Restriction map for
pF1KA0767
(Circular) (Six-base) MAPSORT of: pF1KA0767.seq Check: 1664 from: 1 to: 4847
pF1KA0767 4847 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 1022 1022
Size: 4847
AccI GT'mk_AC
Cuts at: 800 1720 1885 800
Size: 920 165 3762
Fragments arranged by size:
3762 920 165
Acc65I G'GTAC_C
Cuts at: 225 1863 225
Size: 1638 3209
AclI AA'CG_TT
Cuts at: 4687 4687
Size: 4847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 523 2672 3104 3851 4119 241
Size: 282 2149 432 747 268 969
Fragments arranged by size:
2149 969 747 432 282 268
AfeI AGC'GCT
Cuts at: 1321 1321
Size: 4847
AflIII A'CryG_T
Cuts at: 803 2199 3303 4280 803
Size: 1396 1104 977 1370
Fragments arranged by size:
1396 1370 1104 977
AgeI A'CCGG_T
Cuts at: 3235 3235
Size: 4847
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 637
Size: 32 4815
AlwNI CAG_nnn'CTG
Cuts at: 447 591 1119 1828 2152 3719 447
Size: 144 528 709 324 1567 1575
Fragments arranged by size:
1575 1567 709 528 324 144
ApaLI G'TGCA_C
Cuts at: 1762 3617 1762
Size: 1855 2992
ApoI r'AATT_y
Cuts at: 1851 4200 4456 1851
Size: 2349 256 2242
Fragments arranged by size:
2349 2242 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4847
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4847
AvaI C'yCGr_G
Cuts at: 242 661 1245 1867 242
Size: 419 584 622 3222
Fragments arranged by size:
3222 622 584 419
BamHI G'GATC_C
Cuts at: 1872 1872
Size: 4847
BanI G'GyrC_C
Cuts at: 225 739 1753 1863 2277 2525 2560 225
Size: 514 1014 110 414 248 35 2512
Fragments arranged by size:
2512 1014 514 414 248 110 35
BanII G_rGCy'C
Cuts at: 401 962 1861 2891 401
Size: 561 899 1030 2357
Fragments arranged by size:
2357 1030 899 561
BbeI G_GCGC'C
Cuts at: 2281 2529 2281
Size: 248 4599
BbsI GAAGACnn'nnnn_
Cuts at: 986 986
Size: 4847
BbvCI CC'TCA_GC
Cuts at: 259 1815 259
Size: 1556 3291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4813
BciVI GTATCCnnnnn_n'
Cuts at: 920 2745 3506 4847 920
Size: 1825 761 1341 920
Fragments arranged by size:
1825 1341 920 761
BclI T'GATC_A
Cuts at: 2367 2367
Size: 4847
BglI GCCn_nnn'nGGC
Cuts at: 1147 1147
Size: 4847
BglII A'GATC_T
Cuts at: 1454 2362 4399 1454
Size: 908 2037 1902
Fragments arranged by size:
2037 1902 908
BlpI GC'TnA_GC
Cuts at: 530 1959 530
Size: 1429 3418
Bme1580I G_kGCm'C
Cuts at: 947 1766 2472 2565 3621 947
Size: 819 706 93 1056 2173
Fragments arranged by size:
2173 1056 819 706 93
BmgBI CAC'GTC
Cuts at: 806 806
Size: 4847
BmrI ACTGGGnnnn_n'
Cuts at: 2059 2140 2475 4619 2059
Size: 81 335 2144 2287
Fragments arranged by size:
2287 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 4847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1087 1305 1308 499
Size: 588 218 3 4038
Fragments arranged by size:
4038 588 218 3
Bpu10I CC'TnA_GC
Cuts at: 112 259 694 1698 1815 112
Size: 147 435 1004 117 3144
Fragments arranged by size:
3144 1004 435 147 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1297 2023 2902 3394 3692 715
Size: 582 726 879 492 298 1870
Fragments arranged by size:
1870 879 726 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1777 4551 1777
Size: 2774 2073
BsaAI yAC'GTr
Cuts at: 639 1374 2200 639
Size: 735 826 3286
Fragments arranged by size:
3286 826 735
BsaBI GATnn'nnATC
Cuts at: 6 2361 4398 6
Size: 2355 2037 455
Fragments arranged by size:
2355 2037 455
BsaHI Gr'CG_yC
Cuts at: 1019 1412 1723 2278 2526 3228 4724 1019
Size: 393 311 555 248 702 1496 1142
Fragments arranged by size:
1496 1142 702 555 393 311 248
BsaWI w'CCGG_w
Cuts at: 317 401 650 1532 2036 2557 3235 3509 3656
Size: 84 249 882 504 521 678 274 147
Cuts at: 3656 4067 317
Size: 411 1097
Fragments arranged by size:
1097 882 678 521 504 411 274 249
147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 778 808 778
Size: 30 4817
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 409 532 679 1302 1420 268
Size: 141 123 147 623 118 3695
Fragments arranged by size:
3695 623 147 141 123 118
BseYI C'CCAG_C
Cuts at: 851 1378 2272 3030 3607 4103 851
Size: 527 894 758 577 496 1595
Fragments arranged by size:
1595 894 758 577 527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 4847
BsiEI CG_ry'CG
Cuts at: 104 1391 1419 2389 2435 3643 104
Size: 1287 28 970 46 1208 1308
Fragments arranged by size:
1308 1287 1208 970 46 28
BsiHKAI G_wGCw'C
Cuts at: 401 1571 1766 1861 2639 2829 3621 401
Size: 1170 195 95 778 190 792 1627
Fragments arranged by size:
1627 1170 792 778 195 190 95
BsmI GAATG_Cn'
Cuts at: 205 840 205
Size: 635 4212
BsmBI CGTCTCn'nnnn_
Cuts at: 717 717
Size: 4847
Bsp1286I G_dGCh'C
Cuts at: 401 947 962 1571 1766 1861 2472 2565 2639
Size: 546 15 609 195 95 611 93 74
Cuts at: 2639 2829 2891 3621 401
Size: 190 62 730 1627
Fragments arranged by size:
1627 730 611 609 546 195 190 95
93 74 62 15
BspEI T'CCGG_A
Cuts at: 401 4067 401
Size: 3666 1181
BspHI T'CATG_A
Cuts at: 4023 4023
Size: 4847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 695 1897 2111 2413 2794 695
Size: 1202 214 302 381 2748
Fragments arranged by size:
2748 1202 381 302 214
BsrBI CCG'CTC
Cuts at: 50 459 624 1743 3139 3193 4677 4842 50
Size: 409 165 1119 1396 54 1484 165 55
Fragments arranged by size:
1484 1396 1119 409 165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 812 2759 570
Size: 242 1947 2658
Fragments arranged by size:
2658 1947 242
BsrFI r'CCGG_y
Cuts at: 747 1386 1390 1414 2845 3026 3235 747
Size: 639 4 24 1431 181 209 2359
Fragments arranged by size:
2359 1431 639 209 181 24 4
BsrGI T'GTAC_A
Cuts at: 827 827
Size: 4847
BssHII G'CGCG_C
Cuts at: 1254 1254
Size: 4847
BssSI C'ACGA_G
Cuts at: 1450 1744 3118 3476 1450
Size: 294 1374 358 2821
Fragments arranged by size:
2821 1374 358 294
BstAPI GCAn_nnn'nTGC
Cuts at: 489 498 2224 489
Size: 9 1726 3112
Fragments arranged by size:
3112 1726 9
BstBI TT'CG_AA
Cuts at: 1598 3208 1598
Size: 1610 3237
BstEII G'GTnAC_C
Cuts at: 352 352
Size: 4847
BstYI r'GATC_y
Cuts at: 1 1454 1872 2339 2362 2697 2943 3955 4041
Size: 1453 418 467 23 335 246 1012 86
Cuts at: 4041 4053 4399 1
Size: 12 346 449
Fragments arranged by size:
1453 1012 467 449 418 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 229 962 1187 1544 1757 229
Size: 733 225 357 213 3319
Fragments arranged by size:
3319 733 357 225 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 894 2797 2978 3141 894
Size: 1903 181 163 2600
Fragments arranged by size:
2600 1903 181 163
BtsI GCAGTG_nn'
Cuts at: 2090 2090
Size: 4847
DraI TTT'AAA
Cuts at: 1236 1846 1236
Size: 610 4237
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2553 3411 1359
Size: 1194 858 2795
Fragments arranged by size:
2795 1194 858
EaeI y'GGCC_r
Cuts at: 293 1388 2432 2606 2997 3024 4369 293
Size: 1095 1044 174 391 27 1345 771
Fragments arranged by size:
1345 1095 1044 771 391 174 27
EagI C'GGCC_G
Cuts at: 1388 2432 1388
Size: 1044 3803
EarI CTCTTCn'nnn_
Cuts at: 2870 3080 2870
Size: 210 4637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 483 939 3055 3365 3511 4657 483
Size: 456 2116 310 146 1146 673
Fragments arranged by size:
2116 1146 673 456 310 146
EcoICRI GAG'CTC
Cuts at: 399 1859 399
Size: 1460 3387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 499 523 1087 1305 1308 2672 3104 3851
Size: 258 24 564 218 3 1364 432 747
Cuts at: 3851 4119 241
Size: 268 969
Fragments arranged by size:
1364 969 747 564 432 268 258 218
24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1623 1623
Size: 4847
EcoO109I rG'GnC_Cy
Cuts at: 264 281 1616 1677 1986 264
Size: 17 1335 61 309 3125
Fragments arranged by size:
3125 1335 309 61 17
EcoRI G'AATT_C
Cuts at: 1851 1851
Size: 4847
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4847
FseI GG_CCGG'CC
Cuts at: 1394 1394
Size: 4847
FspI TGC'GCA
Cuts at: 2628 2628
Size: 4847
HaeII r_GCGC'y
Cuts at: 579 701 1132 1323 1331 1512 2281 2529 3551
Size: 122 431 191 8 181 769 248 1022
Cuts at: 3551 4530 579
Size: 979 896
Fragments arranged by size:
1022 979 896 769 431 248 191 181
122 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 336 368 778 810 1446 1478 2689 2719 2721
Size: 32 410 32 636 32 1211 30 2
Cuts at: 2721 2751 336
Size: 30 2432
Fragments arranged by size:
2432 1211 636 410 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 710 1721 1886 710
Size: 1011 165 3671
Fragments arranged by size:
3671 1011 165
HpaI GTT'AAC
Cuts at: 710 710
Size: 4847
KasI G'GCGC_C
Cuts at: 2277 2525 2277
Size: 248 4599
KpnI G_GTAC'C
Cuts at: 229 1867 229
Size: 1638 3209
MluI A'CGCG_T
Cuts at: 4280 4280
Size: 4847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 101 809 3518 3702 101
Size: 708 2709 184 1246
Fragments arranged by size:
2709 1246 708 184
MscI TGG'CCA
Cuts at: 295 2608 295
Size: 2313 2534
MslI CAynn'nnrTG
Cuts at: 1769 2836 2963 1769
Size: 1067 127 3653
Fragments arranged by size:
3653 1067 127
MspA1I CmG'CkG
Cuts at: 231 465 1008 1485 1819 1958 2272 2632 3645
Size: 234 543 477 334 139 314 360 1013
Cuts at: 3645 3890 231
Size: 245 1188
Fragments arranged by size:
1188 1013 543 477 360 334 314 245
234 139
NaeI GCC'GGC
Cuts at: 1392 3028 1392
Size: 1636 3211
NarI GG'CG_CC
Cuts at: 2278 2526 2278
Size: 248 4599
NcoI C'CATG_G
Cuts at: 1757 1757
Size: 4847
NgoMIV G'CCGG_C
Cuts at: 1390 3026 1390
Size: 1636 3211
NspI r_CATG'y
Cuts at: 1900 2400 3307 1900
Size: 500 907 3440
Fragments arranged by size:
3440 907 500
PciI A'CATG_T
Cuts at: 3303 3303
Size: 4847
PflMI CCAn_nnn'nTGG
Cuts at: 920 920
Size: 4847
PfoI T'CCnGG_A
Cuts at: 305 1064 305
Size: 759 4088
PmeI GTTT'AAAC
Cuts at: 1846 1846
Size: 4847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 4815
PpuMI rG'GwC_Cy
Cuts at: 264 281 1616 1677 264
Size: 17 1335 61 3434
Fragments arranged by size:
3434 1335 61 17
PshAI GACnn'nnGTC
Cuts at: 1415 1415
Size: 4847
PstI C_TGCA'G
Cuts at: 608 1352 1894 2579 608
Size: 744 542 685 2876
Fragments arranged by size:
2876 744 685 542
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4847
PvuII CAG'CTG
Cuts at: 1008 2272 2632 1008
Size: 1264 360 3223
Fragments arranged by size:
3223 1264 360
RsrII CG'GwC_CG
Cuts at: 3042 3042
Size: 4847
SacI G_AGCT'C
Cuts at: 401 1861 401
Size: 1460 3387
SacII CC_GC'GG
Cuts at: 232 232
Size: 4847
SalI G'TCGA_C
Cuts at: 1719 1884 1719
Size: 165 4682
SanDI GG'GwC_CC
Cuts at: 264 281 264
Size: 17 4830
SapI GCTCTTCn'nnn_
Cuts at: 2870 3080 2870
Size: 210 4637
SbfI CC_TGCA'GG
Cuts at: 1894 1894
Size: 4847
ScaI AGT'ACT
Cuts at: 4364 4364
Size: 4847
SfcI C'TryA_G
Cuts at: 33 604 1348 1890 2575 3568 3759 33
Size: 571 744 542 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 571 542 191
SfoI GGC'GCC
Cuts at: 2279 2527 2279
Size: 248 4599
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4847
SmaI CCC'GGG
Cuts at: 244 1869 244
Size: 1625 3222
SmlI C'TyrA_G
Cuts at: 730 1312 2002 2917 3409 3671 730
Size: 582 690 915 492 262 1906
Fragments arranged by size:
1906 915 690 582 492 262
SnaBI TAC'GTA
Cuts at: 1374 1374
Size: 4847
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4847
SphI G_CATG'C
Cuts at: 1900 2400 1900
Size: 500 4347
StuI AGG'CCT
Cuts at: 1777 1777
Size: 4847
StyI C'CwwG_G
Cuts at: 1757 1981 2335 1757
Size: 224 354 4269
Fragments arranged by size:
4269 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1747 2430 2563 3022 3231 1747
Size: 683 133 459 209 3363
Fragments arranged by size:
3363 683 459 209 133
TatI w'GTAC_w
Cuts at: 827 1670 1799 4362 827
Size: 843 129 2563 1312
Fragments arranged by size:
2563 1312 843 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4364 4396 4364
Size: 32 4815
Tth111I GACn'n_nGTC
Cuts at: 990 1359 2644 990
Size: 369 1285 3193
Fragments arranged by size:
3193 1285 369
XbaI T'CTAG_A
Cuts at: 1878 1878
Size: 4847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 562 2342 562
Size: 1780 3067
XmaI C'CCGG_G
Cuts at: 242 1867 242
Size: 1625 3222
XmnI GAAnn'nnTTC
Cuts at: 613 1042 1084 613
Size: 429 42 4376
Fragments arranged by size:
4376 429 42
ZraI GAC'GTC
Cuts at: 1020 1020
Size: 4847
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BmtI BsiWI BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI