Restriction map for
pF1KA0386
(Circular) (Six-base) MAPSORT of: pF1KA0386.seq Check: 7669 from: 1 to: 6317
pF1KA0386 6317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 338 1004 2585 3355 338
Size: 666 1581 770 3300
Fragments arranged by size:
3300 1581 770 666
Acc65I G'GTAC_C
Cuts at: 1021 1144 3333 1021
Size: 123 2189 4005
Fragments arranged by size:
4005 2189 123
AclI AA'CG_TT
Cuts at: 6157 6157
Size: 6317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1099 1266 1389 1627 1907 2282 3191 3314
Size: 816 167 123 238 280 375 909 123
Cuts at: 3314 4142 4574 5321 5589 283
Size: 828 432 747 268 1011
Fragments arranged by size:
1011 909 828 816 747 432 375 280
268 238 167 123 123
AfeI AGC'GCT
Cuts at: 2255 2255
Size: 6317
AflIII A'CryG_T
Cuts at: 3669 4773 5750 3669
Size: 1104 977 4236
Fragments arranged by size:
4236 1104 977
AgeI A'CCGG_T
Cuts at: 4705 4705
Size: 6317
AhdI GACnn_n'nnGTC
Cuts at: 3155 3155
Size: 6317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3157 3157
Size: 6317
AlwNI CAG_nnn'CTG
Cuts at: 1093 1684 1989 2191 2853 3622 5189 1093
Size: 591 305 202 662 769 1567 2221
Fragments arranged by size:
2221 1567 769 662 591 305 202
ApaI G_GGCC'C
Cuts at: 146 146
Size: 6317
ApaLI G'TGCA_C
Cuts at: 678 5087 678
Size: 4409 1908
ApoI r'AATT_y
Cuts at: 228 715 779 2007 2524 2699 3250 3321 5670
Size: 487 64 1228 517 175 551 71 2349
Cuts at: 5670 5926 228
Size: 256 619
Fragments arranged by size:
2349 1228 619 551 517 487 256 175
71 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 6317
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 623 3257 3337 623
Size: 2634 80 3603
Fragments arranged by size:
3603 2634 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1005 1038 1005
Size: 33 6284
BamHI G'GATC_C
Cuts at: 118 3342 118
Size: 3224 3093
BanI G'GyrC_C
Cuts at: 571 1021 1144 1833 2348 3333 3747 3995 4030
Size: 450 123 689 515 985 414 248 35
Cuts at: 4030 571
Size: 2858
Fragments arranged by size:
2858 985 689 515 450 414 248 123
35
BanII G_rGCy'C
Cuts at: 146 917 1331 1631 1760 3331 4361 146
Size: 771 414 300 129 1571 1030 2102
Fragments arranged by size:
2102 1571 1030 771 414 300 129
BbeI G_GCGC'C
Cuts at: 3751 3999 3751
Size: 248 6069
BbsI GAAGACnn'nnnn_
Cuts at: 1622 2048 2270 1622
Size: 426 222 5669
Fragments arranged by size:
5669 426 222
BbvCI CC'TCA_GC
Cuts at: 314 1510 314
Size: 1196 5121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2352 2386 2352
Size: 34 6283
BciVI GTATCCnnnnn_n'
Cuts at: 559 1064 4215 4976 6317 559
Size: 505 3151 761 1341 559
Fragments arranged by size:
3151 1341 761 559 505
BclI T'GATC_A
Cuts at: 3837 3837
Size: 6317
BglI GCCn_nnn'nGGC
Cuts at: 141 1218 141
Size: 1077 5240
BglII A'GATC_T
Cuts at: 3832 5869 3832
Size: 2037 4280
BlpI GC'TnA_GC
Cuts at: 1399 1554 1691 2714 3429 1399
Size: 155 137 1023 715 4287
Fragments arranged by size:
4287 1023 715 155 137
Bme1580I G_kGCm'C
Cuts at: 146 682 3942 4035 5091 146
Size: 536 3260 93 1056 1372
Fragments arranged by size:
3260 1372 1056 536 93
BmrI ACTGGGnnnn_n'
Cuts at: 117 2668 3529 3610 3945 6089 117
Size: 2551 861 81 335 2144 345
Fragments arranged by size:
2551 2144 861 345 335 81
BmtI G_CTAG'C
Cuts at: 928 928
Size: 6317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1767 4684 1767
Size: 2917 3400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 175 394 523 547 785 2338 2641 2715
Size: 17 219 129 24 238 1553 303 74
Cuts at: 2715 2824 3064 3070 158
Size: 109 240 6 3405
Fragments arranged by size:
3405 1553 303 240 238 219 129 109
74 24 17 6
Bpu10I CC'TnA_GC
Cuts at: 314 1510 2815 314
Size: 1196 1305 3816
Fragments arranged by size:
3816 1305 1196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1251 1726 1744 2376 3139 3493 4372 4864
Size: 1025 475 18 632 763 354 879 492
Cuts at: 4864 5162 226
Size: 298 1381
Fragments arranged by size:
1381 1025 879 763 632 492 475 354
298 18
BsaI GGTCTCn'nnnn_
Cuts at: 955 6021 955
Size: 5066 1251
BsaAI yAC'GTr
Cuts at: 3670 3670
Size: 6317
BsaBI GATnn'nnATC
Cuts at: 6 368 3831 5868 6
Size: 362 3463 2037 455
Fragments arranged by size:
3463 2037 455 362
BsaHI Gr'CG_yC
Cuts at: 498 558 2792 3748 3996 4698 6194 498
Size: 60 2234 956 248 702 1496 621
Fragments arranged by size:
2234 1496 956 702 621 248 60
BsaWI w'CCGG_w
Cuts at: 3506 4027 4705 4979 5126 5537 3506
Size: 521 678 274 147 411 4286
Fragments arranged by size:
4286 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1279 1309 1279
Size: 30 6287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1134 1393 1639 1759 2143 2146 1134
Size: 259 246 120 384 3 5305
Fragments arranged by size:
5305 384 259 246 120 3
BseYI C'CCAG_C
Cuts at: 708 848 1093 1684 2595 2905 2921 3742 4500
Size: 140 245 591 911 310 16 821 758
Cuts at: 4500 5077 5573 708
Size: 577 496 1452
Fragments arranged by size:
1452 911 821 758 591 577 496 310
245 140 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2346 2395 1249
Size: 1097 49 5171
Fragments arranged by size:
5171 1097 49
BsiEI CG_ry'CG
Cuts at: 104 3859 3905 5113 104
Size: 3755 46 1208 1308
Fragments arranged by size:
3755 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 682 917 1331 1697 1760 3331 4109 4299 5091
Size: 235 414 366 63 1571 778 190 792
Cuts at: 5091 682
Size: 1908
Fragments arranged by size:
1908 1571 792 778 414 366 235 190
63
BsmBI CGTCTCn'nnnn_
Cuts at: 489 489
Size: 6317
Bsp1286I G_dGCh'C
Cuts at: 146 682 917 1331 1631 1697 1760 3331 3942
Size: 536 235 414 300 66 63 1571 611
Cuts at: 3942 4035 4109 4299 4361 5091 146
Size: 93 74 190 62 730 1372
Fragments arranged by size:
1571 1372 730 611 536 414 300 235
190 93 74 66 63 62
BspEI T'CCGG_A
Cuts at: 5537 5537
Size: 6317
BspHI T'CATG_A
Cuts at: 785 2003 5493 785
Size: 1218 3490 1609
Fragments arranged by size:
3490 1609 1218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1298 1421 1647 2042 3367 3581 3883 4264 1298
Size: 123 226 395 1325 214 302 381 3351
Fragments arranged by size:
3351 1325 395 381 302 226 214 123
BsrBI CCG'CTC
Cuts at: 50 238 1277 4609 4663 6147 6312 50
Size: 188 1039 3332 54 1484 165 55
Fragments arranged by size:
3332 1484 1039 188 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4229 4229
Size: 6317
BsrFI r'CCGG_y
Cuts at: 797 4315 4496 4705 797
Size: 3518 181 209 2409
Fragments arranged by size:
3518 2409 209 181
BsrGI T'GTAC_A
Cuts at: 1133 1133
Size: 6317
BssSI C'ACGA_G
Cuts at: 2371 4588 4946 2371
Size: 2217 358 3742
Fragments arranged by size:
3742 2217 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3694 3694
Size: 6317
BstBI TT'CG_AA
Cuts at: 2682 4678 2682
Size: 1996 4321
BstXI CCAn_nnnn'nTGG
Cuts at: 690 690
Size: 6317
BstYI r'GATC_y
Cuts at: 1 118 1897 1986 2444 3342 3809 3832 4167
Size: 117 1779 89 458 898 467 23 335
Cuts at: 4167 4413 5425 5511 5523 5869 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1779 1012 898 467 458 449 346 335
246 117 89 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 251 989 1086 1300 1677 251
Size: 738 97 214 377 4891
Fragments arranged by size:
4891 738 377 214 97
BtgI C'CryG_G
Cuts at: 2787 2840 2787
Size: 53 6264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4267 4448 4611 4267
Size: 181 163 5973
Fragments arranged by size:
5973 181 163
BtsI GCAGTG_nn'
Cuts at: 3560 3560
Size: 6317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3291 3326 3291
Size: 35 6282
DraI TTT'AAA
Cuts at: 3316 3316
Size: 6317
DrdI GACnn_nn'nnGTC
Cuts at: 4023 4881 4023
Size: 858 5459
EaeI y'GGCC_r
Cuts at: 795 1841 2825 3902 4076 4467 4494 5839 795
Size: 1046 984 1077 174 391 27 1345 1273
Fragments arranged by size:
1345 1273 1077 1046 984 391 174 27
EagI C'GGCC_G
Cuts at: 3902 3902
Size: 6317
EarI CTCTTCn'nnn_
Cuts at: 4340 4550 4340
Size: 210 6107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2177 4525 4835 4981 6127 2177
Size: 2348 310 146 1146 2367
Fragments arranged by size:
2367 2348 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 1329 1758 3329 915
Size: 414 429 1571 3903
Fragments arranged by size:
3903 1571 429 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 175 283 394 523 547 785 1099 1266
Size: 17 108 111 129 24 238 314 167
Cuts at: 1266 1389 1627 1907 2282 2338 2641 2715 2824
Size: 123 238 280 375 56 303 74 109
Cuts at: 2824 3064 3070 3191 3314 4142 4574 5321 5589
Size: 240 6 121 123 828 432 747 268
Cuts at: 5589 158
Size: 886
Fragments arranged by size:
886 828 747 432 375 314 303 280
268 240 238 238 167 129 123 123
121 111 109 108 74 56 24 17
6
EcoNI CCTnn'n_nnAGG
Cuts at: 1182 1299 1609 1182
Size: 117 310 5890
Fragments arranged by size:
5890 310 117
EcoO109I rG'GnC_Cy
Cuts at: 142 344 3456 142
Size: 202 3112 3003
Fragments arranged by size:
3112 3003 202
EcoRI G'AATT_C
Cuts at: 715 3321 715
Size: 2606 3711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 609 4336 609
Size: 3727 2590
FspI TGC'GCA
Cuts at: 4098 4098
Size: 6317
HaeII r_GCGC'y
Cuts at: 1094 2257 3751 3999 5021 6000 1094
Size: 1163 1494 248 1022 979 1411
Fragments arranged by size:
1494 1411 1163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 720 752 1279 1311 1537 1569 1582 1614 2931
Size: 32 527 32 226 32 13 32 1317
Cuts at: 2931 2963 3141 3173 4159 4189 4191 4221 720
Size: 32 178 32 986 30 2 30 2816
Fragments arranged by size:
2816 1317 986 527 226 178 32 32
32 32 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 404 1005 2646 3356 404
Size: 601 1641 710 3365
Fragments arranged by size:
3365 1641 710 601
HindIII A'AGCT_T
Cuts at: 539 1914 2094 2431 539
Size: 1375 180 337 4425
Fragments arranged by size:
4425 1375 337 180
KasI G'GCGC_C
Cuts at: 3747 3995 3747
Size: 248 6069
KpnI G_GTAC'C
Cuts at: 1025 1148 3337 1025
Size: 123 2189 4005
Fragments arranged by size:
4005 2189 123
MfeI C'AATT_G
Cuts at: 479 3241 479
Size: 2762 3555
MluI A'CGCG_T
Cuts at: 5750 5750
Size: 6317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1487 3255 4988 5172 1487
Size: 1768 1733 184 2632
Fragments arranged by size:
2632 1768 1733 184
MscI TGG'CCA
Cuts at: 1843 2827 4078 1843
Size: 984 1251 4082
Fragments arranged by size:
4082 1251 984
MslI CAynn'nnrTG
Cuts at: 688 727 2246 3139 4306 4433 688
Size: 39 1519 893 1167 127 2572
Fragments arranged by size:
2572 1519 1167 893 127 39
MspA1I CmG'CkG
Cuts at: 185 804 1806 2470 2599 2619 2823 3428 3742
Size: 619 1002 664 129 20 204 605 314
Cuts at: 3742 4102 5115 5360 185
Size: 360 1013 245 1142
Fragments arranged by size:
1142 1013 1002 664 619 605 360 314
245 204 129 20
NaeI GCC'GGC
Cuts at: 799 4498 799
Size: 3699 2618
NarI GG'CG_CC
Cuts at: 3748 3996 3748
Size: 248 6069
NcoI C'CATG_G
Cuts at: 2840 2840
Size: 6317
NdeI CA'TA_TG
Cuts at: 3234 3234
Size: 6317
NgoMIV G'CCGG_C
Cuts at: 797 4496 797
Size: 3699 2618
NheI G'CTAG_C
Cuts at: 924 924
Size: 6317
NspI r_CATG'y
Cuts at: 3370 3870 4777 3370
Size: 500 907 4910
Fragments arranged by size:
4910 907 500
PasI CC'CwG_GG
Cuts at: 1575 1575
Size: 6317
PciI A'CATG_T
Cuts at: 4773 4773
Size: 6317
PflMI CCAn_nnn'nTGG
Cuts at: 2454 2703 2454
Size: 249 6068
PfoI T'CCnGG_A
Cuts at: 191 563 762 1564 2658 191
Size: 372 199 802 1094 3850
Fragments arranged by size:
3850 1094 802 372 199
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1684 1716 5456 5488 1684
Size: 32 3740 32 2513
Fragments arranged by size:
3740 2513 32 32
PspOMI G'GGCC_C
Cuts at: 142 142
Size: 6317
PstI C_TGCA'G
Cuts at: 1830 1897 2572 3364 4049 1830
Size: 67 675 792 685 4098
Fragments arranged by size:
4098 792 685 675 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 1806 2470 2619 2823 3742 4102 1806
Size: 664 149 204 919 360 4021
Fragments arranged by size:
4021 919 664 360 204 149
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 917 1331 1760 3331 917
Size: 414 429 1571 3903
Fragments arranged by size:
3903 1571 429 414
SalI G'TCGA_C
Cuts at: 1003 3354 1003
Size: 2351 3966
SapI GCTCTTCn'nnn_
Cuts at: 4340 4550 4340
Size: 210 6107
SbfI CC_TGCA'GG
Cuts at: 3364 3364
Size: 6317
ScaI AGT'ACT
Cuts at: 2359 2933 5834 2359
Size: 574 2901 2842
Fragments arranged by size:
2901 2842 574
SexAI A'CCwGG_T
Cuts at: 1048 1048
Size: 6317
SfcI C'TryA_G
Cuts at: 33 339 1826 1893 2568 3360 4045 5038 5229
Size: 306 1487 67 675 792 685 993 191
Cuts at: 5229 33
Size: 1121
Fragments arranged by size:
1487 1121 993 792 685 675 306 191
67
SfoI GGC'GCC
Cuts at: 3749 3997 3749
Size: 248 6069
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 625 3339 625
Size: 2714 3603
SmlI C'TyrA_G
Cuts at: 241 1230 1705 1759 2391 3118 3257 3472 4387
Size: 989 475 54 632 727 139 215 915
Cuts at: 4387 4879 5141 241
Size: 492 262 1417
Fragments arranged by size:
1417 989 915 727 632 492 475 262
215 139 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 3370 3870 3370
Size: 500 5817
StuI AGG'CCT
Cuts at: 1750 3040 1750
Size: 1290 5027
StyI C'CwwG_G
Cuts at: 1017 1313 2840 3451 3805 1017
Size: 296 1527 611 354 3529
Fragments arranged by size:
3529 1527 611 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 2240 2380 3900 4033 4492 4701 433
Size: 1807 140 1520 133 459 209 2049
Fragments arranged by size:
2049 1807 1520 459 209 140 133
TatI w'GTAC_w
Cuts at: 447 662 1133 2357 2931 5832 447
Size: 215 471 1224 574 2901 932
Fragments arranged by size:
2901 1224 932 574 471 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1038 1070 5834 5866 1038
Size: 32 4764 32 1489
Fragments arranged by size:
4764 1489 32 32
Tth111I GACn'n_nGTC
Cuts at: 4114 4114
Size: 6317
XbaI T'CTAG_A
Cuts at: 1905 3348 1905
Size: 1443 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1572 2913 3812 1572
Size: 1341 899 4077
Fragments arranged by size:
4077 1341 899
XhoI C'TCGA_G
Cuts at: 3257 3257
Size: 6317
XmaI C'CCGG_G
Cuts at: 623 3337 623
Size: 2714 3603
XmnI GAAnn'nnTTC
Cuts at: 1727 1727
Size: 6317
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BfrBI
BmgBI BsiWI BsmI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HpaI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI