Restriction map for
pF1KA0835
(Circular) (Six-base) MAPSORT of: pF1KA0835.seq Check: 5632 from: 1 to: 6476
pF1KA0835 6476 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 704 704
Size: 6476
AatII G_ACGT'C
Cuts at: 466 466
Size: 6476
AccI GT'mk_AC
Cuts at: 2096 3514 2096
Size: 1418 5058
Acc65I G'GTAC_C
Cuts at: 3492 3492
Size: 6476
AclI AA'CG_TT
Cuts at: 6316 6316
Size: 6476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 521 653 685 1288 1579 2402 2485 2944
Size: 157 132 32 603 291 823 83 459
Cuts at: 2944 3244 4301 4733 5480 5748 364
Size: 300 1057 432 747 268 1092
Fragments arranged by size:
1092 1057 823 747 603 459 432 300
291 268 157 132 83 32
AfeI AGC'GCT
Cuts at: 799 799
Size: 6476
AflIII A'CryG_T
Cuts at: 3095 3395 3828 4932 5909 3095
Size: 300 433 1104 977 3662
Fragments arranged by size:
3662 1104 977 433 300
AgeI A'CCGG_T
Cuts at: 4864 4864
Size: 6476
AleI CACnn'nnGTG
Cuts at: 400 400
Size: 6476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1918 2871 1918
Size: 953 5523
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2539 2571 2539
Size: 32 6444
AlwNI CAG_nnn'CTG
Cuts at: 682 810 825 1033 1198 2171 3781 5348 682
Size: 128 15 208 165 973 1610 1567 1810
Fragments arranged by size:
1810 1610 1567 973 208 165 128 15
ApaI G_GGCC'C
Cuts at: 1099 1099
Size: 6476
ApaLI G'TGCA_C
Cuts at: 691 5246 691
Size: 4555 1921
ApoI r'AATT_y
Cuts at: 2412 3363 3480 5829 6085 2412
Size: 951 117 2349 256 2803
Fragments arranged by size:
2803 2349 951 256 117
AseI AT'TA_AT
Cuts at: 20 20
Size: 6476
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6476
AvaI C'yCGr_G
Cuts at: 490 1229 1762 2770 2952 3496 490
Size: 739 533 1008 182 544 3470
Fragments arranged by size:
3470 1008 739 544 533 182
BamHI G'GATC_C
Cuts at: 532 1520 1730 3501 532
Size: 988 210 1771 3507
Fragments arranged by size:
3507 1771 988 210
BanI G'GyrC_C
Cuts at: 248 2742 3492 3906 4154 4189 248
Size: 2494 750 414 248 35 2535
Fragments arranged by size:
2535 2494 750 414 248 35
BanII G_rGCy'C
Cuts at: 138 514 1091 1099 2810 3490 4520 138
Size: 376 577 8 1711 680 1030 2094
Fragments arranged by size:
2094 1711 1030 680 577 376 8
BbeI G_GCGC'C
Cuts at: 3910 4158 3910
Size: 248 6228
BbsI GAAGACnn'nnnn_
Cuts at: 131 1058 131
Size: 927 5549
BbvCI CC'TCA_GC
Cuts at: 190 302 2047 190
Size: 112 1745 4619
Fragments arranged by size:
4619 1745 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3450 3484 3450
Size: 34 6442
BciVI GTATCCnnnnn_n'
Cuts at: 2441 4374 5135 6476 2441
Size: 1933 761 1341 2441
Fragments arranged by size:
2441 1933 1341 761
BclI T'GATC_A
Cuts at: 3996 3996
Size: 6476
BfrBI ATG'CAT
Cuts at: 1545 1545
Size: 6476
BglI GCCn_nnn'nGGC
Cuts at: 1078 1270 1331 1499 1825 1078
Size: 192 61 168 326 5729
Fragments arranged by size:
5729 326 192 168 61
BglII A'GATC_T
Cuts at: 1532 3200 3991 6028 1532
Size: 1668 791 2037 1980
Fragments arranged by size:
2037 1980 1668 791
BlpI GC'TnA_GC
Cuts at: 308 3588 308
Size: 3280 3196
Bme1580I G_kGCm'C
Cuts at: 227 695 1099 1567 2641 2788 4101 4194 5250
Size: 468 404 468 1074 147 1313 93 1056
Cuts at: 5250 227
Size: 1453
Fragments arranged by size:
1453 1313 1074 1056 468 468 404 147
93
BmgBI CAC'GTC
Cuts at: 228 3096 228
Size: 2868 3608
BmrI ACTGGGnnnn_n'
Cuts at: 813 1103 2253 2782 3688 3769 4104 6248 813
Size: 290 1150 529 906 81 335 2144 1041
Fragments arranged by size:
2144 1150 1041 906 529 335 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1164 1863 3177 4843 1164
Size: 699 1314 1666 2797
Fragments arranged by size:
2797 1666 1314 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 313 463 847 1270 1858 3301 3454 228
Size: 85 150 384 423 588 1443 153 3250
Fragments arranged by size:
3250 1443 588 423 384 153 150 85
Bpu10I CC'TnA_GC
Cuts at: 190 219 302 1085 1883 2047 3295 190
Size: 29 83 783 798 164 1248 3371
Fragments arranged by size:
3371 1248 798 783 164 83 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2139 2617 3652 4531 5023 5321 2139
Size: 478 1035 879 492 298 3294
Fragments arranged by size:
3294 1035 879 492 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 166 631 3137 6180 166
Size: 465 2506 3043 462
Fragments arranged by size:
3043 2506 465 462
BsaAI yAC'GTr
Cuts at: 1596 3829 1596
Size: 2233 4243
BsaBI GATnn'nnATC
Cuts at: 6 3990 6027 6
Size: 3984 2037 455
Fragments arranged by size:
3984 2037 455
BsaHI Gr'CG_yC
Cuts at: 410 463 1973 2204 3907 4155 4857 6353 410
Size: 53 1510 231 1703 248 702 1496 533
Fragments arranged by size:
1703 1510 1496 702 533 248 231 53
BsaWI w'CCGG_w
Cuts at: 2693 2999 3665 4186 4864 5138 5285 5696 2693
Size: 306 666 521 678 274 147 411 3473
Fragments arranged by size:
3473 678 666 521 411 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1162 1192 2051 2081 2413 2443 2539 2569 2723
Size: 30 859 30 332 30 96 30 154
Cuts at: 2723 2753 3385 3415 1162
Size: 30 632 30 4223
Fragments arranged by size:
4223 859 632 332 154 96 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 552 745 787 859 898 907 910 913 916
Size: 193 42 72 39 9 3 3 3
Cuts at: 916 919 928 931 934 937 964 967 982
Size: 3 9 3 3 3 27 3 15
Cuts at: 982 985 988 1003 1006 1027 1030 1033 1036
Size: 3 3 15 3 21 3 3 3
Cuts at: 1036 2347 2359 2434 3127 3195 3250 3253 3271
Size: 1311 12 75 693 68 55 3 18
Cuts at: 3271 552
Size: 3757
Fragments arranged by size:
3757 1311 693 193 75 72 68 55
42 39 27 21 18 15 15 12
9 9 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 1329 2027 2071 2158 2210 2236 3901 4659 5236
Size: 698 44 87 52 26 1665 758 577
Cuts at: 5236 5732 1329
Size: 496 2073
Fragments arranged by size:
2073 1665 758 698 577 496 87 52
44 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1563 1967 3205 1563
Size: 404 1238 4834
Fragments arranged by size:
4834 1238 404
BsiEI CG_ry'CG
Cuts at: 104 1728 4018 4064 5272 104
Size: 1624 2290 46 1208 1308
Fragments arranged by size:
2290 1624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 138 314 695 1091 3385 3490 4268 4458 5250
Size: 176 381 396 2294 105 778 190 792
Cuts at: 5250 138
Size: 1364
Fragments arranged by size:
2294 1364 792 778 396 381 190 176
105
BsmBI CGTCTCn'nnnn_
Cuts at: 454 454
Size: 6476
Bsp1286I G_dGCh'C
Cuts at: 138 227 314 514 695 1091 1099 1567 2641
Size: 89 87 200 181 396 8 468 1074
Cuts at: 2641 2788 2810 3385 3490 4101 4194 4268 4458
Size: 147 22 575 105 611 93 74 190
Cuts at: 4458 4520 5250 138
Size: 62 730 1364
Fragments arranged by size:
1364 1074 730 611 575 468 396 200
190 181 147 105 93 89 87 74
62 22 8
BspEI T'CCGG_A
Cuts at: 5696 5696
Size: 6476
BspHI T'CATG_A
Cuts at: 5652 5652
Size: 6476
BspMI ACCTGCnnnn'nnnn_
Cuts at: 704 2950 3526 3740 4042 4423 704
Size: 2246 576 214 302 381 2757
Fragments arranged by size:
2757 2246 576 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1148 2424 4768 4822 6306 6471 50
Size: 1098 1276 2344 54 1484 165 55
Fragments arranged by size:
2344 1484 1276 1098 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 4388 1640
Size: 2748 3728
BsrFI r'CCGG_y
Cuts at: 1787 2294 4474 4655 4864 1787
Size: 507 2180 181 209 3399
Fragments arranged by size:
3399 2180 507 209 181
BssSI C'ACGA_G
Cuts at: 2714 4747 5105 2714
Size: 2033 358 4085
Fragments arranged by size:
4085 2033 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1292 2171 3031 3853 1292
Size: 879 860 822 3915
Fragments arranged by size:
3915 879 860 822
BstBI TT'CG_AA
Cuts at: 4837 4837
Size: 6476
BstEII G'GTnAC_C
Cuts at: 1854 1854
Size: 6476
BstXI CCAn_nnnn'nTGG
Cuts at: 1836 2980 1836
Size: 1144 5332
BstYI r'GATC_y
Cuts at: 1 532 1398 1520 1532 1698 1730 3137 3200
Size: 531 866 122 12 166 32 1407 63
Cuts at: 3200 3501 3968 3991 4326 4572 5584 5670 5682
Size: 301 467 23 335 246 1012 86 12
Cuts at: 5682 6028 1
Size: 346 449
Fragments arranged by size:
1407 1012 866 531 467 449 346 335
301 246 166 122 86 63 32 23
12 12
Bsu36I CC'TnA_GG
Cuts at: 1735 2342 1735
Size: 607 5869
BtgI C'CryG_G
Cuts at: 3179 3209 3179
Size: 30 6446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 469 582 1245 4426 4607 4770 469
Size: 113 663 3181 181 163 2175
Fragments arranged by size:
3181 2175 663 181 163 113
BtsI GCAGTG_nn'
Cuts at: 393 554 1997 3719 393
Size: 161 1443 1722 3150
Fragments arranged by size:
3150 1722 1443 161
DraI TTT'AAA
Cuts at: 3475 3475
Size: 6476
DraIII CAC_nnn'GTG
Cuts at: 318 318
Size: 6476
DrdI GACnn_nn'nnGTC
Cuts at: 4182 5040 4182
Size: 858 5618
EaeI y'GGCC_r
Cuts at: 275 668 1458 1826 2532 2664 2864 2970 4061
Size: 393 790 368 706 132 200 106 1091
Cuts at: 4061 4235 4626 4653 5998 275
Size: 174 391 27 1345 753
Fragments arranged by size:
1345 1091 790 753 706 393 391 368
200 174 132 106 27
EagI C'GGCC_G
Cuts at: 4061 4061
Size: 6476
EarI CTCTTCn'nnn_
Cuts at: 277 629 644 754 875 905 929 935 941
Size: 352 15 110 121 30 24 6 6
Cuts at: 941 953 959 974 980 992 998 1004 1375
Size: 12 6 15 6 12 6 6 371
Cuts at: 1375 2402 4499 4709 277
Size: 1027 2097 210 2044
Fragments arranged by size:
2097 2044 1027 371 352 210 121 110
30 24 15 15 12 12 6 6
6 6 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3319 4684 4994 5140 6286 3319
Size: 1365 310 146 1146 3509
Fragments arranged by size:
3509 1365 1146 310 146
EcoICRI GAG'CTC
Cuts at: 136 1089 3488 136
Size: 953 2399 3124
Fragments arranged by size:
3124 2399 953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 313 364 463 521 653 685 847 1270
Size: 85 51 99 58 132 32 162 423
Cuts at: 1270 1288 1579 1858 2402 2485 2944 3244 3301
Size: 18 291 279 544 83 459 300 57
Cuts at: 3301 3454 4301 4733 5480 5748 228
Size: 153 847 432 747 268 956
Fragments arranged by size:
956 847 747 544 459 432 423 300
291 279 268 162 153 132 99 85
83 58 57 51 32 18
EcoNI CCTnn'n_nnAGG
Cuts at: 700 1252 2329 2838 3298 700
Size: 552 1077 509 460 3878
Fragments arranged by size:
3878 1077 552 509 460
EcoO109I rG'GnC_Cy
Cuts at: 153 163 679 744 807 1080 1095 1767 2064
Size: 10 516 65 63 273 15 672 297
Cuts at: 2064 2766 2888 3066 3303 3411 3426 3615 153
Size: 702 122 178 237 108 15 189 3014
Fragments arranged by size:
3014 702 672 516 297 273 237 189
178 122 108 65 63 15 15 10
EcoRI G'AATT_C
Cuts at: 3480 3480
Size: 6476
EcoRV GAT'ATC
Cuts at: 2088 2088
Size: 6476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 765 1875 4495 765
Size: 1110 2620 2746
Fragments arranged by size:
2746 2620 1110
FspI TGC'GCA
Cuts at: 1285 4257 1285
Size: 2972 3504
FspAI rTGC'GCAy
Cuts at: 1285 1285
Size: 6476
HaeII r_GCGC'y
Cuts at: 302 554 801 2217 2234 3910 4158 5180 6159
Size: 252 247 1416 17 1676 248 1022 979
Cuts at: 6159 302
Size: 619
Fragments arranged by size:
1676 1416 1022 979 619 252 248 247
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1162 1194 1416 1448 1683 1715 3385 3417 4318
Size: 32 222 32 235 32 1670 32 901
Cuts at: 4318 4348 4350 4380 1162
Size: 30 2 30 3258
Fragments arranged by size:
3258 1670 901 235 222 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1190 3515 1190
Size: 2325 4151
KasI G'GCGC_C
Cuts at: 3906 4154 3906
Size: 248 6228
KpnI G_GTAC'C
Cuts at: 3496 3496
Size: 6476
MluI A'CGCG_T
Cuts at: 5909 5909
Size: 6476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 560 1187 1459 2384 3080 3182 3428 5147 5331
Size: 627 272 925 696 102 246 1719 184
Cuts at: 5331 560
Size: 1705
Fragments arranged by size:
1719 1705 925 696 627 272 246 184
102
MscI TGG'CCA
Cuts at: 277 1460 1828 2666 2972 4237 277
Size: 1183 368 838 306 1265 2516
Fragments arranged by size:
2516 1265 1183 838 368 306
MslI CAynn'nnrTG
Cuts at: 400 2978 3394 4465 4592 400
Size: 2578 416 1071 127 2284
Fragments arranged by size:
2578 2284 1071 416 127
MspA1I CmG'CkG
Cuts at: 181 194 499 1329 2171 2531 2822 3126 3189
Size: 13 305 830 842 360 291 304 63
Cuts at: 3189 3587 3901 4261 5274 5519 181
Size: 398 314 360 1013 245 1138
Fragments arranged by size:
1138 1013 842 830 398 360 360 314
305 304 291 245 63 13
NaeI GCC'GGC
Cuts at: 4657 4657
Size: 6476
NarI GG'CG_CC
Cuts at: 3907 4155 3907
Size: 248 6228
NcoI C'CATG_G
Cuts at: 3179 3209 3179
Size: 30 6446
NdeI CA'TA_TG
Cuts at: 1776 1776
Size: 6476
NgoMIV G'CCGG_C
Cuts at: 4655 4655
Size: 6476
NsiI A_TGCA'T
Cuts at: 1547 1547
Size: 6476
NspI r_CATG'y
Cuts at: 1905 2127 3399 3529 4029 4936 1905
Size: 222 1272 130 500 907 3445
Fragments arranged by size:
3445 1272 907 500 222 130
PasI CC'CwG_GG
Cuts at: 1814 1814
Size: 6476
PciI A'CATG_T
Cuts at: 3395 4932 3395
Size: 1537 4939
PflMI CCAn_nnn'nTGG
Cuts at: 682 825 682
Size: 143 6333
PfoI T'CCnGG_A
Cuts at: 1180 1247 2911 3431 1180
Size: 67 1664 520 4225
Fragments arranged by size:
4225 1664 520 67
PmeI GTTT'AAAC
Cuts at: 3475 3475
Size: 6476
PmlI CAC'GTG
Cuts at: 1596 1596
Size: 6476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2571 5615 5647 2539
Size: 32 3044 32 3368
Fragments arranged by size:
3368 3044 32 32
PpuMI rG'GwC_Cy
Cuts at: 163 679 807 1767 2064 2766 2888 3066 3411
Size: 516 128 960 297 702 122 178 345
Cuts at: 3411 3426 163
Size: 15 3213
Fragments arranged by size:
3213 960 702 516 345 297 178 128
122 15
PshAI GACnn'nnGTC
Cuts at: 812 812
Size: 6476
PspOMI G'GGCC_C
Cuts at: 1095 1095
Size: 6476
PspXI vC'TCGA_Gb
Cuts at: 1762 1762
Size: 6476
PstI C_TGCA'G
Cuts at: 186 714 2176 3194 3523 4208 186
Size: 528 1462 1018 329 685 2454
Fragments arranged by size:
2454 1462 1018 685 528 329
PvuI CG_AT'CG
Cuts at: 104 1728 104
Size: 1624 4852
PvuII CAG'CTG
Cuts at: 181 194 499 1329 2171 3126 3189 3901 4261
Size: 13 305 830 842 955 63 712 360
Cuts at: 4261 181
Size: 2396
Fragments arranged by size:
2396 955 842 830 712 360 305 63
13
RsrII CG'GwC_CG
Cuts at: 4671 4671
Size: 6476
SacI G_AGCT'C
Cuts at: 138 1091 3490 138
Size: 953 2399 3124
Fragments arranged by size:
3124 2399 953
SalI G'TCGA_C
Cuts at: 3513 3513
Size: 6476
SanDI GG'GwC_CC
Cuts at: 2888 2888
Size: 6476
SapI GCTCTTCn'nnn_
Cuts at: 4499 4709 4499
Size: 210 6266
SbfI CC_TGCA'GG
Cuts at: 3523 3523
Size: 6476
ScaI AGT'ACT
Cuts at: 241 1126 5993 241
Size: 885 4867 724
Fragments arranged by size:
4867 885 724
SexAI A'CCwGG_T
Cuts at: 314 314
Size: 6476
SfcI C'TryA_G
Cuts at: 33 182 576 710 1389 2172 3190 3519 4204
Size: 149 394 134 679 783 1018 329 685
Cuts at: 4204 5197 5388 33
Size: 993 191 1121
Fragments arranged by size:
1121 1018 993 783 685 679 394 329
191 149 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 1825 1825
Size: 6476
SfoI GGC'GCC
Cuts at: 3908 4156 3908
Size: 248 6228
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6476
SmaI CCC'GGG
Cuts at: 1231 2954 3498 1231
Size: 1723 544 4209
Fragments arranged by size:
4209 1723 544
SmlI C'TyrA_G
Cuts at: 1762 2118 2632 3631 4546 5038 5300 1762
Size: 356 514 999 915 492 262 2938
Fragments arranged by size:
2938 999 915 514 492 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6476
SphI G_CATG'C
Cuts at: 1905 2127 3529 4029 1905
Size: 222 1402 500 4352
Fragments arranged by size:
4352 1402 500 222
StuI AGG'CCT
Cuts at: 2272 2272
Size: 6476
StyI C'CwwG_G
Cuts at: 149 566 1829 1850 2477 2750 3179 3209 3610
Size: 417 1263 21 627 273 429 30 401
Cuts at: 3610 3964 149
Size: 354 2661
Fragments arranged by size:
2661 1263 627 429 417 401 354 273
30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1189 1622 1827 2760 2800 2952 3010 4059
Size: 67 433 205 933 40 152 58 1049
Cuts at: 4059 4192 4651 4860 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 1049 933 459 433 209 205 152
133 67 58 40
TatI w'GTAC_w
Cuts at: 239 1124 3398 5991 239
Size: 885 2274 2593 724
Fragments arranged by size:
2593 2274 885 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 2051 2083 2723 2755 5993 6025 304
Size: 32 1715 32 640 32 3238 32 755
Fragments arranged by size:
3238 1715 755 640 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4273 4273
Size: 6476
XbaI T'CTAG_A
Cuts at: 3507 3507
Size: 6476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1821 2079 3165 3971 1821
Size: 258 1086 806 4326
Fragments arranged by size:
4326 1086 806 258
XhoI C'TCGA_G
Cuts at: 1762 1762
Size: 6476
XmaI C'CCGG_G
Cuts at: 1229 2952 3496 1229
Size: 1723 544 4209
Fragments arranged by size:
4209 1723 544
ZraI GAC'GTC
Cuts at: 464 464
Size: 6476
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I ClaI CspCI FseI HindIII HpaI
MfeI NheI NotI NruI PacI PsiI PsrI SacII
SgrAI SnaBI SrfI SspI SwaI XmnI