Restriction map for
pF1KA0321
(Circular) (Six-base) MAPSORT of: pF1KA0321.seq Check: 7879 from: 1 to: 10730
pF1KA0321 10730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4653 7605 4653
Size: 2952 7778
AccI GT'mk_AC
Cuts at: 3129 5439 7768 3129
Size: 2310 2329 6091
Fragments arranged by size:
6091 2329 2310
Acc65I G'GTAC_C
Cuts at: 5535 7746 5535
Size: 2211 8519
AclI AA'CG_TT
Cuts at: 10570 10570
Size: 10730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 2701 3172 3286 3364 3558 4381 4427 5729
Size: 2448 471 114 78 194 823 46 1302
Cuts at: 5729 6397 6578 6915 7024 7044 7093 8555 8987
Size: 668 181 337 109 20 49 1462 432
Cuts at: 8987 9340 10002 253
Size: 353 662 981
Fragments arranged by size:
2448 1462 1302 981 823 668 662 471
432 353 337 194 181 114 109 78
49 46 20
AfeI AGC'GCT
Cuts at: 2722 2850 2722
Size: 128 10602
AflII C'TTAA_G
Cuts at: 4030 6985 4030
Size: 2955 7775
AflIII A'CryG_T
Cuts at: 2389 8082 9882 10163 2389
Size: 5693 1800 281 2956
Fragments arranged by size:
5693 2956 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 548 3230 548
Size: 2682 8048
AleI CACnn'nnGTG
Cuts at: 4408 4408
Size: 10730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4156 6221 7255 4156
Size: 2065 1034 7631
Fragments arranged by size:
7631 2065 1034
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 2695
Size: 32 10698
AlwNI CAG_nnn'CTG
Cuts at: 618 1122 1275 1564 1590 1750 1794 2664 3387
Size: 504 153 289 26 160 44 870 723
Cuts at: 3387 3581 3797 4266 5064 5217 5220 5332 5642
Size: 194 216 469 798 153 3 112 310
Cuts at: 5642 6054 6371 7380 7482 8035 9473 618
Size: 412 317 1009 102 553 1438 1875
Fragments arranged by size:
1875 1438 1009 870 798 723 553 504
469 412 317 310 289 216 194 160
153 153 112 102 44 26 3
ApaI G_GGCC'C
Cuts at: 727 1294 4395 727
Size: 567 3101 7062
Fragments arranged by size:
7062 3101 567
ApaLI G'TGCA_C
Cuts at: 9568 9568
Size: 10730
ApoI r'AATT_y
Cuts at: 1189 2152 5494 5881 7466 7734 10083 10339 1189
Size: 963 3342 387 1585 268 2349 256 1580
Fragments arranged by size:
3342 2349 1585 1580 963 387 268 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10730
AvaI C'yCGr_G
Cuts at: 371 466 984 1111 3933 4080 4166 4388 5374
Size: 95 518 127 2822 147 86 222 986
Cuts at: 5374 7694 7750 371
Size: 2320 56 3351
Fragments arranged by size:
3351 2822 2320 986 518 222 147 127
95 86 56
BamHI G'GATC_C
Cuts at: 5074 7755 5074
Size: 2681 8049
BanI G'GyrC_C
Cuts at: 735 3565 4140 5525 5535 7699 7746 8160 8408
Size: 2830 575 1385 10 2164 47 414 248
Cuts at: 8408 8443 735
Size: 35 3022
Fragments arranged by size:
3022 2830 2164 1385 575 414 248 47
35 10
BanII G_rGCy'C
Cuts at: 629 727 771 1294 1310 1341 1936 1966 2975
Size: 98 44 523 16 31 595 30 1009
Cuts at: 2975 3416 3622 3682 3809 3931 4235 4395 7744
Size: 441 206 60 127 122 304 160 3349
Cuts at: 7744 8774 629
Size: 1030 2585
Fragments arranged by size:
3349 2585 1030 1009 595 523 441 304
206 160 127 122 98 60 44 31
30 16
BbeI G_GCGC'C
Cuts at: 8164 8412 8164
Size: 248 10482
BbsI GAAGACnn'nnnn_
Cuts at: 266 440 1160 3008 3425 266
Size: 174 720 1848 417 7571
Fragments arranged by size:
7571 1848 720 417 174
BbvCI CC'TCA_GC
Cuts at: 218 1707 1907 1967 218
Size: 1489 200 60 8981
Fragments arranged by size:
8981 1489 200 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6475 6509 6475
Size: 34 10696
BciVI GTATCCnnnnn_n'
Cuts at: 6480 8628 9684 10730 6480
Size: 2148 1056 1046 6480
Fragments arranged by size:
6480 2148 1056 1046
BclI T'GATC_A
Cuts at: 3630 5700 8250 3630
Size: 2070 2550 6110
Fragments arranged by size:
6110 2550 2070
BfrBI ATG'CAT
Cuts at: 4519 4519
Size: 10730
BglI GCCn_nnn'nGGC
Cuts at: 2091 3748 5194 5621 2091
Size: 1657 1446 427 7200
Fragments arranged by size:
7200 1657 1446 427
BglII A'GATC_T
Cuts at: 1398 6191 7007 8245 10282 1398
Size: 4793 816 1238 2037 1846
Fragments arranged by size:
4793 2037 1846 1238 816
BlpI GC'TnA_GC
Cuts at: 886 2491 7842 886
Size: 1605 5351 3774
Fragments arranged by size:
5351 3774 1605
Bme1580I G_kGCm'C
Cuts at: 727 740 1294 3568 4395 5504 7671 7704 8355
Size: 13 554 2274 827 1109 2167 33 651
Cuts at: 8355 8448 9572 727
Size: 93 1124 1885
Fragments arranged by size:
2274 2167 1885 1124 1109 827 651 554
93 33 13
BmgBI CAC'GTC
Cuts at: 2392 2889 2392
Size: 497 10233
BmrI ACTGGGnnnn_n'
Cuts at: 385 1659 2310 2393 2687 4693 6265 6290 6970
Size: 1274 651 83 294 2006 1572 25 680
Cuts at: 6970 7665 7942 8023 8358 10502 385
Size: 695 277 81 335 2144 613
Fragments arranged by size:
2144 2006 1572 1274 695 680 651 613
335 294 277 83 81 25
BmtI G_CTAG'C
Cuts at: 1033 1033
Size: 10730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 560 614 5277 6557 6988 6990 9097 560
Size: 54 4663 1280 431 2 2107 2193
Fragments arranged by size:
4663 2193 2107 1280 431 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 643 1252 1366 1375 1541 1843 2920 2936
Size: 267 609 114 9 166 302 1077 16
Cuts at: 2936 3449 3493 4588 4600 5173 5194 5418 6689
Size: 513 44 1095 12 573 21 224 1271
Cuts at: 6689 7504 7701 376
Size: 815 197 3405
Fragments arranged by size:
3405 1271 1095 1077 815 609 573 513
302 267 224 197 166 114 44 21
16 12 9
Bpu10I CC'TnA_GC
Cuts at: 218 1016 1090 1565 1573 1707 1907 1967 2253
Size: 798 74 475 8 134 200 60 286
Cuts at: 2253 3322 4459 5099 6220 218
Size: 1069 1137 640 1121 4728
Fragments arranged by size:
4728 1137 1121 1069 798 640 475 286
200 134 74 60 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 655 1480 4743 6399 7906 8785 9499 9797
Size: 222 825 3263 1656 1507 879 714 298
Cuts at: 9797 433
Size: 1366
Fragments arranged by size:
3263 1656 1507 1366 879 825 714 298
222
BsaI GGTCTCn'nnnn_
Cuts at: 475 2912 5371 6273 10434 475
Size: 2437 2459 902 4161 771
Fragments arranged by size:
4161 2459 2437 902 771
BsaAI yAC'GTr
Cuts at: 510 3144 6174 6648 8083 510
Size: 2634 3030 474 1435 3157
Fragments arranged by size:
3157 3030 2634 1435 474
BsaBI GATnn'nnATC
Cuts at: 6 5394 8244 10281 6
Size: 5388 2850 2037 455
Fragments arranged by size:
5388 2850 2037 455
BsaHI Gr'CG_yC
Cuts at: 5601 8161 8409 9111 10607 5601
Size: 2560 248 702 1496 5724
Fragments arranged by size:
5724 2560 1496 702 248
BsaWI w'CCGG_w
Cuts at: 401 999 5538 7919 8440 9118 9529 9676 9950
Size: 598 4539 2381 521 678 411 147 274
Cuts at: 9950 401
Size: 1181
Fragments arranged by size:
4539 2381 1181 678 598 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 1645 1675 1720 1750 4719 4749 349
Size: 30 1266 30 45 30 2969 30 6330
Fragments arranged by size:
6330 2969 1266 45 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 536 652 889 1300 1728 2371 3281 3413
Size: 52 116 237 411 428 643 910 132
Cuts at: 3413 3496 3837 3966 4002 4740 5411 5752 5869
Size: 83 341 129 36 738 671 341 117
Cuts at: 5869 7429 484
Size: 1560 3785
Fragments arranged by size:
3785 1560 910 738 671 643 428 411
341 341 237 132 129 117 116 83
52 36
BseYI C'CCAG_C
Cuts at: 602 1053 1529 3316 3359 3878 3999 4102 5480
Size: 451 476 1787 43 519 121 103 1378
Cuts at: 5480 5505 5902 6806 7156 7242 8155 8913 9578
Size: 25 397 904 350 86 913 758 665
Cuts at: 9578 9986 602
Size: 408 1346
Fragments arranged by size:
1787 1378 1346 913 904 758 665 519
476 451 408 397 350 121 103 86
43 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 883 1261 1818 2530 3303 3508 5221 5655
Size: 365 378 557 712 773 205 1713 434
Cuts at: 5655 5920 6335 6445 6592 6664 7639 518
Size: 265 415 110 147 72 975 3609
Fragments arranged by size:
3609 1713 975 773 712 557 434 415
378 365 265 205 147 110 72
BsiEI CG_ry'CG
Cuts at: 104 8272 8318 9548 104
Size: 8168 46 1230 1286
Fragments arranged by size:
8168 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 578 629 1310 1442 1571 2497 2762 3622 3682
Size: 51 681 132 129 926 265 860 60
Cuts at: 3682 3931 5582 5978 7744 8522 8712 9572 578
Size: 249 1651 396 1766 778 190 860 1736
Fragments arranged by size:
1766 1736 1651 926 860 860 778 681
396 265 249 190 132 129 60 51
BsmI GAATG_Cn'
Cuts at: 178 684 3164 6598 178
Size: 506 2480 3434 4310
Fragments arranged by size:
4310 3434 2480 506
BsmBI CGTCTCn'nnnn_
Cuts at: 556 556
Size: 10730
Bsp1286I G_dGCh'C
Cuts at: 578 629 727 740 771 1294 1310 1341 1442
Size: 51 98 13 31 523 16 31 101
Cuts at: 1442 1571 1936 1966 2497 2762 2975 3416 3568
Size: 129 365 30 531 265 213 441 152
Cuts at: 3568 3622 3682 3809 3931 4235 4395 5504 5582
Size: 54 60 127 122 304 160 1109 78
Cuts at: 5582 5978 7671 7704 7744 8355 8448 8522 8712
Size: 396 1693 33 40 611 93 74 190
Cuts at: 8712 8774 9572 578
Size: 62 798 1736
Fragments arranged by size:
1736 1693 1109 798 611 531 523 441
396 365 304 265 213 190 160 152
129 127 122 101 98 93 78 74
62 60 54 51 40 33 31 31
30 16 13
BspEI T'CCGG_A
Cuts at: 401 401
Size: 10730
BspHI T'CATG_A
Cuts at: 4757 4887 6041 6569 9162 4757
Size: 130 1154 528 2593 6325
Fragments arranged by size:
6325 2593 1154 528 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3076 4149 4653 6614 7605 7780 7994 8296 8677
Size: 1073 504 1961 991 175 214 302 381
Cuts at: 8677 3076
Size: 5129
Fragments arranged by size:
5129 1961 1073 991 504 381 302 214
175
BsrBI CCG'CTC
Cuts at: 50 879 1020 2825 9022 9076 10560 10725 50
Size: 829 141 1805 6197 54 1484 165 55
Fragments arranged by size:
6197 1805 1484 829 165 141 55 54
BsrDI GCAATG_nn'
Cuts at: 706 1031 1846 4328 5918 5948 8642 706
Size: 325 815 2482 1590 30 2694 2794
Fragments arranged by size:
2794 2694 2482 1590 815 325 30
BsrFI r'CCGG_y
Cuts at: 5624 6085 8728 8909 5624
Size: 461 2643 181 7445
Fragments arranged by size:
7445 2643 461 181
BssSI C'ACGA_G
Cuts at: 5690 9001 9709 5690
Size: 3311 708 6711
Fragments arranged by size:
6711 3311 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 4908 5567 5642 6323 8107 1552
Size: 3356 659 75 681 1784 4175
Fragments arranged by size:
4175 3356 1784 681 659 75
BstBI TT'CG_AA
Cuts at: 699 9091 699
Size: 8392 2338
BstEII G'GTnAC_C
Cuts at: 5959 5959
Size: 10730
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 5023 2440
Size: 2583 8147
BstYI r'GATC_y
Cuts at: 1 582 1011 1398 3491 5074 5370 5844 6191
Size: 581 429 387 2093 1583 296 474 347
Cuts at: 6191 7007 7370 7755 8222 8245 8580 8826 9132
Size: 816 363 385 467 23 335 246 306
Cuts at: 9132 9144 9230 10282 1
Size: 12 86 1052 449
Fragments arranged by size:
2093 1583 1052 816 581 474 467 449
429 387 385 363 347 335 306 296
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 5440 5440
Size: 10730
Bsu36I CC'TnA_GG
Cuts at: 589 1312 1477 1895 1938 2173 6502 589
Size: 723 165 418 43 235 4329 4817
Fragments arranged by size:
4817 4329 723 418 235 165 43
BtgI C'CryG_G
Cuts at: 3020 3277 7704 3020
Size: 257 4427 6046
Fragments arranged by size:
6046 4427 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8680 8861 9024 8680
Size: 181 163 10386
Fragments arranged by size:
10386 181 163
BtsI GCAGTG_nn'
Cuts at: 1233 2611 2823 2975 3894 4277 5263 5976 6344
Size: 1378 212 152 919 383 986 713 368
Cuts at: 6344 6554 7973 1233
Size: 210 1419 3990
Fragments arranged by size:
3990 1419 1378 986 919 713 383 368
212 210 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7587 7622 7587
Size: 35 10695
DraI TTT'AAA
Cuts at: 7729 7729
Size: 10730
DraIII CAC_nnn'GTG
Cuts at: 496 1437 2331 3486 4934 496
Size: 941 894 1155 1448 6292
Fragments arranged by size:
6292 1448 1155 941 894
DrdI GACnn_nn'nnGTC
Cuts at: 4612 8436 9780 4612
Size: 3824 1344 5562
Fragments arranged by size:
5562 3824 1344
EaeI y'GGCC_r
Cuts at: 933 1775 2442 2532 2820 4128 4373 4979 4997
Size: 842 667 90 288 1308 245 606 18
Cuts at: 4997 5622 6087 6629 8315 8489 8880 8907 10252
Size: 625 465 542 1686 174 391 27 1345
Cuts at: 10252 933
Size: 1411
Fragments arranged by size:
1686 1411 1345 1308 842 667 625 606
542 465 391 288 245 174 90 27
18
EagI C'GGCC_G
Cuts at: 8315 8315
Size: 10730
EarI CTCTTCn'nnn_
Cuts at: 1765 1924 3687 4793 4875 6604 6680 8753 8963
Size: 159 1763 1106 82 1729 76 2073 210
Cuts at: 8963 1765
Size: 3532
Fragments arranged by size:
3532 2073 1763 1729 1106 210 159 82
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 4669 7460 8938 9680 9826 10540 2395
Size: 2274 2791 1478 742 146 714 2585
Fragments arranged by size:
2791 2585 2274 1478 742 714 146
EcoICRI GAG'CTC
Cuts at: 627 1308 3620 3680 3929 7742 627
Size: 681 2312 60 249 3813 3615
Fragments arranged by size:
3813 3615 2312 681 249 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 376 643 1252 1366 1375 1541 1843 2701
Size: 123 267 609 114 9 166 302 858
Cuts at: 2701 2920 2936 3172 3286 3364 3449 3493 3558
Size: 219 16 236 114 78 85 44 65
Cuts at: 3558 4381 4427 4588 4600 5173 5194 5418 5729
Size: 823 46 161 12 573 21 224 311
Cuts at: 5729 6397 6578 6689 6915 7024 7044 7093 7504
Size: 668 181 111 226 109 20 49 411
Cuts at: 7504 7701 8555 8987 9340 10002 253
Size: 197 854 432 353 662 981
Fragments arranged by size:
981 858 854 823 668 662 609 573
432 411 353 311 302 267 236 226
224 219 197 181 166 161 123 114
114 111 109 85 78 65 49 46
44 21 20 16 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 634 826 871 1480 1693 2172 2989 3325 3981
Size: 192 45 609 213 479 817 336 656
Cuts at: 3981 7495 634
Size: 3514 3869
Fragments arranged by size:
3869 3514 817 656 609 479 336 213
192 45
EcoO109I rG'GnC_Cy
Cuts at: 615 723 724 915 1196 1283 1290 1350 1524
Size: 108 1 191 281 87 7 60 174
Cuts at: 1524 1698 1949 2083 2165 3271 3375 4392 4743
Size: 174 251 134 82 1106 104 1017 351
Cuts at: 4743 5898 5997 6461 7160 7488 7869 615
Size: 1155 99 464 699 328 381 3476
Fragments arranged by size:
3476 1155 1106 1017 699 464 381 351
328 281 251 191 174 174 134 108
104 99 87 82 60 7 1
EcoRI G'AATT_C
Cuts at: 1189 7466 7734 1189
Size: 6277 268 4185
Fragments arranged by size:
6277 4185 268
EcoRV GAT'ATC
Cuts at: 243 243
Size: 10730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 283 6538 8749 283
Size: 6255 2211 2264
Fragments arranged by size:
6255 2264 2211
FspI TGC'GCA
Cuts at: 8511 8511
Size: 10730
HaeII r_GCGC'y
Cuts at: 2724 2852 4182 5065 5909 6065 8164 8412 9642
Size: 128 1330 883 844 156 2099 248 1230
Cuts at: 9642 10413 2724
Size: 771 3041
Fragments arranged by size:
3041 2099 1330 1230 883 844 771 248
156 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 534 566 1417 1449 1566 1598 1675 1690 1707
Size: 32 851 32 117 32 77 15 17
Cuts at: 1707 1720 1722 1752 2909 2941 3216 3248 3483
Size: 13 2 30 1157 32 275 32 235
Cuts at: 3483 3515 4732 4764 5308 5340 5829 5861 8572
Size: 32 1217 32 544 32 489 32 2711
Cuts at: 8572 8602 8604 8634 534
Size: 30 2 30 2630
Fragments arranged by size:
2711 2630 1217 1157 851 544 489 275
235 117 77 32 32 32 32 32
32 32 32 32 30 30 30 17
15 13 2 2
HincII GTy'rAC
Cuts at: 1786 3130 7769 1786
Size: 1344 4639 4747
Fragments arranged by size:
4747 4639 1344
HindIII A'AGCT_T
Cuts at: 409 409
Size: 10730
KasI G'GCGC_C
Cuts at: 8160 8408 8160
Size: 248 10482
KpnI G_GTAC'C
Cuts at: 5539 7750 5539
Size: 2211 8519
MluI A'CGCG_T
Cuts at: 10163 10163
Size: 10730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2398 5067 5205 6756 6817 9489 9673 2398
Size: 2669 138 1551 61 2672 184 3455
Fragments arranged by size:
3455 2672 2669 1551 184 138 61
MscI TGG'CCA
Cuts at: 935 4375 4981 4999 6631 8491 935
Size: 3440 606 18 1632 1860 3174
Fragments arranged by size:
3440 3174 1860 1632 606 18
MslI CAynn'nnrTG
Cuts at: 207 941 2587 2929 4408 4762 5021 5176 7216
Size: 734 1646 342 1479 354 259 155 2040
Cuts at: 7216 7511 8719 8846 207
Size: 295 1208 127 2091
Fragments arranged by size:
2091 2040 1646 1479 1208 734 354 342
295 259 155 127
MspA1I CmG'CkG
Cuts at: 890 3794 5160 5217 5618 5967 6051 6205 6402
Size: 2904 1366 57 401 349 84 154 197
Cuts at: 6402 6659 7081 7113 7305 7640 7841 8155 8515
Size: 257 422 32 192 335 201 314 360
Cuts at: 8515 9299 9544 890
Size: 784 245 2076
Fragments arranged by size:
2904 2076 1366 784 422 401 360 349
335 314 257 245 201 197 192 154
84 57 32
NaeI GCC'GGC
Cuts at: 5626 6087 8911 5626
Size: 461 2824 7445
Fragments arranged by size:
7445 2824 461
NarI GG'CG_CC
Cuts at: 8161 8409 8161
Size: 248 10482
NcoI C'CATG_G
Cuts at: 3020 7704 3020
Size: 4684 6046
NgoMIV G'CCGG_C
Cuts at: 5624 6085 8909 5624
Size: 461 2824 7445
Fragments arranged by size:
7445 2824 461
NheI G'CTAG_C
Cuts at: 1029 1029
Size: 10730
NsiI A_TGCA'T
Cuts at: 4521 4521
Size: 10730
NspI r_CATG'y
Cuts at: 212 4478 6310 7783 8283 9886 212
Size: 4266 1832 1473 500 1603 1056
Fragments arranged by size:
4266 1832 1603 1473 1056 500
PasI CC'CwG_GG
Cuts at: 742 1287 2010 2080 742
Size: 545 723 70 9392
Fragments arranged by size:
9392 723 545 70
PciI A'CATG_T
Cuts at: 9882 9882
Size: 10730
PflMI CCAn_nnn'nTGG
Cuts at: 1168 2745 4382 6102 6234 7347 7482 1168
Size: 1577 1637 1720 132 1113 135 4416
Fragments arranged by size:
4416 1720 1637 1577 1113 135 132
PfoI T'CCnGG_A
Cuts at: 1352 2936 7717 1352
Size: 1584 4781 4365
Fragments arranged by size:
4781 4365 1584
PmeI GTTT'AAAC
Cuts at: 7729 7729
Size: 10730
PmlI CAC'GTG
Cuts at: 510 6174 510
Size: 5664 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9176 9208 9176
Size: 32 10698
PpuMI rG'GwC_Cy
Cuts at: 1283 1350 1524 1698 1949 3375 4743 7160 1283
Size: 67 174 174 251 1426 1368 2417 4853
Fragments arranged by size:
4853 2417 1426 1368 251 174 174 67
PshAI GACnn'nnGTC
Cuts at: 3008 5038 3008
Size: 2030 8700
PspOMI G'GGCC_C
Cuts at: 723 1290 4391 723
Size: 567 3101 7062
Fragments arranged by size:
7062 3101 567
PspXI vC'TCGA_Gb
Cuts at: 466 1111 466
Size: 645 10085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6565 6597 6565
Size: 32 10698
PstI C_TGCA'G
Cuts at: 677 850 2276 2856 3586 3600 4155 4664 5370
Size: 173 1426 580 730 14 555 509 706
Cuts at: 5370 5575 5677 5986 6210 6881 7080 7118 7295
Size: 205 102 309 224 671 199 38 177
Cuts at: 7295 7330 7777 8462 677
Size: 35 447 685 2945
Fragments arranged by size:
2945 1426 730 706 685 671 580 555
509 447 309 224 205 199 177 173
102 38 35 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10730
PvuII CAG'CTG
Cuts at: 890 3794 5160 5217 5967 6051 6205 6402 6659
Size: 2904 1366 57 750 84 154 197 257
Cuts at: 6659 7081 7113 7305 8155 8515 890
Size: 422 32 192 850 360 3105
Fragments arranged by size:
3105 2904 1366 850 750 422 360 257
197 192 154 84 57 32
RsrII CG'GwC_CG
Cuts at: 8925 8925
Size: 10730
SacI G_AGCT'C
Cuts at: 629 1310 3622 3682 3931 7744 629
Size: 681 2312 60 249 3813 3615
Fragments arranged by size:
3813 3615 2312 681 249 60
SalI G'TCGA_C
Cuts at: 3128 7767 3128
Size: 4639 6091
SapI GCTCTTCn'nnn_
Cuts at: 1924 3687 6680 8753 8963 1924
Size: 1763 2993 2073 210 3691
Fragments arranged by size:
3691 2993 2073 1763 210
SbfI CC_TGCA'GG
Cuts at: 850 7777 850
Size: 6927 3803
ScaI AGT'ACT
Cuts at: 6247 6817 10247 6247
Size: 570 3430 6730
Fragments arranged by size:
6730 3430 570
SexAI A'CCwGG_T
Cuts at: 7129 7129
Size: 10730
SfcI C'TryA_G
Cuts at: 33 223 673 846 977 1712 2272 2833 2852
Size: 190 450 173 131 735 560 561 19
Cuts at: 2852 3582 3596 4151 4543 4639 4660 5366 5571
Size: 730 14 555 392 96 21 706 205
Cuts at: 5571 5673 5709 5982 6206 6615 6877 7076 7114
Size: 102 36 273 224 409 262 199 38
Cuts at: 7114 7291 7326 7773 8458 9426 9617 33
Size: 177 35 447 685 968 191 1146
Fragments arranged by size:
1146 968 735 730 706 685 561 560
555 450 447 409 392 273 262 224
205 199 191 190 177 173 131 102
96 38 36 35 21 19 14
SfoI GGC'GCC
Cuts at: 8162 8410 8162
Size: 248 10482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10730
SmaI CCC'GGG
Cuts at: 373 4168 4390 7696 7752 373
Size: 3795 222 3306 56 3351
Fragments arranged by size:
3795 3351 3306 222 56
SmlI C'TyrA_G
Cuts at: 412 466 634 1111 1495 4030 4722 6414 6985
Size: 54 168 477 384 2535 692 1692 571
Cuts at: 6985 7885 8800 9514 9776 412
Size: 900 915 714 262 1366
Fragments arranged by size:
2535 1692 1366 915 900 714 692 571
477 384 262 168 54
SpeI A'CTAG_T
Cuts at: 67 2122 67
Size: 2055 8675
SphI G_CATG'C
Cuts at: 6310 7783 8283 6310
Size: 1473 500 8757
Fragments arranged by size:
8757 1473 500
SspI AAT'ATT
Cuts at: 6147 6147
Size: 10730
StuI AGG'CCT
Cuts at: 844 4210 844
Size: 3366 7364
StyI C'CwwG_G
Cuts at: 441 526 1520 2297 3020 3982 4019 4367 5993
Size: 85 994 777 723 962 37 348 1626
Cuts at: 5993 6797 6992 7704 7864 8218 441
Size: 804 195 712 160 354 2953
Fragments arranged by size:
2953 1626 994 962 804 777 723 712
354 348 195 160 85 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 868 1892 2706 5029 8313 8446 8905 9114 868
Size: 1024 814 2323 3284 133 459 209 2484
Fragments arranged by size:
3284 2484 2323 1024 814 459 209 133
TatI w'GTAC_w
Cuts at: 351 1215 2476 4341 6245 6815 7361 10245 351
Size: 864 1261 1865 1904 570 546 2884 836
Fragments arranged by size:
2884 1904 1865 1261 864 836 570 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1645 1677 4719 4751 10247 10279 1645
Size: 32 3042 32 5496 32 2096
Fragments arranged by size:
5496 3042 2096 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8527 8527
Size: 10730
XbaI T'CTAG_A
Cuts at: 1008 4770 4872 5763 7761 1008
Size: 3762 102 891 1998 3977
Fragments arranged by size:
3977 3762 1998 891 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 5655 6012 8225 1061
Size: 4594 357 2213 3566
Fragments arranged by size:
4594 3566 2213 357
XhoI C'TCGA_G
Cuts at: 466 1111 466
Size: 645 10085
XmaI C'CCGG_G
Cuts at: 371 4166 4388 7694 7750 371
Size: 3795 222 3306 56 3351
Fragments arranged by size:
3795 3351 3306 222 56
XmnI GAAnn'nnTTC
Cuts at: 1490 3299 6674 1490
Size: 1809 3375 5546
Fragments arranged by size:
5546 3375 1809
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AgeI AscI AvrII BaeI BsiWI BsrGI BssHII
ClaI FseI FspAI HpaI MfeI NdeI NotI NruI
PacI PsiI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI ZraI