Restriction map for
pF1KA1497
(Circular) (Six-base) MAPSORT of: pF1KA1497.seq Check: 8263 from: 1 to: 5261
pF1KA1497 5261 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1762 1762
Size: 5261
AccI GT'mk_AC
Cuts at: 522 1016 2228 2299 522
Size: 494 1212 71 3484
Fragments arranged by size:
3484 1212 494 71
Acc65I G'GTAC_C
Cuts at: 1552 1675 2277 1552
Size: 123 602 4536
Fragments arranged by size:
4536 602 123
AclI AA'CG_TT
Cuts at: 5101 5101
Size: 5261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3086 3518 3871 4533 3086
Size: 432 353 662 3814
Fragments arranged by size:
3814 662 432 353
AflIII A'CryG_T
Cuts at: 1600 2249 2613 4413 4694 1600
Size: 649 364 1800 281 2167
Fragments arranged by size:
2167 1800 649 364 281
AleI CACnn'nnGTG
Cuts at: 331 1636 331
Size: 1305 3956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1358 1390 1358
Size: 32 5229
AlwNI CAG_nnn'CTG
Cuts at: 1471 2566 4004 1471
Size: 1095 1438 2728
Fragments arranged by size:
2728 1438 1095
ApaLI G'TGCA_C
Cuts at: 4099 4099
Size: 5261
ApoI r'AATT_y
Cuts at: 224 689 722 753 897 904 1475 2265 4614
Size: 465 33 31 144 7 571 790 2349
Cuts at: 4614 4870 224
Size: 256 615
Fragments arranged by size:
2349 790 615 571 465 256 144 33
31 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 5261
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
AvaI C'yCGr_G
Cuts at: 1634 2281 1634
Size: 647 4614
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1587 1620 1667 1700 1587
Size: 33 47 33 5148
Fragments arranged by size:
5148 47 33 33
BamHI G'GATC_C
Cuts at: 2286 2286
Size: 5261
BanI G'GyrC_C
Cuts at: 1328 1552 1675 2277 2691 2939 2974 1328
Size: 224 123 602 414 248 35 3615
Fragments arranged by size:
3615 602 414 248 224 123 35
BanII G_rGCy'C
Cuts at: 1007 1289 2275 3305 1007
Size: 282 986 1030 2963
Fragments arranged by size:
2963 1030 986 282
BbeI G_GCGC'C
Cuts at: 2695 2943 2695
Size: 248 5013
BbsI GAAGACnn'nnnn_
Cuts at: 173 370 1589 173
Size: 197 1219 3845
Fragments arranged by size:
3845 1219 197
BbvCI CC'TCA_GC
Cuts at: 532 532
Size: 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 339 373 1006 1040 339
Size: 34 633 34 4560
Fragments arranged by size:
4560 633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1219 3159 4215 5261 1219
Size: 1940 1056 1046 1219
Fragments arranged by size:
1940 1219 1056 1046
BclI T'GATC_A
Cuts at: 1980 2781 1980
Size: 801 4460
BglII A'GATC_T
Cuts at: 2776 4813 2776
Size: 2037 3224
BlpI GC'TnA_GC
Cuts at: 1460 2373 1460
Size: 913 4348
Bme1580I G_kGCm'C
Cuts at: 1331 2886 2979 4103 1331
Size: 1555 93 1124 2489
Fragments arranged by size:
2489 1555 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 302 991 1491 1825 2473 2554 2889 5033 302
Size: 689 500 334 648 81 335 2144 530
Fragments arranged by size:
2144 689 648 530 500 335 334 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 5261
Bpu10I CC'TnA_GC
Cuts at: 532 532
Size: 5261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 2437 3316 4030 4328 850
Size: 1587 879 714 298 1783
Fragments arranged by size:
1783 1587 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4965 4965
Size: 5261
BsaAI yAC'GTr
Cuts at: 2614 2614
Size: 5261
BsaBI GATnn'nnATC
Cuts at: 6 1207 2775 4812 6
Size: 1201 1568 2037 455
Fragments arranged by size:
2037 1568 1201 455
BsaHI Gr'CG_yC
Cuts at: 1759 2692 2940 3642 5138 1759
Size: 933 248 702 1496 1882
Fragments arranged by size:
1882 1496 933 702 248
BsaWI w'CCGG_w
Cuts at: 2450 2971 3649 4060 4207 4481 2450
Size: 521 678 411 147 274 3230
Fragments arranged by size:
3230 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 616 646 1276 1306 1358 1388 616
Size: 30 630 30 52 30 4489
Fragments arranged by size:
4489 630 52 30 30 30
BseYI C'CCAG_C
Cuts at: 150 464 2686 3444 4109 4517 150
Size: 314 2222 758 665 408 894
Fragments arranged by size:
2222 894 758 665 408 314
BsiEI CG_ry'CG
Cuts at: 104 1020 2803 2849 4079 104
Size: 916 1783 46 1230 1286
Fragments arranged by size:
1783 1286 1230 916 46
BsiHKAI G_wGCw'C
Cuts at: 1007 2275 3053 3243 4103 1007
Size: 1268 778 190 860 2165
Fragments arranged by size:
2165 1268 860 778 190
BsmI GAATG_Cn'
Cuts at: 1161 1161
Size: 5261
Bsp1286I G_dGCh'C
Cuts at: 1007 1289 1331 2275 2886 2979 3053 3243 3305
Size: 282 42 944 611 93 74 190 62
Cuts at: 3305 4103 1007
Size: 798 2165
Fragments arranged by size:
2165 944 798 611 282 190 93 74
62 42
BspHI T'CATG_A
Cuts at: 695 1754 3693 695
Size: 1059 1939 2263
Fragments arranged by size:
2263 1939 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2311 2525 2827 3208 2311
Size: 214 302 381 4364
Fragments arranged by size:
4364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3553 3607 5091 5256 50
Size: 3503 54 1484 165 55
Fragments arranged by size:
3503 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 288 2048 3173 288
Size: 1760 1125 2376
Fragments arranged by size:
2376 1760 1125
BsrFI r'CCGG_y
Cuts at: 3259 3440 3259
Size: 181 5080
BssSI C'ACGA_G
Cuts at: 3532 4240 3532
Size: 708 4553
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5261
BstBI TT'CG_AA
Cuts at: 3622 3622
Size: 5261
BstYI r'GATC_y
Cuts at: 1 2286 2753 2776 3111 3357 3663 3675 3761
Size: 2285 467 23 335 246 306 12 86
Cuts at: 3761 4813 1
Size: 1052 449
Fragments arranged by size:
2285 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1225 1225
Size: 5261
BtgI C'CryG_G
Cuts at: 107 1454 107
Size: 1347 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 342 3211 3392 3555 342
Size: 2869 181 163 2048
Fragments arranged by size:
2869 2048 181 163
BtsI GCAGTG_nn'
Cuts at: 1973 2504 1973
Size: 531 4730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 255 290 255
Size: 35 5226
DraI TTT'AAA
Cuts at: 2260 2260
Size: 5261
DraIII CAC_nnn'GTG
Cuts at: 1335 1335
Size: 5261
DrdI GACnn_nn'nnGTC
Cuts at: 1926 2967 4311 1926
Size: 1041 1344 2876
Fragments arranged by size:
2876 1344 1041
EaeI y'GGCC_r
Cuts at: 2846 3020 3411 3438 4783 2846
Size: 174 391 27 1345 3324
Fragments arranged by size:
3324 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5261
EarI CTCTTCn'nnn_
Cuts at: 2130 3284 3494 2130
Size: 1154 210 3897
Fragments arranged by size:
3897 1154 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3469 4211 4357 5071 3469
Size: 742 146 714 3659
Fragments arranged by size:
3659 742 714 146
EcoICRI GAG'CTC
Cuts at: 1005 2273 1005
Size: 1268 3993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 3086 3518 3871 4533 3086
Size: 432 353 662 3814
Fragments arranged by size:
3814 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1661 1827 2400 1661
Size: 166 573 4522
Fragments arranged by size:
4522 573 166
EcoRI G'AATT_C
Cuts at: 722 2265 722
Size: 1543 3718
EcoRV GAT'ATC
Cuts at: 1385 1422 1385
Size: 37 5224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 5261
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5261
HaeII r_GCGC'y
Cuts at: 2695 2943 4173 4944 2695
Size: 248 1230 771 3012
Fragments arranged by size:
3012 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 823 855 1276 1308 3103 3133 3135 3165 823
Size: 32 421 32 1795 30 2 30 2919
Fragments arranged by size:
2919 1795 421 32 32 30 30 2
HincII GTy'rAC
Cuts at: 251 1017 1656 2229 2300 251
Size: 766 639 573 71 3212
Fragments arranged by size:
3212 766 639 573 71
HindIII A'AGCT_T
Cuts at: 762 1128 762
Size: 366 4895
HpaI GTT'AAC
Cuts at: 1656 1656
Size: 5261
KasI G'GCGC_C
Cuts at: 2691 2939 2691
Size: 248 5013
KpnI G_GTAC'C
Cuts at: 1556 1679 2281 1556
Size: 123 602 4536
Fragments arranged by size:
4536 602 123
MfeI C'AATT_G
Cuts at: 142 142
Size: 5261
MluI A'CGCG_T
Cuts at: 4694 4694
Size: 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 1283 4020 4204 659
Size: 624 2737 184 1716
Fragments arranged by size:
2737 1716 624 184
MscI TGG'CCA
Cuts at: 3022 3022
Size: 5261
MslI CAynn'nnrTG
Cuts at: 331 1636 1726 2053 3250 3377 331
Size: 1305 90 327 1197 127 2215
Fragments arranged by size:
2215 1305 1197 327 127 90
MspA1I CmG'CkG
Cuts at: 2372 2686 3046 3830 4075 2372
Size: 314 360 784 245 3558
Fragments arranged by size:
3558 784 360 314 245
NaeI GCC'GGC
Cuts at: 3442 3442
Size: 5261
NarI GG'CG_CC
Cuts at: 2692 2940 2692
Size: 248 5013
NcoI C'CATG_G
Cuts at: 107 1454 107
Size: 1347 3914
NgoMIV G'CCGG_C
Cuts at: 3440 3440
Size: 5261
NruI TCG'CGA
Cuts at: 361 361
Size: 5261
NspI r_CATG'y
Cuts at: 1604 2253 2314 2814 4417 1604
Size: 649 61 500 1603 2448
Fragments arranged by size:
2448 1603 649 500 61
PciI A'CATG_T
Cuts at: 1600 2249 4413 1600
Size: 649 2164 2448
Fragments arranged by size:
2448 2164 649
PflMI CCAn_nnn'nTGG
Cuts at: 1098 1098
Size: 5261
PfoI T'CCnGG_A
Cuts at: 798 1352 1732 798
Size: 554 380 4327
Fragments arranged by size:
4327 554 380
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1358 1390 3707 3739 1358
Size: 32 2317 32 2880
Fragments arranged by size:
2880 2317 32 32
PpuMI rG'GwC_Cy
Cuts at: 1661 1827 1661
Size: 166 5095
PstI C_TGCA'G
Cuts at: 383 1868 2010 2208 2308 2993 383
Size: 1485 142 198 100 685 2651
Fragments arranged by size:
2651 1485 685 198 142 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5261
PvuII CAG'CTG
Cuts at: 2686 3046 2686
Size: 360 4901
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5261
SacI G_AGCT'C
Cuts at: 1007 2275 1007
Size: 1268 3993
SalI G'TCGA_C
Cuts at: 1015 2227 2298 1015
Size: 1212 71 3978
Fragments arranged by size:
3978 1212 71
SanDI GG'GwC_CC
Cuts at: 1661 1827 1661
Size: 166 5095
SapI GCTCTTCn'nnn_
Cuts at: 3284 3494 3284
Size: 210 5051
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5261
ScaI AGT'ACT
Cuts at: 4778 4778
Size: 5261
SexAI A'CCwGG_T
Cuts at: 1331 1331
Size: 5261
SfcI C'TryA_G
Cuts at: 33 379 1864 2006 2204 2304 2989 3957 4148
Size: 346 1485 142 198 100 685 968 191
Cuts at: 4148 33
Size: 1146
Fragments arranged by size:
1485 1146 968 685 346 198 191 142
100
SfoI GGC'GCC
Cuts at: 2693 2941 2693
Size: 248 5013
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
SmaI CCC'GGG
Cuts at: 2283 2283
Size: 5261
SmlI C'TyrA_G
Cuts at: 829 2416 3331 4045 4307 829
Size: 1587 915 714 262 1783
Fragments arranged by size:
1783 1587 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5261
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4761
SspI AAT'ATT
Cuts at: 1908 1908
Size: 5261
StyI C'CwwG_G
Cuts at: 107 1454 1556 1620 2395 2749 107
Size: 1347 102 64 775 354 2619
Fragments arranged by size:
2619 1347 775 354 102 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 693 2844 2977 3436 3645 693
Size: 2151 133 459 209 2309
Fragments arranged by size:
2309 2151 459 209 133
TatI w'GTAC_w
Cuts at: 1993 2057 4776 1993
Size: 64 2719 2478
Fragments arranged by size:
2719 2478 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 780 812 4778 4810 616
Size: 32 132 32 3966 32 1067
Fragments arranged by size:
3966 1067 132 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5261
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 2756
Size: 5261
XmaI C'CCGG_G
Cuts at: 2281 2281
Size: 5261
ZraI GAC'GTC
Cuts at: 1760 1760
Size: 5261
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BfrBI BglI BmgBI BmtI BpmI BseRI BsgI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI EcoNI FseI FspAI NdeI NheI NotI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI