Restriction map for
pF1KA0970
(Circular) (Six-base) MAPSORT of: pF1KA0970.seq Check: 4831 from: 1 to: 6539
pF1KA0970 6539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1956 3577 1956
Size: 1621 4918
Acc65I G'GTAC_C
Cuts at: 3555 3555
Size: 6539
AclI AA'CG_TT
Cuts at: 492 6379 492
Size: 5887 652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1451 1634 2108 2121 2887 3247 4364 4796
Size: 453 183 474 13 766 360 1117 432
Cuts at: 4796 5543 5811 998
Size: 747 268 1726
Fragments arranged by size:
1726 1117 766 747 474 453 432 360
268 183 13
AflII C'TTAA_G
Cuts at: 2839 2839
Size: 6539
AflIII A'CryG_T
Cuts at: 2242 3891 4995 5972 2242
Size: 1649 1104 977 2809
Fragments arranged by size:
2809 1649 1104 977
AgeI A'CCGG_T
Cuts at: 4927 4927
Size: 6539
AleI CACnn'nnGTG
Cuts at: 3125 3358 3125
Size: 233 6306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3376 3476 3376
Size: 100 6439
AlwNI CAG_nnn'CTG
Cuts at: 1021 2206 2433 2497 3844 5411 1021
Size: 1185 227 64 1347 1567 2149
Fragments arranged by size:
2149 1567 1347 1185 227 64
ApaLI G'TGCA_C
Cuts at: 2261 5309 2261
Size: 3048 3491
ApoI r'AATT_y
Cuts at: 1092 1186 1258 1433 1549 1606 2720 2998 3211
Size: 94 72 175 116 57 1114 278 213
Cuts at: 3211 3543 5892 6148 1092
Size: 332 2349 256 1483
Fragments arranged by size:
2349 1483 1114 332 278 256 213 175
116 94 72 57
AseI AT'TA_AT
Cuts at: 20 584 818 20
Size: 564 234 5741
Fragments arranged by size:
5741 564 234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
AvaI C'yCGr_G
Cuts at: 3559 3559
Size: 6539
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 665 698 665
Size: 33 6506
BamHI G'GATC_C
Cuts at: 1695 3564 1695
Size: 1869 4670
BanI G'GyrC_C
Cuts at: 237 1116 3555 3969 4217 4252 237
Size: 879 2439 414 248 35 2524
Fragments arranged by size:
2524 2439 879 414 248 35
BanII G_rGCy'C
Cuts at: 854 1210 3553 4583 854
Size: 356 2343 1030 2810
Fragments arranged by size:
2810 2343 1030 356
BbeI G_GCGC'C
Cuts at: 3973 4221 3973
Size: 248 6291
BbsI GAAGACnn'nnnn_
Cuts at: 206 398 2506 3175 206
Size: 192 2108 669 3570
Fragments arranged by size:
3570 2108 669 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1838 1872 3439 3473 1838
Size: 34 1567 34 4904
Fragments arranged by size:
4904 1567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2943 4437 5198 6539 2943
Size: 1494 761 1341 2943
Fragments arranged by size:
2943 1494 1341 761
BclI T'GATC_A
Cuts at: 2598 3117 4059 2598
Size: 519 942 5078
Fragments arranged by size:
5078 942 519
BglII A'GATC_T
Cuts at: 1503 2248 4054 6091 1503
Size: 745 1806 2037 1951
Fragments arranged by size:
2037 1951 1806 745
BlpI GC'TnA_GC
Cuts at: 3651 3651
Size: 6539
Bme1580I G_kGCm'C
Cuts at: 2265 3441 4164 4257 5313 2265
Size: 1176 723 93 1056 3491
Fragments arranged by size:
3491 1176 1056 723 93
BmgBI CAC'GTC
Cuts at: 340 340
Size: 6539
BmrI ACTGGGnnnn_n'
Cuts at: 3751 3832 4167 6311 3751
Size: 81 335 2144 3979
Fragments arranged by size:
3979 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4906 4906
Size: 6539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 389 1895 2341 383
Size: 6 1506 446 4581
Fragments arranged by size:
4581 1506 446 6
Bpu10I CC'TnA_GC
Cuts at: 122 122
Size: 6539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 861 934 1059 2219 3715 4594 5086 5384 861
Size: 73 125 1160 1496 879 492 298 2016
Fragments arranged by size:
2016 1496 1160 879 492 298 125 73
BsaI GGTCTCn'nnnn_
Cuts at: 1890 2377 6243 1890
Size: 487 3866 2186
Fragments arranged by size:
3866 2186 487
BsaAI yAC'GTr
Cuts at: 2146 3892 2146
Size: 1746 4793
BsaBI GATnn'nnATC
Cuts at: 6 4053 6090 6
Size: 4047 2037 455
Fragments arranged by size:
4047 2037 455
BsaHI Gr'CG_yC
Cuts at: 3970 4218 4920 6416 3970
Size: 248 702 1496 4093
Fragments arranged by size:
4093 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3248 3728 4249 4927 5201 5348 5759 3248
Size: 480 521 678 274 147 411 4028
Fragments arranged by size:
4028 678 521 480 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 281 2345 281
Size: 2064 4475
BseYI C'CCAG_C
Cuts at: 2582 3025 3078 3964 4722 5299 5795 2582
Size: 443 53 886 758 577 496 3326
Fragments arranged by size:
3326 886 758 577 496 443 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2168 2468 1924
Size: 244 300 5995
Fragments arranged by size:
5995 300 244
BsiEI CG_ry'CG
Cuts at: 104 4081 4127 5335 104
Size: 3977 46 1208 1308
Fragments arranged by size:
3977 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 854 1338 2265 3365 3553 4331 4521 5313 854
Size: 484 927 1100 188 778 190 792 2080
Fragments arranged by size:
2080 1100 927 792 778 484 190 188
Bsp1286I G_dGCh'C
Cuts at: 854 1210 1338 2265 3365 3441 3553 4164 4257
Size: 356 128 927 1100 76 112 611 93
Cuts at: 4257 4331 4521 4583 5313 854
Size: 74 190 62 730 2080
Fragments arranged by size:
2080 1100 927 730 611 356 190 128
112 93 76 74 62
BspEI T'CCGG_A
Cuts at: 5759 5759
Size: 6539
BspHI T'CATG_A
Cuts at: 5715 5715
Size: 6539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3589 3803 4105 4486 3589
Size: 214 302 381 5642
Fragments arranged by size:
5642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4831 4885 6369 6534 50
Size: 4781 54 1484 165 55
Fragments arranged by size:
4781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1114 1253 4451 1114
Size: 139 3198 3202
Fragments arranged by size:
3202 3198 139
BsrFI r'CCGG_y
Cuts at: 4537 4718 4927 4537
Size: 181 209 6149
Fragments arranged by size:
6149 209 181
BsrGI T'GTAC_A
Cuts at: 2098 2098
Size: 6539
BssSI C'ACGA_G
Cuts at: 4810 5168 4810
Size: 358 6181
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 3916 2260
Size: 1656 4883
BstBI TT'CG_AA
Cuts at: 213 4900 213
Size: 4687 1852
BstEII G'GTnAC_C
Cuts at: 797 797
Size: 6539
BstYI r'GATC_y
Cuts at: 1 1503 1695 2248 3564 4031 4054 4389 4635
Size: 1502 192 553 1316 467 23 335 246
Cuts at: 4635 5647 5733 5745 6091 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1502 1316 1012 553 467 449 346 335
246 192 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 233 1326 1410 233
Size: 1093 84 5362
Fragments arranged by size:
5362 1093 84
BtgI C'CryG_G
Cuts at: 959 959
Size: 6539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2543 4489 4670 4833 2543
Size: 1946 181 163 4249
Fragments arranged by size:
4249 1946 181 163
BtsI GCAGTG_nn'
Cuts at: 3460 3782 3460
Size: 322 6217
DraI TTT'AAA
Cuts at: 941 1224 1683 3538 941
Size: 283 459 1855 3942
Fragments arranged by size:
3942 1855 459 283
DraIII CAC_nnn'GTG
Cuts at: 1123 3445 1123
Size: 2322 4217
DrdI GACnn_nn'nnGTC
Cuts at: 4245 5103 4245
Size: 858 5681
EaeI y'GGCC_r
Cuts at: 1271 4124 4298 4689 4716 6061 1271
Size: 2853 174 391 27 1345 1749
Fragments arranged by size:
2853 1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4124 4124
Size: 6539
EarI CTCTTCn'nnn_
Cuts at: 210 3246 3370 4562 4772 210
Size: 3036 124 1192 210 1977
Fragments arranged by size:
3036 1977 1192 210 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1730 4747 5057 5203 6349 1730
Size: 3017 310 146 1146 1920
Fragments arranged by size:
3017 1920 1146 310 146
EcoICRI GAG'CTC
Cuts at: 852 3551 852
Size: 2699 3840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 389 998 1451 1634 1895 2108 2121 2341
Size: 6 609 453 183 261 213 13 220
Cuts at: 2341 2887 3247 4364 4796 5543 5811 383
Size: 546 360 1117 432 747 268 1111
Fragments arranged by size:
1117 1111 747 609 546 453 432 360
268 261 220 213 183 13 6
EcoO109I rG'GnC_Cy
Cuts at: 1990 2452 3034 3232 3333 3343 3428 3678 1990
Size: 462 582 198 101 10 85 250 4851
Fragments arranged by size:
4851 582 462 250 198 101 85 10
EcoRI G'AATT_C
Cuts at: 2720 3211 3543 2720
Size: 491 332 5716
Fragments arranged by size:
5716 491 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 751 4558 751
Size: 3807 2732
FspI TGC'GCA
Cuts at: 4320 4320
Size: 6539
HaeII r_GCGC'y
Cuts at: 3973 4221 5243 6222 3973
Size: 248 1022 979 4290
Fragments arranged by size:
4290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 315 347 421 453 457 489 1065 1097 1709
Size: 32 74 32 4 32 576 32 612
Cuts at: 1709 1741 1996 2028 2529 2561 2632 2664 2974
Size: 32 255 32 501 32 71 32 310
Cuts at: 2974 3006 4381 4411 4413 4443 315
Size: 32 1375 30 2 30 2411
Fragments arranged by size:
2411 1375 612 576 501 310 255 74
71 32 32 32 32 32 32 32
32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 2879 2987 3578 2879
Size: 108 591 5840
Fragments arranged by size:
5840 591 108
HindIII A'AGCT_T
Cuts at: 3011 3011
Size: 6539
KasI G'GCGC_C
Cuts at: 3969 4217 3969
Size: 248 6291
KpnI G_GTAC'C
Cuts at: 3559 3559
Size: 6539
MluI A'CGCG_T
Cuts at: 5972 5972
Size: 6539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 764 788 5210 5394 479
Size: 285 24 4422 184 1624
Fragments arranged by size:
4422 1624 285 184 24
MscI TGG'CCA
Cuts at: 4300 4300
Size: 6539
MslI CAynn'nnrTG
Cuts at: 1811 3125 3141 3358 3472 4528 4655 1811
Size: 1314 16 217 114 1056 127 3695
Fragments arranged by size:
3695 1314 1056 217 127 114 16
MspA1I CmG'CkG
Cuts at: 1915 3082 3267 3650 3964 4324 5337 5582 1915
Size: 1167 185 383 314 360 1013 245 2872
Fragments arranged by size:
2872 1167 1013 383 360 314 245 185
NaeI GCC'GGC
Cuts at: 4720 4720
Size: 6539
NarI GG'CG_CC
Cuts at: 3970 4218 3970
Size: 248 6291
NcoI C'CATG_G
Cuts at: 959 959
Size: 6539
NdeI CA'TA_TG
Cuts at: 344 486 2762 344
Size: 142 2276 4121
Fragments arranged by size:
4121 2276 142
NgoMIV G'CCGG_C
Cuts at: 4718 4718
Size: 6539
NspI r_CATG'y
Cuts at: 2246 2570 3592 4092 4999 2246
Size: 324 1022 500 907 3786
Fragments arranged by size:
3786 1022 907 500 324
PasI CC'CwG_GG
Cuts at: 2206 2206
Size: 6539
PciI A'CATG_T
Cuts at: 2242 4995 2242
Size: 2753 3786
PfoI T'CCnGG_A
Cuts at: 174 2118 174
Size: 1944 4595
PmeI GTTT'AAAC
Cuts at: 3538 3538
Size: 6539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5678 5710 5678
Size: 32 6507
PpuMI rG'GwC_Cy
Cuts at: 1990 3034 3232 3333 3343 3428 1990
Size: 1044 198 101 10 85 5101
Fragments arranged by size:
5101 1044 198 101 85 10
PsiI TTA'TAA
Cuts at: 604 604
Size: 6539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1040 1072 1040
Size: 32 6507
PstI C_TGCA'G
Cuts at: 3586 4271 3586
Size: 685 5854
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6539
PvuII CAG'CTG
Cuts at: 1915 3082 3267 3964 4324 1915
Size: 1167 185 697 360 4130
Fragments arranged by size:
4130 1167 697 360 185
RsrII CG'GwC_CG
Cuts at: 4734 4734
Size: 6539
SacI G_AGCT'C
Cuts at: 854 3553 854
Size: 2699 3840
SalI G'TCGA_C
Cuts at: 3576 3576
Size: 6539
SanDI GG'GwC_CC
Cuts at: 1990 3232 1990
Size: 1242 5297
SapI GCTCTTCn'nnn_
Cuts at: 3370 4562 4772 3370
Size: 1192 210 5137
Fragments arranged by size:
5137 1192 210
SbfI CC_TGCA'GG
Cuts at: 3586 3586
Size: 6539
ScaI AGT'ACT
Cuts at: 788 882 1539 3520 6056 788
Size: 94 657 1981 2536 1271
Fragments arranged by size:
2536 1981 1271 657 94
SexAI A'CCwGG_T
Cuts at: 297 793 297
Size: 496 6043
SfcI C'TryA_G
Cuts at: 33 2048 2198 2622 3348 3582 4267 5260 5451
Size: 2015 150 424 726 234 685 993 191
Cuts at: 5451 33
Size: 1121
Fragments arranged by size:
2015 1121 993 726 685 424 234 191
150
SfoI GGC'GCC
Cuts at: 3971 4219 3971
Size: 248 6291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
SmaI CCC'GGG
Cuts at: 3561 3561
Size: 6539
SmlI C'TyrA_G
Cuts at: 876 949 1038 2234 2839 3694 4609 5101 5363
Size: 73 89 1196 605 855 915 492 262
Cuts at: 5363 876
Size: 2052
Fragments arranged by size:
2052 1196 915 855 605 492 262 89
73
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6539
SphI G_CATG'C
Cuts at: 3592 4092 3592
Size: 500 6039
StyI C'CwwG_G
Cuts at: 519 959 1069 1367 2079 2739 3673 4027 519
Size: 440 110 298 712 660 934 354 3031
Fragments arranged by size:
3031 934 712 660 440 354 298 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2434 4122 4255 4714 4923 2031
Size: 403 1688 133 459 209 3647
Fragments arranged by size:
3647 1688 459 403 209 133
TatI w'GTAC_w
Cuts at: 347 786 880 1537 2098 2967 3518 6054 347
Size: 439 94 657 561 869 551 2536 832
Fragments arranged by size:
2536 869 832 657 561 551 439 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6056 6088 6056
Size: 32 6507
Tth111I GACn'n_nGTC
Cuts at: 2874 2951 4336 2874
Size: 77 1385 5077
Fragments arranged by size:
5077 1385 77
XbaI T'CTAG_A
Cuts at: 3570 3570
Size: 6539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4034 4034
Size: 6539
XmaI C'CCGG_G
Cuts at: 3559 3559
Size: 6539
XmnI GAAnn'nnTTC
Cuts at: 731 1011 3204 731
Size: 280 2193 4066
Fragments arranged by size:
4066 2193 280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BbvCI BfrBI BglI BmtI BsaXI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PspOMI PspXI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI