Restriction map for
pF1KA1017
(Circular) (Six-base) MAPSORT of: pF1KA1017.seq Check: 263 from: 1 to: 6158
pF1KA1017 6158 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 3196 3196
Size: 6158
Acc65I G'GTAC_C
Cuts at: 3174 3174
Size: 6158
AclI AA'CG_TT
Cuts at: 5998 5998
Size: 6158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1366 2119 2492 3983 4415 4768 5430 104
Size: 1262 753 373 1491 432 353 662 832
Fragments arranged by size:
1491 1262 832 753 662 432 373 353
AfeI AGC'GCT
Cuts at: 841 841
Size: 6158
AflIII A'CryG_T
Cuts at: 2043 3510 5310 5591 2043
Size: 1467 1800 281 2610
Fragments arranged by size:
2610 1800 1467 281
AlwNI CAG_nnn'CTG
Cuts at: 250 1750 2097 3059 3463 4901 250
Size: 1500 347 962 404 1438 1507
Fragments arranged by size:
1507 1500 1438 962 404 347
ApaI G_GGCC'C
Cuts at: 2989 2989
Size: 6158
ApaLI G'TGCA_C
Cuts at: 4996 4996
Size: 6158
ApoI r'AATT_y
Cuts at: 695 909 1033 1213 2016 2078 3162 5511 5767
Size: 214 124 180 803 62 1084 2349 256
Cuts at: 5767 695
Size: 1086
Fragments arranged by size:
2349 1086 1084 803 256 214 180 124
62
AseI AT'TA_AT
Cuts at: 20 20
Size: 6158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
AvaI C'yCGr_G
Cuts at: 3178 3178
Size: 6158
BamHI G'GATC_C
Cuts at: 619 2828 3183 619
Size: 2209 355 3594
Fragments arranged by size:
3594 2209 355
BanI G'GyrC_C
Cuts at: 988 1438 3174 3588 3836 3871 988
Size: 450 1736 414 248 35 3275
Fragments arranged by size:
3275 1736 450 414 248 35
BanII G_rGCy'C
Cuts at: 1456 1481 2989 3172 4202 1456
Size: 25 1508 183 1030 3412
Fragments arranged by size:
3412 1508 1030 183 25
BbeI G_GCGC'C
Cuts at: 3592 3840 3592
Size: 248 5910
BbsI GAAGACnn'nnnn_
Cuts at: 110 1166 1280 1547 1592 1954 110
Size: 1056 114 267 45 362 4314
Fragments arranged by size:
4314 1056 362 267 114 45
BciVI GTATCCnnnnn_n'
Cuts at: 153 2175 4056 5112 6158 153
Size: 2022 1881 1056 1046 153
Fragments arranged by size:
2022 1881 1056 1046 153
BclI T'GATC_A
Cuts at: 189 606 3678 189
Size: 417 3072 2669
Fragments arranged by size:
3072 2669 417
BglII A'GATC_T
Cuts at: 2250 3673 5710 2250
Size: 1423 2037 2698
Fragments arranged by size:
2698 2037 1423
BlpI GC'TnA_GC
Cuts at: 3270 3270
Size: 6158
Bme1580I G_kGCm'C
Cuts at: 2989 3783 3876 5000 2989
Size: 794 93 1124 4147
Fragments arranged by size:
4147 1124 794 93
BmrI ACTGGGnnnn_n'
Cuts at: 621 3370 3451 3786 5930 621
Size: 2749 81 335 2144 849
Fragments arranged by size:
2749 2144 849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4525 4525
Size: 6158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1562 2773 1277
Size: 285 1211 4662
Fragments arranged by size:
4662 1211 285
Bpu10I CC'TnA_GC
Cuts at: 1480 2975 1480
Size: 1495 4663
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2515 3052 3334 4213 4927 5225 2515
Size: 537 282 879 714 298 3448
Fragments arranged by size:
3448 879 714 537 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 1464 2830 5862 1464
Size: 1366 3032 1760
Fragments arranged by size:
3032 1760 1366
BsaAI yAC'GTr
Cuts at: 3511 3511
Size: 6158
BsaBI GATnn'nnATC
Cuts at: 6 3672 5709 6
Size: 3666 2037 455
Fragments arranged by size:
3666 2037 455
BsaHI Gr'CG_yC
Cuts at: 3589 3837 4539 6035 3589
Size: 248 702 1496 3712
Fragments arranged by size:
3712 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3347 3868 4546 4957 5104 5378 3347
Size: 521 678 411 147 274 4127
Fragments arranged by size:
4127 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1153 1183 2910 2940 1153
Size: 30 1727 30 4371
Fragments arranged by size:
4371 1727 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 1358 1564 544
Size: 814 206 5138
Fragments arranged by size:
5138 814 206
BseYI C'CCAG_C
Cuts at: 155 196 3143 3583 4341 5006 5414 155
Size: 41 2947 440 758 665 408 899
Fragments arranged by size:
2947 899 758 665 440 408 41
BsiEI CG_ry'CG
Cuts at: 104 3129 3700 3746 4976 104
Size: 3025 571 46 1230 1286
Fragments arranged by size:
3025 1286 1230 571 46
BsiHKAI G_wGCw'C
Cuts at: 483 1481 2887 3172 3950 4140 5000 483
Size: 998 1406 285 778 190 860 1641
Fragments arranged by size:
1641 1406 998 860 778 285 190
BsmI GAATG_Cn'
Cuts at: 1340 1340
Size: 6158
Bsp1286I G_dGCh'C
Cuts at: 483 1456 1481 2887 2989 3172 3783 3876 3950
Size: 973 25 1406 102 183 611 93 74
Cuts at: 3950 4140 4202 5000 483
Size: 190 62 798 1641
Fragments arranged by size:
1641 1406 973 798 611 190 183 102
93 74 62 25
BspHI T'CATG_A
Cuts at: 4590 4590
Size: 6158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1247 1534 2696 3067 3208 3422 3724 4105 1247
Size: 287 1162 371 141 214 302 381 3300
Fragments arranged by size:
3300 1162 381 371 302 287 214 141
BsrBI CCG'CTC
Cuts at: 50 4450 4504 5988 6153 50
Size: 4400 54 1484 165 55
Fragments arranged by size:
4400 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 917 1631 1863 1949 4070 917
Size: 714 232 86 2121 3005
Fragments arranged by size:
3005 2121 714 232 86
BsrFI r'CCGG_y
Cuts at: 4156 4337 4156
Size: 181 5977
BsrGI T'GTAC_A
Cuts at: 303 303
Size: 6158
BssSI C'ACGA_G
Cuts at: 1449 2886 4429 5137 1449
Size: 1437 1543 708 2470
Fragments arranged by size:
2470 1543 1437 708
BstAPI GCAn_nnn'nTGC
Cuts at: 250 672 3535 250
Size: 422 2863 2873
Fragments arranged by size:
2873 2863 422
BstBI TT'CG_AA
Cuts at: 4519 4519
Size: 6158
BstXI CCAn_nnnn'nTGG
Cuts at: 2580 2949 2580
Size: 369 5789
BstYI r'GATC_y
Cuts at: 1 619 2250 2828 3183 3650 3673 4008 4254
Size: 618 1631 578 355 467 23 335 246
Cuts at: 4254 4560 4572 4658 5710 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1631 1052 618 578 467 449 355 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3070 3070
Size: 6158
BtgI C'CryG_G
Cuts at: 984 2840 2981 984
Size: 1856 141 4161
Fragments arranged by size:
4161 1856 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4108 4289 4452 4108
Size: 181 163 5814
Fragments arranged by size:
5814 181 163
BtsI GCAGTG_nn'
Cuts at: 2552 3004 3401 2552
Size: 452 397 5309
Fragments arranged by size:
5309 452 397
DraI TTT'AAA
Cuts at: 1209 3157 1209
Size: 1948 4210
DrdI GACnn_nn'nnGTC
Cuts at: 3864 5208 3864
Size: 1344 4814
EaeI y'GGCC_r
Cuts at: 3743 3917 4308 4335 5680 3743
Size: 174 391 27 1345 4221
Fragments arranged by size:
4221 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3743 3743
Size: 6158
EarI CTCTTCn'nnn_
Cuts at: 132 858 1134 1226 1385 1499 2360 2757 4181
Size: 726 276 92 159 114 861 397 1424
Cuts at: 4181 4391 132
Size: 210 1899
Fragments arranged by size:
1899 1424 861 726 397 276 210 159
114 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4366 5108 5254 5968 4366
Size: 742 146 714 4556
Fragments arranged by size:
4556 742 714 146
EcoICRI GAG'CTC
Cuts at: 1479 3170 1479
Size: 1691 4467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1277 1366 1562 2119 2492 2773 3983 4415
Size: 1173 89 196 557 373 281 1210 432
Cuts at: 4415 4768 5430 104
Size: 353 662 832
Fragments arranged by size:
1210 1173 832 662 557 432 373 353
281 196 89
EcoO109I rG'GnC_Cy
Cuts at: 1236 1641 3095 3297 1236
Size: 405 1454 202 4097
Fragments arranged by size:
4097 1454 405 202
EcoRI G'AATT_C
Cuts at: 1213 2016 3162 1213
Size: 803 1146 4209
Fragments arranged by size:
4209 1146 803
EcoRV GAT'ATC
Cuts at: 830 2400 2731 3066 830
Size: 1570 331 335 3922
Fragments arranged by size:
3922 1570 335 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 748 956 1865 1928 2124 4177 748
Size: 208 909 63 196 2053 2729
Fragments arranged by size:
2729 2053 909 208 196 63
FspI TGC'GCA
Cuts at: 3939 3939
Size: 6158
HaeII r_GCGC'y
Cuts at: 843 3592 3840 5070 5841 843
Size: 2749 248 1230 771 1160
Fragments arranged by size:
2749 1230 1160 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1147 1153 1179 1185 1443 1463 1475 1480 1495
Size: 6 26 6 258 20 12 5 15
Cuts at: 1495 1512 1744 1776 2351 2383 2467 2499 2634
Size: 17 232 32 575 32 84 32 135
Cuts at: 2634 2666 4000 4030 4032 4062 1147
Size: 32 1334 30 2 30 3243
Fragments arranged by size:
3243 1334 575 258 232 135 84 32
32 32 32 30 30 26 20 17
15 12 6 6 5 2
HincII GTy'rAC
Cuts at: 291 3197 291
Size: 2906 3252
HindIII A'AGCT_T
Cuts at: 2149 2854 2149
Size: 705 5453
KasI G'GCGC_C
Cuts at: 3588 3836 3588
Size: 248 5910
KpnI G_GTAC'C
Cuts at: 3178 3178
Size: 6158
MluI A'CGCG_T
Cuts at: 5591 5591
Size: 6158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2806 4917 5101 2507
Size: 299 2111 184 3564
Fragments arranged by size:
3564 2111 299 184
MscI TGG'CCA
Cuts at: 3919 3919
Size: 6158
MslI CAynn'nnrTG
Cuts at: 112 659 800 1268 1887 2947 4147 4274 112
Size: 547 141 468 619 1060 1200 127 1996
Fragments arranged by size:
1996 1200 1060 619 547 468 141 127
MspA1I CmG'CkG
Cuts at: 247 616 978 2481 2640 3269 3583 3943 4727
Size: 369 362 1503 159 629 314 360 784
Cuts at: 4727 4972 247
Size: 245 1433
Fragments arranged by size:
1503 1433 784 629 369 362 360 314
245 159
NaeI GCC'GGC
Cuts at: 4339 4339
Size: 6158
NarI GG'CG_CC
Cuts at: 3589 3837 3589
Size: 248 5910
NcoI C'CATG_G
Cuts at: 984 2981 984
Size: 1997 4161
NgoMIV G'CCGG_C
Cuts at: 4337 4337
Size: 6158
NspI r_CATG'y
Cuts at: 896 1886 2047 3114 3211 3711 5314 896
Size: 990 161 1067 97 500 1603 1740
Fragments arranged by size:
1740 1603 1067 990 500 161 97
PciI A'CATG_T
Cuts at: 2043 5310 2043
Size: 3267 2891
PfoI T'CCnGG_A
Cuts at: 438 1595 438
Size: 1157 5001
PmeI GTTT'AAAC
Cuts at: 3157 3157
Size: 6158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 4604
Size: 32 6126
PpuMI rG'GwC_Cy
Cuts at: 1236 1641 1236
Size: 405 5753
PsiI TTA'TAA
Cuts at: 529 2677 529
Size: 2148 4010
PspOMI G'GGCC_C
Cuts at: 2985 2985
Size: 6158
PstI C_TGCA'G
Cuts at: 951 2670 2707 3205 3890 951
Size: 1719 37 498 685 3219
Fragments arranged by size:
3219 1719 685 498 37
PvuI CG_AT'CG
Cuts at: 104 3129 104
Size: 3025 3133
PvuII CAG'CTG
Cuts at: 247 616 978 2640 3583 3943 247
Size: 369 362 1662 943 360 2462
Fragments arranged by size:
2462 1662 943 369 362 360
RsrII CG'GwC_CG
Cuts at: 4353 4353
Size: 6158
SacI G_AGCT'C
Cuts at: 1481 3172 1481
Size: 1691 4467
SalI G'TCGA_C
Cuts at: 3195 3195
Size: 6158
SapI GCTCTTCn'nnn_
Cuts at: 2360 4181 4391 2360
Size: 1821 210 4127
Fragments arranged by size:
4127 1821 210
SbfI CC_TGCA'GG
Cuts at: 3205 3205
Size: 6158
ScaI AGT'ACT
Cuts at: 2068 2107 5675 2068
Size: 39 3568 2551
Fragments arranged by size:
3568 2551 39
SfcI C'TryA_G
Cuts at: 33 771 947 1636 2666 2703 3010 3201 3886
Size: 738 176 689 1030 37 307 191 685
Cuts at: 3886 4854 5045 33
Size: 968 191 1146
Fragments arranged by size:
1146 1030 968 738 689 685 307 191
191 176 37
SfoI GGC'GCC
Cuts at: 3590 3838 3590
Size: 248 5910
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
SmaI CCC'GGG
Cuts at: 3180 3180
Size: 6158
SmlI C'TyrA_G
Cuts at: 2494 3031 3313 4228 4942 5204 2494
Size: 537 282 915 714 262 3448
Fragments arranged by size:
3448 915 714 537 282 262
SpeI A'CTAG_T
Cuts at: 67 1567 67
Size: 1500 4658
SphI G_CATG'C
Cuts at: 3114 3211 3711 3114
Size: 97 500 5561
Fragments arranged by size:
5561 500 97
SspI AAT'ATT
Cuts at: 1814 2781 1814
Size: 967 5191
StuI AGG'CCT
Cuts at: 869 2469 2604 2770 869
Size: 1600 135 166 4257
Fragments arranged by size:
4257 1600 166 135
StyI C'CwwG_G
Cuts at: 984 1903 2981 3292 3646 984
Size: 919 1078 311 354 3496
Fragments arranged by size:
3496 1078 919 354 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3741 3874 4333 4542 3741
Size: 133 459 209 5357
Fragments arranged by size:
5357 459 209 133
TatI w'GTAC_w
Cuts at: 303 532 2066 2105 5673 303
Size: 229 1534 39 3568 788
Fragments arranged by size:
3568 1534 788 229 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2605 2637 2910 2942 5675 5707 2605
Size: 32 273 32 2733 32 3056
Fragments arranged by size:
3056 2733 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3955 3955
Size: 6158
XbaI T'CTAG_A
Cuts at: 3189 3189
Size: 6158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2043 2996 3653 2043
Size: 953 657 4548
Fragments arranged by size:
4548 953 657
XmaI C'CCGG_G
Cuts at: 3178 3178
Size: 6158
XmnI GAAnn'nnTTC
Cuts at: 447 1325 447
Size: 878 5280
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BspEI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI