Restriction map for
pF1KA1408
(Circular) (Six-base) MAPSORT of: pF1KA1408.seq Check: 805 from: 1 to: 6929
pF1KA1408 6929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1044 3967 1044
Size: 2923 4006
Acc65I G'GTAC_C
Cuts at: 3945 3945
Size: 6929
AclI AA'CG_TT
Cuts at: 6769 6769
Size: 6929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 573 814 1748 4754 5186 5539 6201 287
Size: 286 241 934 3006 432 353 662 1015
Fragments arranged by size:
3006 1015 934 662 432 353 286 241
AflII C'TTAA_G
Cuts at: 3412 3412
Size: 6929
AflIII A'CryG_T
Cuts at: 2233 2890 3921 4281 6081 6362 2233
Size: 657 1031 360 1800 281 2800
Fragments arranged by size:
2800 1800 1031 657 360 281
AhdI GACnn_n'nnGTC
Cuts at: 672 672
Size: 6929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2345 3827 2345
Size: 1482 5447
AlwNI CAG_nnn'CTG
Cuts at: 721 1332 4234 5672 721
Size: 611 2902 1438 1978
Fragments arranged by size:
2902 1978 1438 611
ApaI G_GGCC'C
Cuts at: 3569 3569
Size: 6929
ApaLI G'TGCA_C
Cuts at: 775 3511 5767 775
Size: 2736 2256 1937
Fragments arranged by size:
2736 2256 1937
ApoI r'AATT_y
Cuts at: 696 1309 1341 1503 2695 3124 3933 6282 6538
Size: 613 32 162 1192 429 809 2349 256
Cuts at: 6538 696
Size: 1087
Fragments arranged by size:
2349 1192 1087 809 613 429 256 162
32
AseI AT'TA_AT
Cuts at: 20 992 1688 20
Size: 972 696 5261
Fragments arranged by size:
5261 972 696
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
AvaI C'yCGr_G
Cuts at: 3949 3949
Size: 6929
BamHI G'GATC_C
Cuts at: 2633 3954 2633
Size: 1321 5608
BanI G'GyrC_C
Cuts at: 3945 4359 4607 4642 3945
Size: 414 248 35 6232
Fragments arranged by size:
6232 414 248 35
BanII G_rGCy'C
Cuts at: 1229 1280 3569 3943 4973 1229
Size: 51 2289 374 1030 3185
Fragments arranged by size:
3185 2289 1030 374 51
BbeI G_GCGC'C
Cuts at: 4363 4611 4363
Size: 248 6681
BbsI GAAGACnn'nnnn_
Cuts at: 275 317 275
Size: 42 6887
BbvCI CC'TCA_GC
Cuts at: 2038 3556 2038
Size: 1518 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6895
BciVI GTATCCnnnnn_n'
Cuts at: 4827 5883 6929 4827
Size: 1056 1046 4827
Fragments arranged by size:
4827 1056 1046
BclI T'GATC_A
Cuts at: 3032 4449 3032
Size: 1417 5512
BfrBI ATG'CAT
Cuts at: 2322 2359 2322
Size: 37 6892
BglII A'GATC_T
Cuts at: 1383 1731 1851 2254 3891 4444 6481 1383
Size: 348 120 403 1637 553 2037 1831
Fragments arranged by size:
2037 1831 1637 553 403 348 120
BlpI GC'TnA_GC
Cuts at: 116 4041 116
Size: 3925 3004
Bme1580I G_kGCm'C
Cuts at: 779 1929 3515 3569 4554 4647 5771 779
Size: 1150 1586 54 985 93 1124 1937
Fragments arranged by size:
1937 1586 1150 1124 985 93 54
BmgBI CAC'GTC
Cuts at: 3922 3922
Size: 6929
BmrI ACTGGGnnnn_n'
Cuts at: 3583 4141 4222 4557 6701 3583
Size: 558 81 335 2144 3811
Fragments arranged by size:
3811 2144 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1139 3872 5296 1139
Size: 2733 1424 2772
Fragments arranged by size:
2772 2733 1424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1475 2243 2248 2366 2404 3812 1213
Size: 262 768 5 118 38 1408 4330
Fragments arranged by size:
4330 1408 768 262 118 38 5
Bpu10I CC'TnA_GC
Cuts at: 1353 2038 2846 3556 1353
Size: 685 808 710 4726
Fragments arranged by size:
4726 808 710 685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 1376 1687 1783 4105 4984 5698 5996 588
Size: 788 311 96 2322 879 714 298 1521
Fragments arranged by size:
2322 1521 879 788 714 311 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 6633 6633
Size: 6929
BsaAI yAC'GTr
Cuts at: 4282 4282
Size: 6929
BsaBI GATnn'nnATC
Cuts at: 6 2551 4443 6480 6
Size: 2545 1892 2037 455
Fragments arranged by size:
2545 2037 1892 455
BsaHI Gr'CG_yC
Cuts at: 4360 4608 5310 6806 4360
Size: 248 702 1496 4483
Fragments arranged by size:
4483 1496 702 248
BsaWI w'CCGG_w
Cuts at: 561 2432 4118 4639 5317 5728 5875 6149 561
Size: 1871 1686 521 678 411 147 274 1341
Fragments arranged by size:
1871 1686 1341 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3906 3936 3906
Size: 30 6899
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 880 2405 3295 3334 3930 880
Size: 1525 890 39 596 3879
Fragments arranged by size:
3879 1525 890 596 39
BseYI C'CCAG_C
Cuts at: 1542 3822 4354 5112 5777 6185 1542
Size: 2280 532 758 665 408 2286
Fragments arranged by size:
2286 2280 758 665 532 408
BsiEI CG_ry'CG
Cuts at: 104 4471 4517 5747 104
Size: 4367 46 1230 1286
Fragments arranged by size:
4367 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 779 1229 1280 2615 3515 3882 3943 4721 4911
Size: 450 51 1335 900 367 61 778 190
Cuts at: 4911 5771 779
Size: 860 1937
Fragments arranged by size:
1937 1335 900 860 778 450 367 190
61 51
BsmI GAATG_Cn'
Cuts at: 1256 2361 1256
Size: 1105 5824
BsmBI CGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 6929
Bsp1286I G_dGCh'C
Cuts at: 779 1229 1280 1929 2615 3515 3569 3882 3943
Size: 450 51 649 686 900 54 313 61
Cuts at: 3943 4554 4647 4721 4911 4973 5771 779
Size: 611 93 74 190 62 798 1937
Fragments arranged by size:
1937 900 798 686 649 611 450 313
190 93 74 62 61 54 51
BspEI T'CCGG_A
Cuts at: 561 2432 561
Size: 1871 5058
BspHI T'CATG_A
Cuts at: 5361 5361
Size: 6929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3093 3979 4193 4495 4876 3093
Size: 886 214 302 381 5146
Fragments arranged by size:
5146 886 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5221 5275 6759 6924 50
Size: 5171 54 1484 165 55
Fragments arranged by size:
5171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 710 1877 1990 2324 4841 710
Size: 1167 113 334 2517 2798
Fragments arranged by size:
2798 2517 1167 334 113
BsrFI r'CCGG_y
Cuts at: 4927 5108 4927
Size: 181 6748
BsrGI T'GTAC_A
Cuts at: 2444 2444
Size: 6929
BssSI C'ACGA_G
Cuts at: 5200 5908 5200
Size: 708 6221
BstAPI GCAn_nnn'nTGC
Cuts at: 2319 3886 4306 2319
Size: 1567 420 4942
Fragments arranged by size:
4942 1567 420
BstBI TT'CG_AA
Cuts at: 5290 5290
Size: 6929
BstEII G'GTnAC_C
Cuts at: 2463 2463
Size: 6929
BstYI r'GATC_y
Cuts at: 1 1383 1731 1789 1851 2022 2254 2451 2633
Size: 1382 348 58 62 171 232 197 182
Cuts at: 2633 3659 3891 3954 4421 4444 4779 5025 5331
Size: 1026 232 63 467 23 335 246 306
Cuts at: 5331 5343 5429 6481 1
Size: 12 86 1052 449
Fragments arranged by size:
1382 1052 1026 467 449 348 335 306
246 232 232 197 182 171 86 63
62 58 23 12
Bsu36I CC'TnA_GG
Cuts at: 2272 2272
Size: 6929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4879 5060 5223 4879
Size: 181 163 6585
Fragments arranged by size:
6585 181 163
BtsI GCAGTG_nn'
Cuts at: 189 236 2158 2724 4172 189
Size: 47 1922 566 1448 2946
Fragments arranged by size:
2946 1922 1448 566 47
DraI TTT'AAA
Cuts at: 1175 1557 2171 3928 1175
Size: 382 614 1757 4176
Fragments arranged by size:
4176 1757 614 382
DraIII CAC_nnn'GTG
Cuts at: 2643 2643
Size: 6929
DrdI GACnn_nn'nnGTC
Cuts at: 4635 5979 4635
Size: 1344 5585
EaeI y'GGCC_r
Cuts at: 2895 4514 4688 5079 5106 6451 2895
Size: 1619 174 391 27 1345 3373
Fragments arranged by size:
3373 1619 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4514 4514
Size: 6929
EarI CTCTTCn'nnn_
Cuts at: 274 371 401 419 1936 3191 3272 3311 3320
Size: 97 30 18 1517 1255 81 39 9
Cuts at: 3320 3371 3722 4952 5162 274
Size: 51 351 1230 210 2041
Fragments arranged by size:
2041 1517 1255 1230 351 210 97 81
51 39 30 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 207 5137 5879 6025 6739 207
Size: 4930 742 146 714 397
Fragments arranged by size:
4930 742 714 397 146
EcoICRI GAG'CTC
Cuts at: 1227 1278 3941 1227
Size: 51 2663 4215
Fragments arranged by size:
4215 2663 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 573 814 1213 1475 1748 2243 2248 2366
Size: 286 241 399 262 273 495 5 118
Cuts at: 2366 2404 3812 4754 5186 5539 6201 287
Size: 38 1408 942 432 353 662 1015
Fragments arranged by size:
1408 1015 942 662 495 432 399 353
286 273 262 241 118 38 5
EcoO109I rG'GnC_Cy
Cuts at: 261 3565 3566 4068 261
Size: 3304 1 502 3122
Fragments arranged by size:
3304 3122 502 1
EcoRI G'AATT_C
Cuts at: 3124 3933 3124
Size: 809 6120
EcoRV GAT'ATC
Cuts at: 228 3762 228
Size: 3534 3395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4948 4948
Size: 6929
FspI TGC'GCA
Cuts at: 4710 4710
Size: 6929
HaeII r_GCGC'y
Cuts at: 3822 4363 4611 5841 6612 3822
Size: 541 248 1230 771 4139
Fragments arranged by size:
4139 1230 771 541 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 658 690 991 1023 1125 1157 1781
Size: 32 280 32 301 32 102 32 624
Cuts at: 1781 1813 2597 2629 3050 3082 3145 3177 3583
Size: 32 784 32 421 32 63 32 406
Cuts at: 3583 3615 4771 4801 4803 4833 346
Size: 32 1156 30 2 30 2442
Fragments arranged by size:
2442 1156 784 624 421 406 301 280
102 63 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1045 1845 3629 3968 1045
Size: 800 1784 339 4006
Fragments arranged by size:
4006 1784 800 339
HindIII A'AGCT_T
Cuts at: 506 506
Size: 6929
HpaI GTT'AAC
Cuts at: 3629 3629
Size: 6929
KasI G'GCGC_C
Cuts at: 4359 4607 4359
Size: 248 6681
KpnI G_GTAC'C
Cuts at: 3949 3949
Size: 6929
MluI A'CGCG_T
Cuts at: 6362 6362
Size: 6929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1616 1652 1934 5688 5872 1616
Size: 36 282 3754 184 2673
Fragments arranged by size:
3754 2673 282 184 36
MscI TGG'CCA
Cuts at: 2897 4690 2897
Size: 1793 5136
MslI CAynn'nnrTG
Cuts at: 1016 1888 2327 4918 5045 1016
Size: 872 439 2591 127 2900
Fragments arranged by size:
2900 2591 872 439 127
MspA1I CmG'CkG
Cuts at: 115 460 2386 2767 3555 4040 4354 4714 5498
Size: 345 1926 381 788 485 314 360 784
Cuts at: 5498 5743 115
Size: 245 1301
Fragments arranged by size:
1926 1301 788 784 485 381 360 345
314 245
NaeI GCC'GGC
Cuts at: 5110 5110
Size: 6929
NarI GG'CG_CC
Cuts at: 4360 4608 4360
Size: 248 6681
NgoMIV G'CCGG_C
Cuts at: 5108 5108
Size: 6929
NsiI A_TGCA'T
Cuts at: 2324 2361 2324
Size: 37 6892
NspI r_CATG'y
Cuts at: 2007 2219 2237 2894 3421 3982 4482 6085 2007
Size: 212 18 657 527 561 500 1603 2851
Fragments arranged by size:
2851 1603 657 561 527 500 212 18
PciI A'CATG_T
Cuts at: 2233 2890 6081 2233
Size: 657 3191 3081
Fragments arranged by size:
3191 3081 657
PflMI CCAn_nnn'nTGG
Cuts at: 2643 2643
Size: 6929
PfoI T'CCnGG_A
Cuts at: 2420 2420
Size: 6929
PmeI GTTT'AAAC
Cuts at: 3928 3928
Size: 6929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5375 5407 5375
Size: 32 6897
PspOMI G'GGCC_C
Cuts at: 3565 3565
Size: 6929
PstI C_TGCA'G
Cuts at: 184 1101 3606 3976 4661 184
Size: 917 2505 370 685 2452
Fragments arranged by size:
2505 2452 917 685 370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6929
PvuII CAG'CTG
Cuts at: 115 460 2386 2767 3555 4354 4714 115
Size: 345 1926 381 788 799 360 2330
Fragments arranged by size:
2330 1926 799 788 381 360 345
RsrII CG'GwC_CG
Cuts at: 5124 5124
Size: 6929
SacI G_AGCT'C
Cuts at: 1229 1280 3943 1229
Size: 51 2663 4215
Fragments arranged by size:
4215 2663 51
SalI G'TCGA_C
Cuts at: 1043 3966 1043
Size: 2923 4006
SapI GCTCTTCn'nnn_
Cuts at: 419 4952 5162 419
Size: 4533 210 2186
Fragments arranged by size:
4533 2186 210
SbfI CC_TGCA'GG
Cuts at: 3976 3976
Size: 6929
ScaI AGT'ACT
Cuts at: 1183 2902 6446 1183
Size: 1719 3544 1666
Fragments arranged by size:
3544 1719 1666
SexAI A'CCwGG_T
Cuts at: 1443 1443
Size: 6929
SfcI C'TryA_G
Cuts at: 33 180 722 1025 1097 1822 3481 3602 3972
Size: 147 542 303 72 725 1659 121 370
Cuts at: 3972 4657 5625 5816 33
Size: 685 968 191 1146
Fragments arranged by size:
1659 1146 968 725 685 542 370 303
191 147 121 72
SfoI GGC'GCC
Cuts at: 4361 4609 4361
Size: 248 6681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
SmaI CCC'GGG
Cuts at: 3951 3951
Size: 6929
SmlI C'TyrA_G
Cuts at: 603 1391 1666 1798 3412 4084 4999 5713 5975
Size: 788 275 132 1614 672 915 714 262
Cuts at: 5975 603
Size: 1557
Fragments arranged by size:
1614 1557 915 788 714 672 275 262
132
SpeI A'CTAG_T
Cuts at: 67 2607 67
Size: 2540 4389
SphI G_CATG'C
Cuts at: 2219 3982 4482 2219
Size: 1763 500 4666
Fragments arranged by size:
4666 1763 500
SspI AAT'ATT
Cuts at: 987 2085 987
Size: 1098 5831
StuI AGG'CCT
Cuts at: 3143 3143
Size: 6929
StyI C'CwwG_G
Cuts at: 1078 4063 4417 1078
Size: 2985 354 3590
Fragments arranged by size:
3590 2985 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4512 4645 5104 5313 4512
Size: 133 459 209 6128
Fragments arranged by size:
6128 459 209 133
TatI w'GTAC_w
Cuts at: 1181 2283 2444 2900 6444 1181
Size: 1102 161 456 3544 1666
Fragments arranged by size:
3544 1666 1102 456 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3906 3938 6446 6478 3906
Size: 32 2508 32 4357
Fragments arranged by size:
4357 2508 32 32
Tth111I GACn'n_nGTC
Cuts at: 4726 4726
Size: 6929
XbaI T'CTAG_A
Cuts at: 1680 3960 1680
Size: 2280 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 685 2286 2626 2644 4424 685
Size: 1601 340 18 1780 3190
Fragments arranged by size:
3190 1780 1601 340 18
XmaI C'CCGG_G
Cuts at: 3949 3949
Size: 6929
XmnI GAAnn'nnTTC
Cuts at: 488 2663 488
Size: 2175 4754
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AloI AscI AvrII
BaeI BglI BmtI BsgI BsiWI BssHII BstXI BstZ17I
BtgI ClaI CspCI EcoNI FseI FspAI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI