Restriction map for
pF1KA0347
(Circular) (Six-base) MAPSORT of: pF1KA0347.seq Check: 6690 from: 1 to: 6878
pF1KA0347 6878 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 537 1849 537
Size: 1312 5566
AccI GT'mk_AC
Cuts at: 3916 3916
Size: 6878
Acc65I G'GTAC_C
Cuts at: 3894 3894
Size: 6878
AclI AA'CG_TT
Cuts at: 6718 6718
Size: 6878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 756 1936 3634 4703 5135 5488 6150 481
Size: 275 1180 1698 1069 432 353 662 1209
Fragments arranged by size:
1698 1209 1180 1069 662 432 353 275
AfeI AGC'GCT
Cuts at: 1952 1952
Size: 6878
AflIII A'CryG_T
Cuts at: 1433 2612 4230 6030 6311 1433
Size: 1179 1618 1800 281 2000
Fragments arranged by size:
2000 1800 1618 1179 281
AleI CACnn'nnGTG
Cuts at: 602 1223 602
Size: 621 6257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2789 2789
Size: 6878
AlwNI CAG_nnn'CTG
Cuts at: 278 579 1188 2698 2743 3157 3666 4183 5621
Size: 301 609 1510 45 414 509 517 1438
Cuts at: 5621 278
Size: 1535
Fragments arranged by size:
1535 1510 1438 609 517 509 414 301
45
ApaI G_GGCC'C
Cuts at: 1420 2227 2555 1420
Size: 807 328 5743
Fragments arranged by size:
5743 807 328
ApaLI G'TGCA_C
Cuts at: 1057 5716 1057
Size: 4659 2219
ApoI r'AATT_y
Cuts at: 124 1082 1100 1652 3576 3882 6231 6487 124
Size: 958 18 552 1924 306 2349 256 515
Fragments arranged by size:
2349 1924 958 552 515 306 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 6878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6878
AvaI C'yCGr_G
Cuts at: 1810 2519 2850 3002 3188 3312 3369 3898 1810
Size: 709 331 152 186 124 57 529 4790
Fragments arranged by size:
4790 709 529 331 186 152 124 57
BamHI G'GATC_C
Cuts at: 3507 3838 3903 3507
Size: 331 65 6482
Fragments arranged by size:
6482 331 65
BanI G'GyrC_C
Cuts at: 801 2715 2808 3207 3894 4308 4556 4591 801
Size: 1914 93 399 687 414 248 35 3088
Fragments arranged by size:
3088 1914 687 414 399 248 93 35
BanII G_rGCy'C
Cuts at: 161 170 1420 1493 2227 2264 2555 3319 3343
Size: 9 1250 73 734 37 291 764 24
Cuts at: 3343 3892 4922 161
Size: 549 1030 2117
Fragments arranged by size:
2117 1250 1030 764 734 549 291 73
37 24 9
BbeI G_GCGC'C
Cuts at: 805 2812 3211 4312 4560 805
Size: 2007 399 1101 248 3123
Fragments arranged by size:
3123 2007 1101 399 248
BbsI GAAGACnn'nnnn_
Cuts at: 404 3833 404
Size: 3429 3449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 667 701 2115 2149 667
Size: 34 1414 34 5396
Fragments arranged by size:
5396 1414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 109 4776 5832 6878 109
Size: 4667 1056 1046 109
Fragments arranged by size:
4667 1056 1046 109
BclI T'GATC_A
Cuts at: 4398 4398
Size: 6878
BfrBI ATG'CAT
Cuts at: 899 899
Size: 6878
BglI GCCn_nnn'nGGC
Cuts at: 2222 2806 3004 2222
Size: 584 198 6096
Fragments arranged by size:
6096 584 198
BglII A'GATC_T
Cuts at: 4393 6430 4393
Size: 2037 4841
BlpI GC'TnA_GC
Cuts at: 3306 3990 3306
Size: 684 6194
Bme1580I G_kGCm'C
Cuts at: 620 1061 1420 2227 2555 2642 2753 4503 4596
Size: 441 359 807 328 87 111 1750 93
Cuts at: 4596 5720 620
Size: 1124 1778
Fragments arranged by size:
1778 1750 1124 807 441 359 328 111
93 87
BmgBI CAC'GTC
Cuts at: 615 1434 2615 3263 3720 615
Size: 819 1181 648 457 3773
Fragments arranged by size:
3773 1181 819 648 457
BmrI ACTGGGnnnn_n'
Cuts at: 134 1195 2613 2647 2828 3361 4090 4171 4506
Size: 1061 1418 34 181 533 729 81 335
Cuts at: 4506 6650 134
Size: 2144 362
Fragments arranged by size:
2144 1418 1061 729 533 362 335 181
81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2037 2949 5245 2037
Size: 912 2296 3670
Fragments arranged by size:
3670 2296 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1527 1590 1727 2007 2024 2746 3115 3140
Size: 1323 63 137 280 17 722 369 25
Cuts at: 3140 3616 204
Size: 476 3466
Fragments arranged by size:
3466 1323 722 476 369 280 137 63
25 17
Bpu10I CC'TnA_GC
Cuts at: 1954 1954
Size: 6878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 4054 4933 5647 5945 875
Size: 3179 879 714 298 1808
Fragments arranged by size:
3179 1808 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 226 2248 6582 226
Size: 2022 4334 522
Fragments arranged by size:
4334 2022 522
BsaAI yAC'GTr
Cuts at: 4231 4231
Size: 6878
BsaBI GATnn'nnATC
Cuts at: 6 4392 6429 6
Size: 4386 2037 455
Fragments arranged by size:
4386 2037 455
BsaHI Gr'CG_yC
Cuts at: 802 982 2098 2809 3208 3251 3362 4309 4557
Size: 180 1116 711 399 43 111 947 248
Cuts at: 4557 5259 6755 802
Size: 702 1496 925
Fragments arranged by size:
1496 1116 947 925 711 702 399 248
180 111 43
BsaWI w'CCGG_w
Cuts at: 321 935 2452 2539 4067 4588 5266 5677 5824
Size: 614 1517 87 1528 521 678 411 147
Cuts at: 5824 6098 321
Size: 274 1101
Fragments arranged by size:
1528 1517 1101 678 614 521 411 274
147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 599 604 629 634 1525 1555 3614 3644 599
Size: 5 25 5 891 30 2059 30 3833
Fragments arranged by size:
3833 2059 891 30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 649 919 1474 1593 1656 1828 2332 3420 649
Size: 270 555 119 63 172 504 1088 4107
Fragments arranged by size:
4107 1088 555 504 270 172 119 63
BseYI C'CCAG_C
Cuts at: 133 170 175 300 1477 1752 1935 1947 2274
Size: 37 5 125 1177 275 183 12 327
Cuts at: 2274 2631 2884 2956 4303 5061 5726 6134 133
Size: 357 253 72 1347 758 665 408 877
Fragments arranged by size:
1347 1177 877 758 665 408 357 327
275 253 183 125 72 37 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1231 1437 1456 2136 2285 888
Size: 343 206 19 680 149 5481
Fragments arranged by size:
5481 680 343 206 149 19
BsiEI CG_ry'CG
Cuts at: 104 4420 4466 5696 104
Size: 4316 46 1230 1286
Fragments arranged by size:
4316 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 662 1061 1208 1493 1586 2539 3892 4670 4860
Size: 399 147 285 93 953 1353 778 190
Cuts at: 4860 5720 662
Size: 860 1820
Fragments arranged by size:
1820 1353 953 860 778 399 285 190
147 93
BsmI GAATG_Cn'
Cuts at: 678 901 1481 2821 678
Size: 223 580 1340 4735
Fragments arranged by size:
4735 1340 580 223
Bsp1286I G_dGCh'C
Cuts at: 161 170 620 662 1061 1208 1420 1493 1586
Size: 9 450 42 399 147 212 73 93
Cuts at: 1586 2227 2264 2539 2555 2642 2753 3319 3343
Size: 641 37 275 16 87 111 566 24
Cuts at: 3343 3892 4503 4596 4670 4860 4922 5720 161
Size: 549 611 93 74 190 62 798 1319
Fragments arranged by size:
1319 798 641 611 566 549 450 399
275 212 190 147 111 93 93 87
74 73 62 42 37 24 16 9
BspEI T'CCGG_A
Cuts at: 935 2452 935
Size: 1517 5361
BspHI T'CATG_A
Cuts at: 1698 3548 5310 1698
Size: 1850 1762 3266
Fragments arranged by size:
3266 1850 1762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 1849 2873 3092 3928 4142 4444 4825 537
Size: 1312 1024 219 836 214 302 381 2590
Fragments arranged by size:
2590 1312 1024 836 381 302 219 214
BsrBI CCG'CTC
Cuts at: 50 3045 5170 5224 6708 6873 50
Size: 2995 2125 54 1484 165 55
Fragments arranged by size:
2995 2125 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1905 4790 1905
Size: 2885 3993
BsrFI r'CCGG_y
Cuts at: 1508 4876 5057 1508
Size: 3368 181 3329
Fragments arranged by size:
3368 3329 181
BsrGI T'GTAC_A
Cuts at: 722 722
Size: 6878
BssSI C'ACGA_G
Cuts at: 946 1207 1579 5149 5857 946
Size: 261 372 3570 708 1967
Fragments arranged by size:
3570 1967 708 372 261
BstAPI GCAn_nnn'nTGC
Cuts at: 3666 4255 3666
Size: 589 6289
BstBI TT'CG_AA
Cuts at: 415 5239 415
Size: 4824 2054
BstEII G'GTnAC_C
Cuts at: 595 2600 595
Size: 2005 4873
BstXI CCAn_nnnn'nTGG
Cuts at: 510 789 3720 510
Size: 279 2931 3668
Fragments arranged by size:
3668 2931 279
BstYI r'GATC_y
Cuts at: 1 716 1259 1502 2401 2542 3507 3838 3903
Size: 715 543 243 899 141 965 331 65
Cuts at: 3903 4370 4393 4728 4974 5280 5292 5378 6430
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6430 1
Size: 449
Fragments arranged by size:
1052 965 899 715 543 467 449 335
331 306 246 243 141 86 65 23
12
Bsu36I CC'TnA_GG
Cuts at: 532 1181 3107 532
Size: 649 1926 4303
Fragments arranged by size:
4303 1926 649
BtgI C'CryG_G
Cuts at: 161 251 629 1366 1778 2753 3023 3125 161
Size: 90 378 737 412 975 270 102 3914
Fragments arranged by size:
3914 975 737 412 378 270 102 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 791 4828 5009 5172 791
Size: 4037 181 163 2497
Fragments arranged by size:
4037 2497 181 163
BtsI GCAGTG_nn'
Cuts at: 187 219 279 876 2071 2376 2671 4121 187
Size: 32 60 597 1195 305 295 1450 2944
Fragments arranged by size:
2944 1450 1195 597 305 295 60 32
DraI TTT'AAA
Cuts at: 3877 3877
Size: 6878
DraIII CAC_nnn'GTG
Cuts at: 666 813 666
Size: 147 6731
DrdI GACnn_nn'nnGTC
Cuts at: 3544 4584 5928 3544
Size: 1040 1344 4494
Fragments arranged by size:
4494 1344 1040
EaeI y'GGCC_r
Cuts at: 512 692 1244 1620 3020 4463 4637 5028 5055
Size: 180 552 376 1400 1443 174 391 27
Cuts at: 5055 6400 512
Size: 1345 990
Fragments arranged by size:
1443 1400 1345 990 552 391 376 180
174 27
EagI C'GGCC_G
Cuts at: 4463 4463
Size: 6878
EarI CTCTTCn'nnn_
Cuts at: 557 1912 3857 4901 5111 557
Size: 1355 1945 1044 210 2324
Fragments arranged by size:
2324 1945 1355 1044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 119 2550 3181 5086 5828 5974 6688 119
Size: 2431 631 1905 742 146 714 309
Fragments arranged by size:
2431 1905 742 714 631 309 146
EcoICRI GAG'CTC
Cuts at: 1491 3890 1491
Size: 2399 4479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 481 756 1527 1590 1727 1936 2007 2024
Size: 277 275 771 63 137 209 71 17
Cuts at: 2024 2746 3115 3140 3616 3634 4703 5135 5488
Size: 722 369 25 476 18 1069 432 353
Cuts at: 5488 6150 204
Size: 662 932
Fragments arranged by size:
1069 932 771 722 662 476 432 369
353 277 275 209 137 71 63 25
18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 994 1180 2284 2869 994
Size: 186 1104 585 5003
Fragments arranged by size:
5003 1104 585 186
EcoO109I rG'GnC_Cy
Cuts at: 183 1185 1232 1417 1467 1574 2224 2551 2855
Size: 1002 47 185 50 107 650 327 304
Cuts at: 2855 3032 3297 4017 183
Size: 177 265 720 3044
Fragments arranged by size:
3044 1002 720 650 327 304 265 185
177 107 50 47
EcoRI G'AATT_C
Cuts at: 1082 3882 1082
Size: 2800 4078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 870 2280 2465 4897 870
Size: 1410 185 2432 2851
Fragments arranged by size:
2851 2432 1410 185
FspI TGC'GCA
Cuts at: 4659 4659
Size: 6878
HaeII r_GCGC'y
Cuts at: 805 1954 2692 2812 3211 4312 4560 5790 6561
Size: 1149 738 120 399 1101 248 1230 771
Cuts at: 6561 805
Size: 1122
Fragments arranged by size:
1230 1149 1122 1101 771 738 399 248
120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 895 927 1313 1345 3214 3246 3289
Size: 32 259 32 386 32 1869 32 43
Cuts at: 3289 3321 4720 4750 4752 4782 604
Size: 32 1399 30 2 30 2700
Fragments arranged by size:
2700 1869 1399 386 259 43 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3917 3917
Size: 6878
HindIII A'AGCT_T
Cuts at: 847 847
Size: 6878
KasI G'GCGC_C
Cuts at: 801 2808 3207 4308 4556 801
Size: 2007 399 1101 248 3123
Fragments arranged by size:
3123 2007 1101 399 248
KpnI G_GTAC'C
Cuts at: 3898 3898
Size: 6878
MluI A'CGCG_T
Cuts at: 6311 6311
Size: 6878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1316 3794 5637 5821 1316
Size: 2478 1843 184 2373
Fragments arranged by size:
2478 2373 1843 184
MscI TGG'CCA
Cuts at: 514 1246 4639 514
Size: 732 3393 2753
Fragments arranged by size:
3393 2753 732
MslI CAynn'nnrTG
Cuts at: 602 950 1223 3466 3718 4867 4994 602
Size: 348 273 2243 252 1149 127 2486
Fragments arranged by size:
2486 2243 1149 348 273 252 127
MspA1I CmG'CkG
Cuts at: 576 1346 1538 1756 1862 2574 2627 3093 3157
Size: 770 192 218 106 712 53 466 64
Cuts at: 3157 3564 3989 4303 4663 5447 5692 576
Size: 407 425 314 360 784 245 1762
Fragments arranged by size:
1762 784 770 712 466 425 407 360
314 245 218 192 106 64 53
NaeI GCC'GGC
Cuts at: 5059 5059
Size: 6878
NarI GG'CG_CC
Cuts at: 802 2809 3208 4309 4557 802
Size: 2007 399 1101 248 3123
Fragments arranged by size:
3123 2007 1101 399 248
NcoI C'CATG_G
Cuts at: 1366 1778 3023 3125 1366
Size: 412 1245 102 5119
Fragments arranged by size:
5119 1245 412 102
NdeI CA'TA_TG
Cuts at: 3774 3774
Size: 6878
NgoMIV G'CCGG_C
Cuts at: 5057 5057
Size: 6878
NsiI A_TGCA'T
Cuts at: 901 901
Size: 6878
NspI r_CATG'y
Cuts at: 867 3931 4431 6034 867
Size: 3064 500 1603 1711
Fragments arranged by size:
3064 1711 1603 500
PasI CC'CwG_GG
Cuts at: 1990 1990
Size: 6878
PciI A'CATG_T
Cuts at: 6030 6030
Size: 6878
PflMI CCAn_nnn'nTGG
Cuts at: 1143 2002 2597 1143
Size: 859 595 5424
Fragments arranged by size:
5424 859 595
PfoI T'CCnGG_A
Cuts at: 1136 1136
Size: 6878
PmeI GTTT'AAAC
Cuts at: 3877 3877
Size: 6878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5324 5356 5324
Size: 32 6846
PpuMI rG'GwC_Cy
Cuts at: 183 1185 1574 2855 3297 183
Size: 1002 389 1281 442 3764
Fragments arranged by size:
3764 1281 1002 442 389
PshAI GACnn'nnGTC
Cuts at: 3395 3395
Size: 6878
PspOMI G'GGCC_C
Cuts at: 1416 2223 2551 1416
Size: 807 328 5743
Fragments arranged by size:
5743 807 328
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1309 1341 1309
Size: 32 6846
PstI C_TGCA'G
Cuts at: 194 400 480 1268 1523 2336 3077 3092 3506
Size: 206 80 788 255 813 741 15 414
Cuts at: 3506 3925 4610 194
Size: 419 685 2462
Fragments arranged by size:
2462 813 788 741 685 419 414 255
206 80 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6878
PvuII CAG'CTG
Cuts at: 576 1346 1756 1862 2627 3093 3157 3564 4303
Size: 770 410 106 765 466 64 407 739
Cuts at: 4303 4663 576
Size: 360 2791
Fragments arranged by size:
2791 770 765 739 466 410 407 360
106 64
RsrII CG'GwC_CG
Cuts at: 5073 5073
Size: 6878
SacI G_AGCT'C
Cuts at: 1493 3892 1493
Size: 2399 4479
SalI G'TCGA_C
Cuts at: 3915 3915
Size: 6878
SanDI GG'GwC_CC
Cuts at: 1574 2855 1574
Size: 1281 5597
SapI GCTCTTCn'nnn_
Cuts at: 3857 4901 5111 3857
Size: 1044 210 5624
Fragments arranged by size:
5624 1044 210
SbfI CC_TGCA'GG
Cuts at: 3506 3925 3506
Size: 419 6459
ScaI AGT'ACT
Cuts at: 6395 6395
Size: 6878
SexAI A'CCwGG_T
Cuts at: 734 989 734
Size: 255 6623
SfcI C'TryA_G
Cuts at: 33 190 396 476 1264 1519 2121 2332 3073
Size: 157 206 80 788 255 602 211 741
Cuts at: 3073 3088 3158 3502 3622 3921 4606 5574 5765
Size: 15 70 344 120 299 685 968 191
Cuts at: 5765 33
Size: 1146
Fragments arranged by size:
1146 968 788 741 685 602 344 299
255 211 206 191 157 120 80 70
15
SfoI GGC'GCC
Cuts at: 803 2810 3209 4310 4558 803
Size: 2007 399 1101 248 3123
Fragments arranged by size:
3123 2007 1101 399 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6878
SmaI CCC'GGG
Cuts at: 3004 3900 3004
Size: 896 5982
SmlI C'TyrA_G
Cuts at: 890 2519 4033 4948 5662 5924 890
Size: 1629 1514 915 714 262 1844
Fragments arranged by size:
1844 1629 1514 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6878
SphI G_CATG'C
Cuts at: 3931 4431 3931
Size: 500 6378
SspI AAT'ATT
Cuts at: 3412 3762 3412
Size: 350 6528
StuI AGG'CCT
Cuts at: 2875 2875
Size: 6878
StyI C'CwwG_G
Cuts at: 152 491 1366 1778 2285 2405 2467 3023 3125
Size: 339 875 412 507 120 62 556 102
Cuts at: 3125 4012 4366 152
Size: 887 354 2664
Fragments arranged by size:
2664 887 875 556 507 412 354 339
120 102 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1129 4461 4594 5053 5262 1129
Size: 3332 133 459 209 2745
Fragments arranged by size:
3332 2745 459 209 133
TatI w'GTAC_w
Cuts at: 722 1325 6393 722
Size: 603 5068 1207
Fragments arranged by size:
5068 1207 603
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 1525 1557 6395 6427 599
Size: 32 894 32 4838 32 1050
Fragments arranged by size:
4838 1050 894 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1873 4675 1873
Size: 2802 4076
XbaI T'CTAG_A
Cuts at: 3909 3909
Size: 6878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 2139 4373 159
Size: 1980 2234 2664
Fragments arranged by size:
2664 2234 1980
XhoI C'TCGA_G
Cuts at: 2519 2519
Size: 6878
XmaI C'CCGG_G
Cuts at: 3002 3898 3002
Size: 896 5982
XmnI GAAnn'nnTTC
Cuts at: 913 2448 2460 3785 913
Size: 1535 12 1325 4006
Fragments arranged by size:
4006 1535 1325 12
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BbvCI BmtI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
PacI PmlI PsiI PspXI SacII SfiI SgrAI SnaBI
SrfI SwaI ZraI