Cuts at: 5909 5909
Size: 6476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 551 609 725 5147 5331 551
Size: 58 116 4422 184 1696
Fragments arranged by size:
4422 1696 184 116 58
MscI TGG'CCA
Cuts at: 1579 2548 4237 1579
Size: 969 1689 3818
Fragments arranged by size:
3818 1689 969
MslI CAynn'nnrTG
Cuts at: 1063 1267 1381 1396 1711 1852 1903 2248 2663
Size: 204 114 15 315 141 51 345 415
Cuts at: 2663 3403 4465 4592 1063
Size: 740 1062 127 2947
Fragments arranged by size:
2947 1062 740 415 345 315 204 141
127 114 51 15
MspA1I CmG'CkG
Cuts at: 162 236 314 741 947 1193 1331 1446 2164
Size: 74 78 427 206 246 138 115 718
Cuts at: 2164 2402 2609 2725 2831 2841 2877 3186 3425
Size: 238 207 116 106 10 36 309 239
Cuts at: 3425 3587 3901 4261 5274 5519 162
Size: 162 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 718 427 360 314 309 246
245 239 238 207 206 162 138 116
115 106 78 74 36 10
NaeI GCC'GGC
Cuts at: 1326 2916 2939 3177 4657 1326
Size: 1590 23 238 1480 3145
Fragments arranged by size:
3145 1590 1480 238 23
NarI GG'CG_CC
Cuts at: 113 139 190 485 1748 2234 3173 3368 3907
Size: 26 51 295 1263 486 939 195 539
Cuts at: 3907 4155 113
Size: 248 2434
Fragments arranged by size:
2434 1263 939 539 486 295 248 195
51 26
NcoI C'CATG_G
Cuts at: 107 1100 107
Size: 993 5483
NgoMIV G'CCGG_C
Cuts at: 1324 2914 2937 3175 4655 1324
Size: 1590 23 238 1480 3145
Fragments arranged by size:
3145 1590 1480 238 23
NheI G'CTAG_C
Cuts at: 1471 1471
Size: 6476
NspI r_CATG'y
Cuts at: 784 1878 3529 4029 4936 784
Size: 1094 1651 500 907 2324
Fragments arranged by size:
2324 1651 1094 907 500
PasI CC'CwG_GG
Cuts at: 716 792 1275 1733 2637 716
Size: 76 483 458 904 4555
Fragments arranged by size:
4555 904 483 458 76
PciI A'CATG_T
Cuts at: 4932 4932
Size: 6476
PflMI CCAn_nnn'nTGG
Cuts at: 1968 2230 2841 1968
Size: 262 611 5603
Fragments arranged by size:
5603 611 262
PfoI T'CCnGG_A
Cuts at: 326 803 1858 2499 326
Size: 477 1055 641 4303
Fragments arranged by size:
4303 1055 641 477
PmeI GTTT'AAAC
Cuts at: 3475 3475
Size: 6476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 740 772 5615 5647 740
Size: 32 4843 32 1569
Fragments arranged by size:
4843 1569 32 32
PpuMI rG'GwC_Cy
Cuts at: 321 2079 2311 321
Size: 1758 232 4486
Fragments arranged by size:
4486 1758 232
PspOMI G'GGCC_C
Cuts at: 719 830 2467 2747 2794 2850 719
Size: 111 1637 280 47 56 4345
Fragments arranged by size:
4345 1637 280 111 56 47
PstI C_TGCA'G
Cuts at: 740 1253 1645 2169 2876 3185 3523 4208 740
Size: 513 392 524 707 309 338 685 3008
Fragments arranged by size:
3008 707 685 524 513 392 338 309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6476
PvuII CAG'CTG
Cuts at: 236 741 1446 2164 2609 2877 3186 3901 4261
Size: 505 705 718 445 268 309 715 360
Cuts at: 4261 236
Size: 2451
Fragments arranged by size:
2451 718 715 705 505 445 360 309
268
RsrII CG'GwC_CG
Cuts at: 4671 4671
Size: 6476
SacI G_AGCT'C
Cuts at: 2048 3490 2048
Size: 1442 5034
SacII CC_GC'GG
Cuts at: 1194 2726 1194
Size: 1532 4944
SalI G'TCGA_C
Cuts at: 1537 3513 1537
Size: 1976 4500
SanDI GG'GwC_CC
Cuts at: 2079 2079
Size: 6476
SapI GCTCTTCn'nnn_
Cuts at: 3448 4499 4709 3448
Size: 1051 210 5215
Fragments arranged by size:
5215 1051 210
SbfI CC_TGCA'GG
Cuts at: 3523 3523
Size: 6476
ScaI AGT'ACT
Cuts at: 5993 5993
Size: 6476
SfcI C'TryA_G
Cuts at: 33 736 1249 1641 2165 2872 3181 3519 4204
Size: 703 513 392 524 707 309 338 685
Cuts at: 4204 5197 5388 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 707 703 685 524 513 392
338 309 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2554 3263 2554
Size: 709 5767
SfoI GGC'GCC
Cuts at: 114 140 191 486 1749 2235 3174 3369 3908
Size: 26 51 295 1263 486 939 195 539
Cuts at: 3908 4156 114
Size: 248 2434
Fragments arranged by size:
2434 1263 939 539 486 295 248 195
51 26
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6476
SgrAI Cr'CCGG_yG
Cuts at: 1310 1310
Size: 6476
SmaI CCC'GGG
Cuts at: 1292 2466 2596 2746 2950 3170 3498 1292
Size: 1174 130 150 204 220 328 4270
Fragments arranged by size:
4270 1174 328 220 204 150 130
SmlI C'TyrA_G
Cuts at: 378 652 2806 3058 3295 3631 4546 5038 5300
Size: 274 2154 252 237 336 915 492 262
Cuts at: 5300 378
Size: 1554
Fragments arranged by size:
2154 1554 915 492 336 274 262 252
237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6476
SphI G_CATG'C
Cuts at: 784 3529 4029 784
Size: 2745 500 3231
Fragments arranged by size:
3231 2745 500
SrfI GCCC'GGGC
Cuts at: 2466 3170 2466
Size: 704 5772
StuI AGG'CCT
Cuts at: 1639 3040 1639
Size: 1401 5075
StyI C'CwwG_G
Cuts at: 107 260 1100 1107 2229 2330 3017 3374 3610
Size: 153 840 7 1122 101 687 357 236
Cuts at: 3610 3964 107
Size: 354 2619
Fragments arranged by size:
2619 1122 840 687 357 354 236 153
101 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1645 4059 4192 4651 4860 1645
Size: 2414 133 459 209 3261
Fragments arranged by size:
3261 2414 459 209 133
TatI w'GTAC_w
Cuts at: 1388 1454 2540 5991 1388
Size: 66 1086 3451 1873
Fragments arranged by size:
3451 1873 1086 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1189 1221 1975 2007 5993 6025 1189
Size: 32 754 32 3986 32 1640
Fragments arranged by size:
3986 1640 754 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1050 3394 4273 1050
Size: 2344 879 3253
Fragments arranged by size:
3253 2344 879
XbaI T'CTAG_A
Cuts at: 3507 3507
Size: 6476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1125 1950 2874 3971 1125
Size: 825 924 1097 3630
Fragments arranged by size:
3630 1097 924 825
XmaI C'CCGG_G
Cuts at: 1290 2464 2594 2744 2948 3168 3496 1290
Size: 1174 130 150 204 220 328 4270
Fragments arranged by size:
4270 1174 328 220 204 150 130
ZraI GAC'GTC
Cuts at: 3396 3396
Size: 6476
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AhdI AloI AvrII BaeI BfrBI BsiWI BsmI BsmBI
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SexAI SnaBI SspI SwaI XhoI XmnI