Restriction map for
pF1KA0348
(Circular) (Six-base) MAPSORT of: pF1KA0348.seq Check: 629 from: 1 to: 7601
pF1KA0348 7601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 990 3285 4151 4230 4560 990
Size: 2295 866 79 330 4031
Fragments arranged by size:
4031 2295 866 330 79
AatII G_ACGT'C
Cuts at: 1574 1574
Size: 7601
AccI GT'mk_AC
Cuts at: 1574 4639 1574
Size: 3065 4536
Acc65I G'GTAC_C
Cuts at: 4101 4617 4101
Size: 516 7085
AclI AA'CG_TT
Cuts at: 7441 7441
Size: 7601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 673 883 1000 1498 1924 3461 4460 5426
Size: 261 210 117 498 426 1537 999 966
Cuts at: 5426 5858 6211 6873 412
Size: 432 353 662 1140
Fragments arranged by size:
1537 1140 999 966 662 498 432 426
353 261 210 117
AflIII A'CryG_T
Cuts at: 284 4953 6753 7034 284
Size: 4669 1800 281 851
Fragments arranged by size:
4669 1800 851 281
AgeI A'CCGG_T
Cuts at: 3451 3451
Size: 7601
AhdI GACnn_n'nnGTC
Cuts at: 1664 3132 4461 1664
Size: 1468 1329 4804
Fragments arranged by size:
4804 1468 1329
AleI CACnn'nnGTG
Cuts at: 367 767 367
Size: 400 7201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 142 4238 142
Size: 4096 3505
AlwNI CAG_nnn'CTG
Cuts at: 388 2144 2913 4152 4906 6344 388
Size: 1756 769 1239 754 1438 1645
Fragments arranged by size:
1756 1645 1438 1239 769 754
ApaI G_GGCC'C
Cuts at: 2767 3908 2767
Size: 1141 6460
ApaLI G'TGCA_C
Cuts at: 6439 6439
Size: 7601
ApoI r'AATT_y
Cuts at: 377 1675 1683 2362 2399 3181 4605 6954 7210
Size: 1298 8 679 37 782 1424 2349 256
Cuts at: 7210 377
Size: 768
Fragments arranged by size:
2349 1424 1298 782 768 679 256 37
8
AseI AT'TA_AT
Cuts at: 20 20
Size: 7601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7601
AvaI C'yCGr_G
Cuts at: 483 1312 1803 2726 3214 3950 4107 4621 483
Size: 829 491 923 488 736 157 514 3463
Fragments arranged by size:
3463 923 829 736 514 491 488 157
BamHI G'GATC_C
Cuts at: 1643 1694 4626 1643
Size: 51 2932 4618
Fragments arranged by size:
4618 2932 51
BanI G'GyrC_C
Cuts at: 140 2104 2626 3499 3673 3774 4080 4101 4214
Size: 1964 522 873 174 101 306 21 113
Cuts at: 4214 4231 4294 4617 5031 5279 5314 140
Size: 17 63 323 414 248 35 2427
Fragments arranged by size:
2427 1964 873 522 414 323 306 248
174 113 101 63 35 21 17
BanII G_rGCy'C
Cuts at: 1449 1491 2767 2852 3338 3740 3908 4408 4438
Size: 42 1276 85 486 402 168 500 30
Cuts at: 4438 4615 5645 1449
Size: 177 1030 3405
Fragments arranged by size:
3405 1276 1030 500 486 402 177 168
85 42 30
BbeI G_GCGC'C
Cuts at: 144 2108 3778 4084 5035 5283 144
Size: 1964 1670 306 951 248 2462
Fragments arranged by size:
2462 1964 1670 951 306 248
BbsI GAAGACnn'nnnn_
Cuts at: 1199 2077 2207 2313 3010 3161 3170 4230 1199
Size: 878 130 106 697 151 9 1060 4570
Fragments arranged by size:
4570 1060 878 697 151 130 106 9
BbvCI CC'TCA_GC
Cuts at: 307 478 3554 307
Size: 171 3076 4354
Fragments arranged by size:
4354 3076 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2704 2738 2704
Size: 34 7567
BciVI GTATCCnnnnn_n'
Cuts at: 5499 6555 7601 5499
Size: 1056 1046 5499
Fragments arranged by size:
5499 1056 1046
BclI T'GATC_A
Cuts at: 1103 2367 2667 5121 1103
Size: 1264 300 2454 3583
Fragments arranged by size:
3583 2454 1264 300
BglI GCCn_nnn'nGGC
Cuts at: 232 618 1088 3631 232
Size: 386 470 2543 4202
Fragments arranged by size:
4202 2543 470 386
BglII A'GATC_T
Cuts at: 5116 7153 5116
Size: 2037 5564
BlpI GC'TnA_GC
Cuts at: 2463 4713 2463
Size: 2250 5351
Bme1580I G_kGCm'C
Cuts at: 614 2631 2767 3908 4097 4217 4234 5226 5319
Size: 2017 136 1141 189 120 17 992 93
Cuts at: 5319 6443 614
Size: 1124 1772
Fragments arranged by size:
2017 1772 1141 1124 992 189 136 120
93 17
BmgBI CAC'GTC
Cuts at: 329 329
Size: 7601
BmrI ACTGGGnnnn_n'
Cuts at: 1197 4453 4539 4813 4894 5229 7373 1197
Size: 3256 86 274 81 335 2144 1425
Fragments arranged by size:
3256 2144 1425 335 274 86 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3407 5968 3244
Size: 163 2561 4877
Fragments arranged by size:
4877 2561 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 221 2579 3118 4096 4159 196
Size: 25 2358 539 978 63 3638
Fragments arranged by size:
3638 2358 978 539 63 25
Bpu10I CC'TnA_GC
Cuts at: 115 307 478 1420 1756 3554 115
Size: 192 171 942 336 1798 4162
Fragments arranged by size:
4162 1798 942 336 192 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 1185 1318 3322 3855 4777 5656 6370 6668
Size: 549 133 2004 533 922 879 714 298
Cuts at: 6668 636
Size: 1569
Fragments arranged by size:
2004 1569 922 879 714 549 533 298
133
BsaI GGTCTCn'nnnn_
Cuts at: 524 1150 1406 2624 3204 3395 3429 3757 7305
Size: 626 256 1218 580 191 34 328 3548
Cuts at: 7305 524
Size: 820
Fragments arranged by size:
3548 1218 820 626 580 328 256 191
34
BsaAI yAC'GTr
Cuts at: 609 4954 609
Size: 4345 3256
BsaBI GATnn'nnATC
Cuts at: 6 5115 7152 6
Size: 5109 2037 455
Fragments arranged by size:
5109 2037 455
BsaHI Gr'CG_yC
Cuts at: 141 1571 2000 2105 3775 4081 5032 5280 5982
Size: 1430 429 105 1670 306 951 248 702
Cuts at: 5982 7478 141
Size: 1496 264
Fragments arranged by size:
1670 1496 1430 951 702 429 306 264
248 105
BsaWI w'CCGG_w
Cuts at: 1697 1742 3451 3647 3861 4790 5311 5989 6400
Size: 45 1709 196 214 929 521 678 411
Cuts at: 6400 6547 6821 1697
Size: 147 274 2477
Fragments arranged by size:
2477 1709 929 678 521 411 274 214
196 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 219 249 1025 1055 2604 2634 4046 4076 219
Size: 30 776 30 1549 30 1412 30 3744
Fragments arranged by size:
3744 1549 1412 776 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 1051 1597 3049 3251 3483 3683 4125 460
Size: 591 546 1452 202 232 200 442 3936
Fragments arranged by size:
3936 1452 591 546 442 232 202 200
BseYI C'CCAG_C
Cuts at: 135 1796 1836 3265 5026 5784 6449 6857 135
Size: 1661 40 1429 1761 758 665 408 879
Fragments arranged by size:
1761 1661 1429 879 758 665 408 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1168 1315 345
Size: 823 147 6631
Fragments arranged by size:
6631 823 147
BsiEI CG_ry'CG
Cuts at: 104 189 2171 2690 5143 5189 6419 104
Size: 85 1982 519 2453 46 1230 1286
Fragments arranged by size:
2453 1982 1286 1230 519 85 46
BsiHKAI G_wGCw'C
Cuts at: 1046 1757 2727 2852 3338 3552 4408 4615 5393
Size: 711 970 125 486 214 856 207 778
Cuts at: 5393 5583 6443 1046
Size: 190 860 2204
Fragments arranged by size:
2204 970 860 856 778 711 486 214
207 190 125
BsiWI C'GTAC_G
Cuts at: 287 287
Size: 7601
BsmBI CGTCTCn'nnnn_
Cuts at: 335 729 3215 335
Size: 394 2486 4721
Fragments arranged by size:
4721 2486 394
Bsp1286I G_dGCh'C
Cuts at: 614 1046 1449 1491 1757 2631 2727 2767 2852
Size: 432 403 42 266 874 96 40 85
Cuts at: 2852 3338 3552 3740 3908 4097 4217 4234 4408
Size: 486 214 188 168 189 120 17 174
Cuts at: 4408 4438 4615 5226 5319 5393 5583 5645 6443
Size: 30 177 611 93 74 190 62 798
Cuts at: 6443 614
Size: 1772
Fragments arranged by size:
1772 874 798 611 486 432 403 266
214 190 189 188 177 174 168 120
96 93 85 74 62 42 40 30
17
BspEI T'CCGG_A
Cuts at: 1697 1742 1697
Size: 45 7556
BspHI T'CATG_A
Cuts at: 2259 4506 6033 2259
Size: 2247 1527 3827
Fragments arranged by size:
3827 2247 1527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 990 2846 3285 3377 4137 4151 4230 4560 4651
Size: 1856 439 92 760 14 79 330 91
Cuts at: 4651 4865 5167 5548 990
Size: 214 302 381 3043
Fragments arranged by size:
3043 1856 760 439 381 330 302 214
92 91 79 14
BsrBI CCG'CTC
Cuts at: 50 2193 5893 5947 7431 7596 50
Size: 2143 3700 54 1484 165 55
Fragments arranged by size:
3700 2143 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5513 5513
Size: 7601
BsrFI r'CCGG_y
Cuts at: 214 3407 3451 5599 5780 214
Size: 3193 44 2148 181 2035
Fragments arranged by size:
3193 2148 2035 181 44
BssHII G'CGCG_C
Cuts at: 181 181
Size: 7601
BssSI C'ACGA_G
Cuts at: 5872 6580 5872
Size: 708 6893
BstAPI GCAn_nnn'nTGC
Cuts at: 972 4152 4978 972
Size: 3180 826 3595
Fragments arranged by size:
3595 3180 826
BstBI TT'CG_AA
Cuts at: 5962 5962
Size: 7601
BstEII G'GTnAC_C
Cuts at: 672 672
Size: 7601
BstXI CCAn_nnnn'nTGG
Cuts at: 769 804 3255 769
Size: 35 2451 5115
Fragments arranged by size:
5115 2451 35
BstYI r'GATC_y
Cuts at: 1 1643 1694 4446 4626 5093 5116 5451 5697
Size: 1642 51 2752 180 467 23 335 246
Cuts at: 5697 6003 6015 6101 7153 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2752 1642 1052 467 449 335 306 246
180 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 4521 4521
Size: 7601
BtgI C'CryG_G
Cuts at: 107 768 3504 3698 4226 107
Size: 661 2736 194 528 3482
Fragments arranged by size:
3482 2736 661 528 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1290 4304 4318 5551 5732 5895 1290
Size: 3014 14 1233 181 163 2996
Fragments arranged by size:
3014 2996 1233 181 163 14
BtsI GCAGTG_nn'
Cuts at: 404 2993 3077 4203 4564 4844 404
Size: 2589 84 1126 361 280 3161
Fragments arranged by size:
3161 2589 1126 361 280 84
DraI TTT'AAA
Cuts at: 4600 4600
Size: 7601
DraIII CAC_nnn'GTG
Cuts at: 630 972 4533 630
Size: 342 3561 3698
Fragments arranged by size:
3698 3561 342
DrdI GACnn_nn'nnGTC
Cuts at: 535 2602 5307 6651 535
Size: 2067 2705 1344 1485
Fragments arranged by size:
2705 2067 1485 1344
EaeI y'GGCC_r
Cuts at: 186 224 505 786 1410 2168 3415 5186 5360
Size: 38 281 281 624 758 1247 1771 174
Cuts at: 5360 5751 5778 7123 186
Size: 391 27 1345 664
Fragments arranged by size:
1771 1345 1247 758 664 624 391 281
281 174 38 27
EagI C'GGCC_G
Cuts at: 186 2168 5186 186
Size: 1982 3018 2601
Fragments arranged by size:
3018 2601 1982
EarI CTCTTCn'nnn_
Cuts at: 428 431 1859 2807 5624 5834 428
Size: 3 1428 948 2817 210 2195
Fragments arranged by size:
2817 2195 1428 948 210 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1046 1780 3358 5809 6551 6697 7411 1046
Size: 734 1578 2451 742 146 714 1236
Fragments arranged by size:
2451 1578 1236 742 734 714 146
EcoICRI GAG'CTC
Cuts at: 2850 3336 4406 4613 2850
Size: 486 1070 207 5838
Fragments arranged by size:
5838 1070 486 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 221 412 673 883 1000 1498 1924 2579
Size: 25 191 261 210 117 498 426 655
Cuts at: 2579 3118 3461 4096 4159 4460 5426 5858 6211
Size: 539 343 635 63 301 966 432 353
Cuts at: 6211 6873 196
Size: 662 924
Fragments arranged by size:
966 924 662 655 635 539 498 432
426 353 343 301 261 210 191 117
63 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1423 3837 4049 1423
Size: 2414 212 4975
Fragments arranged by size:
4975 2414 212
EcoO109I rG'GnC_Cy
Cuts at: 1317 1712 2763 2764 2835 3711 3905 3927 4149
Size: 395 1051 1 71 876 194 22 222
Cuts at: 4149 4179 4344 4740 1317
Size: 30 165 396 4178
Fragments arranged by size:
4178 1051 876 396 395 222 194 165
71 30 22 1
EcoRI G'AATT_C
Cuts at: 377 1675 3181 4605 377
Size: 1298 1506 1424 3373
Fragments arranged by size:
3373 1506 1424 1298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3608 3849 5620 3608
Size: 241 1771 5589
Fragments arranged by size:
5589 1771 241
FspI TGC'GCA
Cuts at: 5382 5382
Size: 7601
HaeII r_GCGC'y
Cuts at: 144 1627 2108 3778 4084 4475 5035 5283 6513
Size: 1483 481 1670 306 391 560 248 1230
Cuts at: 6513 7284 144
Size: 771 461
Fragments arranged by size:
1670 1483 1230 771 560 481 461 391
306 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 582 826 858 1248 1280 1650 1654 1682
Size: 32 244 32 390 32 370 4 28
Cuts at: 1682 1686 2446 2478 2920 2952 3023 3055 3118
Size: 4 760 32 442 32 71 32 63
Cuts at: 3118 3150 3214 3246 3933 3965 4192 4224 4447
Size: 32 64 32 687 32 227 32 223
Cuts at: 4447 4479 5443 5473 5475 5505 550
Size: 32 964 30 2 30 2646
Fragments arranged by size:
2646 964 760 687 442 390 370 244
227 223 71 64 63 32 32 32
32 32 32 32 32 32 32 32
30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 1368 1979 2595 4640 1368
Size: 611 616 2045 4329
Fragments arranged by size:
4329 2045 616 611
KasI G'GCGC_C
Cuts at: 140 2104 3774 4080 5031 5279 140
Size: 1964 1670 306 951 248 2462
Fragments arranged by size:
2462 1964 1670 951 306 248
KpnI G_GTAC'C
Cuts at: 4105 4621 4105
Size: 516 7085
MfeI C'AATT_G
Cuts at: 2879 2879
Size: 7601
MluI A'CGCG_T
Cuts at: 284 7034 284
Size: 6750 851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 821 1541 3328 3472 6360 6544 821
Size: 720 1787 144 2888 184 1878
Fragments arranged by size:
2888 1878 1787 720 184 144
MscI TGG'CCA
Cuts at: 226 507 1412 5362 226
Size: 281 905 3950 2465
Fragments arranged by size:
3950 2465 905 281
MslI CAynn'nnrTG
Cuts at: 367 767 1099 2264 5590 5717 367
Size: 400 332 1165 3326 127 2251
Fragments arranged by size:
3326 2251 1165 400 332 127
MspA1I CmG'CkG
Cuts at: 600 876 1840 1992 2557 4228 4468 4480 4712
Size: 276 964 152 565 1671 240 12 232
Cuts at: 4712 5026 5386 6170 6415 600
Size: 314 360 784 245 1786
Fragments arranged by size:
1786 1671 964 784 565 360 314 276
245 240 232 152 12
NaeI GCC'GGC
Cuts at: 216 5782 216
Size: 5566 2035
NarI GG'CG_CC
Cuts at: 141 2105 3775 4081 5032 5280 141
Size: 1964 1670 306 951 248 2462
Fragments arranged by size:
2462 1964 1670 951 306 248
NcoI C'CATG_G
Cuts at: 107 3504 107
Size: 3397 4204
NdeI CA'TA_TG
Cuts at: 2152 2152
Size: 7601
NgoMIV G'CCGG_C
Cuts at: 214 5780 214
Size: 5566 2035
NotI GC'GGCC_GC
Cuts at: 186 186
Size: 7601
NspI r_CATG'y
Cuts at: 1608 4654 5154 6757 1608
Size: 3046 500 1603 2452
Fragments arranged by size:
3046 2452 1603 500
PasI CC'CwG_GG
Cuts at: 1341 1341
Size: 7601
PciI A'CATG_T
Cuts at: 6753 6753
Size: 7601
PflMI CCAn_nnn'nTGG
Cuts at: 514 3014 4330 514
Size: 2500 1316 3785
Fragments arranged by size:
3785 2500 1316
PfoI T'CCnGG_A
Cuts at: 2741 2951 3656 2741
Size: 210 705 6686
Fragments arranged by size:
6686 705 210
PmeI GTTT'AAAC
Cuts at: 4600 4600
Size: 7601
PmlI CAC'GTG
Cuts at: 609 609
Size: 7601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 854 886 6047 6079 854
Size: 32 5161 32 2376
Fragments arranged by size:
5161 2376 32 32
PpuMI rG'GwC_Cy
Cuts at: 1317 1712 2835 3711 4149 4344 1317
Size: 395 1123 876 438 195 4574
Fragments arranged by size:
4574 1123 876 438 395 195
PsiI TTA'TAA
Cuts at: 3596 3596
Size: 7601
PspOMI G'GGCC_C
Cuts at: 2763 3904 2763
Size: 1141 6460
PspXI vC'TCGA_Gb
Cuts at: 1312 3950 1312
Size: 2638 4963
PstI C_TGCA'G
Cuts at: 1331 2636 4002 4148 4157 4571 4648 5333 1331
Size: 1305 1366 146 9 414 77 685 3599
Fragments arranged by size:
3599 1366 1305 685 414 146 77 9
PvuI CG_AT'CG
Cuts at: 104 2690 104
Size: 2586 5015
PvuII CAG'CTG
Cuts at: 600 1840 1992 2557 4468 4480 5026 5386 600
Size: 1240 152 565 1911 12 546 360 2815
Fragments arranged by size:
2815 1911 1240 565 546 360 152 12
RsrII CG'GwC_CG
Cuts at: 5796 5796
Size: 7601
SacI G_AGCT'C
Cuts at: 2852 3338 4408 4615 2852
Size: 486 1070 207 5838
Fragments arranged by size:
5838 1070 486 207
SacII CC_GC'GG
Cuts at: 4229 4229
Size: 7601
SalI G'TCGA_C
Cuts at: 4638 4638
Size: 7601
SapI GCTCTTCn'nnn_
Cuts at: 5624 5834 5624
Size: 210 7391
SbfI CC_TGCA'GG
Cuts at: 4148 4648 4148
Size: 500 7101
ScaI AGT'ACT
Cuts at: 1908 7118 1908
Size: 5210 2391
SexAI A'CCwGG_T
Cuts at: 626 2614 3326 626
Size: 1988 712 4901
Fragments arranged by size:
4901 1988 712
SfcI C'TryA_G
Cuts at: 33 162 1327 2374 2380 2632 3998 4144 4153
Size: 129 1165 1047 6 252 1366 146 9
Cuts at: 4153 4567 4644 5329 6297 6488 33
Size: 414 77 685 968 191 1146
Fragments arranged by size:
1366 1165 1146 1047 968 685 414 252
191 146 129 77 9 6
SfoI GGC'GCC
Cuts at: 142 2106 3776 4082 5033 5281 142
Size: 1964 1670 306 951 248 2462
Fragments arranged by size:
2462 1964 1670 951 306 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7601
SmaI CCC'GGG
Cuts at: 4623 4623
Size: 7601
SmlI C'TyrA_G
Cuts at: 615 1164 1312 1333 3337 3834 3950 4756 5671
Size: 549 148 21 2004 497 116 806 915
Cuts at: 5671 6385 6647 615
Size: 714 262 1569
Fragments arranged by size:
2004 1569 915 806 714 549 497 262
148 116 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7601
SphI G_CATG'C
Cuts at: 1608 4654 5154 1608
Size: 3046 500 4055
Fragments arranged by size:
4055 3046 500
SspI AAT'ATT
Cuts at: 1893 1893
Size: 7601
StuI AGG'CCT
Cuts at: 715 935 715
Size: 220 7381
StyI C'CwwG_G
Cuts at: 107 440 710 1463 2861 2901 3008 3504 3566
Size: 333 270 753 1398 40 107 496 62
Cuts at: 3566 3706 4097 4484 4735 5089 107
Size: 140 391 387 251 354 2619
Fragments arranged by size:
2619 1398 753 496 391 387 354 333
270 251 140 107 62 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1295 2233 2710 3015 3387 3517 3819 4232 4521
Size: 938 477 305 372 130 302 413 289
Cuts at: 4521 5184 5317 5776 5985 1295
Size: 663 133 459 209 2911
Fragments arranged by size:
2911 938 663 477 459 413 372 305
302 289 209 133 130
TatI w'GTAC_w
Cuts at: 1906 7116 1906
Size: 5210 2391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 1025 1057 2604 2636 4046 4078 7118
Size: 32 774 32 1547 32 1410 32 3040
Cuts at: 7118 7150 219
Size: 32 670
Fragments arranged by size:
3040 1547 1410 774 670 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5398 5398
Size: 7601
XbaI T'CTAG_A
Cuts at: 2574 2586 4632 2574
Size: 12 2046 5543
Fragments arranged by size:
5543 2046 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5096 5096
Size: 7601
XhoI C'TCGA_G
Cuts at: 1312 3950 1312
Size: 2638 4963
XmaI C'CCGG_G
Cuts at: 4621 4621
Size: 7601
XmnI GAAnn'nnTTC
Cuts at: 583 1937 583
Size: 1354 6247
ZraI GAC'GTC
Cuts at: 1572 1572
Size: 7601
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsmI BsrGI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI NheI NruI NsiI PacI PshAI PsrI
SanDI SfiI SgrAI SnaBI SrfI SwaI