Restriction map for
pF1KA0746
(Circular) (Six-base) MAPSORT of: pF1KA0746.seq Check: 8472 from: 1 to: 6050
pF1KA0746 6050 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 3066 3066
Size: 6050
AclI AA'CG_TT
Cuts at: 5890 5890
Size: 6050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 2745 3875 4307 4660 5322 922
Size: 1823 1130 432 353 662 1650
Fragments arranged by size:
1823 1650 1130 662 432 353
AflIII A'CryG_T
Cuts at: 2244 3402 5202 5483 2244
Size: 1158 1800 281 2811
Fragments arranged by size:
2811 1800 1158 281
AleI CACnn'nnGTG
Cuts at: 2393 2393
Size: 6050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1388 1420 1388
Size: 32 6018
AlwNI CAG_nnn'CTG
Cuts at: 203 1346 1743 2356 2721 2768 2950 3355 4793
Size: 1143 397 613 365 47 182 405 1438
Cuts at: 4793 203
Size: 1460
Fragments arranged by size:
1460 1438 1143 613 405 397 365 182
47
ApaLI G'TGCA_C
Cuts at: 4888 4888
Size: 6050
ApoI r'AATT_y
Cuts at: 688 1972 2379 3054 5403 5659 688
Size: 1284 407 675 2349 256 1079
Fragments arranged by size:
2349 1284 1079 675 407 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 450 1409 1703 3070 450
Size: 959 294 1367 3430
Fragments arranged by size:
3430 1367 959 294
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2415 2448 2415
Size: 33 6017
BamHI G'GATC_C
Cuts at: 1824 3075 1824
Size: 1251 4799
BanI G'GyrC_C
Cuts at: 1807 3066 3480 3728 3763 1807
Size: 1259 414 248 35 4094
Fragments arranged by size:
4094 1259 414 248 35
BanII G_rGCy'C
Cuts at: 3064 4094 3064
Size: 1030 5020
BbeI G_GCGC'C
Cuts at: 1811 3484 3732 1811
Size: 1673 248 4129
Fragments arranged by size:
4129 1673 248
BbsI GAAGACnn'nnnn_
Cuts at: 674 1291 1463 674
Size: 617 172 5261
Fragments arranged by size:
5261 617 172
BbvCI CC'TCA_GC
Cuts at: 1004 1004
Size: 6050
BciVI GTATCCnnnnn_n'
Cuts at: 1345 3948 5004 6050 1345
Size: 2603 1056 1046 1345
Fragments arranged by size:
2603 1345 1056 1046
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BfrBI ATG'CAT
Cuts at: 651 1033 651
Size: 382 5668
BglII A'GATC_T
Cuts at: 3565 5602 3565
Size: 2037 4013
BlpI GC'TnA_GC
Cuts at: 1721 3162 1721
Size: 1441 4609
Bme1580I G_kGCm'C
Cuts at: 3675 3768 4892 3675
Size: 93 1124 4833
Fragments arranged by size:
4833 1124 93
BmgBI CAC'GTC
Cuts at: 2957 2957
Size: 6050
BmrI ACTGGGnnnn_n'
Cuts at: 499 1536 3262 3343 3678 5822 499
Size: 1037 1726 81 335 2144 727
Fragments arranged by size:
2144 1726 1037 727 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4417 4417
Size: 6050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 493 1084 1213 1834 2105 2218 2572 2698
Size: 94 591 129 621 271 113 354 126
Cuts at: 2698 2757 2966 399
Size: 59 209 3483
Fragments arranged by size:
3483 621 591 354 271 209 129 126
113 94 59
Bpu10I CC'TnA_GC
Cuts at: 1004 1004
Size: 6050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 976 1630 3226 4105 4819 5117 334
Size: 642 654 1596 879 714 298 1267
Fragments arranged by size:
1596 1267 879 714 654 642 298
BsaI GGTCTCn'nnnn_
Cuts at: 1110 2504 5754 1110
Size: 1394 3250 1406
Fragments arranged by size:
3250 1406 1394
BsaAI yAC'GTr
Cuts at: 3040 3403 3040
Size: 363 5687
BsaBI GATnn'nnATC
Cuts at: 6 3564 5601 6
Size: 3558 2037 455
Fragments arranged by size:
3558 2037 455
BsaHI Gr'CG_yC
Cuts at: 486 1808 3481 3729 4431 5927 486
Size: 1322 1673 248 702 1496 609
Fragments arranged by size:
1673 1496 1322 702 609 248
BsaWI w'CCGG_w
Cuts at: 3239 3760 4438 4849 4996 5270 3239
Size: 521 678 411 147 274 4019
Fragments arranged by size:
4019 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2730 2760 2730
Size: 30 6020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2413 2767 2413
Size: 354 5696
BseYI C'CCAG_C
Cuts at: 838 1171 3475 4233 4898 5306 838
Size: 333 2304 758 665 408 1582
Fragments arranged by size:
2304 1582 758 665 408 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1149 2584 2776 2806 2905 910
Size: 239 1435 192 30 99 4055
Fragments arranged by size:
4055 1435 239 192 99 30
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4868 104
Size: 3488 46 1230 1286
Fragments arranged by size:
3488 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3064 3842 4032 4892 3064
Size: 778 190 860 4222
Fragments arranged by size:
4222 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1810 1810
Size: 6050
Bsp1286I G_dGCh'C
Cuts at: 3064 3675 3768 3842 4032 4094 4892 3064
Size: 611 93 74 190 62 798 4222
Fragments arranged by size:
4222 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 4482 4482
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 854 1097 2798 3100 3314 3616 3997 854
Size: 243 1701 302 214 302 381 2907
Fragments arranged by size:
2907 1701 381 302 302 243 214
BsrBI CCG'CTC
Cuts at: 50 4342 4396 5880 6045 50
Size: 4292 54 1484 165 55
Fragments arranged by size:
4292 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1186 1556 1716 3962 1186
Size: 370 160 2246 3274
Fragments arranged by size:
3274 2246 370 160
BsrFI r'CCGG_y
Cuts at: 4048 4229 4048
Size: 181 5869
BssSI C'ACGA_G
Cuts at: 4321 5029 4321
Size: 708 5342
BstAPI GCAn_nnn'nTGC
Cuts at: 1590 3427 1590
Size: 1837 4213
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstYI r'GATC_y
Cuts at: 1 1200 1824 2214 3075 3542 3565 3900 4146
Size: 1199 624 390 861 467 23 335 246
Cuts at: 4146 4452 4464 4550 5602 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1199 1052 861 624 467 449 390 335
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6050
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 164 1588 2948 2960 3293 164
Size: 1424 1360 12 333 2921
Fragments arranged by size:
2921 1424 1360 333 12
ClaI AT'CG_AT
Cuts at: 2477 2477
Size: 6050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 174 209 543 578 747 782 174
Size: 35 334 35 169 35 5442
Fragments arranged by size:
5442 334 169 35 35 35
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 3756 5100 3756
Size: 1344 4706
EaeI y'GGCC_r
Cuts at: 1766 2414 2526 3635 3809 4200 4227 5572 1766
Size: 648 112 1109 174 391 27 1345 2244
Fragments arranged by size:
2244 1345 1109 648 391 174 112 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 620 4073 4283 620
Size: 3453 210 2387
Fragments arranged by size:
3453 2387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4258 5000 5146 5860 4258
Size: 742 146 714 4448
Fragments arranged by size:
4448 742 714 146
EcoICRI GAG'CTC
Cuts at: 3062 3062
Size: 6050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 493 922 1084 1213 1834 2105 2218 2572
Size: 94 429 162 129 621 271 113 354
Cuts at: 2572 2698 2745 2757 2966 3875 4307 4660 5322
Size: 126 47 12 209 909 432 353 662
Cuts at: 5322 399
Size: 1127
Fragments arranged by size:
1127 909 662 621 432 429 354 353
271 209 162 129 126 113 94 47
12
EcoO109I rG'GnC_Cy
Cuts at: 2159 3189 2159
Size: 1030 5020
EcoRI G'AATT_C
Cuts at: 3054 3054
Size: 6050
EcoRV GAT'ATC
Cuts at: 726 726
Size: 6050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 328 4069 328
Size: 3741 2309
FspI TGC'GCA
Cuts at: 2138 3831 2138
Size: 1693 4357
HaeII r_GCGC'y
Cuts at: 1811 3484 3732 4962 5733 1811
Size: 1673 248 1230 771 2128
Fragments arranged by size:
2128 1673 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 710 742 930 962 1453 1485 1809
Size: 32 550 32 188 32 491 32 324
Cuts at: 1809 1841 2581 2613 3892 3922 3924 3954 128
Size: 32 740 32 1279 30 2 30 2224
Fragments arranged by size:
2224 1279 740 550 491 324 188 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 582 2436 3089 582
Size: 1854 653 3543
Fragments arranged by size:
3543 1854 653
HindIII A'AGCT_T
Cuts at: 2324 2324
Size: 6050
HpaI GTT'AAC
Cuts at: 2436 2436
Size: 6050
KasI G'GCGC_C
Cuts at: 1807 3480 3728 1807
Size: 1673 248 4129
Fragments arranged by size:
4129 1673 248
KpnI G_GTAC'C
Cuts at: 3070 3070
Size: 6050
MfeI C'AATT_G
Cuts at: 1787 1787
Size: 6050
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1425 4809 4993 1425
Size: 3384 184 2482
Fragments arranged by size:
3384 2482 184
MscI TGG'CCA
Cuts at: 1768 2416 2528 3811 1768
Size: 648 112 1283 4007
Fragments arranged by size:
4007 1283 648 112
MslI CAynn'nnrTG
Cuts at: 827 2069 2393 4039 4166 827
Size: 1242 324 1646 127 2711
Fragments arranged by size:
2711 1646 1242 324 127
MspA1I CmG'CkG
Cuts at: 838 1343 1419 2974 2986 3161 3475 3835 4619
Size: 505 76 1555 12 175 314 360 784
Cuts at: 4619 4864 838
Size: 245 2024
Fragments arranged by size:
2024 1555 784 505 360 314 245 175
76 12
NaeI GCC'GGC
Cuts at: 4231 4231
Size: 6050
NarI GG'CG_CC
Cuts at: 1808 3481 3729 1808
Size: 1673 248 4129
Fragments arranged by size:
4129 1673 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6050
NdeI CA'TA_TG
Cuts at: 1608 1608
Size: 6050
NgoMIV G'CCGG_C
Cuts at: 4229 4229
Size: 6050
NsiI A_TGCA'T
Cuts at: 653 1035 653
Size: 382 5668
NspI r_CATG'y
Cuts at: 1073 1431 2248 3103 3603 5206 1073
Size: 358 817 855 500 1603 1917
Fragments arranged by size:
1917 1603 855 817 500 358
PasI CC'CwG_GG
Cuts at: 1142 2601 1142
Size: 1459 4591
PciI A'CATG_T
Cuts at: 2244 5202 2244
Size: 2958 3092
PflMI CCAn_nnn'nTGG
Cuts at: 495 2661 495
Size: 2166 3884
PfoI T'CCnGG_A
Cuts at: 809 2091 2714 809
Size: 1282 623 4145
Fragments arranged by size:
4145 1282 623
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PmlI CAC'GTG
Cuts at: 3040 3040
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 2159 2159
Size: 6050
PsiI TTA'TAA
Cuts at: 827 827
Size: 6050
PstI C_TGCA'G
Cuts at: 159 266 1041 1424 2991 3097 3782 159
Size: 107 775 383 1567 106 685 2427
Fragments arranged by size:
2427 1567 775 685 383 107 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 838 1343 1419 2974 2986 3475 3835 838
Size: 505 76 1555 12 489 360 3053
Fragments arranged by size:
3053 1555 505 489 360 76 12
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 3064 3064
Size: 6050
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 907 2005 5567 907
Size: 1098 3562 1390
Fragments arranged by size:
3562 1390 1098
SfcI C'TryA_G
Cuts at: 33 155 239 262 1037 1420 1548 2987 3093
Size: 122 84 23 775 383 128 1439 106
Cuts at: 3093 3778 4746 4937 33
Size: 685 968 191 1146
Fragments arranged by size:
1439 1146 968 775 685 383 191 128
122 106 84 23
SfoI GGC'GCC
Cuts at: 1809 3482 3730 1809
Size: 1673 248 4129
Fragments arranged by size:
4129 1673 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 3072 3072
Size: 6050
SmlI C'TyrA_G
Cuts at: 313 955 1645 3205 4120 4834 5096 313
Size: 642 690 1560 915 714 262 1267
Fragments arranged by size:
1560 1267 915 714 690 642 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 1073 3103 3603 1073
Size: 2030 500 3520
Fragments arranged by size:
3520 2030 500
SspI AAT'ATT
Cuts at: 2122 2122
Size: 6050
StuI AGG'CCT
Cuts at: 2291 2938 2291
Size: 647 5403
StyI C'CwwG_G
Cuts at: 107 1216 1802 1925 2774 3184 3538 107
Size: 1109 586 123 849 410 354 2619
Fragments arranged by size:
2619 1109 849 586 410 354 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2419 3633 3766 4225 4434 2419
Size: 1214 133 459 209 4035
Fragments arranged by size:
4035 1214 459 209 133
TatI w'GTAC_w
Cuts at: 572 905 2003 5565 572
Size: 333 1098 3562 1057
Fragments arranged by size:
3562 1098 1057 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 2952 2984 5567 5599 2351
Size: 32 569 32 2583 32 2802
Fragments arranged by size:
2802 2583 569 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3847 3847
Size: 6050
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1108 1809 2424 3545 1108
Size: 701 615 1121 3613
Fragments arranged by size:
3613 1121 701 615
XmaI C'CCGG_G
Cuts at: 3070 3070
Size: 6050
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BcgI BglI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI NheI NotI NruI PacI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI