Restriction map for
pF1KA0868
(Circular) (Six-base) MAPSORT of: pF1KA0868.seq Check: 3827 from: 1 to: 7106
pF1KA0868 7106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1882 2408 1882
Size: 526 6580
AatII G_ACGT'C
Cuts at: 763 763
Size: 7106
AccI GT'mk_AC
Cuts at: 3206 3413 4144 3206
Size: 207 731 6168
Fragments arranged by size:
6168 731 207
Acc65I G'GTAC_C
Cuts at: 4122 4122
Size: 7106
AclI AA'CG_TT
Cuts at: 6946 6946
Size: 7106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1326 1630 2677 3249 3255 4931 5363 5716
Size: 980 304 1047 572 6 1676 432 353
Cuts at: 5716 6378 346
Size: 662 1074
Fragments arranged by size:
1676 1074 1047 980 662 572 432 353
304 6
AfeI AGC'GCT
Cuts at: 143 2568 143
Size: 2425 4681
AflIII A'CryG_T
Cuts at: 1757 3962 4458 6258 6539 1757
Size: 2205 496 1800 281 2324
Fragments arranged by size:
2324 2205 1800 496 281
AleI CACnn'nnGTG
Cuts at: 898 2671 898
Size: 1773 5333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 7106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1548 1580 1548
Size: 32 7074
AlwNI CAG_nnn'CTG
Cuts at: 167 182 1287 1459 4411 5849 167
Size: 15 1105 172 2952 1438 1424
Fragments arranged by size:
2952 1438 1424 1105 172 15
ApaLI G'TGCA_C
Cuts at: 3936 5944 3936
Size: 2008 5098
ApoI r'AATT_y
Cuts at: 1101 1194 1509 1701 4110 6459 6715 1101
Size: 93 315 192 2409 2349 256 1492
Fragments arranged by size:
2409 2349 1492 315 256 192 93
AseI AT'TA_AT
Cuts at: 20 20
Size: 7106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
AvaI C'yCGr_G
Cuts at: 2997 3389 4126 2997
Size: 392 737 5977
Fragments arranged by size:
5977 737 392
AvrII C'CTAG_G
Cuts at: 1920 1920
Size: 7106
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 990 1023 3048 3081 990
Size: 33 2025 33 5015
Fragments arranged by size:
5015 2025 33 33
BamHI G'GATC_C
Cuts at: 4131 4131
Size: 7106
BanI G'GyrC_C
Cuts at: 1227 1263 2753 3168 3285 3385 3800 3979 4122
Size: 36 1490 415 117 100 415 179 143
Cuts at: 4122 4536 4784 4819 1227
Size: 414 248 35 3514
Fragments arranged by size:
3514 1490 415 415 414 248 179 143
117 100 36 35
BanII G_rGCy'C
Cuts at: 1181 2861 4120 5150 1181
Size: 1680 1259 1030 3137
Fragments arranged by size:
3137 1680 1259 1030
BbeI G_GCGC'C
Cuts at: 3289 4540 4788 3289
Size: 1251 248 5607
Fragments arranged by size:
5607 1251 248
BbsI GAAGACnn'nnnn_
Cuts at: 1332 3478 3939 1332
Size: 2146 461 4499
Fragments arranged by size:
4499 2146 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1471 1505 90
Size: 34 1347 34 5691
Fragments arranged by size:
5691 1347 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1852 5004 6060 7106 1852
Size: 3152 1056 1046 1852
Fragments arranged by size:
3152 1852 1056 1046
BclI T'GATC_A
Cuts at: 4626 4626
Size: 7106
BfrBI ATG'CAT
Cuts at: 1667 1667
Size: 7106
BglI GCCn_nnn'nGGC
Cuts at: 112 187 1272 2900 3977 112
Size: 75 1085 1628 1077 3241
Fragments arranged by size:
3241 1628 1085 1077 75
BglII A'GATC_T
Cuts at: 4621 6658 4621
Size: 2037 5069
BlpI GC'TnA_GC
Cuts at: 4218 4218
Size: 7106
Bme1580I G_kGCm'C
Cuts at: 1268 1787 3940 3982 4731 4824 5948 1268
Size: 519 2153 42 749 93 1124 2426
Fragments arranged by size:
2426 2153 1124 749 519 93 42
BmgBI CAC'GTC
Cuts at: 200 3714 200
Size: 3514 3592
BmrI ACTGGGnnnn_n'
Cuts at: 666 2268 4318 4399 4734 6878 666
Size: 1602 2050 81 335 2144 894
Fragments arranged by size:
2144 2050 1602 894 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5473 5473
Size: 7106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1071 1279 2671 2875 2974 3177 3630 1071
Size: 208 1392 204 99 203 453 4547
Fragments arranged by size:
4547 1392 453 208 204 203 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1614 2946 3469 3661 3708 4282 5161 5875 6173
Size: 1332 523 192 47 574 879 714 298
Cuts at: 6173 1614
Size: 2547
Fragments arranged by size:
2547 1332 879 714 574 523 298 192
47
BsaI GGTCTCn'nnnn_
Cuts at: 335 1336 4060 6810 335
Size: 1001 2724 2750 631
Fragments arranged by size:
2750 2724 1001 631
BsaAI yAC'GTr
Cuts at: 4459 4459
Size: 7106
BsaBI GATnn'nnATC
Cuts at: 6 3497 4620 6657 6
Size: 3491 1123 2037 455
Fragments arranged by size:
3491 2037 1123 455
BsaHI Gr'CG_yC
Cuts at: 760 1139 2050 3286 3905 4031 4537 4785 5487
Size: 379 911 1236 619 126 506 248 702
Cuts at: 5487 6983 760
Size: 1496 883
Fragments arranged by size:
1496 1236 911 883 702 619 506 379
248 126
BsaWI w'CCGG_w
Cuts at: 446 2989 3956 4295 4816 5494 5905 6052 6326
Size: 2543 967 339 521 678 411 147 274
Cuts at: 6326 446
Size: 1226
Fragments arranged by size:
2543 1226 967 678 521 411 339 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2092 2122 2512 2542 2644 2674 3052 3082 3295
Size: 30 390 30 102 30 378 30 213
Cuts at: 3295 3325 2092
Size: 30 5873
Fragments arranged by size:
5873 390 378 213 102 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 252 326 3268 3289 249
Size: 3 74 2942 21 4066
Fragments arranged by size:
4066 2942 74 21 3
BseYI C'CCAG_C
Cuts at: 319 1081 2887 4531 5289 5954 6362 319
Size: 762 1806 1644 758 665 408 1063
Fragments arranged by size:
1806 1644 1063 762 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 2298 2521 769
Size: 1529 223 5354
Fragments arranged by size:
5354 1529 223
BsiEI CG_ry'CG
Cuts at: 104 4648 4694 5924 104
Size: 4544 46 1230 1286
Fragments arranged by size:
4544 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1816 2861 3940 4120 4898 5088 5948 1816
Size: 1045 1079 180 778 190 860 2974
Fragments arranged by size:
2974 1079 1045 860 778 190 180
BsmI GAATG_Cn'
Cuts at: 936 2508 3293 936
Size: 1572 785 4749
Fragments arranged by size:
4749 1572 785
Bsp1286I G_dGCh'C
Cuts at: 1181 1268 1787 1816 2861 3940 3982 4120 4731
Size: 87 519 29 1045 1079 42 138 611
Cuts at: 4731 4824 4898 5088 5150 5948 1181
Size: 93 74 190 62 798 2339
Fragments arranged by size:
2339 1079 1045 798 611 519 190 138
93 87 74 62 42 29
BspEI T'CCGG_A
Cuts at: 2989 2989
Size: 7106
BspHI T'CATG_A
Cuts at: 2031 4040 5538 2031
Size: 2009 1498 3599
Fragments arranged by size:
3599 2009 1498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1882 2408 2543 4156 4370 4672 5053 1882
Size: 526 135 1613 214 302 381 3935
Fragments arranged by size:
3935 1613 526 381 302 214 135
BsrBI CCG'CTC
Cuts at: 50 2921 5398 5452 6936 7101 50
Size: 2871 2477 54 1484 165 55
Fragments arranged by size:
2871 2477 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 398 740 1374 2365 3003 3908 5018 398
Size: 342 634 991 638 905 1110 2486
Fragments arranged by size:
2486 1110 991 905 638 634 342
BsrFI r'CCGG_y
Cuts at: 127 2804 5104 5285 127
Size: 2677 2300 181 1948
Fragments arranged by size:
2677 2300 1948 181
BssHII G'CGCG_C
Cuts at: 123 123
Size: 7106
BssSI C'ACGA_G
Cuts at: 1483 3466 5377 6085 1483
Size: 1983 1911 708 2504
Fragments arranged by size:
2504 1983 1911 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3181 4483 3181
Size: 1302 5804
BstBI TT'CG_AA
Cuts at: 1554 5467 1554
Size: 3913 3193
BstEII G'GTnAC_C
Cuts at: 435 2723 435
Size: 2288 4818
BstYI r'GATC_y
Cuts at: 1 1950 2319 2819 3257 4131 4598 4621 4956
Size: 1949 369 500 438 874 467 23 335
Cuts at: 4956 5202 5508 5520 5606 6658 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1949 1052 874 500 467 449 438 369
335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1452 1452
Size: 7106
BtgI C'CryG_G
Cuts at: 2111 3060 2111
Size: 949 6157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5056 5237 5400 5056
Size: 181 163 6762
Fragments arranged by size:
6762 181 163
BtsI GCAGTG_nn'
Cuts at: 2762 4349 2762
Size: 1587 5519
ClaI AT'CG_AT
Cuts at: 1442 1529 1442
Size: 87 7019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3363 3398 3363
Size: 35 7071
DraI TTT'AAA
Cuts at: 4105 4105
Size: 7106
DraIII CAC_nnn'GTG
Cuts at: 2225 2225
Size: 7106
DrdI GACnn_nn'nnGTC
Cuts at: 541 2025 4812 6156 541
Size: 1484 2787 1344 1491
Fragments arranged by size:
2787 1491 1484 1344
EaeI y'GGCC_r
Cuts at: 681 888 3428 4691 4865 5256 5283 6628 681
Size: 207 2540 1263 174 391 27 1345 1159
Fragments arranged by size:
2540 1345 1263 1159 391 207 174 27
EagI C'GGCC_G
Cuts at: 4691 4691
Size: 7106
EarI CTCTTCn'nnn_
Cuts at: 3547 5129 5339 3547
Size: 1582 210 5314
Fragments arranged by size:
5314 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 978 3774 3958 4024 5314 6056 6202 6916 978
Size: 2796 184 66 1290 742 146 714 1168
Fragments arranged by size:
2796 1290 1168 742 714 184 146 66
EcoICRI GAG'CTC
Cuts at: 2859 4118 2859
Size: 1259 5847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1071 1279 1326 1630 2671 2677 2875 2974
Size: 725 208 47 304 1041 6 198 99
Cuts at: 2974 3177 3249 3255 3630 4931 5363 5716 6378
Size: 203 72 6 375 1301 432 353 662
Cuts at: 6378 346
Size: 1074
Fragments arranged by size:
1301 1074 1041 725 662 432 375 353
304 208 203 198 99 72 47 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 1501 3247 1501
Size: 1746 5360
EcoO109I rG'GnC_Cy
Cuts at: 1284 1975 2467 2829 3752 4245 1284
Size: 691 492 362 923 493 4145
Fragments arranged by size:
4145 923 691 493 492 362
EcoRI G'AATT_C
Cuts at: 4110 4110
Size: 7106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3495 3579 3702 5125 3495
Size: 84 123 1423 5476
Fragments arranged by size:
5476 1423 123 84
FspI TGC'GCA
Cuts at: 586 4887 586
Size: 4301 2805
FspAI rTGC'GCAy
Cuts at: 586 586
Size: 7106
HaeII r_GCGC'y
Cuts at: 145 957 2108 2570 3289 4540 4788 6018 6789
Size: 812 1151 462 719 1251 248 1230 771
Cuts at: 6789 145
Size: 462
Fragments arranged by size:
1251 1230 1151 812 771 719 462 462
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 1435 1467 1528 1560 2451 2483 2644
Size: 32 127 32 61 32 891 32 161
Cuts at: 2644 2676 2954 2986 3501 3533 3657 3689 4948
Size: 32 278 32 515 32 124 32 1259
Cuts at: 4948 4978 4980 5010 1276
Size: 30 2 30 3372
Fragments arranged by size:
3372 1259 891 515 278 161 127 124
61 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 369 1143 3453 3879 4145 369
Size: 774 2310 426 266 3330
Fragments arranged by size:
3330 2310 774 426 266
HindIII A'AGCT_T
Cuts at: 3357 3357
Size: 7106
KasI G'GCGC_C
Cuts at: 3285 4536 4784 3285
Size: 1251 248 5607
Fragments arranged by size:
5607 1251 248
KpnI G_GTAC'C
Cuts at: 4126 4126
Size: 7106
MluI A'CGCG_T
Cuts at: 6539 6539
Size: 7106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2403 3557 3767 5865 6049 2403
Size: 1154 210 2098 184 3460
Fragments arranged by size:
3460 2098 1154 210 184
MscI TGG'CCA
Cuts at: 683 890 3430 4867 683
Size: 207 2540 1437 2922
Fragments arranged by size:
2922 2540 1437 207
MslI CAynn'nnrTG
Cuts at: 494 898 1681 1756 2671 3913 5095 5222 494
Size: 404 783 75 915 1242 1182 127 2378
Fragments arranged by size:
2378 1242 1182 915 783 404 127 75
MspA1I CmG'CkG
Cuts at: 167 1964 3762 4217 4531 4891 5675 5920 167
Size: 1797 1798 455 314 360 784 245 1353
Fragments arranged by size:
1798 1797 1353 784 455 360 314 245
NaeI GCC'GGC
Cuts at: 129 5287 129
Size: 5158 1948
NarI GG'CG_CC
Cuts at: 3286 4537 4785 3286
Size: 1251 248 5607
Fragments arranged by size:
5607 1251 248
NcoI C'CATG_G
Cuts at: 2111 2111
Size: 7106
NdeI CA'TA_TG
Cuts at: 3093 3093
Size: 7106
NgoMIV G'CCGG_C
Cuts at: 127 5285 127
Size: 5158 1948
NruI TCG'CGA
Cuts at: 1741 1741
Size: 7106
NsiI A_TGCA'T
Cuts at: 1669 1669
Size: 7106
NspI r_CATG'y
Cuts at: 996 1671 1761 3966 4159 4659 6262 996
Size: 675 90 2205 193 500 1603 1840
Fragments arranged by size:
2205 1840 1603 675 500 193 90
PasI CC'CwG_GG
Cuts at: 2603 2603
Size: 7106
PciI A'CATG_T
Cuts at: 1757 3962 6258 1757
Size: 2205 2296 2605
Fragments arranged by size:
2605 2296 2205
PflMI CCAn_nnn'nTGG
Cuts at: 2418 2418
Size: 7106
PfoI T'CCnGG_A
Cuts at: 516 3846 516
Size: 3330 3776
PmeI GTTT'AAAC
Cuts at: 4105 4105
Size: 7106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5552 5584 5552
Size: 32 7074
PpuMI rG'GwC_Cy
Cuts at: 1284 2467 1284
Size: 1183 5923
PstI C_TGCA'G
Cuts at: 178 2769 4153 4838 178
Size: 2591 1384 685 2446
Fragments arranged by size:
2591 2446 1384 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7106
PvuII CAG'CTG
Cuts at: 167 4531 4891 167
Size: 4364 360 2382
Fragments arranged by size:
4364 2382 360
RsrII CG'GwC_CG
Cuts at: 5301 5301
Size: 7106
SacI G_AGCT'C
Cuts at: 2861 4120 2861
Size: 1259 5847
SalI G'TCGA_C
Cuts at: 4143 4143
Size: 7106
SapI GCTCTTCn'nnn_
Cuts at: 5129 5339 5129
Size: 210 6896
SbfI CC_TGCA'GG
Cuts at: 4153 4153
Size: 7106
ScaI AGT'ACT
Cuts at: 1594 2149 2329 6623 1594
Size: 555 180 4294 2077
Fragments arranged by size:
4294 2077 555 180
SfcI C'TryA_G
Cuts at: 33 174 456 2765 2794 2862 4149 4834 5802
Size: 141 282 2309 29 68 1287 685 968
Cuts at: 5802 5993 33
Size: 191 1146
Fragments arranged by size:
2309 1287 1146 968 685 282 191 141
68 29
SfoI GGC'GCC
Cuts at: 3287 4538 4786 3287
Size: 1251 248 5607
Fragments arranged by size:
5607 1251 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7106
SmaI CCC'GGG
Cuts at: 4128 4128
Size: 7106
SmlI C'TyrA_G
Cuts at: 1629 2925 3484 3676 3687 4261 5176 5890 6152
Size: 1296 559 192 11 574 915 714 262
Cuts at: 6152 1629
Size: 2583
Fragments arranged by size:
2583 1296 915 714 574 559 262 192
11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7106
SphI G_CATG'C
Cuts at: 996 1671 4159 4659 996
Size: 675 2488 500 3443
Fragments arranged by size:
3443 2488 675 500
SspI AAT'ATT
Cuts at: 3405 3405
Size: 7106
StyI C'CwwG_G
Cuts at: 408 818 1502 1659 1920 2111 2481 2553 3281
Size: 410 684 157 261 191 370 72 728
Cuts at: 3281 4240 4594 408
Size: 959 354 2920
Fragments arranged by size:
2920 959 728 684 410 370 354 261
191 157 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 1390 2869 3370 4689 4822 5281 5490 423
Size: 967 1479 501 1319 133 459 209 2039
Fragments arranged by size:
2039 1479 1319 967 501 459 209 133
TatI w'GTAC_w
Cuts at: 1592 2147 2327 6621 1592
Size: 555 180 4294 2077
Fragments arranged by size:
4294 2077 555 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1999 2031 3052 3084 6623 6655 1999
Size: 32 1021 32 3539 32 2450
Fragments arranged by size:
3539 2450 1021 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4903 4903
Size: 7106
XbaI T'CTAG_A
Cuts at: 4137 4137
Size: 7106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1332 2419 2796 3327 4601 1332
Size: 1087 377 531 1274 3837
Fragments arranged by size:
3837 1274 1087 531 377
XmaI C'CCGG_G
Cuts at: 4126 4126
Size: 7106
ZraI GAC'GTC
Cuts at: 761 761
Size: 7106
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI ApaI AscI BbvCI BmtI Bpu10I
BsiWI BsmBI BsrGI BstXI BstZ17I EcoRV FseI HpaI
MfeI NheI NotI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI