Restriction map for
pF1KA1109
(Circular) (Six-base) MAPSORT of: pF1KA1109.seq Check: 9027 from: 1 to: 12161
pF1KA1109 12161 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1393 1393
Size: 12161
AatII G_ACGT'C
Cuts at: 6978 6978
Size: 12161
AccI GT'mk_AC
Cuts at: 6528 7915 9199 6528
Size: 1387 1284 9490
Fragments arranged by size:
9490 1387 1284
Acc65I G'GTAC_C
Cuts at: 9177 9177
Size: 12161
AclI AA'CG_TT
Cuts at: 4854 7382 12001 4854
Size: 2528 4619 5014
Fragments arranged by size:
5014 4619 2528
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2303 2563 2812 3893 4256 4700 5084 5300
Size: 84 260 249 1081 363 444 384 216
Cuts at: 5300 6031 6701 7238 7664 7674 7696 8237 8671
Size: 731 670 537 426 10 22 541 434
Cuts at: 8671 9986 10418 11165 11433 2219
Size: 1315 432 747 268 2947
Fragments arranged by size:
2947 1315 1081 747 731 670 541 537
444 434 432 426 384 363 268 260
249 216 84 22 10
AflII C'TTAA_G
Cuts at: 2569 5023 7705 2569
Size: 2454 2682 7025
Fragments arranged by size:
7025 2682 2454
AflIII A'CryG_T
Cuts at: 2365 9153 9513 10617 11594 2365
Size: 6788 360 1104 977 2932
Fragments arranged by size:
6788 2932 1104 977 360
AgeI A'CCGG_T
Cuts at: 10549 10549
Size: 12161
AhdI GACnn_n'nnGTC
Cuts at: 3799 5163 5304 3799
Size: 1364 141 10656
Fragments arranged by size:
10656 1364 141
AleI CACnn'nnGTG
Cuts at: 184 8497 8699 184
Size: 8313 202 3646
Fragments arranged by size:
8313 3646 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5728 5728
Size: 12161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6774 6806 6774
Size: 32 12129
AlwNI CAG_nnn'CTG
Cuts at: 645 4450 4711 6724 9466 11033 645
Size: 3805 261 2013 2742 1567 1773
Fragments arranged by size:
3805 2742 2013 1773 1567 261
ApaI G_GGCC'C
Cuts at: 8178 8178
Size: 12161
ApaLI G'TGCA_C
Cuts at: 1728 3179 10931 1728
Size: 1451 7752 2958
Fragments arranged by size:
7752 2958 1451
ApoI r'AATT_y
Cuts at: 155 323 742 2106 3028 3076 3593 4163 4364
Size: 168 419 1364 922 48 517 570 201
Cuts at: 4364 4611 4847 6304 6929 7002 7619 7873 8154
Size: 247 236 1457 625 73 617 254 281
Cuts at: 8154 8505 9165 11514 11770 155
Size: 351 660 2349 256 546
Fragments arranged by size:
2349 1457 1364 922 660 625 617 570
546 517 419 351 281 256 254 247
236 201 168 73 48
AseI AT'TA_AT
Cuts at: 20 1678 8944 20
Size: 1658 7266 3237
Fragments arranged by size:
7266 3237 1658
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12161
AvaI C'yCGr_G
Cuts at: 5048 9181 5048
Size: 4133 8028
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4296 4329 4296
Size: 33 12128
BamHI G'GATC_C
Cuts at: 1490 2549 4521 9060 9186 1490
Size: 1059 1972 4539 126 4465
Fragments arranged by size:
4539 4465 1972 1059 126
BanI G'GyrC_C
Cuts at: 4408 6799 9177 9591 9839 9874 4408
Size: 2391 2378 414 248 35 6695
Fragments arranged by size:
6695 2391 2378 414 248 35
BanII G_rGCy'C
Cuts at: 2353 5072 6713 7669 7911 8178 8723 9175 10205
Size: 2719 1641 956 242 267 545 452 1030
Cuts at: 10205 2353
Size: 4309
Fragments arranged by size:
4309 2719 1641 1030 956 545 452 267
242
BbeI G_GCGC'C
Cuts at: 9595 9843 9595
Size: 248 11913
BbsI GAAGACnn'nnnn_
Cuts at: 703 1264 2309 2800 4592 4704 5019 5300 5354
Size: 561 1045 491 1792 112 315 281 54
Cuts at: 5354 6224 703
Size: 870 6640
Fragments arranged by size:
6640 1792 1045 870 561 491 315 281
112 54
BbvCI CC'TCA_GC
Cuts at: 1311 2450 2846 3035 1311
Size: 1139 396 189 10437
Fragments arranged by size:
10437 1139 396 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 12127
BciVI GTATCCnnnnn_n'
Cuts at: 1497 4108 4324 5124 6843 7684 10059 10820 12161
Size: 2611 216 800 1719 841 2375 761 1341
Cuts at: 12161 1497
Size: 1497
Fragments arranged by size:
2611 2375 1719 1497 1341 841 800 761
216
BclI T'GATC_A
Cuts at: 8250 9681 8250
Size: 1431 10730
BfrBI ATG'CAT
Cuts at: 205 344 6338 6599 205
Size: 139 5994 261 5767
Fragments arranged by size:
5994 5767 261 139
BglI GCCn_nnn'nGGC
Cuts at: 6518 6518
Size: 12161
BglII A'GATC_T
Cuts at: 190 2119 7842 9676 11713 190
Size: 1929 5723 1834 2037 638
Fragments arranged by size:
5723 2037 1929 1834 638
BlpI GC'TnA_GC
Cuts at: 638 8717 9273 638
Size: 8079 556 3526
Fragments arranged by size:
8079 3526 556
Bme1580I G_kGCm'C
Cuts at: 1732 1755 2781 3183 8178 9786 9879 10935 1732
Size: 23 1026 402 4995 1608 93 1056 2958
Fragments arranged by size:
4995 2958 1608 1056 1026 402 93 23
BmgBI CAC'GTC
Cuts at: 976 976
Size: 12161
BmrI ACTGGGnnnn_n'
Cuts at: 7604 9373 9454 9789 11933 7604
Size: 1769 81 335 2144 7832
Fragments arranged by size:
7832 2144 1769 335 81
BmtI G_CTAG'C
Cuts at: 4718 6476 4718
Size: 1758 10403
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10528 10528
Size: 12161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 857 944 2704 4123 4226 5228 5817 6412
Size: 753 87 1760 1419 103 1002 589 595
Cuts at: 6412 6455 7852 8042 8229 8916 104
Size: 43 1397 190 187 687 3349
Fragments arranged by size:
3349 1760 1419 1397 1002 753 687 595
589 190 187 103 87 43
Bpu10I CC'TnA_GC
Cuts at: 1311 2450 2846 3035 1311
Size: 1139 396 189 10437
Fragments arranged by size:
10437 1139 396 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 339 1681 6603 6775 7653 9337 10216 10708
Size: 74 1342 4922 172 878 1684 879 492
Cuts at: 10708 11006 265
Size: 298 1420
Fragments arranged by size:
4922 1684 1420 1342 879 878 492 298
172 74
BsaI GGTCTCn'nnnn_
Cuts at: 4202 6983 11865 4202
Size: 2781 4882 4498
Fragments arranged by size:
4882 4498 2781
BsaAI yAC'GTr
Cuts at: 9514 9514
Size: 12161
BsaBI GATnn'nnATC
Cuts at: 6 3779 3839 9675 11712 6
Size: 3773 60 5836 2037 455
Fragments arranged by size:
5836 3773 2037 455 60
BsaHI Gr'CG_yC
Cuts at: 6975 8367 9592 9840 10542 12038 6975
Size: 1392 1225 248 702 1496 7098
Fragments arranged by size:
7098 1496 1392 1225 702 248
BsaWI w'CCGG_w
Cuts at: 9350 9871 10549 10823 10970 11381 9350
Size: 521 678 274 147 411 10130
Fragments arranged by size:
10130 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 942 972 3249 3279 4647 4677 4911 4941 5765
Size: 30 2277 30 1368 30 234 30 824
Cuts at: 5765 5795 5892 5922 7275 7305 7523 7553 942
Size: 30 97 30 1353 30 218 30 5550
Fragments arranged by size:
5550 2277 1368 1353 824 234 218 97
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 107 1064 3589 5158 5897 5906 6023 6398
Size: 3 957 2525 1569 739 9 117 375
Cuts at: 6398 6721 7547 8546 9065 104
Size: 323 826 999 519 3200
Fragments arranged by size:
3200 2525 1569 999 957 826 739 519
375 323 117 9 3
BseYI C'CCAG_C
Cuts at: 979 5966 7218 9586 10344 10921 11417 979
Size: 4987 1252 2368 758 577 496 1723
Fragments arranged by size:
4987 2368 1723 1252 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1036 4172 9092 463
Size: 573 3136 4920 3532
Fragments arranged by size:
4920 3532 3136 573
BsiEI CG_ry'CG
Cuts at: 104 9703 9749 10957 104
Size: 9599 46 1208 1308
Fragments arranged by size:
9599 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1732 3183 5072 7911 8723 9175 9953 10143 10935
Size: 1451 1889 2839 812 452 778 190 792
Cuts at: 10935 1732
Size: 2958
Fragments arranged by size:
2958 2839 1889 1451 812 792 778 452
190
BsmI GAATG_Cn'
Cuts at: 905 4392 5264 6340 8093 905
Size: 3487 872 1076 1753 4973
Fragments arranged by size:
4973 3487 1753 1076 872
BsmBI CGTCTCn'nnnn_
Cuts at: 5402 5402
Size: 12161
Bsp1286I G_dGCh'C
Cuts at: 1732 1755 2353 2781 3183 5072 6713 7669 7911
Size: 23 598 428 402 1889 1641 956 242
Cuts at: 7911 8178 8723 9175 9786 9879 9953 10143 10205
Size: 267 545 452 611 93 74 190 62
Cuts at: 10205 10935 1732
Size: 730 2958
Fragments arranged by size:
2958 1889 1641 956 730 611 598 545
452 428 402 267 242 190 93 74
62 23
BspEI T'CCGG_A
Cuts at: 11381 11381
Size: 12161
BspHI T'CATG_A
Cuts at: 1764 3491 8736 11337 1764
Size: 1727 5245 2601 2588
Fragments arranged by size:
5245 2601 2588 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1393 9211 9425 9727 10108 1393
Size: 7818 214 302 381 3446
Fragments arranged by size:
7818 3446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 10453 10507 11991 12156 50
Size: 10403 54 1484 165 55
Fragments arranged by size:
10403 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4598 6052 10073 4598
Size: 1454 4021 6686
Fragments arranged by size:
6686 4021 1454
BsrFI r'CCGG_y
Cuts at: 803 5701 10159 10340 10549 803
Size: 4898 4458 181 209 2415
Fragments arranged by size:
4898 4458 2415 209 181
BsrGI T'GTAC_A
Cuts at: 375 3477 8268 375
Size: 3102 4791 4268
Fragments arranged by size:
4791 4268 3102
BssSI C'ACGA_G
Cuts at: 7649 10432 10790 7649
Size: 2783 358 9020
Fragments arranged by size:
9020 2783 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4711 9538 4711
Size: 4827 7334
BstBI TT'CG_AA
Cuts at: 2880 10522 2880
Size: 7642 4519
BstEII G'GTnAC_C
Cuts at: 8307 8307
Size: 12161
BstXI CCAn_nnnn'nTGG
Cuts at: 4123 6950 4123
Size: 2827 9334
BstYI r'GATC_y
Cuts at: 1 190 1490 1939 2119 2549 4521 5839 6195
Size: 189 1300 449 180 430 1972 1318 356
Cuts at: 6195 7842 9060 9186 9653 9676 10011 10257 11269
Size: 1647 1218 126 467 23 335 246 1012
Cuts at: 11269 11355 11367 11713 1
Size: 86 12 346 449
Fragments arranged by size:
1972 1647 1318 1300 1218 1012 467 449
449 430 356 346 335 246 189 180
126 86 23 12
BtgI C'CryG_G
Cuts at: 2553 7336 7785 8388 2553
Size: 4783 449 603 6326
Fragments arranged by size:
6326 4783 603 449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 536 10111 10292 10455 536
Size: 9575 181 163 2242
Fragments arranged by size:
9575 2242 181 163
BtsI GCAGTG_nn'
Cuts at: 233 583 944 1092 1797 4259 6590 9404 233
Size: 350 361 148 705 2462 2331 2814 2990
Fragments arranged by size:
2990 2814 2462 2331 705 361 350 148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 687 722 1193 1228 1266 1301 2147 2182 8301
Size: 35 471 35 38 35 846 35 6119
Cuts at: 8301 8336 687
Size: 35 4512
Fragments arranged by size:
6119 4512 846 471 38 35 35 35
35 35
DraI TTT'AAA
Cuts at: 293 612 3779 3858 6903 8598 9160 293
Size: 319 3167 79 3045 1695 562 3294
Fragments arranged by size:
3294 3167 3045 1695 562 319 79
DrdI GACnn_nn'nnGTC
Cuts at: 1268 8819 9867 10725 1268
Size: 7551 1048 858 2704
Fragments arranged by size:
7551 2704 1048 858
EaeI y'GGCC_r
Cuts at: 3641 5452 6519 9746 9920 10311 10338 11683 3641
Size: 1811 1067 3227 174 391 27 1345 4119
Fragments arranged by size:
4119 3227 1811 1345 1067 391 174 27
EagI C'GGCC_G
Cuts at: 9746 9746
Size: 12161
EarI CTCTTCn'nnn_
Cuts at: 1478 2539 4275 4890 5060 5276 6255 7260 7435
Size: 1061 1736 615 170 216 979 1005 175
Cuts at: 7435 8438 10184 10394 1478
Size: 1003 1746 210 3245
Fragments arranged by size:
3245 1746 1736 1061 1005 1003 979 615
216 210 175 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7931 10369 10679 10825 11971 7931
Size: 2438 310 146 1146 8121
Fragments arranged by size:
8121 2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5070 7909 8721 9173 5070
Size: 2839 812 452 8058
Fragments arranged by size:
8058 2839 812 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 857 944 2219 2303 2563 2704 2812 3893
Size: 753 87 1275 84 260 141 108 1081
Cuts at: 3893 4123 4226 4256 4700 5084 5228 5300 5817
Size: 230 103 30 444 384 144 72 517
Cuts at: 5817 6031 6412 6455 6701 7238 7664 7674 7696
Size: 214 381 43 246 537 426 10 22
Cuts at: 7696 7852 8042 8229 8237 8671 8916 9986 10418
Size: 156 190 187 8 434 245 1070 432
Cuts at: 10418 11165 11433 104
Size: 747 268 832
Fragments arranged by size:
1275 1081 1070 832 753 747 537 517
444 434 432 426 384 381 268 260
246 245 230 214 190 187 156 144
141 108 103 87 84 72 43 30
22 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 6680 6680
Size: 12161
EcoO109I rG'GnC_Cy
Cuts at: 7331 8175 9300 7331
Size: 844 1125 10192
Fragments arranged by size:
10192 1125 844
EcoRI G'AATT_C
Cuts at: 2106 8505 9165 2106
Size: 6399 660 5102
Fragments arranged by size:
6399 5102 660
EcoRV GAT'ATC
Cuts at: 4767 7823 4767
Size: 3056 9105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 703 2249 3943 7100 10180 703
Size: 1546 1694 3157 3080 2684
Fragments arranged by size:
3157 3080 2684 1694 1546
FspI TGC'GCA
Cuts at: 9942 9942
Size: 12161
HaeII r_GCGC'y
Cuts at: 9595 9843 10865 11844 9595
Size: 248 1022 979 9912
Fragments arranged by size:
9912 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1132 1162 1164 1194 1356 1388 2400 2432 3785
Size: 30 2 30 162 32 1012 32 1353
Cuts at: 3785 3817 3881 3913 4645 4677 5080 5112 5149
Size: 32 64 32 732 32 403 32 37
Cuts at: 5149 5181 5290 5322 5598 5619 5630 5651 6396
Size: 32 109 32 276 21 11 21 745
Cuts at: 6396 6412 6428 6444 6874 6906 7563 7595 8205
Size: 16 16 16 430 32 657 32 610
Cuts at: 8205 8237 8584 8616 8731 8763 8833 8865 10003
Size: 32 347 32 115 32 70 32 1138
Cuts at: 10003 10033 10035 10065 1132
Size: 30 2 30 3228
Fragments arranged by size:
3228 1353 1138 1012 745 732 657 610
430 403 347 276 162 115 109 70
64 37 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 30 30 21 21 16 16
16 11 2 2
HincII GTy'rAC
Cuts at: 143 3257 6529 7832 9200 143
Size: 3114 3272 1303 1368 3104
Fragments arranged by size:
3272 3114 3104 1368 1303
HindIII A'AGCT_T
Cuts at: 1038 3722 7082 7708 1038
Size: 2684 3360 626 5491
Fragments arranged by size:
5491 3360 2684 626
KasI G'GCGC_C
Cuts at: 9591 9839 9591
Size: 248 11913
KpnI G_GTAC'C
Cuts at: 9181 9181
Size: 12161
MfeI C'AATT_G
Cuts at: 3947 3947
Size: 12161
MluI A'CGCG_T
Cuts at: 11594 11594
Size: 12161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1324 2320 7521 10832 11016 951
Size: 373 996 5201 3311 184 2096
Fragments arranged by size:
5201 3311 2096 996 373 184
MscI TGG'CCA
Cuts at: 5454 9922 5454
Size: 4468 7693
MslI CAynn'nnrTG
Cuts at: 184 200 674 680 1141 2192 2633 3250 6632
Size: 16 474 6 461 1051 441 617 3382
Cuts at: 6632 7132 8136 8497 8699 8701 10150 10277 184
Size: 500 1004 361 202 2 1449 127 2068
Fragments arranged by size:
3382 2068 1449 1051 1004 617 500 474
461 441 361 202 127 16 6 2
MspA1I CmG'CkG
Cuts at: 219 247 2239 4450 6151 9272 9586 9946 10959
Size: 28 1992 2211 1701 3121 314 360 1013
Cuts at: 10959 11204 219
Size: 245 1176
Fragments arranged by size:
3121 2211 1992 1701 1176 1013 360 314
245 28
NaeI GCC'GGC
Cuts at: 10342 10342
Size: 12161
NarI GG'CG_CC
Cuts at: 9592 9840 9592
Size: 248 11913
NcoI C'CATG_G
Cuts at: 2553 7336 7785 8388 2553
Size: 4783 449 603 6326
Fragments arranged by size:
6326 4783 603 449
NdeI CA'TA_TG
Cuts at: 2189 4061 6633 7166 8126 2189
Size: 1872 2572 533 960 6224
Fragments arranged by size:
6224 2572 1872 960 533
NgoMIV G'CCGG_C
Cuts at: 10340 10340
Size: 12161
NheI G'CTAG_C
Cuts at: 4714 6472 4714
Size: 1758 10403
NsiI A_TGCA'T
Cuts at: 207 346 6340 6601 207
Size: 139 5994 261 5767
Fragments arranged by size:
5994 5767 261 139
NspI r_CATG'y
Cuts at: 205 970 1840 2369 2458 4268 6342 6599 6603
Size: 765 870 529 89 1810 2074 257 4
Cuts at: 6603 7137 7494 8141 9214 9714 10621 205
Size: 534 357 647 1073 500 907 1745
Fragments arranged by size:
2074 1810 1745 1073 907 870 765 647
534 529 500 357 257 89 4
PasI CC'CwG_GG
Cuts at: 7021 7021
Size: 12161
PciI A'CATG_T
Cuts at: 2365 10617 2365
Size: 8252 3909
PflMI CCAn_nnn'nTGG
Cuts at: 250 7015 8083 8389 250
Size: 6765 1068 306 4022
Fragments arranged by size:
6765 4022 1068 306
PfoI T'CCnGG_A
Cuts at: 1061 5100 5126 5244 5623 5769 7868 1061
Size: 4039 26 118 379 146 2099 5354
Fragments arranged by size:
5354 4039 2099 379 146 118 26
PmeI GTTT'AAAC
Cuts at: 9160 9160
Size: 12161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11300 11332 11300
Size: 32 12129
PpuMI rG'GwC_Cy
Cuts at: 7331 7331
Size: 12161
PsiI TTA'TAA
Cuts at: 1787 4004 1787
Size: 2217 9944
PspOMI G'GGCC_C
Cuts at: 8174 8174
Size: 12161
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1110 1142 5507 5539 1110
Size: 32 4365 32 7732
Fragments arranged by size:
7732 4365 32 32
PstI C_TGCA'G
Cuts at: 5988 6420 9208 9893 5988
Size: 432 2788 685 8256
Fragments arranged by size:
8256 2788 685 432
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12161
PvuII CAG'CTG
Cuts at: 219 247 2239 4450 6151 9586 9946 219
Size: 28 1992 2211 1701 3435 360 2434
Fragments arranged by size:
3435 2434 2211 1992 1701 360 28
RsrII CG'GwC_CG
Cuts at: 10356 10356
Size: 12161
SacI G_AGCT'C
Cuts at: 5072 7911 8723 9175 5072
Size: 2839 812 452 8058
Fragments arranged by size:
8058 2839 812 452
SalI G'TCGA_C
Cuts at: 6527 9198 6527
Size: 2671 9490
SanDI GG'GwC_CC
Cuts at: 7331 7331
Size: 12161
SapI GCTCTTCn'nnn_
Cuts at: 4275 5060 7435 8438 10184 10394 4275
Size: 785 2375 1003 1746 210 6042
Fragments arranged by size:
6042 2375 1746 1003 785 210
SbfI CC_TGCA'GG
Cuts at: 9208 9208
Size: 12161
ScaI AGT'ACT
Cuts at: 4251 11678 4251
Size: 7427 4734
SexAI A'CCwGG_T
Cuts at: 813 4092 6489 813
Size: 3279 2397 6485
Fragments arranged by size:
6485 3279 2397
SfcI C'TryA_G
Cuts at: 33 2354 2735 2772 2933 4433 5492 5725 5984
Size: 2321 381 37 161 1500 1059 233 259
Cuts at: 5984 6260 6416 9112 9204 9889 10882 11073 33
Size: 276 156 2696 92 685 993 191 1121
Fragments arranged by size:
2696 2321 1500 1121 1059 993 685 381
276 259 233 191 161 156 92 37
SfoI GGC'GCC
Cuts at: 9593 9841 9593
Size: 248 11913
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12161
SmaI CCC'GGG
Cuts at: 9183 9183
Size: 12161
SmlI C'TyrA_G
Cuts at: 280 318 1660 2569 5023 5048 6582 6754 7668
Size: 38 1342 909 2454 25 1534 172 914
Cuts at: 7668 7705 9316 10231 10723 10985 280
Size: 37 1611 915 492 262 1456
Fragments arranged by size:
2454 1611 1534 1456 1342 915 914 909
492 262 172 38 37 25
SpeI A'CTAG_T
Cuts at: 67 6222 7429 67
Size: 6155 1207 4799
Fragments arranged by size:
6155 4799 1207
SphI G_CATG'C
Cuts at: 4268 6599 9214 9714 4268
Size: 2331 2615 500 6715
Fragments arranged by size:
6715 2615 2331 500
SspI AAT'ATT
Cuts at: 2306 2646 5505 8202 2306
Size: 340 2859 2697 6265
Fragments arranged by size:
6265 2859 2697 340
StuI AGG'CCT
Cuts at: 4120 4120
Size: 12161
StyI C'CwwG_G
Cuts at: 1003 1422 2553 6984 7336 7785 8388 8578 9295
Size: 419 1131 4431 352 449 603 190 717
Cuts at: 9295 9649 1003
Size: 354 3515
Fragments arranged by size:
4431 3515 1131 717 603 449 419 354
352 190
SwaI ATTT'AAAT
Cuts at: 3779 3779
Size: 12161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 926 1305 5576 5704 7144 9744 9877 10336 10545
Size: 379 4271 128 1440 2600 133 459 209
Cuts at: 10545 926
Size: 2542
Fragments arranged by size:
4271 2600 2542 1440 459 379 209 133
128
TatI w'GTAC_w
Cuts at: 375 1529 2710 3477 4249 5553 8268 11676 375
Size: 1154 1181 767 772 1304 2715 3408 860
Fragments arranged by size:
3408 2715 1304 1181 1154 860 772 767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 942 974 3334 3366 4485 4517 5943 5975 7416
Size: 32 2360 32 1119 32 1426 32 1441
Cuts at: 7416 7448 11678 11710 942
Size: 32 4230 32 1393
Fragments arranged by size:
4230 2360 1441 1426 1393 1119 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 9958 9958
Size: 12161
XbaI T'CTAG_A
Cuts at: 2014 8925 9192 2014
Size: 6911 267 4983
Fragments arranged by size:
6911 4983 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 9656 9656
Size: 12161
XhoI C'TCGA_G
Cuts at: 5048 5048
Size: 12161
XmaI C'CCGG_G
Cuts at: 9181 9181
Size: 12161
XmnI GAAnn'nnTTC
Cuts at: 3487 6735 7269 3487
Size: 3248 534 8379
Fragments arranged by size:
8379 3248 534
ZraI GAC'GTC
Cuts at: 6976 6976
Size: 12161
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BssHII BstZ17I Bsu36I ClaI
DraIII FseI FspAI HpaI NotI NruI PacI PmlI
PshAI PspXI SacII SfiI SgrAI SnaBI SrfI