Restriction map for
pF1KA0972
(Circular) (Six-base) MAPSORT of: pF1KA0972.seq Check: 7221 from: 1 to: 5162
pF1KA0972 5162 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1040 1040
Size: 5162
AatII G_ACGT'C
Cuts at: 1273 1273
Size: 5162
AccI GT'mk_AC
Cuts at: 2110 2200 2110
Size: 90 5072
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2987 3419 3772 4434 2987
Size: 432 353 662 3715
Fragments arranged by size:
3715 662 432 353
AflIII A'CryG_T
Cuts at: 2514 4314 4595 2514
Size: 1800 281 3081
Fragments arranged by size:
3081 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 617 617
Size: 5162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 406 438 406
Size: 32 5130
AlwNI CAG_nnn'CTG
Cuts at: 2467 3905 2467
Size: 1438 3724
ApaLI G'TGCA_C
Cuts at: 4000 4000
Size: 5162
ApoI r'AATT_y
Cuts at: 268 436 651 670 732 778 964 1463 1547
Size: 168 215 19 62 46 186 499 84
Cuts at: 1547 1631 1883 1930 1967 2132 2166 4515 4771
Size: 84 252 47 37 165 34 2349 256
Cuts at: 4771 268
Size: 659
Fragments arranged by size:
2349 659 499 256 252 215 186 168
165 84 84 62 47 46 37 34
19
AseI AT'TA_AT
Cuts at: 20 680 20
Size: 660 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 2182 2182
Size: 5162
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 357 390 357
Size: 33 5129
BamHI G'GATC_C
Cuts at: 2187 2187
Size: 5162
BanI G'GyrC_C
Cuts at: 2178 2592 2840 2875 2178
Size: 414 248 35 4465
Fragments arranged by size:
4465 414 248 35
BanII G_rGCy'C
Cuts at: 2176 3206 2176
Size: 1030 4132
BbeI G_GCGC'C
Cuts at: 2596 2844 2596
Size: 248 4914
BciVI GTATCCnnnnn_n'
Cuts at: 3060 4116 5162 3060
Size: 1056 1046 3060
Fragments arranged by size:
3060 1056 1046
BclI T'GATC_A
Cuts at: 482 1956 2682 482
Size: 1474 726 2962
Fragments arranged by size:
2962 1474 726
BglII A'GATC_T
Cuts at: 2677 4714 2677
Size: 2037 3125
BlpI GC'TnA_GC
Cuts at: 2274 2274
Size: 5162
Bme1580I G_kGCm'C
Cuts at: 2787 2880 4004 2787
Size: 93 1124 3945
Fragments arranged by size:
3945 1124 93
BmgBI CAC'GTC
Cuts at: 258 258
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 1733 1901 2374 2455 2790 4934 1733
Size: 168 473 81 335 2144 1961
Fragments arranged by size:
2144 1961 473 335 168 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 3529 456
Size: 3073 2089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 791 355
Size: 436 4726
Bpu10I CC'TnA_GC
Cuts at: 2116 2116
Size: 5162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2338 3217 3931 4229 2338
Size: 879 714 298 3271
Fragments arranged by size:
3271 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4866 4866
Size: 5162
BsaAI yAC'GTr
Cuts at: 2515 2515
Size: 5162
BsaBI GATnn'nnATC
Cuts at: 6 2676 4713 6
Size: 2670 2037 455
Fragments arranged by size:
2670 2037 455
BsaHI Gr'CG_yC
Cuts at: 1270 2593 2841 3543 5039 1270
Size: 1323 248 702 1496 1393
Fragments arranged by size:
1496 1393 1323 702 248
BsaWI w'CCGG_w
Cuts at: 1937 2351 2872 3550 3961 4108 4382 1937
Size: 414 521 678 411 147 274 2717
Fragments arranged by size:
2717 678 521 414 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 406 436 406
Size: 30 5132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 427 445 295
Size: 132 18 5012
Fragments arranged by size:
5012 132 18
BseYI C'CCAG_C
Cuts at: 2587 3345 4010 4418 2587
Size: 758 665 408 3331
Fragments arranged by size:
3331 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2704 2750 3980 104
Size: 2600 46 1230 1286
Fragments arranged by size:
2600 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2176 2954 3144 4004 2176
Size: 778 190 860 3334
Fragments arranged by size:
3334 860 778 190
BsmI GAATG_Cn'
Cuts at: 2083 2083
Size: 5162
BsmBI CGTCTCn'nnnn_
Cuts at: 360 360
Size: 5162
Bsp1286I G_dGCh'C
Cuts at: 2176 2787 2880 2954 3144 3206 4004 2176
Size: 611 93 74 190 62 798 3334
Fragments arranged by size:
3334 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1937 1937
Size: 5162
BspHI T'CATG_A
Cuts at: 1245 3594 1245
Size: 2349 2813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1040 2212 2426 2728 3109 1040
Size: 1172 214 302 381 3093
Fragments arranged by size:
3093 1172 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3454 3508 4992 5157 50
Size: 3404 54 1484 165 55
Fragments arranged by size:
3404 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2088 3074 2088
Size: 986 4176
BsrFI r'CCGG_y
Cuts at: 3160 3341 3160
Size: 181 4981
BsrGI T'GTAC_A
Cuts at: 317 317
Size: 5162
BssSI C'ACGA_G
Cuts at: 3433 4141 3433
Size: 708 4454
BstAPI GCAn_nnn'nTGC
Cuts at: 2539 2539
Size: 5162
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstYI r'GATC_y
Cuts at: 1 2187 2654 2677 3012 3258 3564 3576 3662
Size: 2186 467 23 335 246 306 12 86
Cuts at: 3662 4714 1
Size: 1052 449
Fragments arranged by size:
2186 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1531 1531
Size: 5162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3112 3293 3456 3112
Size: 181 163 4818
Fragments arranged by size:
4818 181 163
BtsI GCAGTG_nn'
Cuts at: 2405 2405
Size: 5162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1650 1685 1650
Size: 35 5127
DraI TTT'AAA
Cuts at: 1908 2161 1908
Size: 253 4909
DrdI GACnn_nn'nnGTC
Cuts at: 2868 4212 2868
Size: 1344 3818
EaeI y'GGCC_r
Cuts at: 1683 2019 2747 2921 3312 3339 4684 1683
Size: 336 728 174 391 27 1345 2161
Fragments arranged by size:
2161 1345 728 391 336 174 27
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 557 1181 3185 3395 557
Size: 624 2004 210 2324
Fragments arranged by size:
2324 2004 624 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 4112 4258 4972 3370
Size: 742 146 714 3560
Fragments arranged by size:
3560 742 714 146
EcoICRI GAG'CTC
Cuts at: 2174 2174
Size: 5162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 791 2987 3419 3772 4434 355
Size: 436 2196 432 353 662 1083
Fragments arranged by size:
2196 1083 662 436 432 353
EcoO109I rG'GnC_Cy
Cuts at: 2301 2301
Size: 5162
EcoRI G'AATT_C
Cuts at: 268 436 732 1463 1547 1631 1883 1967 2166
Size: 168 296 731 84 84 252 84 199
Cuts at: 2166 268
Size: 3264
Fragments arranged by size:
3264 731 296 252 199 168 84 84
84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1966 3181 1966
Size: 1215 3947
FspI TGC'GCA
Cuts at: 2943 2943
Size: 5162
HaeII r_GCGC'y
Cuts at: 2596 2844 4074 4845 2596
Size: 248 1230 771 2913
Fragments arranged by size:
2913 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3004 3034 3036 3066 3004
Size: 30 2 30 5100
Fragments arranged by size:
5100 30 30 2
HincII GTy'rAC
Cuts at: 1070 1526 2201 1070
Size: 456 675 4031
Fragments arranged by size:
4031 675 456
HindIII A'AGCT_T
Cuts at: 863 2118 863
Size: 1255 3907
KasI G'GCGC_C
Cuts at: 2592 2840 2592
Size: 248 4914
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 3921 4105 380
Size: 3541 184 1437
Fragments arranged by size:
3541 1437 184
MscI TGG'CCA
Cuts at: 1685 2021 2923 1685
Size: 336 902 3924
Fragments arranged by size:
3924 902 336
MslI CAynn'nnrTG
Cuts at: 1404 1824 1908 3151 3278 1404
Size: 420 84 1243 127 3288
Fragments arranged by size:
3288 1243 420 127 84
MspA1I CmG'CkG
Cuts at: 1177 2273 2587 2947 3731 3976 1177
Size: 1096 314 360 784 245 2363
Fragments arranged by size:
2363 1096 784 360 314 245
NaeI GCC'GGC
Cuts at: 3343 3343
Size: 5162
NarI GG'CG_CC
Cuts at: 2593 2841 2593
Size: 248 4914
NgoMIV G'CCGG_C
Cuts at: 3341 3341
Size: 5162
NspI r_CATG'y
Cuts at: 598 2215 2715 4318 598
Size: 1617 500 1603 1442
Fragments arranged by size:
1617 1603 1442 500
PciI A'CATG_T
Cuts at: 4314 4314
Size: 5162
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 406 438 3608 3640 406
Size: 32 3170 32 1928
Fragments arranged by size:
3170 1928 32 32
PsiI TTA'TAA
Cuts at: 818 818
Size: 5162
PstI C_TGCA'G
Cuts at: 2209 2894 2209
Size: 685 4477
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 1177 2587 2947 1177
Size: 1410 360 3392
Fragments arranged by size:
3392 1410 360
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 2176 2176
Size: 5162
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 3185 3395 3185
Size: 210 4952
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 1452 4679 1452
Size: 3227 1935
SfcI C'TryA_G
Cuts at: 33 263 342 554 2205 2890 3858 4049 33
Size: 230 79 212 1651 685 968 191 1146
Fragments arranged by size:
1651 1146 968 685 230 212 191 79
SfoI GGC'GCC
Cuts at: 2594 2842 2594
Size: 248 4914
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 2184 2184
Size: 5162
SmlI C'TyrA_G
Cuts at: 2317 3232 3946 4208 2317
Size: 915 714 262 3271
Fragments arranged by size:
3271 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 2215 2715 2215
Size: 500 4662
StyI C'CwwG_G
Cuts at: 418 2296 2650 418
Size: 1878 354 2930
Fragments arranged by size:
2930 1878 354
SwaI ATTT'AAAT
Cuts at: 1908 1908
Size: 5162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 470 2745 2878 3337 3546 289
Size: 181 2275 133 459 209 1905
Fragments arranged by size:
2275 1905 459 209 181 133
TatI w'GTAC_w
Cuts at: 317 1450 4677 317
Size: 1133 3227 802
Fragments arranged by size:
3227 1133 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 963 995 2043 2075 4679 4711 963
Size: 32 1048 32 2604 32 1414
Fragments arranged by size:
2604 1414 1048 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2959 2959
Size: 5162
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1009 2657 1009
Size: 1648 3514
XmaI C'CCGG_G
Cuts at: 2182 2182
Size: 5162
XmnI GAAnn'nnTTC
Cuts at: 693 827 1342 1819 693
Size: 134 515 477 4036
Fragments arranged by size:
4036 515 477 134
ZraI GAC'GTC
Cuts at: 1271 1271
Size: 5162
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI
BssHII BstEII BstXI BstZ17I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI XhoI