Restriction map for
pF1KA0460
(Circular) (Six-base) MAPSORT of: pF1KA0460.seq Check: 1907 from: 1 to: 7385
pF1KA0460 7385 bp
With 182 enzymes: SgfI *
January 15, 2009 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 765 1491 1497 765
Size: 726 6 6653
Fragments arranged by size:
6653 726 6
AccI GT'mk_AC
Cuts at: 392 3010 4423 392
Size: 2618 1413 3354
Fragments arranged by size:
3354 2618 1413
Acc65I G'GTAC_C
Cuts at: 4401 4401
Size: 7385
AclI AA'CG_TT
Cuts at: 7225 7225
Size: 7385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 643 965 1609 1664 1793 1814 1934 1949
Size: 311 322 644 55 129 21 120 15
Cuts at: 1949 2727 5210 5642 5995 6657 332
Size: 778 2483 432 353 662 1060
Fragments arranged by size:
2483 1060 778 662 644 432 353 322
311 129 120 55 21 15
AfeI AGC'GCT
Cuts at: 601 601
Size: 7385
AflIII A'CryG_T
Cuts at: 4737 6537 6818 4737
Size: 1800 281 5304
Fragments arranged by size:
5304 1800 281
AgeI A'CCGG_T
Cuts at: 2774 2774
Size: 7385
AlwNI CAG_nnn'CTG
Cuts at: 943 1255 1597 3955 4690 6128 943
Size: 312 342 2358 735 1438 2200
Fragments arranged by size:
2358 2200 1438 735 342 312
ApaI G_GGCC'C
Cuts at: 2682 3246 4051 4138 4336 2682
Size: 564 805 87 198 5731
Fragments arranged by size:
5731 805 564 198 87
ApaLI G'TGCA_C
Cuts at: 6223 6223
Size: 7385
ApoI r'AATT_y
Cuts at: 556 721 751 821 2641 3019 3788 4389 6738
Size: 165 30 70 1820 378 769 601 2349
Cuts at: 6738 6994 556
Size: 256 947
Fragments arranged by size:
2349 1820 947 769 601 378 256 165
70 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 7385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
AvaI C'yCGr_G
Cuts at: 2333 3680 4405 2333
Size: 1347 725 5313
Fragments arranged by size:
5313 1347 725
AvrII C'CTAG_G
Cuts at: 4264 4264
Size: 7385
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1184 1217 1184
Size: 33 7352
BamHI G'GATC_C
Cuts at: 333 4410 333
Size: 4077 3308
BanI G'GyrC_C
Cuts at: 2737 3507 4401 4815 5063 5098 2737
Size: 770 894 414 248 35 5024
Fragments arranged by size:
5024 894 770 414 248 35
BanII G_rGCy'C
Cuts at: 1847 2342 2637 2682 3246 4051 4138 4336 4399
Size: 495 295 45 564 805 87 198 63
Cuts at: 4399 5429 1847
Size: 1030 3803
Fragments arranged by size:
3803 1030 805 564 495 295 198 87
63 45
BbeI G_GCGC'C
Cuts at: 4819 5067 4819
Size: 248 7137
BbsI GAAGACnn'nnnn_
Cuts at: 1109 1109
Size: 7385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2127 2161 2127
Size: 34 7351
BciVI GTATCCnnnnn_n'
Cuts at: 1200 5283 6339 7385 1200
Size: 4083 1056 1046 1200
Fragments arranged by size:
4083 1200 1056 1046
BclI T'GATC_A
Cuts at: 3153 4905 3153
Size: 1752 5633
BfrBI ATG'CAT
Cuts at: 868 868
Size: 7385
BglI GCCn_nnn'nGGC
Cuts at: 1819 3476 3627 1819
Size: 1657 151 5577
Fragments arranged by size:
5577 1657 151
BglII A'GATC_T
Cuts at: 199 4900 6937 199
Size: 4701 2037 647
Fragments arranged by size:
4701 2037 647
BlpI GC'TnA_GC
Cuts at: 4007 4497 4007
Size: 490 6895
Bme1580I G_kGCm'C
Cuts at: 1743 2682 3097 3246 3510 4051 4138 4336 5010
Size: 939 415 149 264 541 87 198 674
Cuts at: 5010 5103 6227 1743
Size: 93 1124 2901
Fragments arranged by size:
2901 1124 939 674 541 415 264 198
149 93 87
BmgBI CAC'GTC
Cuts at: 2966 3008 2966
Size: 42 7343
BmrI ACTGGGnnnn_n'
Cuts at: 1029 1243 1415 1639 1646 2861 2893 4597 4678
Size: 214 172 224 7 1215 32 1704 81
Cuts at: 4678 5013 7157 1029
Size: 335 2144 1257
Fragments arranged by size:
2144 1704 1257 1215 335 224 214 172
81 32 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 753 2004 4117 5752 753
Size: 1251 2113 1635 2386
Fragments arranged by size:
2386 2113 1635 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1719 1993 3794 3923 1418
Size: 301 274 1801 129 4880
Fragments arranged by size:
4880 1801 301 274 129
Bpu10I CC'TnA_GC
Cuts at: 1375 2071 4081 1375
Size: 696 2010 4679
Fragments arranged by size:
4679 2010 696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 4561 5440 6154 6452 520
Size: 4041 879 714 298 1453
Fragments arranged by size:
4041 1453 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1962 2415 4280 4302 7089 1962
Size: 453 1865 22 2787 2258
Fragments arranged by size:
2787 2258 1865 453 22
BsaAI yAC'GTr
Cuts at: 3049 4738 3049
Size: 1689 5696
BsaBI GATnn'nnATC
Cuts at: 6 4899 6936 6
Size: 4893 2037 455
Fragments arranged by size:
4893 2037 455
BsaHI Gr'CG_yC
Cuts at: 4816 5064 5766 7262 4816
Size: 248 702 1496 4939
Fragments arranged by size:
4939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 194 2774 4574 5095 5773 6184 6331 6605 194
Size: 2580 1800 521 678 411 147 274 974
Fragments arranged by size:
2580 1800 974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 967 997 1257 1287 1966 1996 3792 3822 967
Size: 30 260 30 679 30 1796 30 4530
Fragments arranged by size:
4530 1796 679 260 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2882 3189 3192 3758 3800 3803 3869 4163 4166
Size: 307 3 566 42 3 66 294 3
Cuts at: 4166 2882
Size: 6101
Fragments arranged by size:
6101 566 307 294 66 42 3 3
3
BseYI C'CCAG_C
Cuts at: 1317 1453 2287 3085 3136 3918 4810 5568 6233
Size: 136 834 798 51 782 892 758 665
Cuts at: 6233 6641 1317
Size: 408 2061
Fragments arranged by size:
2061 892 834 798 782 758 665 408
136 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 1469 1550 1583 681
Size: 788 81 33 6483
Fragments arranged by size:
6483 788 81 33
BsiEI CG_ry'CG
Cuts at: 104 4927 4973 6203 104
Size: 4823 46 1230 1286
Fragments arranged by size:
4823 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1678 2342 3176 3394 3782 3941 4399 5177 5367
Size: 664 834 218 388 159 458 778 190
Cuts at: 5367 6227 1678
Size: 860 2836
Fragments arranged by size:
2836 860 834 778 664 458 388 218
190 159
BsmI GAATG_Cn'
Cuts at: 1556 1556
Size: 7385
BsmBI CGTCTCn'nnnn_
Cuts at: 2716 3201 2716
Size: 485 6900
Bsp1286I G_dGCh'C
Cuts at: 1678 1743 1847 2342 2637 2682 3097 3176 3246
Size: 65 104 495 295 45 415 79 70
Cuts at: 3246 3394 3510 3782 3941 4051 4138 4336 4399
Size: 148 116 272 159 110 87 198 63
Cuts at: 4399 5010 5103 5177 5367 5429 6227 1678
Size: 611 93 74 190 62 798 2836
Fragments arranged by size:
2836 798 611 495 415 295 272 198
190 159 148 116 110 104 93 87
79 74 70 65 63 62 45
BspEI T'CCGG_A
Cuts at: 194 194
Size: 7385
BspHI T'CATG_A
Cuts at: 1394 5817 1394
Size: 4423 2962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 765 1179 1491 1497 4435 4649 4951 5332 765
Size: 414 312 6 2938 214 302 381 2818
Fragments arranged by size:
2938 2818 414 381 312 302 214 6
BsrBI CCG'CTC
Cuts at: 50 2944 5677 5731 7215 7380 50
Size: 2894 2733 54 1484 165 55
Fragments arranged by size:
2894 2733 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5297 5297
Size: 7385
BsrFI r'CCGG_y
Cuts at: 2774 5383 5564 2774
Size: 2609 181 4595
Fragments arranged by size:
4595 2609 181
BssSI C'ACGA_G
Cuts at: 5656 6364 5656
Size: 708 6677
BstAPI GCAn_nnn'nTGC
Cuts at: 4762 4762
Size: 7385
BstBI TT'CG_AA
Cuts at: 3718 5746 3718
Size: 2028 5357
BstEII G'GTnAC_C
Cuts at: 2777 2953 2777
Size: 176 7209
BstXI CCAn_nnnn'nTGG
Cuts at: 1881 2644 1881
Size: 763 6622
BstYI r'GATC_y
Cuts at: 1 199 333 939 1350 3711 4410 4877 4900
Size: 198 134 606 411 2361 699 467 23
Cuts at: 4900 5235 5481 5787 5799 5885 6937 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2361 1052 699 606 467 449 411 335
306 246 198 134 86 23 12
BstZ17I GTA'TAC
Cuts at: 393 393
Size: 7385
Bsu36I CC'TnA_GG
Cuts at: 3656 3656
Size: 7385
BtgI C'CryG_G
Cuts at: 107 1046 2948 4041 4143 107
Size: 939 1902 1093 102 3349
Fragments arranged by size:
3349 1902 1093 939 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5335 5516 5679 5335
Size: 181 163 7041
Fragments arranged by size:
7041 181 163
BtsI GCAGTG_nn'
Cuts at: 499 1600 2238 2388 3846 4185 4628 499
Size: 1101 638 150 1458 339 443 3256
Fragments arranged by size:
3256 1458 1101 638 443 339 150
DraI TTT'AAA
Cuts at: 369 902 1863 3483 4304 4384 369
Size: 533 961 1620 821 80 3370
Fragments arranged by size:
3370 1620 961 821 533 80
DraIII CAC_nnn'GTG
Cuts at: 3945 3945
Size: 7385
DrdI GACnn_nn'nnGTC
Cuts at: 2372 3315 5091 6435 2372
Size: 943 1776 1344 3322
Fragments arranged by size:
3322 1776 1344 943
EaeI y'GGCC_r
Cuts at: 3075 4249 4970 5144 5535 5562 6907 3075
Size: 1174 721 174 391 27 1345 3553
Fragments arranged by size:
3553 1345 1174 721 391 174 27
EagI C'GGCC_G
Cuts at: 4970 4970
Size: 7385
EarI CTCTTCn'nnn_
Cuts at: 575 722 1242 1286 1806 2448 3164 5408 5618
Size: 147 520 44 520 642 716 2244 210
Cuts at: 5618 575
Size: 2342
Fragments arranged by size:
2342 2244 716 642 520 520 210 147
44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5593 6335 6481 7195 5593
Size: 742 146 714 5783
Fragments arranged by size:
5783 742 714 146
EcoICRI GAG'CTC
Cuts at: 2340 4397 2340
Size: 2057 5328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 643 965 1418 1609 1664 1719 1793 1814
Size: 311 322 453 191 55 55 74 21
Cuts at: 1814 1934 1949 1993 2727 3794 3923 5210 5642
Size: 120 15 44 734 1067 129 1287 432
Cuts at: 5642 5995 6657 332
Size: 353 662 1060
Fragments arranged by size:
1287 1067 1060 734 662 453 432 353
322 311 191 129 120 74 55 55
44 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1433 1531 1433
Size: 98 7287
EcoO109I rG'GnC_Cy
Cuts at: 570 2515 2678 2679 3242 3243 3998 4030 4047
Size: 1945 163 1 563 1 755 32 17
Cuts at: 4047 4048 4062 4195 4332 4333 4524 570
Size: 1 14 133 137 1 191 3431
Fragments arranged by size:
3431 1945 755 563 191 163 137 133
32 17 14 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3788 4389 3788
Size: 601 6784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1874 2676 5404 1874
Size: 802 2728 3855
Fragments arranged by size:
3855 2728 802
FspI TGC'GCA
Cuts at: 5166 5166
Size: 7385
HaeII r_GCGC'y
Cuts at: 603 2093 4819 5067 6297 7068 603
Size: 1490 2726 248 1230 771 920
Fragments arranged by size:
2726 1490 1230 920 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 967 999 1074 1106 1120 1152 1257 1289 1488
Size: 32 75 32 14 32 105 32 199
Cuts at: 1488 1520 1966 1998 2230 2262 2410 2442 2698
Size: 32 446 32 232 32 148 32 256
Cuts at: 2698 2730 3427 3459 4199 4231 5227 5257 5259
Size: 32 697 32 740 32 996 30 2
Cuts at: 5259 5289 967
Size: 30 3063
Fragments arranged by size:
3063 996 740 697 446 256 232 199
148 105 75 32 32 32 32 32
32 32 32 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 641 929 3011 3894 4424 641
Size: 288 2082 883 530 3602
Fragments arranged by size:
3602 2082 883 530 288
HindIII A'AGCT_T
Cuts at: 425 1761 425
Size: 1336 6049
KasI G'GCGC_C
Cuts at: 4815 5063 4815
Size: 248 7137
KpnI G_GTAC'C
Cuts at: 4405 4405
Size: 7385
MfeI C'AATT_G
Cuts at: 919 1826 919
Size: 907 6478
MluI A'CGCG_T
Cuts at: 6818 6818
Size: 7385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 893 1898 2846 3569 6144 6328 893
Size: 1005 948 723 2575 184 1950
Fragments arranged by size:
2575 1950 1005 948 723 184
MscI TGG'CCA
Cuts at: 3077 5146 3077
Size: 2069 5316
MslI CAynn'nnrTG
Cuts at: 3356 5374 5501 3356
Size: 2018 127 5240
Fragments arranged by size:
5240 2018 127
MspA1I CmG'CkG
Cuts at: 319 459 1186 3464 4496 4810 5170 5954 6199
Size: 140 727 2278 1032 314 360 784 245
Cuts at: 6199 319
Size: 1505
Fragments arranged by size:
2278 1505 1032 784 727 360 314 245
140
NaeI GCC'GGC
Cuts at: 5566 5566
Size: 7385
NarI GG'CG_CC
Cuts at: 4816 5064 4816
Size: 248 7137
NcoI C'CATG_G
Cuts at: 107 1046 4143 107
Size: 939 3097 3349
Fragments arranged by size:
3349 3097 939
NgoMIV G'CCGG_C
Cuts at: 5564 5564
Size: 7385
NsiI A_TGCA'T
Cuts at: 870 870
Size: 7385
NspI r_CATG'y
Cuts at: 4438 4938 6541 4438
Size: 500 1603 5282
Fragments arranged by size:
5282 1603 500
PasI CC'CwG_GG
Cuts at: 1840 2311 2725 4034 1840
Size: 471 414 1309 5191
Fragments arranged by size:
5191 1309 471 414
PciI A'CATG_T
Cuts at: 6537 6537
Size: 7385
PflMI CCAn_nnn'nTGG
Cuts at: 2311 3106 4122 4144 2311
Size: 795 1016 22 5552
Fragments arranged by size:
5552 1016 795 22
PfoI T'CCnGG_A
Cuts at: 1434 2684 3771 1434
Size: 1250 1087 5048
Fragments arranged by size:
5048 1250 1087
PmeI GTTT'AAAC
Cuts at: 4384 4384
Size: 7385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5831 5863 4262
Size: 32 1537 32 5784
Fragments arranged by size:
5784 1537 32 32
PpuMI rG'GwC_Cy
Cuts at: 3998 4030 4062 4195 3998
Size: 32 32 133 7188
Fragments arranged by size:
7188 133 32 32
PspOMI G'GGCC_C
Cuts at: 2678 3242 4047 4134 4332 2678
Size: 564 805 87 198 5731
Fragments arranged by size:
5731 805 564 198 87
PstI C_TGCA'G
Cuts at: 1176 4432 5117 1176
Size: 3256 685 3444
Fragments arranged by size:
3444 3256 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7385
PvuII CAG'CTG
Cuts at: 319 459 1186 4810 5170 319
Size: 140 727 3624 360 2534
Fragments arranged by size:
3624 2534 727 360 140
RsrII CG'GwC_CG
Cuts at: 5580 5580
Size: 7385
SacI G_AGCT'C
Cuts at: 2342 4399 2342
Size: 2057 5328
SalI G'TCGA_C
Cuts at: 3009 4422 3009
Size: 1413 5972
SanDI GG'GwC_CC
Cuts at: 3998 4195 3998
Size: 197 7188
SapI GCTCTTCn'nnn_
Cuts at: 575 3164 5408 5618 575
Size: 2589 2244 210 2342
Fragments arranged by size:
2589 2342 2244 210
SbfI CC_TGCA'GG
Cuts at: 4432 4432
Size: 7385
ScaI AGT'ACT
Cuts at: 165 6902 165
Size: 6737 648
SfcI C'TryA_G
Cuts at: 33 353 1172 3248 4428 5113 6081 6272 33
Size: 320 819 2076 1180 685 968 191 1146
Fragments arranged by size:
2076 1180 1146 968 819 685 320 191
SfoI GGC'GCC
Cuts at: 4817 5065 4817
Size: 248 7137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
SmaI CCC'GGG
Cuts at: 4407 4407
Size: 7385
SmlI C'TyrA_G
Cuts at: 535 3680 4540 5455 6169 6431 535
Size: 3145 860 915 714 262 1489
Fragments arranged by size:
3145 1489 915 860 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7385
SphI G_CATG'C
Cuts at: 4438 4938 4438
Size: 500 6885
StuI AGG'CCT
Cuts at: 1234 1610 1822 3470 1234
Size: 376 212 1648 5149
Fragments arranged by size:
5149 1648 376 212
StyI C'CwwG_G
Cuts at: 107 1046 1532 3257 3447 3590 3971 4143 4190
Size: 939 486 1725 190 143 381 172 47
Cuts at: 4190 4264 4519 4873 107
Size: 74 255 354 2619
Fragments arranged by size:
2619 1725 939 486 381 354 255 190
172 143 74 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2208 2212 2301 2912 2972 2976 3145 3705 4069
Size: 4 89 611 60 4 169 560 364
Cuts at: 4069 4968 5101 5560 5769 2208
Size: 899 133 459 209 3824
Fragments arranged by size:
3824 899 611 560 459 364 209 169
133 89 60 4 4
TatI w'GTAC_w
Cuts at: 163 303 2293 6900 163
Size: 140 1990 4607 648
Fragments arranged by size:
4607 1990 648 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3259 3291 6902 6934 3259
Size: 32 3611 32 3710
Fragments arranged by size:
3710 3611 32 32
Tth111I GACn'n_nGTC
Cuts at: 1587 5182 1587
Size: 3595 3790
XbaI T'CTAG_A
Cuts at: 4416 4416
Size: 7385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1968 2970 4880 1968
Size: 1002 1910 4473
Fragments arranged by size:
4473 1910 1002
XhoI C'TCGA_G
Cuts at: 3680 3680
Size: 7385
XmaI C'CCGG_G
Cuts at: 4405 4405
Size: 7385
XmnI GAAnn'nnTTC
Cuts at: 709 3724 4274 709
Size: 3015 550 3820
Fragments arranged by size:
3820 3015 550
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BbvCI
BmtI BsiWI BsrGI BssHII ClaI CspCI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI